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Open AccessArticle

Analysis of Protein–Protein Interactions in MCF-7 and MDA-MB-231 Cell Lines Using Phthalic Acid Chemical

by Shih-Shin Liang 1,4,*, Tsu-Nai Wang 2,5 and Eing-Mei Tsai 3,4,*
1
Department of Biotechnology, College of Life Science, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
2
Department of Public Health, College of Health Science, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
3
Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
4
Center for Resources, Research and Development, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
5
Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2014, 15(11), 20770-20788; https://doi.org/10.3390/ijms151120770
Received: 12 September 2014 / Revised: 16 October 2014 / Accepted: 27 October 2014 / Published: 13 November 2014
(This article belongs to the Collection Proteins and Protein-Ligand Interactions)
Phthalates are a class of plasticizers that have been characterized as endocrine disrupters, and are associated with genital diseases, cardiotoxicity, hepatotoxicity, and nephrotoxicity in the GeneOntology gene/protein database. In this study, we synthesized phthalic acid chemical probes and demonstrated differing protein–protein interactions between MCF-7 cells and MDA-MB-231 breast cancer cell lines. Phthalic acid chemical probes were synthesized using silicon dioxide particle carriers, which were modified using the silanized linker 3-aminopropyl triethoxyslane (APTES). Incubation with cell lysates from breast cancer cell lines revealed interactions between phthalic acid and cellular proteins in MCF-7 and MDA-MB-231 cells. Subsequent proteomics analyses indicated 22 phthalic acid-binding proteins in both cell types, including heat shock cognate 71-kDa protein, ATP synthase subunit beta, and heat shock protein HSP 90-beta. In addition, 21 MCF-7-specific and 32 MDA-MB-231 specific phthalic acid-binding proteins were identified, including related proteasome proteins, heat shock 70-kDa protein, and NADPH dehydrogenase and ribosomal correlated proteins, ras-related proteins, and members of the heat shock protein family, respectively. View Full-Text
Keywords: phthalate; phthalic acid; protein–protein interaction; MCF-7; MDA-MB-231 phthalate; phthalic acid; protein–protein interaction; MCF-7; MDA-MB-231
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Liang, S.-S.; Wang, T.-N.; Tsai, E.-M. Analysis of Protein–Protein Interactions in MCF-7 and MDA-MB-231 Cell Lines Using Phthalic Acid Chemical. Int. J. Mol. Sci. 2014, 15, 20770-20788.

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