Next Article in Journal
Metabolic Heterogeneity of Brain Tumor Cells of Proneural and Mesenchymal Origin
Next Article in Special Issue
Identification, Characterization and Expression Profiling of the RS Gene Family during the Withering Process of White Tea in the Tea Plant (Camellia sinensis) Reveal the Transcriptional Regulation of CsRS8
Previous Article in Journal
Prolonged Exposure to High Temperature Inhibits Shoot Primary and Root Secondary Growth in Panax ginseng
Previous Article in Special Issue
Heterologous Expression of MfWRKY7 of Resurrection Plant Myrothamnus flabellifolia Enhances Salt and Drought Tolerance in Arabidopsis
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 23
Issue 19
10.3390/ijms231911650
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Genome-Wide Analysis and Expression Profiling of Glutathione Reductase Gene Family in Oat (Avena sativa) Indicate Their Responses to Abiotic Stress during Seed Imbibition

by
Ming Sun
,
Shoujiang Sun
,
Zhicheng Jia
,
Wen Ma
,
Chunli Mao
,
Chengming Ou
,
Juan Wang
,
Han Zhang
,
Liu Hong
,
Manli Li
,
Shangang Jia
and
Peisheng Mao
*
Forage Seed Laboratory, College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(19), 11650; https://doi.org/10.3390/ijms231911650
Submission received: 11 August 2022 / Revised: 7 September 2022 / Accepted: 23 September 2022 / Published: 1 October 2022
(This article belongs to the Special Issue Regulatory Mechanism and Network of Abiotic Stress-Response in Plants)
Browse Figures
Versions Notes

Abstract

Abiotic stress disturbs plant cellular redox homeostasis, inhibiting seed germination and plant growth. This is a crucial limitation to crop yield. Glutathione reductase (GR) is an important component of the ascorbate-glutathione (AsA-GSH) cycle which is involved in multiple plant metabolic processes. In the present study, GRs in A. sativa (AsGRs) were selected to explore their molecular characterization, phylogenetic relationship, and RNA expression changes during seed imbibition under abiotic stress. Seven AsGR genes were identified and mapped on six chromosomes of A, C, and D subgenomes. Phylogenetic analysis and subcellular localization of AsGR proteins divided them into two sub-families, AsGR1 and AsGR2, which were predicted to be mainly located in cytoplasm, mitochondrion, and chloroplast. Cis-elements relevant to stress and hormone responses are distributed in promoter regions of AsGRs. Tissue-specific expression profiling showed that AsGR1 genes were highly expressed in roots, leaves, and seeds, while AsGR2 genes were highly expressed in leaves and seeds. Both AsGR1 and AsGR2 genes showed a decreasing-increasing expression trend during seed germination under non-stress conditions. In addition, their responses to drought, salt, cold, copper, H2O2, and ageing treatments were quite different during seed imbibition. Among the seven AsGR genes, AsGR1-A, AsGR1-C, AsGR2-A, and AsGR2-D responded more significantly, especially under drought, ageing, and H2O2 stress. This study has laid the ground for the functional characterization of GR and the improvement of oat stress tolerance and seed vigor.
Keywords: A. sativa; glutathione reductase; abiotic stress; seed imbibition; qPCR A. sativa; glutathione reductase; abiotic stress; seed imbibition; qPCR

Share and Cite

MDPI and ACS Style

Sun, M.; Sun, S.; Jia, Z.; Ma, W.; Mao, C.; Ou, C.; Wang, J.; Zhang, H.; Hong, L.; Li, M.; et al. Genome-Wide Analysis and Expression Profiling of Glutathione Reductase Gene Family in Oat (Avena sativa) Indicate Their Responses to Abiotic Stress during Seed Imbibition. Int. J. Mol. Sci. 2022, 23, 11650. https://doi.org/10.3390/ijms231911650

AMA Style

Sun M, Sun S, Jia Z, Ma W, Mao C, Ou C, Wang J, Zhang H, Hong L, Li M, et al. Genome-Wide Analysis and Expression Profiling of Glutathione Reductase Gene Family in Oat (Avena sativa) Indicate Their Responses to Abiotic Stress during Seed Imbibition. International Journal of Molecular Sciences. 2022; 23(19):11650. https://doi.org/10.3390/ijms231911650

Chicago/Turabian Style

Sun, Ming, Shoujiang Sun, Zhicheng Jia, Wen Ma, Chunli Mao, Chengming Ou, Juan Wang, Han Zhang, Liu Hong, Manli Li, and et al. 2022. "Genome-Wide Analysis and Expression Profiling of Glutathione Reductase Gene Family in Oat (Avena sativa) Indicate Their Responses to Abiotic Stress during Seed Imbibition" International Journal of Molecular Sciences 23, no. 19: 11650. https://doi.org/10.3390/ijms231911650

APA Style

Sun, M., Sun, S., Jia, Z., Ma, W., Mao, C., Ou, C., Wang, J., Zhang, H., Hong, L., Li, M., Jia, S., & Mao, P. (2022). Genome-Wide Analysis and Expression Profiling of Glutathione Reductase Gene Family in Oat (Avena sativa) Indicate Their Responses to Abiotic Stress during Seed Imbibition. International Journal of Molecular Sciences, 23(19), 11650. https://doi.org/10.3390/ijms231911650

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop