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Communication

3 Citations

7,259 Views

10 Pages

Antibody Light Chains: Key to Increased Monoclonal Antibody Yields in Expi293 Cells?

Siqi Gong,
Seijal Gautam,
Joshua D. Coneglio,
Hanna B. Scinto and
Ruth M. Ruprecht

Antibodies2022, 11(2), 37;https://doi.org/10.3390/antib11020037

18 May 2022

When constructing isogenic recombinant IgM–IgG pairs, we discovered that μ heavy chains strongly prefer partnering with λ light chains for optimal IgM expression in transiently cotransfected Expi293 cells. When μ chains were paired...

(This article belongs to the Special Issue Monoclonal Antibody-Directed Therapy Series II)

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Review

20 Citations

6,097 Views

14 Pages

Mucosal Antibodies: Defending Epithelial Barriers against HIV-1 Invasion

Ruth M. Ruprecht,
Bishal Marasini and
Rajesh Thippeshappa

Vaccines2019, 7(4), 194;https://doi.org/10.3390/vaccines7040194

23 November 2019

The power of mucosal anti-HIV-1 envelope immunoglobulins (Igs) to block virus transmission is underappreciated. We used passive immunization, a classical tool to unequivocally prove whether antibodies are protective. We mucosally instilled recombinan...

(This article belongs to the Special Issue Advances in Antibody-based HIV-1 Vaccine Development)

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