Search Results (1,906)

Entomopathogenic fungi (EPF) are one of the most environmentally friendly ways to control a plethora of chewing insects such as T. pityocampa, G. mellonella, and A. grisella. Bioassay of EPF on these highly damaging pests is considered important in the face of climate change in order to research alternative solutions that are capable of limiting chemical control, the overuse of which increases insects’ resistance to chemical compounds. In this study, the insecticidal virulence of Metarhizium robertsii isolates, retrieved from forest ecosystems, was tested on second-instar larvae of T. pityocampa, G. mellonella, and A. grisella. Bioassays were carried out in the laboratory, where experimental larvae were sprayed with 2 mL of a six-conidial suspension from each isolate. Mortality was recorded for 144 h after exposure. Mean mortality, lethal concentrations, sporulation percentage, and sporulation time were estimated for each isolate. Metarhizium isolates resulted in the highest mortality (89.2% for G. mellonella and 90.2% for A. grisella). Based on the LC₅₀ estimates determined by the concentration–mortality relationships for the tested fungal isolates, we demonstrated significant virulence on larvae of G. mellonella, A. grisella, and T. pityocampa. Our results indicate that entomopathogenic fungi have the potential to become a very useful tool in reducing chemical applications. Full article

Ensuring bacterial safety of blood transfusions remains a critical focus in medicine. We investigated a novel pathogen reduction technology utilizing nylon functionalized with synthetic dyes (nylon affinity networks) to capture and remove bacteria from plasma. In the initial screening process, we spiked phosphate buffer solution (PBS) and human plasma (1 mL each) with 10 or 100 colony forming units (cfu) of either Escherichia coli or Staphylococcus epidermidis, exposed the suspensions to affinity networks and assessed the extent of bacterial reduction using agar plate cultures as the assay output. Nineteen synthetic dyes were tested. Among these, Alcian Blue exhibited the best performance with both bacterial strains in both PBS and plasma. Next, bacterial suspensions of approximately 1 and 2 cfu/mL in 10 and 50 mL, respectively, were treated with Alcian Blue affinity networks in three sequential capture steps. This procedure resulted in complete bacterial depletion, as demonstrated by the lack of bacterial growth in the remaining fraction. The viability of the captured bacteria was confirmed by plating the post-treatment affinity networks on agar. Alcian Blue affinity networks captured and sequestered a few plasma proteins identified by liquid chromatography tandem mass spectrometry. These findings support the potential applicability of nylon affinity networks to enhance transfusion safety, although additional investigations are needed. Full article

With the application of tracked mobile robots in detection and rescue, how to improve their stability and trafficability has become the research focus. In order to improve the driving ability and trafficability of tracked mobile robots in rugged terrain, this paper proposes a new type of tracked mobile robot using passive suspension. By adding a connecting rod differential mechanism between the left and right track mechanisms, the contact stability between the track and terrain is enhanced. The kinematics model and attitude relationship of the suspension are analyzed and established, and the rationality of the passive suspension scheme is verified by dynamic simulation. The simulation results show that the tracked robot with passive suspension shows good obstacle surmounting performance, but there will be a heading deflection problem. Therefore, a track drive speed of the driving state compensation control is proposed based on the driving scene, which can effectively solve the problem of slip and heading deflection. Through the field test of the robot prototype, the effectiveness of the suspension scheme and control system is verified, which provides a useful reference for the scheme design and performance improvement of the tracked mobile robot in complex field scenes. Full article

