Next Article in Journal
Expression and Distribution Pattern of Aquaporin 4, 5 and 11 in Retinas of 15 Different Species
Previous Article in Journal
Environmental Factors Associated with Altered Gut Microbiota in Children with Eczema: A Systematic Review
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(7), 1143;

A Δ-9 Fatty Acid Desaturase Gene in the Microalga Myrmecia incisa Reisigl: Cloning and Functional Analysis

College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Authors to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 18 April 2016 / Revised: 5 July 2016 / Accepted: 9 July 2016 / Published: 16 July 2016
(This article belongs to the Section Molecular Plant Sciences)
Full-Text   |   PDF [2474 KB, uploaded 16 July 2016]   |  


The green alga Myrmecia incisa is one of the richest natural sources of arachidonic acid (ArA). To better understand the regulation of ArA biosynthesis in M. incisa, a novel gene putatively encoding the Δ9 fatty acid desaturase (FAD) was cloned and characterized for the first time. Rapid-amplification of cDNA ends (RACE) was employed to yield a full length cDNA designated as MiΔ9FAD, which is 2442 bp long in sequence. Comparing cDNA open reading frame (ORF) sequence to genomic sequence indicated that there are 8 introns interrupting the coding region. The deduced MiΔ9FAD protein is composed of 432 amino acids. It is soluble and localized in the chloroplast, as evidenced by the absence of transmembrane domains as well as the presence of a 61-amino acid chloroplast transit peptide. Multiple sequence alignment of amino acids revealed two conserved histidine-rich motifs, typical for Δ9 acyl-acyl carrier protein (ACP) desaturases. To determine the function of MiΔ9FAD, the gene was heterologously expressed in a Saccharomyces cerevisiae mutant strain with impaired desaturase activity. Results of GC-MS analysis indicated that MiΔ9FAD was able to restore the synthesis of monounsaturated fatty acids, generating palmitoleic acid and oleic acid through the addition of a double bond in the Δ9 position of palmitic acid and stearic acid, respectively. View Full-Text
Keywords: RACE; Acyl-ACP desaturase; Saccharomyces cerevisiae; fatty acid composition RACE; Acyl-ACP desaturase; Saccharomyces cerevisiae; fatty acid composition

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Xue, W.-B.; Liu, F.; Sun, Z.; Zhou, Z.-G. A Δ-9 Fatty Acid Desaturase Gene in the Microalga Myrmecia incisa Reisigl: Cloning and Functional Analysis. Int. J. Mol. Sci. 2016, 17, 1143.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top