Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts
Abstract
:1. Introduction
2. Results
2.1. LiCl Inhibits MDV Replication in CEF Cells
2.2. Time-Dependent Manner of LiCl Inhibition on MDV Replication
2.3. Inhibitory Effect of LiCl Occurs throughout Virus Replication
2.4. LiCl Affects the Infectivity of MDV Directly
2.5. LiCl Inhibits Expression of Interferon-Related Gene in MDV Infected Cells
3. Discussion
4. Materials and Methods
4.1. Viruses, Cells and Reagents
4.2. Virus Infection and LiCl Treatment
4.3. The Effect of Different Models of Drug Treatment
4.4. Evaluation of Virucidal Effect of LiCl on MDV
4.5. Virus Titrations and Plaque Area Determinations
4.6. Real-Time PCR for Viral Gene Expression
4.7. Western Blot Analysis
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Target Gene | Sequence | Product Size | Accession Number |
---|---|---|---|
Meq | F 5′-GTCCCCCCTCGATCTTTCTC-3′ R 5′-CGTCTGCTTCCTGCGTCTTC-3′ | 184 | NC-002229.3 |
gB | F 5′-ACCCCATTCGGTGGCTTTTC-3′ R 5′-GCGTCCAGTTGTCTGAGG-3′ | 122 | NC-002229.3 |
IRF7 | F 5′-CGTATCTTCCGCATCCCTTGG-3′ R 5′-TCGTCGTTGCACTTGGAGCG-3′ | 206 | NM-205372.1 |
IFN-β | F 5′-GCTCTCACCACCACCTTCTC-3′ R 5′-GCTTGCTTCTTGTCCTTGCT-3′ | 151 | NM-001024836.1 |
OASL | F 5′-GAGATAGAGAAGGAGTGGTG-3′ R 5′-GTAGACTGTGGTCTTGTTAC-3′ | 201 | NM_205041.2 |
18S | F 5′-TCAGATACCGTCGTAGTTCC-3′ R 5′-TTCCGTCAATTCCTTTAAGTT-3′ | 154 | AF173612 |
OVO | F 5′-CACTGCCACTGGGCTCTGT-3′ R 5′-GCAATGGCAATAAACCTCCAA-3′ | 71 | NM_205304.1 |
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He, H.; Qiao, D.; Zhang, L.; Yao, Y.; Shao, H.; Qin, A.; Qian, K. Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts. Int. J. Mol. Sci. 2021, 22, 12375. https://doi.org/10.3390/ijms222212375
He H, Qiao D, Zhang L, Yao Y, Shao H, Qin A, Qian K. Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts. International Journal of Molecular Sciences. 2021; 22(22):12375. https://doi.org/10.3390/ijms222212375
Chicago/Turabian StyleHe, Huifeng, Dandan Qiao, Lu Zhang, Yongxiu Yao, Hongxia Shao, Aijian Qin, and Kun Qian. 2021. "Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts" International Journal of Molecular Sciences 22, no. 22: 12375. https://doi.org/10.3390/ijms222212375