In response to the need for separation and utilization of residual film mixtures after transformation of protaetia brevitarsis larvae, a pneumatic drum-sieve-type separator for transforming mixtures of protaetia brevitarsis larvae was designed. First, the suspension velocity of each component was determined by the suspension speed test. Secondly, the separation process of residual film, larvae, and insect sand was formulated on the basis of biological activities, shape differences, and aerodynamic response characteristics. Eventually, the main structural parameters and working parameters of the machine were determined. In order to optimize the separation effect, a single-factor experiment and a quadratic regression response surface experiment containing three factors and three levels were carried out, and the corresponding regression model was established. The experimental results showed that the effects of the air speed at the inlet, inclination angle of the sieve cylinder, and rotational speed of the sieve cylinder on the impurity rate of the residual film decreased in that order, and that the effects of the rotational speed of the sieve cylinder, inclination angle of the sieve cylinder, and air speed at the inlet on the inactivation rate of the larvae decreased in that order. Through parameter optimization, a better combination of working parameters was obtained: the rotational speed of the sieve cylinder was 24 r/min, the inclination angle of the sieve cylinder was −0.43°, and the air speed at the inlet was 5.32 m/s. The average values of residual film impurity rate and larval inactivation rate obtained from the material sieving test under these parameters were 8.74% and 3.18%, with the relative errors of the theoretically optimized values being less than 5%. The results of the study can provide a reference for the resource utilization of residual film and impurity mixtures and the development of equipment for the living body separation of protaetia brevitarsis. Full article

This paper proposes a novel suspended seismic structure system called Base-suspended Pendulum Isolation (BSPI) structure. The BSPI structure can isolate seismic action and reduce structural seismic response by hanging the structure with hanger rods set at the base. The viscous dampers are installed in the isolation layer to dissipate earthquake energy and control the displacement. Firstly, the configuration of suspension isolation layer and mechanical model of the BSPI structure are described. Then, an equivalent scaled BSPI structure physical model was tested on the shaking table. The test results demonstrate that the BSPI structure has a good isolation effect under earthquakes, and the viscous dampers had an obvious control effect on the structure’s displacement and acceleration response. Finally, numerical simulation of the tests was carried out. The accuracy of the numerical models are confirmed by the good agreement between the simulation and test results. The numerical models for the BSPI structure and conventional reinforced concrete (RC) frame structure are built and analyzed using the commercial software ABAQUS. Research results indicate that the lateral stiffness of the BSPI structure is reduced greatly by installing the suspension layer, and the acceleration response of BSPI structure is significantly reduced under rare earthquakes, which is only 1/2 of that of the RC frame. The inter-story displacement of the BSPI structure is less than 1/100, which meets the seismic fortification goal and is reduced to 50% of that of the BSPI structure without damper under rare earthquakes. Full article

Background: The effect of antibiotics can be severely affected by external factors. Combining the oxidative impact of photodynamic therapy with antibiotics is largely unexplored, which may result in positive results with great impact on clinical applications. In particular, that can be relevant in the case of antibiotic resistance. Objectives: In this study, we examined the effects of aPDT using the photosensitizers (PSs), methylene blue (MB) or Photodithazine (PDZ), both alone and in combination with the antibiotics ciprofloxacin (CIP), gentamicin (GEN), and ceftriaxone (CEF), against the Gram-negative bacterium Klebsiella pneumoniae. Methods: A standard suspension of K. pneumoniae was subjected to PDT with varying doses of MB and PDZ solutions, using a 75 mW/cm² LED emitting at 660 nm with an energy of 15 J/cm². The MICs of CIP, GEN, and CEF were determined using the broth dilution method. We also tested the photosensitizers MB or PDZ as potentiating agents for synergistic combinations with antibiotics CIP, GEN, and CEF against K. pneumoniae. Results: The results showed that MB was more effective in inhibiting survival and killing K. pneumoniae compared to PDZ. The tested antibiotics CIP, GEN, and CEF suppressed bacterial growth (as shown by reduced MIC values) and effectively killed K. pneumoniae (reduced Log CFU/mL). While antibiotic treatment or aPDT alone showed a moderate effect (1 Log₁₀ to 2 Log₁₀ CFU reduction) on killing K. pneumoniae, the combination therapy significantly increased bacterial death, resulting in a ≥3 Log₁₀ to 6 Log₁₀ CFU reduction. Conclusions: Our study indicates that pre-treating bacteria with PDT makes them more susceptible to antibiotics and could serve as an alternative for treating local infections caused by resistant bacteria or even reduce the required antibiotic dosage. This work explores numerous possible combinations of PDT and antibiotics, emphasizing their interdependence in controlling infections and the unique properties each PS-antibiotic combination offers. Clinical application for the combination is a promising reality since both are individually already adopted in clinical use. Full article

