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Early Diagnosis of Irkut Virus Infection Using Magnetic Bead-Based Serum Peptide Profiling by MALDI-TOF MS in a Mouse Model

Military Veterinary Research Institute, Academy of Military Medical Sciences, 666 Liuying West Road, Jingyue Economic Development Zone, Changchun 130122, China
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Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2014, 15(4), 5193-5198; https://doi.org/10.3390/ijms15045193
Received: 16 January 2014 / Revised: 6 March 2014 / Accepted: 12 March 2014 / Published: 25 March 2014
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Early diagnosis is important for the prompt post-exposure prophylaxis of lyssavirus infections. To diagnose Irkut virus (IRKV) infection during incubation in mice, a novel method using magnetic bead-based serum peptide profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been established. For this test, serum peptides were concentrated by adsorption to and elution from the magnetic bead-based weak cation ion exchanger. Mass spectrograms obtained by MALDI-TOF MS were analyzed using ClinProTools bioinformatics software. Construction of the diagnostic model was performed using serum samples from mice infected with IRKV and rabies virus (RABV) BD06, Flury-LEP, and SRV9 (as controls). The method accurately diagnosed sera 2, 4 and 8 days after IRKV and RABV infections. The sensitivity, specificity, and total accuracy of diagnosis were 86.7%, 95.2%, and 92.9%, respectively. However, IRKV could not be differentiated from RABV 1 day after infection. The results of the present study indicate that serum peptide profiling by MALDI-TOF MS is a promising technique for the early clinical diagnosis of lyssavirus infections and needs to be further tested in humans and farm animals. View Full-Text
Keywords: lyssavirus; Irkut virus; rabies; early diagnosis; serum peptide profiling lyssavirus; Irkut virus; rabies; early diagnosis; serum peptide profiling
MDPI and ACS Style

Li, N.; Liu, Y.; Hao, Z.; Zhang, S.; Hu, R.; Li, J. Early Diagnosis of Irkut Virus Infection Using Magnetic Bead-Based Serum Peptide Profiling by MALDI-TOF MS in a Mouse Model. Int. J. Mol. Sci. 2014, 15, 5193-5198.

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