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HuR-Regulated mRNAs Associated with Nuclear hnRNP A1-RNP Complexes
Division of Biological Research and Biotechnology, Institute of Biology, Medicinal Chemistry and Biotechnology, National Hellenic Research Foundation, 48 Vas. Constantinou Avenue, Athens 11635, Greece
Laboratory of Biotechnology, School of Chemical Engineering, National Technical University of Athens, Athens 15780, Greece
* Authors to whom correspondence should be addressed.
Received: 29 July 2013; in revised form: 6 September 2013 / Accepted: 16 September 2013 / Published: 11 October 2013
Abstract: Post-transcriptional regulatory networks are dependent on the interplay of many RNA-binding proteins having a major role in mRNA processing events in mammals. We have been interested in the concerted action of the two RNA-binding proteins hnRNP A1 and HuR, both stable components of immunoselected hnRNP complexes and having a major nuclear localization. Specifically, we present here the application of the RNA-immunoprecipitation (RIP)-Chip technology to identify a population of nuclear transcripts associated with hnRNP A1-RNPs as isolated from the nuclear extract of either HuR WT or HuR-depleted (KO) mouse embryonic fibroblast (MEF) cells. The outcome of this analysis was a list of target genes regulated via HuR for their association (either increased or reduced) with the nuclear hnRNP A1-RNP complexes. Real time PCR analysis was applied to validate a selected number of nuclear mRNA transcripts, as well as to identify pre-spliced transcripts (in addition to their mature mRNA counterpart) within the isolated nuclear hnRNP A1-RNPs. The differentially enriched mRNAs were found to belong to GO categories relevant to biological processes anticipated for hnRNP A1 and HuR (such as transport, transcription, translation, apoptosis and cell cycle) indicating their concerted function in mRNA metabolism.
Keywords: RNA-binding proteins (RBPs); ribonucleoprotein (hnRNP) complexes; post-transcriptional regulation; mRNA processing; RNA-immunoprecipitation (RIP)-Chip technology; mouse embryonic fibroblasts (MEFs)
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Papadodima, O.; Chatziioannou, A.; Patrinou-Georgoula, M.; Kolisis, F.N.; Pletsa, V.; Guialis, A. HuR-Regulated mRNAs Associated with Nuclear hnRNP A1-RNP Complexes. Int. J. Mol. Sci. 2013, 14, 20256-20281.
Papadodima O, Chatziioannou A, Patrinou-Georgoula M, Kolisis FN, Pletsa V, Guialis A. HuR-Regulated mRNAs Associated with Nuclear hnRNP A1-RNP Complexes. International Journal of Molecular Sciences. 2013; 14(10):20256-20281.
Papadodima, Olga; Chatziioannou, Aristotelis; Patrinou-Georgoula, Meropi; Kolisis, Fragiskos N.; Pletsa, Vasiliki; Guialis, Apostolia. 2013. "HuR-Regulated mRNAs Associated with Nuclear hnRNP A1-RNP Complexes." Int. J. Mol. Sci. 14, no. 10: 20256-20281.