Background: Some neonates are assessed for the risk of atypical psychomotor development at birth and are referred for reflex locomotion therapy using the Vojta method. Aim: The aim of this study was to analyze the relationships between spontaneous motor activity (SMA), ideal movement patterns (IMPs), central coordination disorders (CCDs), vital signs at birth, involuntary reflexes, and postural asymmetry in infants. Methods: This study involved 90 female and 107 male subjects in the age interval of 1–16 months (4.15 ± 2.18). Their psychomotor development was assessed using the Vojta method. Age-appropriate involuntary reflexes were evaluated, and both parameters were correlated with perinatal risk factors. Results: Males scored significantly higher than females (difference of −0.7, p = 0.022) in the SMA test. In both genders, SMA (p < 0.001 in both genders) and IMP scores improved significantly with age. In male infants, higher CCD scores were associated with significantly lower SMA and IMP scores (p = 0.017 and p < 0.001, respectively). Significantly higher CCD scores were noted in female subjects with the Moro reflex and postural asymmetry (p = 0.003 and p = 0.002, respectively). In males, the Moro reflex was significantly correlated with the Vojta reaction (p = 0.012) and the Collis vertical suspension reflex (p < 0.001). Conclusions: Vital signs at birth, including birth weight, Apgar score, and type of delivery, can predict motor development disorders but do not clearly differentiate infants that require neurodevelopmental therapy. Full article

Depression in cancer survivors is commonly treated with serotonin and norepinephrine reuptake inhibitors (SNRIs), such as venlafaxine. These drugs alleviate depressive symptoms by inhibiting the reuptake of serotonin and norepinephrine. However, a novel approach has emerged with the development of trans-2-phenylcyclopropylamine (PCPA)–drug conjugates that inhibit lysine-specific demethylase 1 (LSD1), which is a biomarker and molecular target for cancer therapy. LSD1 inhibition can effectively suppress cancer cell proliferation. Nuc01 is a novel PCPA–drug conjugate designed as a prodrug of venlafaxine. In vivo studies showed that Nuc01 dose-dependently reduced immobility time in the tail suspension test in mice, outperforming desmethylvenlafaxine. This suggests that Nuc01 may act as a potent triple reuptake inhibitor, potentially offering enhanced efficacy in the treatment of depression. Additionally, in vitro studies demonstrated that Nuc01 effectively occupies the PCPA binding site within LSD1 (IC₅₀ = 530 nm) and inhibits the proliferation of MDA-MB-231 cancer cells (IC₅₀ = 1130 nm). These findings suggest that Nuc01 may function as an LSD1 inhibitor with potential anticancer properties. Collectively, the data indicate that Nuc01 appears to exhibit dual functional characteristics: acting as a triple reuptake inhibitor potentially applicable for depression treatment and as an LSD1 inhibitor demonstrating anticancer potential. Full article

Nepafenac is an anti-inflammatory drug used in ophthalmology, marketed as a suspension due to its low aqueous solubility. A solution formulation could provide better bioavailability than suspension and facilitate single unit doses, avoiding the use of preservatives which are required to maintain sterility in multidose packaging. In this study, solubilization of nepafenac was achieved in the presence of randomly methylated β-cyclodextrin (RAMEB) and the actual complexation was assessed by NMR and phase-solubility studies. It was also found that the addition of hydrophilic polymers plays an important role in allowing increased solubilization of nepafenac at the same cyclodextrin concentration. Compared to complexes of nepafenac with other cyclodextrins, only 5% RAMEB was sufficient to solubilize 0.3% (w/v) nepafenac, enabling for the first time the development of an ophthalmic solution that proved chemically and physically stable for 12 months at 25 °C. The formulated solutions of nepafenac were tested for cytotoxicity on human corneal epithelial cells (HCE-2) and the results suggest their potential as a valuable and safe alternative to the commercially available 0.3% (w/v) suspension of the drug. Full article

