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Article

Structural Determination and Genetic Identification of the O-Antigen from an Escherichia coli Strain, LL004, Representing a Novel Serogroup

by 1,†, 2,†, 2, 3,*, 2 and 1,*
1
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457, China
2
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Lihu Ave. 1800, Wuxi 214122, China
3
Wuxi School of Medicine, Jiangnan University, Lihu Ave. 1800, Wuxi 214122, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Alexander O. Chizhov
Int. J. Mol. Sci. 2021, 22(23), 12746; https://doi.org/10.3390/ijms222312746
Received: 22 September 2021 / Revised: 24 November 2021 / Accepted: 24 November 2021 / Published: 25 November 2021
The O-antigen is the outermost component of the lipopolysaccharide layer in Gram-negative bacteria, and the variation of O-antigen structure provides the basis for bacterial serological diversity. Here, we determined the O-antigen structure of an Escherichia coli strain, LL004, which is totally different from all of the E. coli serogroups. The tetrasaccharide repeating unit was determined as →4)-β-d-Galp-(1→3)-β-d-GlcpNAc6OAc(~70%)-(1→3)-β-d-GalpA-(1→3)-β-d-GalpNAc-(1→ with monosaccharide analysis and NMR spectra. We also characterized the O-antigen gene cluster of LL004, and sequence analysis showed that it correlated well with the O-antigen structure. Deletion and complementation testing further confirmed its role in O-antigen biosynthesis, and indicated that the O-antigen of LL004 is assembled via the Wzx/Wzy dependent pathway. Our findings, in combination, suggest that LL004 should represent a novel serogroup of E. coli. View Full-Text
Keywords: Escherichia coli; serogroup; O-antigen; structure; O-antigen gene cluster Escherichia coli; serogroup; O-antigen; structure; O-antigen gene cluster
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MDPI and ACS Style

Wang, J.; Xu, Y.; Qin, C.; Hu, J.; Yin, J.; Guo, X. Structural Determination and Genetic Identification of the O-Antigen from an Escherichia coli Strain, LL004, Representing a Novel Serogroup. Int. J. Mol. Sci. 2021, 22, 12746. https://doi.org/10.3390/ijms222312746

AMA Style

Wang J, Xu Y, Qin C, Hu J, Yin J, Guo X. Structural Determination and Genetic Identification of the O-Antigen from an Escherichia coli Strain, LL004, Representing a Novel Serogroup. International Journal of Molecular Sciences. 2021; 22(23):12746. https://doi.org/10.3390/ijms222312746

Chicago/Turabian Style

Wang, Jing, Yujuan Xu, Chunjun Qin, Jing Hu, Jian Yin, and Xi Guo. 2021. "Structural Determination and Genetic Identification of the O-Antigen from an Escherichia coli Strain, LL004, Representing a Novel Serogroup" International Journal of Molecular Sciences 22, no. 23: 12746. https://doi.org/10.3390/ijms222312746

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