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Article

Comparative Proteomic Analysis of the Mesenchymal Stem Cells Secretome from Adipose, Bone Marrow, Placenta and Wharton’s Jelly

1
Center for Theragnosis, Korea Institute of Science and Technology, Seoul 02792, Korea
2
KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul 02447, Korea
3
Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul 06351, Korea
4
R&D Center, ENCell Co., Ltd., Seoul 06351, Korea
5
Department of Physiology, School of Medicine, Kyung Hee University, Seoul 02447, Korea
6
Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul 01812, Korea
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Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea
8
Department of Plastic Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea
9
Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology, Seoul 02792, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Bogusław Machaliński
Int. J. Mol. Sci. 2021, 22(2), 845; https://doi.org/10.3390/ijms22020845
Received: 28 December 2020 / Revised: 12 January 2021 / Accepted: 13 January 2021 / Published: 15 January 2021
Mesenchymal stem cells (MSCs) have the potential to be a viable therapy against various diseases due to their paracrine effects, such as secretion of immunomodulatory, trophic and protective factors. These cells are known to be distributed within various organs and tissues. Although they possess the same characteristics, MSCs from different sources are believed to have different secretion potentials and patterns, which may influence their therapeutic effects in disease environments. We characterized the protein secretome of adipose (AD), bone marrow (BM), placenta (PL), and Wharton’s jelly (WJ)-derived human MSCs by using conditioned media and analyzing the secretome by mass spectrometry and follow-up bioinformatics. Each MSC secretome profile had distinct characteristics depending on the source. However, the functional analyses of the secretome from different sources showed that they share similar characteristics, such as cell migration and negative regulation of programmed cell death, even though differences in the composition of the secretome exist. This study shows that the secretome of fetal-derived MSCs, such as PL and WJ, had a more diverse composition than that of AD and BM-derived MSCs, and it was assumed that their therapeutic potential was greater because of these properties. View Full-Text
Keywords: mesenchymal stem cells; secretome; adipose; bone marrow; placenta; Wharton’s jelly; mass spectrometry mesenchymal stem cells; secretome; adipose; bone marrow; placenta; Wharton’s jelly; mass spectrometry
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MDPI and ACS Style

Shin, S.; Lee, J.; Kwon, Y.; Park, K.-S.; Jeong, J.-H.; Choi, S.-J.; Bang, S.I.; Chang, J.W.; Lee, C. Comparative Proteomic Analysis of the Mesenchymal Stem Cells Secretome from Adipose, Bone Marrow, Placenta and Wharton’s Jelly. Int. J. Mol. Sci. 2021, 22, 845. https://doi.org/10.3390/ijms22020845

AMA Style

Shin S, Lee J, Kwon Y, Park K-S, Jeong J-H, Choi S-J, Bang SI, Chang JW, Lee C. Comparative Proteomic Analysis of the Mesenchymal Stem Cells Secretome from Adipose, Bone Marrow, Placenta and Wharton’s Jelly. International Journal of Molecular Sciences. 2021; 22(2):845. https://doi.org/10.3390/ijms22020845

Chicago/Turabian Style

Shin, Sungho, Jeongmin Lee, Yumi Kwon, Kang-Sik Park, Jae-Hoon Jeong, Suk-Joo Choi, Sa I. Bang, Jong W. Chang, and Cheolju Lee. 2021. "Comparative Proteomic Analysis of the Mesenchymal Stem Cells Secretome from Adipose, Bone Marrow, Placenta and Wharton’s Jelly" International Journal of Molecular Sciences 22, no. 2: 845. https://doi.org/10.3390/ijms22020845

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