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Article

Functional Characterization of the MeSSIII-1 Gene and Its Promoter from Cassava

by
Xiao-Hua Lu
1,†,
Ya-Jie Wang
2,†,
Xing-Hou Zhen
1,
Hui Yu
2,
Mu Pan
1,
Dong-Qing Fu
3,
Rui-Mei Li
2,
Jiao Liu
2,
Hai-Yan Luo
4,
Xin-Wen Hu
1,
Yuan Yao
2,* and
Jian-Chun Guo
2,*
1
National Key Laboratory for Tropical Crop Breeding, School of Life and Health Sciences, Hainan University, Haikou 570228, China
2
National Key Laboratory for Tropical Crop Breeding, Sanya Research Institute, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
3
School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
4
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(9), 4711; https://doi.org/10.3390/ijms25094711
Submission received: 22 March 2024 / Revised: 21 April 2024 / Accepted: 23 April 2024 / Published: 26 April 2024
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Soluble starch synthases (SSs) play important roles in the synthesis of cassava starch. However, the expression characteristics of the cassava SSs genes have not been elucidated. In this study, the MeSSIII-1 gene and its promoter, from SC8 cassava cultivars, were respectively isolated by PCR amplification. MeSSIII-1 protein was localized to the chloroplasts. qRT-PCR analysis revealed that the MeSSIII-1 gene was expressed in almost all tissues tested, and the expression in mature leaves was 18.9 times more than that in tuber roots. MeSSIII-1 expression was induced by methyljasmonate (MeJA), abscisic acid (ABA), and ethylene (ET) hormones in cassava. MeSSIII-1 expression patterns were further confirmed in proMeSSIII-1 transgenic cassava. The promoter deletion analysis showed that the −264 bp to −1 bp MeSSIII-1 promoter has basal activity. The range from −1228 bp to −987 bp and −488 bp to −264 bp significantly enhance promoter activity. The regions from −987 bp to −747 bp and −747 bp to −488 bp have repressive activity. These findings will provide an important reference for research on the potential function and transcriptional regulation mechanisms of the MeSSIII-1 gene and for further in-depth exploration of the regulatory network of its internal functional elements.
Keywords: cassava; starch synthase; MeSSIII-1; promoter; hormones; starch synthesis cassava; starch synthase; MeSSIII-1; promoter; hormones; starch synthesis

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MDPI and ACS Style

Lu, X.-H.; Wang, Y.-J.; Zhen, X.-H.; Yu, H.; Pan, M.; Fu, D.-Q.; Li, R.-M.; Liu, J.; Luo, H.-Y.; Hu, X.-W.; et al. Functional Characterization of the MeSSIII-1 Gene and Its Promoter from Cassava. Int. J. Mol. Sci. 2024, 25, 4711. https://doi.org/10.3390/ijms25094711

AMA Style

Lu X-H, Wang Y-J, Zhen X-H, Yu H, Pan M, Fu D-Q, Li R-M, Liu J, Luo H-Y, Hu X-W, et al. Functional Characterization of the MeSSIII-1 Gene and Its Promoter from Cassava. International Journal of Molecular Sciences. 2024; 25(9):4711. https://doi.org/10.3390/ijms25094711

Chicago/Turabian Style

Lu, Xiao-Hua, Ya-Jie Wang, Xing-Hou Zhen, Hui Yu, Mu Pan, Dong-Qing Fu, Rui-Mei Li, Jiao Liu, Hai-Yan Luo, Xin-Wen Hu, and et al. 2024. "Functional Characterization of the MeSSIII-1 Gene and Its Promoter from Cassava" International Journal of Molecular Sciences 25, no. 9: 4711. https://doi.org/10.3390/ijms25094711

APA Style

Lu, X.-H., Wang, Y.-J., Zhen, X.-H., Yu, H., Pan, M., Fu, D.-Q., Li, R.-M., Liu, J., Luo, H.-Y., Hu, X.-W., Yao, Y., & Guo, J.-C. (2024). Functional Characterization of the MeSSIII-1 Gene and Its Promoter from Cassava. International Journal of Molecular Sciences, 25(9), 4711. https://doi.org/10.3390/ijms25094711

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