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Article

Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions

1
Department of Surgical Oncology, First Affiliated Hospital of China Medical University, Shenyang 110001, China
2
Department of Nephrology, First Affiliated Hospital of China Medical University, Shenyang 110001, China
3
Department of Histology and Embryology, Institute of Pathology and Pathophysiology, China Medical University, Shenyang 110001, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(3), 5998-6011; https://doi.org/10.3390/ijms14035998
Received: 18 January 2013 / Revised: 3 March 2013 / Accepted: 5 March 2013 / Published: 15 March 2013
(This article belongs to the Special Issue Molecular Research in Urology)
Given that podocalyxin (PCX) and nestin play important roles in podocyte morphogenesis and the maintenance of structural integrity, we examined whether the expression and localization of these two podocyte proteins were influenced in the early stage of various hemodynamic conditions. Mice kidney tissues were prepared by in vivo cryotechnique (IVCT). The distribution of glomeruli and podocyte proteins was visualized with DAB staining, confocal laser scanning microscopy and immunoelectron microscopy. The mRNA levels were examined by real-time quantitative PCR. The results showed the following: Under the normal condition, PCX stained intensely along glomerular epithelial cells, whereas nestin was clearly staining in the endothelial cells and appeared only weakly in the podocytes. Under the acute hypertensive and cardiac arrest conditions, PCX and nestin staining was not clear, with a disarranged distribution, but the colocalization of PCX and nestin was apparent under this condition. In addition, under the acute hypertensive and cardiac arrest conditions, the mRNA levels of PCX and nestin were significantly decreased. Collectively, the abnormal redistribution and decreased mRNA expressions of PCX and nestin are important molecular events at the early stage of podocyte injury during hemodynamic disorders. IVCT may have more advantages for morphological analysis when researching renal diseases. View Full-Text
Keywords: podocalyxin; nestin; acute hypertension; cardiac arrest; in vivo cryotechnique podocalyxin; nestin; acute hypertension; cardiac arrest; in vivo cryotechnique
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MDPI and ACS Style

Li, K.; Wang, J.; Yin, X.; Zhai, X.; Li, Z. Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions. Int. J. Mol. Sci. 2013, 14, 5998-6011. https://doi.org/10.3390/ijms14035998

AMA Style

Li K, Wang J, Yin X, Zhai X, Li Z. Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions. International Journal of Molecular Sciences. 2013; 14(3):5998-6011. https://doi.org/10.3390/ijms14035998

Chicago/Turabian Style

Li, Kai, Juan Wang, Xiaohui Yin, Xiaoyue Zhai, and Zilong Li. 2013. "Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions" International Journal of Molecular Sciences 14, no. 3: 5998-6011. https://doi.org/10.3390/ijms14035998

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