Persea americana (avocado) is a healthy fruit, rich in unsaturated fatty acids, various minerals, and vitamins. As avocado cultivation continues to expand globally, its development is increasingly constrained by concomitant diseases, among which fruit rot and anthracnose have emerged as significant threats to fruit quality. Menglian in Yunnan Province is the largest avocado production area in China. In November 2024, fruit rot was observed on avocado fruits in Yunnan, China, characterized by reddish-brown discoloration, premature ripening, softening, and pericarp decay, with a field infection rate of 22%. Concurrently, anthracnose was detected in avocado fruits, presenting as small dark brown spots that developed into irregular rust-colored lesions, followed by dry rot depressions, ultimately leading to soft rot, peeling, or hardened dry rot, with a field infection rate of 15%. Infected fruit samples were collected, and fungal strains were isolated, purified, and inoculated via spore suspension, followed by re-isolation. The strains were conclusively identified as Diaporthe phaseolorum (SWFU20, SWFU21) and Colletotrichum fructicola (SWFU12, SWFU13) through an integrated approach combining DNA extraction, polymerase chain reaction (PCR), sequencing, phylogenetic reconstruction, and morphological characterization. This is the first report of D. phaseolorum causing fruit rot and C. fructicola causing anthracnose on avocado in China. In future research, we will test methods for the control of D. phaseolorum and C. fructicola. The identification of these pathogens provides a foundation for future disease management research, supporting the sustainable development of the avocado industry. Full article

Concrete is the most important construction material, and improving its durability properties is a topic in constant development owing to the economic costs that the degradation of concrete implies. Different nanoparticles have been reported to improve concrete durability, although the positive results are not a generality. Among these nanomaterials, graphene oxide stands out as an option for improving concrete properties, such as its compressive strength, which could increase the useful life of concrete infrastructure. This study addresses the effects of graphene oxide on the durability properties of concrete, with the aim of obtaining data on the viability of graphene oxide as an additive in concrete. The incorporation of graphene oxide into concrete was carried out through graphene oxide suspensions that were incorporated into concrete mixtures with a high water/cement ratio. The characterization of concrete was done using non-destructive testing such as ultrasonic pulse velocity, electrical resistivity, porosity, capillary absorption, chloride ion permeability, and other characterization methods such as compressive strength, XPS, SEM, and EDS. Together, these tests provided an overview of the concrete durability properties that are improved, affected, or unchanged by the presence of graphene oxide. In this study, a chemical analysis was also carried out on concrete modified with graphene oxide. The results show that graphene oxide improves the compressive strength of concrete, but the effect on durability properties is negligible; however, there are indications that, in combination with other additives, improvements can be achieved, so it is advisable to continue with these studies. Full article

In response to the challenges associated with weld treatment during the on-site corrosion protection of hydraulic steel gates, this paper proposes a method utilizing a magnetic adsorption climbing robot to perform corrosion protection operations. Firstly, a magnetic adsorption climbing robot with a multi-wheel independent drive configuration is proposed as a mobile platform. The robot body consists of six joint modules, with the two middle joints featuring adjustable suspension. The joints are connected in series via an EtherCAT bus communication system. Secondly, the kinematic model of the climbing robot is analyzed and a PID trajectory tracking control method is designed, based on the kinematic model and trajectory deviation information collected by the vision system. Subsequently, the proposed kinematic model and trajectory tracking control method are validated through Python3 simulation and actual operation tests on a curved trajectory, demonstrating the rationality of the designed PID controller and control parameters. Finally, an intelligent software system for weld defect detection based on computer vision is developed. This system is demonstrated to conduct defect detection on images of the current weld position using a trained model. Full article

The design of the suspension system affects handling and stability, vibrations of the steered wheels, vehicle ride comfort, and tyre tread wear. One of the most important vibration parameters is acceleration; high acceleration values can have an adverse effect on both the driver and passengers, as well as on the components of the vehicle’s suspension and handling. This paper presents the results of the effects of acceleration on the components of a front-independent MacPherson suspension system. Data on the accelerations were obtained from theoretical and experimental studies. A simulation study was conducted, taking into account the elastic and damping characteristics of the elastic components. The experimental study was conducted under laboratory conditions by using a suspension tester, BEISSBARTH, and a measuring system developed with LabVIEW 2021 SP1 and MATLAB R2022b software. The experiments were conducted with different tyre pressures and by using bushings made from different materials. The experimental tests were conducted with two rubber bushings within the mounting of the arm, as well as a rubber bushing and a polyurethane bushing. The experimental results were compared and analyzed. Two theoretical models were considered: one is a mathematical model, and the other is a simulation model which uses the finite element method. Numerical dynamic analysis of the suspension was performed using the SolidWorks 2023. Full article

