Next Article in Journal
Electrical Stimulation of Human Adipose-Derived Mesenchymal Stem Cells on O2 Plasma-Treated ITO Glass Promotes Osteogenic Differentiation
Next Article in Special Issue
Reduced IQGAP2 Promotes Bladder Cancer through Regulation of MAPK/ERK Pathway and Cytokines
Previous Article in Journal
Accuracy of FIB-4 to Detect Elevated Liver Stiffness Measurements in Patients with Non-Alcoholic Fatty Liver Disease: A Cross-Sectional Study in Referral Centers
Previous Article in Special Issue
Prediction of Response to Cisplatin-Based Neoadjuvant Chemotherapy of Muscle-Invasive Bladder Cancer Patients by Molecular Subtyping including KRT and FGFR Target Gene Assessment
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 23
Issue 20
10.3390/ijms232012488
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Response of the Urothelial Carcinoma Cell Lines to Cisplatin

by
Andrea Holíčková
1,
Jan Roška
1,
Eveline Órásová
1,2,
Vladimíra Bruderová
1,
Patrik Palacka
3,4,
Dana Jurkovičová
1,† and
Miroslav Chovanec
1,*,†
1
Biomedical Research Center, Department of Genetics, Cancer Research Institute, Dúbravská cesta 9, 845 05 Bratislava, Slovakia
2
Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská Dolina, Ilkovičova 6, 842 15 Bratislava, Slovakia
3
National Cancer Institute, Klenová 1, 833 10 Bratislava, Slovakia
4
2nd Department of Oncology, Faculty of Medicine, Comenius University in Bratislava, Klenová 1, 833 10 Bratislava, Slovakia
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2022, 23(20), 12488; https://doi.org/10.3390/ijms232012488
Submission received: 9 August 2022 / Revised: 15 October 2022 / Accepted: 16 October 2022 / Published: 18 October 2022
(This article belongs to the Special Issue Molecular Research on Bladder Cancer)
Browse Figures
Versions Notes

Abstract

Cisplatin (CDDP)-based chemotherapy is the standard of care in patients with muscle-invasive bladder cancer. However, in a large number of cases, the disease becomes resistant or does not respond to CDDP, and thus progresses and disseminates. In such cases, prognosis of patients is very poor. CDDP manifests its cytotoxic effects mainly through DNA damage induction. Hence, response to CDDP is mainly dependent on DNA damage repair and tolerance mechanisms. Herein, we have examined CDDP response in a panel of the urothelial carcinoma cell (UCC) lines. We characterized these cell lines with regard to viability after CDDP treatment, as well as kinetics of induction and repair of CDDP-induced DNA damage. We demonstrate that repair of CDDP-induced DNA lesions correlates, at least to some extent, with CDDP sensitivity. Furthermore, we monitored expression of the key genes involved in selected DNA repair and tolerance mechanisms, nucleotide excision repair, homologous recombination and translesion DNA synthesis, and show that it differs in the UCC lines and positively correlates with CDDP resistance. Our data indicate that CDDP response in the UCC lines is dependent on DNA damage repair and tolerance factors, which may, therefore, represent valuable therapeutic targets in this malignancy.
Keywords: bladder cancer; cisplatin; DNA damage repair and tolerance; nucleotide excision repair; homologous recombination; translesion DNA synthesis bladder cancer; cisplatin; DNA damage repair and tolerance; nucleotide excision repair; homologous recombination; translesion DNA synthesis

Share and Cite

MDPI and ACS Style

Holíčková, A.; Roška, J.; Órásová, E.; Bruderová, V.; Palacka, P.; Jurkovičová, D.; Chovanec, M. Response of the Urothelial Carcinoma Cell Lines to Cisplatin. Int. J. Mol. Sci. 2022, 23, 12488. https://doi.org/10.3390/ijms232012488

AMA Style

Holíčková A, Roška J, Órásová E, Bruderová V, Palacka P, Jurkovičová D, Chovanec M. Response of the Urothelial Carcinoma Cell Lines to Cisplatin. International Journal of Molecular Sciences. 2022; 23(20):12488. https://doi.org/10.3390/ijms232012488

Chicago/Turabian Style

Holíčková, Andrea, Jan Roška, Eveline Órásová, Vladimíra Bruderová, Patrik Palacka, Dana Jurkovičová, and Miroslav Chovanec. 2022. "Response of the Urothelial Carcinoma Cell Lines to Cisplatin" International Journal of Molecular Sciences 23, no. 20: 12488. https://doi.org/10.3390/ijms232012488

APA Style

Holíčková, A., Roška, J., Órásová, E., Bruderová, V., Palacka, P., Jurkovičová, D., & Chovanec, M. (2022). Response of the Urothelial Carcinoma Cell Lines to Cisplatin. International Journal of Molecular Sciences, 23(20), 12488. https://doi.org/10.3390/ijms232012488

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop