Next Article in Journal
Toward Sensor-Based Context Aware Systems
Next Article in Special Issue
Biomarker Discovery by Novel Sensors Based on Nanoproteomics Approaches
Previous Article in Journal
FPGA Implementation for Real-Time Background Subtraction Based on Horprasert Model
Previous Article in Special Issue
Serological Thymidine Kinase 1 is a Biomarker for Early Detection of Tumours—A Health Screening Study on 35,365 People, Using a Sensitive Chemiluminescent Dot Blot Assay
Article Menu

Export Article

Open AccessReview
Sensors 2012, 12(1), 612-631;

Aptamers and Their Biological Applications

Department of Chemistry, Pohang University of Science and Technology, San31, Hyoja-dong, Pohang, Gyeongbuk 790-784, Korea
Author to whom correspondence should be addressed.
Received: 29 November 2011 / Revised: 31 December 2011 / Accepted: 4 January 2012 / Published: 9 January 2012
(This article belongs to the Special Issue Biomarkers and Nanosensors: New Approaches for Biology and Medicine)
Full-Text   |   PDF [922 KB, uploaded 21 June 2014]


Recently, aptamers have attracted the attention of many scientists, because they not only have all of the advantages of antibodies, but also have unique merits, such as thermal stability, low cost, and unlimited applications. In this review, we present the reasons why aptamers are known as alternatives to antibodies. Furthermore, several types of in vitro selection processes, including nitrocellulose membrane filtration, affinity chromatography, magnetic bead, and capillary electrophoresis-based selection methods, are explained in detail. We also introduce various applications of aptamers for the diagnosis of diseases and detection of small molecules. Numerous analytical techniques, such as electrochemical, colorimetric, optical, and mass-sensitive methods, can be utilized to detect targets, due to convenient modifications and the stability of aptamers. Finally, several medical and analytical applications of aptamers are presented. In summary, aptamers are promising materials for diverse areas, not just as alternatives to antibodies, but as the core components of medical and analytical equipment. View Full-Text
Keywords: aptamer; SELEX; in vitro selection; aptasensor; diagnosis; biosensor aptamer; SELEX; in vitro selection; aptasensor; diagnosis; biosensor
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Song, K.-M.; Lee, S.; Ban, C. Aptamers and Their Biological Applications. Sensors 2012, 12, 612-631.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top