Correction: Barszczewska-Pietraszek et al. Polθ Inhibitor (ART558) Demonstrates a Synthetic Lethal Effect with PARP and RAD52 Inhibitors in Glioblastoma Cells. Int. J. Mol. Sci. 2024, 25, 9134

Barszczewska-Pietraszek, Gabriela; Czarny, Piotr; Drzewiecka, Małgorzata; Błaszczyk, Maciej; Radek, Maciej; Synowiec, Ewelina; Wigner-Jeziorska, Paulina; Sitarek, Przemysław; Szemraj, Janusz; Skorski, Tomasz; Śliwiński, Tomasz

doi:10.3390/ijms27031327

In the original publication [1], there was a mistake in Figure 3 as published. The same dot plot was used for Figure 3E A + B and 3E A + L, although it is valid only for Figure 3E A + B. This oversight occurred inadvertently due to the processing of a large dataset. The corrected Figure 3 appears below. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.

Figure 3. Proapoptotic effect of DNA repair protein inhibition and an alkylating agent and their combinations on the GBM21 cancer cell line. (A)—variant with PARPi, (C)—variant with RAD52i and normal NHA (B)—variant with PARPi, (D)—variant with RAD52i cells, shown as a percentage of cells in the corresponding stage in graphs and representative dot-plots (E)—GBM21, (F)—NHA. Cells were stained with propidium iodide (PI) and annexin V. Annexin V has strong affinity to phosphatidylserine, which appears on the cell’s surface during early apoptosis, while propidium iodide binds to DNA by penetrating through the fragmented cell membrane, which is characteristic of necrosis and the late stages of apoptosis. Three independent experiments were performed, and the results are shown as the mean ± standard error of the mean (SEM). ** p ≤ 0.01, *** p-value ≤ 0.001; A—ART558, B—BMN673, L—L-OH-DOPA, T—TMZ. C−−—Living cells, C+−—Early Apoptotic cells, C++—Late Apoptotic cells, C−+—Necrotic cells.

Reference

Barszczewska-Pietraszek, G.; Czarny, P.; Drzewiecka, M.; Błaszczyk, M.; Radek, M.; Synowiec, E.; Wigner-Jeziorska, P.; Sitarek, P.; Szemraj, J.; Skorski, T.; et al. Polθ Inhibitor (ART558) Demonstrates a Synthetic Lethal Effect with PARP and RAD52 Inhibitors in Glioblastoma Cells. Int. J. Mol. Sci. 2024, 25, 9134. [Google Scholar] [CrossRef] [Scilit] [PubMed]

Figure 3. Proapoptotic effect of DNA repair protein inhibition and an alkylating agent and their combinations on the GBM21 cancer cell line. (A)—variant with PARPi, (C)—variant with RAD52i and normal NHA (B)—variant with PARPi, (D)—variant with RAD52i cells, shown as a percentage of cells in the corresponding stage in graphs and representative dot-plots (E)—GBM21, (F)—NHA. Cells were stained with propidium iodide (PI) and annexin V. Annexin V has strong affinity to phosphatidylserine, which appears on the cell’s surface during early apoptosis, while propidium iodide binds to DNA by penetrating through the fragmented cell membrane, which is characteristic of necrosis and the late stages of apoptosis. Three independent experiments were performed, and the results are shown as the mean ± standard error of the mean (SEM). ** p ≤ 0.01, *** p-value ≤ 0.001; A—ART558, B—BMN673, L—L-OH-DOPA, T—TMZ. C−−—Living cells, C+−—Early Apoptotic cells, C++—Late Apoptotic cells, C−+—Necrotic cells.

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