The authors wish to make a correction to this paper []. We inadvertently failed to mention an important previous work in the field. We would like to add the following sentence and a reference in the Discussion section on page seven, lines nine–ten, in-between these two sentences: “Pre-embedding immunogold labeling using 10-μm-thick frozen sections, which are obtained using a histo-cryostat, has been reported previously [13,14]” and “We compared the OCT-embedded histo-cryostat samples cut at −20 °C with the 2.3 M sucrose-embedded samples cut at −100 °C, and we found that the embedding media and cutting temperature are critical factors in reducing the ice crystal-induced cryo-damage”.
“Additionally, Kusumi et al. introduced CLEM using semithin (1 μm) cryosections at −65 to −75 °C in the backscattered electron (BSE)-mode scanning electron microscope [].”
Newly added reference as [15] in original paper, the order of some reference citations has been adjusted correspondingly.
The authors apologize for any inconvenience caused and state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.
References
- Kim, H.-L.; Riew, T.-R.; Park, J.; Lee, Y.; Kim, I.-B. Correlative Light and Electron Microscopy Using Frozen Section Obtained Using Cryo-Ultramicrotomy. Int. J. Mol. Sci 2021, 22, 4273. [Google Scholar] [CrossRef] [PubMed]
- Kusumi, S.; Koga, D.; Watanabe, T.; Shibata, M. Combination of a cryosectioning method and section scanning electron microscopy for immuno-scanning electron microscopy. Biomed. Res. 2018, 39, 21–25. [Google Scholar] [CrossRef] [PubMed] [Green Version]
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