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Int. J. Mol. Sci. 2015, 16(10), 24159-24173;

Effects of Pulsed Electric Fields (PEF) on Vitamin C and Its Antioxidant Properties

College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China
Centre for Food Research and Innovation, Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln 85084, New Zealand
Authors to whom correspondence should be addressed.
Academic Editor: Maurizio Battino
Received: 26 August 2015 / Revised: 29 September 2015 / Accepted: 7 October 2015 / Published: 13 October 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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In this study, pulsed electric fields (PEF) treatments and their effects on the structure of vitamin C (VIT-C) were estimated by fluorescence and Fourier transform infrared (FT-IR) spectroscopy, the relative content of VIT-C was measured by HPLC and the antioxidant properties of treated VIT-C by DPPH radical scavenging as well as reducing power tests. The fluorescence intensity of treated VIT-C increased slightly compared to the untreated VIT-C. Moreover, the effect of PEF on the structure of VIT-C was observed using the FT-IR spectra. These phenomena indicated that the PEF affected the conformation of VIT-C, which promoted the VIT-C isomer transformed enol-form into keto-form. In addition, the PEF treatments did not suffer the damage to VIT-C and could slow down the oxidation process in involving of experimental conditions by HPLC. The antioxidant properties of the treated VIT-C were enhanced, which was proved by radical scavenging and also the reducing power tests. View Full-Text
Keywords: vitamin C; PEF; fluorescence; FTIR; HPLC; antioxidant properties vitamin C; PEF; fluorescence; FTIR; HPLC; antioxidant properties

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Zhang, Z.-H.; Zeng, X.-A.; Brennan, C.S.; Brennan, M.; Han, Z.; Xiong, X.-Y. Effects of Pulsed Electric Fields (PEF) on Vitamin C and Its Antioxidant Properties. Int. J. Mol. Sci. 2015, 16, 24159-24173.

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