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Article

The miRNA Expression Profile of Experimental Autoimmune Encephalomyelitis Reveals Novel Potential Disease Biomarkers

1
Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
2
Institute of Genomic Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
3
Division of Rheumatology, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(12), 3990; https://doi.org/10.3390/ijms19123990
Received: 15 November 2018 / Accepted: 5 December 2018 / Published: 11 December 2018
(This article belongs to the Special Issue New Molecular Mechanisms in Multiple Sclerosis)
Multiple sclerosis (MS) is a debilitating autoimmune disease affecting over 2.3 million people worldwide, and it is characterized by inflammation and demyelination of nerve cells. The currently available biomarkers for the diagnosis and management of MS have inherent limitations, therefore, additional new biomarkers are needed. We studied the microRNA (miRNA) profile of splenocytes of mice having experimental autoimmune encephalomyelitis (EAE), a model of human MS. A miRNA-microarray analysis revealed increased expression of nine miRNAs (let-7e, miR-23b, miR-31, miR-99b, miR-125a, miR-146b, miR-155, miR-193b, and miR-221) following EAE development. Interestingly, serum levels of miR-99b, miR-125a, and miR-146b were significantly higher in EAE mice compared to normal mice. Bioinformatics analysis revealed the experimentally validated as well as predicted gene targets of specific miRNAs that are important for disease progression in MS. Specifically, we observed inverse correlation in the levels of miR-99b versus LIF, and between miR-125a versus BDNF and LIF. Our results suggest that above-mentioned miRNAs may play a crucial role in the pathogenesis of MS, and that miR-99b, miR-125a, and miR-146b in particular may serve as useful biomarkers for disease activity. View Full-Text
Keywords: experimental autoimmune encephalomyelitis; inflammation; Micro-RNA; miRNA; miR-99b; miR-125a; miR-146b; miR-193b; multiple sclerosis; myelin oligodendrocyte glycoprotein (MOG); MOG35–55 experimental autoimmune encephalomyelitis; inflammation; Micro-RNA; miRNA; miR-99b; miR-125a; miR-146b; miR-193b; multiple sclerosis; myelin oligodendrocyte glycoprotein (MOG); MOG35–55
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MDPI and ACS Style

Venkatesha, S.H.; Dudics, S.; Song, Y.; Mahurkar, A.; Moudgil, K.D. The miRNA Expression Profile of Experimental Autoimmune Encephalomyelitis Reveals Novel Potential Disease Biomarkers. Int. J. Mol. Sci. 2018, 19, 3990. https://doi.org/10.3390/ijms19123990

AMA Style

Venkatesha SH, Dudics S, Song Y, Mahurkar A, Moudgil KD. The miRNA Expression Profile of Experimental Autoimmune Encephalomyelitis Reveals Novel Potential Disease Biomarkers. International Journal of Molecular Sciences. 2018; 19(12):3990. https://doi.org/10.3390/ijms19123990

Chicago/Turabian Style

Venkatesha, Shivaprasad H., Steven Dudics, Yang Song, Anup Mahurkar, and Kamal D. Moudgil. 2018. "The miRNA Expression Profile of Experimental Autoimmune Encephalomyelitis Reveals Novel Potential Disease Biomarkers" International Journal of Molecular Sciences 19, no. 12: 3990. https://doi.org/10.3390/ijms19123990

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