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Molecules 2018, 23(8), 1925; https://doi.org/10.3390/molecules23081925

An Ultra-High Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Determination of 10 Alkaloids in Beagle Dog Plasma after the Oral Administration of the Corydalis yanhusuo W.T. Wang Extract and Yuanhuzhitong Tablets

1
Department of Pharmaceutical Analysis and Analytical Chemistry, College of Pharmacy, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin 150081, Heilongjiang, China
2
Department of Physiology, Basic Medical College of Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang, China
3
Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang, China
These authors contribute equally to this work.
*
Author to whom correspondence should be addressed.
Received: 10 July 2018 / Revised: 24 July 2018 / Accepted: 25 July 2018 / Published: 2 August 2018
(This article belongs to the Section Analytical Chemistry)
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Abstract

This study has developed a sensitive and simple ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry method for the simultaneous determination of corydaline, dehydrocorydaline, tetrahydropalmatine, protopine, palmatine, tetrahydroberberine, columbamine, berberine, coptisine and berberrubine in beagle dog plasma after the oral administration of the Corydalis yanhusuo W.T. Wang and Yuanhuzhitong tablets. Chromatographic separation was achieved on an Agilent Eclipse Plus C18 RRHD column (1.8 µm, 50 × 2.1 mm) using a gradient elution program with a mobile phase consisting of acetonitrile and water containing 0.1% formic acid at a flow rate of 0.3 mL/min. A tandem mass spectrometric detection was conducted by multiple reaction monitoring (MRM) mode via an electrospray ionization source in the positive mode. The calibration curves of all analytes showed good linear (r2 > 0.9800). The intra-day and inter-day precisions were less than 15% and the accuracies were within ±15%. The extraction recoveries conformed to the acceptable range. And there was no interference of endogenous substances in the sensitive assay method. All analytes were proven to be stable during sample storage and analysis procedures. The pharmacokinetic study indicated that the Yuanhuzhitong tablets could get a better absorption than Corydalis yanhusuo W.T. Wang. View Full-Text
Keywords: ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry; Corydalis yanhusuo W.T. Wang; Yuanhuzhitong tablets; pharmacokinetics ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry; Corydalis yanhusuo W.T. Wang; Yuanhuzhitong tablets; pharmacokinetics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Cui, B.; Yang, J.; Wang, Z.; Wu, C.; Dong, H.; Ren, Y.; Yang, C. An Ultra-High Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Determination of 10 Alkaloids in Beagle Dog Plasma after the Oral Administration of the Corydalis yanhusuo W.T. Wang Extract and Yuanhuzhitong Tablets. Molecules 2018, 23, 1925.

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