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Int. J. Mol. Sci. 2015, 16(9), 21681-21694;

A New HPLC-MS Method for Measuring Maslinic Acid and Oleanolic Acid in HT29 and HepG2 Human Cancer Cells

Biochemistry and Molecular Biology Section, Department of Experimental Biology, University of Jaén, Campus Las Lagunillas, E-23071 Jaén, Spain
Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Campus Fuentenueva, E-18001 Granada, Spain
Author to whom correspondence should be addressed.
Academic Editor: Chang Y. Lee
Received: 30 July 2015 / Revised: 21 August 2015 / Accepted: 27 August 2015 / Published: 9 September 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in olive, have important properties for health and disease prevention. MA selectively inhibits cell proliferation of the HT29 human colon-cancer cell line by inducing selective apoptosis. For measuring the MA and OA concentration inside the cell and in the culture medium, a new HPLC-MS procedure has been developed. With this method, a determination of the amount of MA and OA incorporated into HT29 and HepG2 human cancer-cell lines incubated with different concentrations of MA corresponding to 50% growth inhibitory concentration (IC50), IC50/2, IC50/4, and IC50/8 has been made. The results demonstrate that this method is appropriate for determining the MA and OA concentration in different types of cultured cells and reveals the specific dynamics of entry of MA into HT29 and HepG2 cells. View Full-Text
Keywords: maslinic acid; oleanolic acid; HPLC-MS; HT29; HepG2 maslinic acid; oleanolic acid; HPLC-MS; HT29; HepG2

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Peragón, J.; Rufino-Palomares, E.E.; Muñoz-Espada, I.; Reyes-Zurita, F.J.; Lupiáñez, J.A. A New HPLC-MS Method for Measuring Maslinic Acid and Oleanolic Acid in HT29 and HepG2 Human Cancer Cells. Int. J. Mol. Sci. 2015, 16, 21681-21694.

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