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Article

Metabolomic and Transcriptomic Profiling Provide Novel Insights into Fruit Ripening and Ripening Disorder Caused by 1-MCP Treatments in Papaya

Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center for Postharvest Technology of Horticultural Crops in South China, Ministry of Education, College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, China
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Int. J. Mol. Sci. 2021, 22(2), 916; https://doi.org/10.3390/ijms22020916
Received: 31 December 2020 / Revised: 15 January 2021 / Accepted: 15 January 2021 / Published: 18 January 2021
(This article belongs to the Section Molecular Plant Sciences)
Treatment with 1-methylcyclopropylene (1-MCP) is an effective technique to preserve fruits, but inappropriate treatment with 1-MCP causes a ripening disorder (rubbery texture) in papaya fruit. In this study, a combined metabolomic and transcriptomic analysis was conducted to reveal the possible mechanism of the ripening disorder caused by unsuitable 1-MCP in papaya. A total of 203 differential accumulated metabolites (DAMs) were identified in the metabolome analysis. Only 24 DAMs were identified in the control (CK) vs. the 1-MCP 2 h group, and they were primarily flavonoids. Ninety and 89 DAMs were identified in the CK vs. 1-MCP 16 h and 1-MCP 2 h vs. 1-MCP 16 h groups, respectively, indicating that long-term 1-MCP treatment severely altered the metabolites during fruit ripening. 1-MCP 16 h treatment severely reduced the number of metabolites, which primarily consisted of flavonoids, lipids, phenolic acids, alkaloids, and organic acids. An integrated analysis of RNA-Seq and metabolomics showed that various energy metabolites for the tricarboxylic acid cycle were reduced by long-term treatment with 1-MCP, and the glycolic acid cycle was the most significantly affected, as well as the phenylpropane pathway. These results provide valuable information for fruit quality control and new insight into the ripening disorder caused by unsuitable treatment with 1-MCP in papaya. View Full-Text
Keywords: metabolome; transcriptome analysis; 1-methylcyclopropylene; ripening disorder; phenylpropane pathway; fruit quality; papaya metabolome; transcriptome analysis; 1-methylcyclopropylene; ripening disorder; phenylpropane pathway; fruit quality; papaya
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MDPI and ACS Style

Zheng, S.; Hao, Y.; Fan, S.; Cai, J.; Chen, W.; Li, X.; Zhu, X. Metabolomic and Transcriptomic Profiling Provide Novel Insights into Fruit Ripening and Ripening Disorder Caused by 1-MCP Treatments in Papaya. Int. J. Mol. Sci. 2021, 22, 916. https://doi.org/10.3390/ijms22020916

AMA Style

Zheng S, Hao Y, Fan S, Cai J, Chen W, Li X, Zhu X. Metabolomic and Transcriptomic Profiling Provide Novel Insights into Fruit Ripening and Ripening Disorder Caused by 1-MCP Treatments in Papaya. International Journal of Molecular Sciences. 2021; 22(2):916. https://doi.org/10.3390/ijms22020916

Chicago/Turabian Style

Zheng, Senlin, Yanwei Hao, Silin Fan, Jiahui Cai, Weixin Chen, Xueping Li, and Xiaoyang Zhu. 2021. "Metabolomic and Transcriptomic Profiling Provide Novel Insights into Fruit Ripening and Ripening Disorder Caused by 1-MCP Treatments in Papaya" International Journal of Molecular Sciences 22, no. 2: 916. https://doi.org/10.3390/ijms22020916

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