Kaempferol Attenuates ROS-Induced Hemolysis and the Molecular Mechanism of Its Induction of Apoptosis on Bladder Cancer

Wu, Ping; Meng, Xiaofeng; Zheng, Huade; Zeng, Qin; Chen, Tianfeng; Wang, Wen; Zhang, Xia; Su, Jianyu

doi:10.3390/molecules23102592

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Kaempferol Attenuates ROS-Induced Hemolysis and the Molecular Mechanism of Its Induction of Apoptosis on Bladder Cancer

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School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China

Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China

Department of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China

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Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA

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Department of Chemistry, Jinan University, Guangzhou 510632, China

Authors to whom correspondence should be addressed.

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These authors contributed equally to this work.

Molecules 2018, 23(10), 2592; https://doi.org/10.3390/molecules23102592

Received: 30 August 2018 / Revised: 5 October 2018 / Accepted: 8 October 2018 / Published: 10 October 2018

(This article belongs to the Special Issue Chemistry and Pharmacology of Modulators of Oxidative Stress 2018)

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Abstract

Bladder cancer has become the most common malignant urinary carcinoma. Studies have shown that significant antioxidant and bladder cancer-fighting properties of several plant-based diets like Psidium guajava, ginger and amomum, are associated with their high kaempferol content. In this paper, we evaluated the antioxidant and anticancer activities of kaempferol and its mechanism of induction to apoptosis on bladder cancer cells. Our findings demonstrated that kaempferol showed an obvious radical scavenging activity in erythrocytes damaged by oxygen. Kaempferol promoted antioxidant enzymes, inhibited ROS generation and lipid peroxidation and finally prevented the occurrence of hemolysis. Additionally, kaempferol exhibited a strong inhibitory effect on bladder cancer cells and high safety on normal bladder cells. At the molecular level, kaempferol suppressed EJ bladder cancer cell proliferation by inhibiting the function of phosphorylated AKT (p-AKT), CyclinD1, CDK4, Bid, Mcl-1 and Bcl-xL, and promoting p-BRCA1, p-ATM, p53, p21, p38, Bax and Bid expression, and finally triggering apoptosis and S phase arrest. We found that Kaempferol exhibited strong anti-oxidant activity on erythrocyte and inhibitory effects on the growth of cancerous bladder cells through inducing apoptosis and S phase arrest. These findings suggested that kaempferol might be regarded as a bioactive food ingredient to prevent oxidative damage and treat bladder cancer. View Full-Text

Keywords: kaempferol; antioxidant activity; anti-bladder cancer cell activity; apoptosis; p53 pathway

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

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MDPI and ACS Style

Wu, P.; Meng, X.; Zheng, H.; Zeng, Q.; Chen, T.; Wang, W.; Zhang, X.; Su, J. Kaempferol Attenuates ROS-Induced Hemolysis and the Molecular Mechanism of Its Induction of Apoptosis on Bladder Cancer. Molecules 2018, 23, 2592. https://doi.org/10.3390/molecules23102592

AMA Style

Wu P, Meng X, Zheng H, Zeng Q, Chen T, Wang W, Zhang X, Su J. Kaempferol Attenuates ROS-Induced Hemolysis and the Molecular Mechanism of Its Induction of Apoptosis on Bladder Cancer. Molecules. 2018; 23(10):2592. https://doi.org/10.3390/molecules23102592

Chicago/Turabian Style

Wu, Ping, Xiaofeng Meng, Huade Zheng, Qin Zeng, Tianfeng Chen, Wen Wang, Xia Zhang, and Jianyu Su. 2018. "Kaempferol Attenuates ROS-Induced Hemolysis and the Molecular Mechanism of Its Induction of Apoptosis on Bladder Cancer" Molecules 23, no. 10: 2592. https://doi.org/10.3390/molecules23102592

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Kaempferol Attenuates ROS-Induced Hemolysis and the Molecular Mechanism of Its Induction of Apoptosis on Bladder Cancer

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