Plant growth-promoting rhizobacteria (PGPR) are eco-friendly and sustainable options for agrochemicals, particularly for enhancing crop productivity under stress conditions. The present research aims to isolate and characterize native PGPR from tomato rhizospheric soil and to evaluate their effectiveness as a dose-dependent response to enhance the growth of tomato seedlings. Out of 112 isolates, 10 bacterial strains were selected based on key PGPR traits, including indole-3-acetic acid (IAA), ammonia production, hydrogen cyanide (HCN), exopolysaccharide (EPS) synthesis, hydrolytic enzyme activity, potassium solubilization, antifungal activity against Fusarium oxysporum, and tolerance to pH and heat stress. Molecular identification via 16S rRNA gene sequencing confirmed that these isolates belong to the genera Serratia and Enterobacter. S. marcescens So-1 and Enterobacter sp. So-12 produced the highest levels of IAA (2.6–24.1 µg/mL). In vitro tomato seed germination tests using bacterial suspensions at three concentrations (10⁶, 10⁷, and 10⁸ CFU/mL) showed dose-dependent improvements, with T1 increasing germination up to 108.3% compared to the control. In polyhouse trials using cocopeat formulations, seedling growth improved noticeably. T2 increased the root length (28.3 ± 2.98 cm) by over 1560%, and the shoot length (35.7 ± 0.57 cm) increased by 55% against the control, whose root length is 1.7 ± 0.47. The chlorophyll amount of the treated leaves further showed significant results over the control. Collectively, these findings suggest that using native PGPR in a dose-dependent way can help tomato seedlings grow better and promote more sustainable crop production. Full article

Background/Objectives: Urolithin A (UA), a gut-derived metabolite of ellagitannins or ellagic acid, has recently gained attention for its potential benefits to brain health. The present research aimed to assess the antidepressant-like properties of UA in both in vitro and in vivo models and explored the molecular mechanisms underlying these effects. Methods: We investigated the antidepressant effects and mechanisms of UA in a model of corticosterone-induced damage to PC12 cells and in a model of chronic socially frustrating stress. Results: Our results demonstrate that UA treatment (5 and 10 μM) significantly alleviated cellular damage and inflammation in corticosterone (CORT)-treated PC12 cells. Furthermore, UA administration (50 and 100 mg/kg) significantly reduced immobility time in the mouse tail suspension test (TST) and forced swim test (FST), indicating its antidepressant-like activity. Additionally, treatment with UA led to the activation of the cAMP response element-binding protein (CREB)/brain-derived neurotrophic factor (BDNF) signaling cascade and triggered the activation of adenosine monophosphate-activated protein kinase (AMPK) during these processes. Importantly, pretreatment with AMPK-specific inhibitor Compound C abolished UA’s cytoprotective effects in PC12 cells, as well as its behavioral efficacy in the FST and TST, and its neurotrophic effects, highlighting the critical role of AMPK activation in mediating these effects. Furthermore, in the chronic social defeat stress (CSDS) mouse model, UA treatment (50 and 100 mg/kg) significantly alleviated depression-like behaviors, including reduced sucrose preference in the sucrose preference test, increased social avoidance behavior in the social interaction test, and anxiety-like behaviors, including diminished exploration, in the elevated plus maze test, suggesting the antidepressant-like and anxiolytic-like activities of UA. Moreover, UA treatment reversed elevated serum stress hormone levels, hippocampal inflammation, and the decreased AMPK/CREB/BDNF signaling pathway in the hippocampus of CSDS mice. Conclusions: Together, these results provide compelling evidence for UA as a viable dietary supplement or therapeutic option for managing depression. Full article