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Open AccessArticle

Development and Validation of a Method for Determining the Quercetin-3-O-glucuronide and Ellagic Acid Content of Common Evening Primrose (Oenothera biennis) by HPLC-UVD

1
Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Deogyeong-daero 1732, Giheung-gu, Yongin 17104, Korea
2
Department of Integrated Biomedical and Life Science, Korea University, Seoul 02841, Korea
3
Dain Natural Co., Ltd., 130-33, Gasong-ro, Pungse-myeon, Dongnam-gu, Cheonan 31216, Korea
4
Skin-Biotechnology Center, Kyung Hee University, Gwanggyo-ro 147, Yeongtong-gu, Suwon 16229, Korea
5
Graduate School of Biotechnology, Kyung Hee University, Deogyeong-daero 1732, Giheung-gu, Yongin 17104, Korea
*
Authors to whom correspondence should be addressed.
Academic Editors: Monika Waksmundzka-Hajnos and Miroslaw Hawryl
Molecules 2021, 26(2), 267; https://doi.org/10.3390/molecules26020267
Received: 10 December 2020 / Revised: 27 December 2020 / Accepted: 2 January 2021 / Published: 7 January 2021
(This article belongs to the Special Issue Chromatographic Science of Natural Products II)
Toward the standardization of common evening primrose (Oenothera biennis) sprout extract (OBS-E), we aimed to obtain indicator compounds and use a validated method. HPLC-UVD allowed simultaneous quantification of the indicator compounds quercetin-3-O-glucuronide and ellagic acid. The method was validated in terms of specificity, linearity, precision, accuracy, and limit of detection/limit of quantification (LOD/LOQ). High specificity and linearity was demonstrated, with correlation coefficients of 1.0000 for quercetin-3-O-glucuronide and 0.9998 for ellagic acid. The LOD/LOQ values were 0.486/1.472 μg/mL for quercetin-3-O-glucuronide and 1.003/3.039 μg/mL for ellagic acid. Intra-day and inter-day variability tests produced relative standard deviation for each compound of <2%, a generally accepted precision criterion. High recovery rate were also obtained, indicating accuracy validation. The OBS-E prepared using various concentrations of ethanol were then analyzed. The 50% ethanol extract had highest content of quercetin-3-O-glucuronide, whereas the 70% ethanol extract possessed the lowest. However, the ellagic acid content was highest in the 70% ethanol extract and lowest in the 90% ethanol extract. Thus, quercetin-3-O-glucuronide and ellagic acid can be used industrially as indicator compounds for O. biennis sprout products, and our validated method can be used to establish indicator compounds for other natural products. View Full-Text
Keywords: Oenothera biennis; standardization; quercetin-3-O-glucuronide; ellagic acid; method validation Oenothera biennis; standardization; quercetin-3-O-glucuronide; ellagic acid; method validation
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MDPI and ACS Style

Kim, T.H.; Shin, H.Y.; Park, S.Y.; Kim, H.; Chung, D.K. Development and Validation of a Method for Determining the Quercetin-3-O-glucuronide and Ellagic Acid Content of Common Evening Primrose (Oenothera biennis) by HPLC-UVD. Molecules 2021, 26, 267. https://doi.org/10.3390/molecules26020267

AMA Style

Kim TH, Shin HY, Park SY, Kim H, Chung DK. Development and Validation of a Method for Determining the Quercetin-3-O-glucuronide and Ellagic Acid Content of Common Evening Primrose (Oenothera biennis) by HPLC-UVD. Molecules. 2021; 26(2):267. https://doi.org/10.3390/molecules26020267

Chicago/Turabian Style

Kim, Tae H.; Shin, Hyun Y.; Park, Soon Y.; Kim, Hoon; Chung, Dae K. 2021. "Development and Validation of a Method for Determining the Quercetin-3-O-glucuronide and Ellagic Acid Content of Common Evening Primrose (Oenothera biennis) by HPLC-UVD" Molecules 26, no. 2: 267. https://doi.org/10.3390/molecules26020267

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