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Int. J. Mol. Sci. 2018, 19(1), 44; https://doi.org/10.3390/ijms19010044

Light-Up RNA Aptamers and Their Cognate Fluorogens: From Their Development to Their Applications

Architecture et Réactivité de l’ARN, CNRS, Université de Strasbourg, UPR 9002, F-67000 Strasbourg, France
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Received: 29 November 2017 / Revised: 14 December 2017 / Accepted: 15 December 2017 / Published: 23 December 2017
(This article belongs to the Special Issue Aptamers)
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Abstract

An RNA-based fluorogenic module consists of a light-up RNA aptamer able to specifically interact with a fluorogen to form a fluorescent complex. Over the past decade, significant efforts have been devoted to the development of such modules, which now cover the whole visible spectrum, as well as to their engineering to serve in a wide range of applications. In this review, we summarize the different strategies used to develop each partner (the fluorogen and the light-up RNA aptamer) prior to giving an overview of their applications that range from live-cell RNA imaging to the set-up of high-throughput drug screening pipelines. We then conclude with a critical discussion on the current limitations of these modules and how combining in vitro selection with screening approaches may help develop even better molecules. View Full-Text
Keywords: light-up aptamer; fluorogen; fluorogenic dye; fluorescence; RNA; in vitro evolution; gene expression monitoring; live-cell imaging; biosensing light-up aptamer; fluorogen; fluorogenic dye; fluorescence; RNA; in vitro evolution; gene expression monitoring; live-cell imaging; biosensing
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Bouhedda, F.; Autour, A.; Ryckelynck, M. Light-Up RNA Aptamers and Their Cognate Fluorogens: From Their Development to Their Applications. Int. J. Mol. Sci. 2018, 19, 44.

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