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Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes

1
Department of Biochemistry and Developmental Biology, Faculty of Medicine, University of Helsinki, 00290 Helsinki, Finland
2
Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2017, 18(8), 1691; https://doi.org/10.3390/ijms18081691
Received: 17 July 2017 / Revised: 28 July 2017 / Accepted: 28 July 2017 / Published: 3 August 2017
(This article belongs to the Special Issue Optogenetic Approaches in Neuroscience)
Bacterial photoreceptors absorb light energy and transform it into intracellular signals that regulate metabolism. Bacterial phytochrome photoreceptors (BphPs), some cyanobacteriochromes (CBCRs) and allophycocyanins (APCs) possess the near-infrared (NIR) absorbance spectra that make them promising molecular templates to design NIR fluorescent proteins (FPs) and biosensors for studies in mammalian cells and whole animals. Here, we review structures, photochemical properties and molecular functions of several families of bacterial photoreceptors. We next analyze molecular evolution approaches to develop NIR FPs and biosensors. We then discuss phenotypes of current BphP-based NIR FPs and compare them with FPs derived from CBCRs and APCs. Lastly, we overview imaging applications of NIR FPs in live cells and in vivo. Our review provides guidelines for selection of existing NIR FPs, as well as engineering approaches to develop NIR FPs from the novel natural templates such as CBCRs. View Full-Text
Keywords: bacterial photoreceptor; near-infrared fluorescent protein; phytochrome; cyanobacteriochrome; allophycocyanin; tetrapyrrole bacterial photoreceptor; near-infrared fluorescent protein; phytochrome; cyanobacteriochrome; allophycocyanin; tetrapyrrole
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Oliinyk, O.S.; Chernov, K.G.; Verkhusha, V.V. Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes. Int. J. Mol. Sci. 2017, 18, 1691.

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