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Article

METTL8 mRNA Methyltransferase Enhances Cancer Cell Migration via Direct Binding to ARID1A

Department of Biochemistry, BK21 Plus and Research Institute for Veterinary Science, School of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
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Author to whom correspondence should be addressed.
Academic Editor: Peter Hamar
Int. J. Mol. Sci. 2021, 22(11), 5432; https://doi.org/10.3390/ijms22115432
Received: 12 April 2021 / Revised: 16 May 2021 / Accepted: 19 May 2021 / Published: 21 May 2021
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
The association of RNA modification in cancer has recently been highlighted. Methyltransferase like 8 (METTL8) is an enzyme and its role in mRNA m3C modification has barely been studied. In this study, we found that METTL8 expression was significantly up-regulated in canine mammary tumor and investigated its functional roles in the tumor process, including cancer cell proliferation and migration. METTL8 expression was up-regulated in most human breast cancer cell lines tested and decreased by Yin Yang 1 (YY1) transcription factor knockdown, suggesting that YY1 is a regulating transcription factor. The knockdown of METTL8 attenuated tumor cell growth and strongly blocked tumor cell migration. AT-rich interactive domain-containing protein 1A (ARID1A) was identified as a candidate mRNA by METTL8. ARID1A mRNA binds to METTL8 protein. ARID1A mRNA expression was not changed by METTL8 knockdown, but ARID1A protein level was significantly increased. Collectively, our study indicates that METTL8 up-regulated by YY1 in breast cancer plays an important role in cancer cell migration through the mRNA modification of ARID1A, resulting in the attenuation of its translation. View Full-Text
Keywords: mRNA modification; epigenetic regulation; breast cancer; ARID1A; cancer migration mRNA modification; epigenetic regulation; breast cancer; ARID1A; cancer migration
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MDPI and ACS Style

Lee, S.-A.; Lee, K.-H.; Kim, H.; Cho, J.-Y. METTL8 mRNA Methyltransferase Enhances Cancer Cell Migration via Direct Binding to ARID1A. Int. J. Mol. Sci. 2021, 22, 5432. https://doi.org/10.3390/ijms22115432

AMA Style

Lee S-A, Lee K-H, Kim H, Cho J-Y. METTL8 mRNA Methyltransferase Enhances Cancer Cell Migration via Direct Binding to ARID1A. International Journal of Molecular Sciences. 2021; 22(11):5432. https://doi.org/10.3390/ijms22115432

Chicago/Turabian Style

Lee, Shin-Ae, Kang-Hoon Lee, Huisu Kim, and Je-Yoel Cho. 2021. "METTL8 mRNA Methyltransferase Enhances Cancer Cell Migration via Direct Binding to ARID1A" International Journal of Molecular Sciences 22, no. 11: 5432. https://doi.org/10.3390/ijms22115432

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