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Quantification of 3D Brain Microangioarchitectures in an Animal Model of Krabbe Disease

1
Consiglio Nazionale delle Ricerche, Institute of Neuroscience, Via Vanvitelli 32, 20129 Milano, Italy
2
Unit of Experimental Oncology and Immunology, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(10), 2384; https://doi.org/10.3390/ijms20102384
Received: 18 April 2019 / Revised: 8 May 2019 / Accepted: 11 May 2019 / Published: 14 May 2019
(This article belongs to the Special Issue Recent Advances in Brain Vascular Diseases Management and Therapy)
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Abstract

We performed a three-dimensional (3D) analysis of the microvascular network of the cerebral cortex of twitcher mice (an authentic model of Krabbe disease) using a restricted set of indexes that are able to describe the arrangement of the microvascular tree in CD31-stained sections. We obtained a near-linear graphical “fingerprint” of the microangioarchitecture of wild-type and twitcher animals that describes the amounts, spatial dispersion, and spatial relationships of adjacent classes of caliber-filtered microvessels. We observed significant alterations of the microangioarchitecture of the cerebral cortex of twitcher mice, whereas no alterations occur in renal microvessels, which is keeping with the observation that kidney is an organ that is not affected by the disease. This approach may represent an important starting point for the study of the microvascular changes that occur in the central nervous system (CNS) under different physiopathological conditions. View Full-Text
Keywords: angioarchitecture; brain cortex; vessel quantification; angiogenesis; sphingolipidosis; neurodegeneration; image analysis; lysosomal storage diseases angioarchitecture; brain cortex; vessel quantification; angiogenesis; sphingolipidosis; neurodegeneration; image analysis; lysosomal storage diseases
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Righi, M.; Belleri, M.; Presta, M.; Giacomini, A. Quantification of 3D Brain Microangioarchitectures in an Animal Model of Krabbe Disease. Int. J. Mol. Sci. 2019, 20, 2384.

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