Polyphenol Content and Modulatory Activities of Some Tropical Dietary Plant Extracts on the Oxidant Activities of Neutrophils and Myeloperoxidase
Abstract
:1. Introduction
2. Results and Discussion
2.1. Results
2.1.1. Biochemical Investigations
2.1.1.1. Cell viability in Presence of the Plant Extracts Tested
2.1.1.2. Effects on the Total ROS Amount Produced by Neutrophils Activated with PMA (CL assay)
2.1.1.3. Effects on Total MPO Amount Released by Neutrophils Stimulated with PMA (MPO-ELISA)
2.1.1.4. Effects on the Nitration Activity of MPO Measured by Tyrosine nitration
2.1.1.5. Effects on the Nitration-Peroxidasic Activity of MPO Measured by SIEFED Technique
2.1.2. Phytochemical Analysis
2.2. Discussion
3. Experimental Section
3.1. Chemicals and Reagents
3.2. Vegetal Material
3.3. Preparation of the Plant Extracts for Biochemical Assays on Cell and Enzyme Models
3.4. Isolation of Equine Neutrophils
3.5. Preparation of the Neutrophil Activator and the Plant Extract Solution
3.6. Biochemical Investigations
3.6.1. Cell Viability Assay
3.6.2. Measurement of the Total ROS Produced by Neutrophils Activated with PMA- (CL Assay)
3.6.3. Measurement of Total MPO Released in the Extra-Cellular Milieu by Neutrophils Activated with PMA (MPO-ELISA Assay)
3.6.4. Measurement of the Nitration Activity of Myeloperoxidase in a Tyrosine Nitration Assay
3.6.5. Measurement of the Nitration-Peroxidasic Activity of Myeloperoxidase by SIEFED Technique
3.7. Phytochemical Analysis
3.7.1. Extract Preparation
3.7.2. Determination of Total Polyphenol in the Extracts
3.7.3. Determination of Tannins in the Extracts
3.7.4. HPLC-UV/DAD Determination of Total Flavonoid and Phenol Acid
3.8. Statistical Analysis
4. Conclusion
Acknowledgments
References
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IC50 (μg/mL) | ||||||
---|---|---|---|---|---|---|
Chemiluminescence | Tyrosine nitration | SIEFED technique | ||||
not heated | boiled | not heated | boiled | not heated | boiled | |
Abel | 10.40 ± 1.1 (0.901) | 10.77 ± 1.0 (0.942) | 10.06 ± 1.0 (0.962) | 10.92 ± 1.6 (0.913) | 13.57 ± 1.1 (0.834) | 14.71 ± 1.1 (0.824) |
Hib | 1.70 ± 0.8 (0.890) | 2.23 ± 1.1 (0.897) | 8.77 ± 1.1 (0.851) | 9.81 ± 1.1 (0.889) | 28.72 ± 1.2 (0.806) | 30.55 ± 1.6 (0.579) |
Man | 4.82 ± 1.0 (0.965) | 5.04 ± 1.0 (0.970) | 4.92 ± 1.0 (0.963) | 5.23 ± 1.0 (0.966) | 4.96 ± 1.0 (0.967) | 5.04 ± 1.0 (0.996) |
Pter | 1.43 ± 0.8 (0.907) | 1.89 ± 0.8 (0.688) | 1.10 ± 0.7 (0.827 | 1.14 ± 0.4 (0.940) | 1.50 ± 0.8 (0.917) | 1.28 ± 0.7 (0.921) |
Abelmoschus | Hibiscus | Manihot | Pteridium | |
---|---|---|---|---|
Total polyphenol a | 1,480 | 1,730 | N.D. | 6,740 |
Tannin b | 600 | 500 | N.D. | 5,550 |
Non-tannin | 880 | 1,230 | N.D. | 1,190 |
Total Flavonoid c | 425 | 775 | 1,381 | 448 |
Total phenolic acid d | N.D. | 975 | N.D. | 278 |
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Tsumbu, C.N.; Deby-Dupont, G.; Tits, M.; Angenot, L.; Frederich, M.; Kohnen, S.; Mouithys-Mickalad, A.; Serteyn, D.; Franck, T. Polyphenol Content and Modulatory Activities of Some Tropical Dietary Plant Extracts on the Oxidant Activities of Neutrophils and Myeloperoxidase. Int. J. Mol. Sci. 2012, 13, 628-650. https://doi.org/10.3390/ijms13010628
Tsumbu CN, Deby-Dupont G, Tits M, Angenot L, Frederich M, Kohnen S, Mouithys-Mickalad A, Serteyn D, Franck T. Polyphenol Content and Modulatory Activities of Some Tropical Dietary Plant Extracts on the Oxidant Activities of Neutrophils and Myeloperoxidase. International Journal of Molecular Sciences. 2012; 13(1):628-650. https://doi.org/10.3390/ijms13010628
Chicago/Turabian StyleTsumbu, Cesar N., Ginette Deby-Dupont, Monique Tits, Luc Angenot, Michel Frederich, Stephane Kohnen, Ange Mouithys-Mickalad, Didier Serteyn, and Thierry Franck. 2012. "Polyphenol Content and Modulatory Activities of Some Tropical Dietary Plant Extracts on the Oxidant Activities of Neutrophils and Myeloperoxidase" International Journal of Molecular Sciences 13, no. 1: 628-650. https://doi.org/10.3390/ijms13010628