error_outline You can access the new MDPI.com website here. Explore and share your feedback with us.
 
 
Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Article Types

Countries / Regions

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Search Results (11,630)

Search Parameters:
Keywords = product design process

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
24 pages, 999 KB  
Article
From Linear to Circular: Barriers to Sustainable Transition in the Saudi Banking Sector
by Aroop Mukherjee and Luisa Pinto
Sustainability 2026, 18(2), 673; https://doi.org/10.3390/su18020673 (registering DOI) - 8 Jan 2026
Abstract
This study investigates the barriers in adopting the Circular Economy (CE) in Saudi Banking under Vision 2030 and using the Resource-Based View and stakeholder theory. This study examined how customer engagement, process innovation, and dynamic capabilities limit the implementation of CE. A quantitative, [...] Read more.
This study investigates the barriers in adopting the Circular Economy (CE) in Saudi Banking under Vision 2030 and using the Resource-Based View and stakeholder theory. This study examined how customer engagement, process innovation, and dynamic capabilities limit the implementation of CE. A quantitative, cross-sectional survey collected 418 responses from bank employees in Riyadh and was collected from January to March 2024. A 29-item Likert scale was analyzed with SmartPLS 4; measurement quality was strong, and confirmatory factor analysis confirmed construct validity. Results highlight the main barriers as customer resistance regulatory constraints and lack of adequate employee training. The construct is highly interconnected (r = 0.758), showing that improvements in customer engagement and process innovation strengthen dynamic capabilities. The study provides practical guidance for banks and policymakers on designing circular finance products, targeted training, and supportive regulations to accelerate the CE transition and achieve measurable sustainability outcomes in financial sectors, aligning with SDG 3, good health and well-being, and SDG 7, affordable and clean energy. Full article
(This article belongs to the Special Issue Business Circular Economy and Sustainability)
19 pages, 1218 KB  
Article
Analysis of Cutting Forces Response to Machining Parameters Under Dry and Wet Machining Conditions in X5CrNi18-10 Turning
by Csaba Felhő, Tanuj Namboodri and Daynier Rolando Delgado Sobrino
Eng 2026, 7(1), 33; https://doi.org/10.3390/eng7010033 - 8 Jan 2026
Abstract
The shift toward digital and smart manufacturing requires an accurate prediction of cutting behavior, such as cutting forces. Controlling cutting forces in machining is important for maintaining product quality, particularly in steels such as X5CrNi18-10. This steel has high toughness, which resists cutting, [...] Read more.
The shift toward digital and smart manufacturing requires an accurate prediction of cutting behavior, such as cutting forces. Controlling cutting forces in machining is important for maintaining product quality, particularly in steels such as X5CrNi18-10. This steel has high toughness, which resists cutting, thereby increasing overall cutting forces. Proper selection of machining parameters and conditions can help reduce cutting forces during machining. Several studies have been dedicated to understanding the influence of cutting parameters on cutting forces. However, limited attention is given to the influence of the cutting conditions on cutting forces. The primary objective of this study is to understand the behavior of cutting forces in chromium-nickel alloy steel by varying machining parameters, specifically cutting conditions (dry and wet), using a full factorial (31 × 22) design of experiments (DoE). The secondary objective is to develop a multilinear regression model to predict cutting forces. The root mean square (RMS) values of the cutting force components were calculated from the acquired data and analyzed using OriginPro 2025b. In addition, this study analyzes the effects of cutting parameters and cutting forces on root mean square (RMS) surface roughness (Rq) to understand their impact on quality using the AltiSurf 520 profilometer. The results suggest a significant effect of the selected machining parameters and conditions on cutting force reduction and on improved surface quality when cutting forces are low. This research provides a valuable insight into optimizing the machining process for hard steels. Full article
(This article belongs to the Special Issue Emerging Trends and Technologies in Manufacturing Engineering)
12 pages, 842 KB  
Article
Effect of Coffee Grounds as a Bio-Input in Lettuce Cultivation
by Amanda Ayda Garcia Basílio, Mariana Souza Gratão, Geovana Cristina Macedo, Sarah Jamilly Leones Xavier, Maria Eduarda Borges Rodrigues Silva, Luiz Antônio Freitas Soares, Pedro Henrique Lopes Macedo, Talles Eduardo Borges dos Santos and Fábio Santos Matos
Sustainability 2026, 18(2), 649; https://doi.org/10.3390/su18020649 - 8 Jan 2026
Abstract
Coffee grounds can be used in agriculture as a bio-input to enhance soil fertility and biodiversity in the long term. Furthermore, the use of coffee grounds in agriculture is a sustainable practice because it reuses an organic waste product as natural fertilizer and [...] Read more.
Coffee grounds can be used in agriculture as a bio-input to enhance soil fertility and biodiversity in the long term. Furthermore, the use of coffee grounds in agriculture is a sustainable practice because it reuses an organic waste product as natural fertilizer and minimizes the environmental impact resulting from the improper disposal of waste. This study aimed to identify the effects of coffee grounds on the growth and yield of iceberg lettuce plants. The experiment was conducted in a greenhouse using 4 kg of substrate in containers with a 5.356 dm3 capacity, following a completely randomized design in a 2 × 2 factorial arrangement. The primary treatment consisted of plants grown in two types of substrate: soil and sand (01) and soil, sand, and 10% coffee grounds (02). The secondary treatment corresponded to irrigation with water (01) and a 10% coffee ground extract solution (02). Coffee grounds incorporated into the soil increase soil fertility; however, they reduce lettuce growth due to the toxicity of the compounds present and should not be used without prior treatment. Processing coffee grounds into irrigation solutions shows promise due to its high potential for use as an agricultural bio-input in lettuce production. This solution enhances the growth and development of the species, resulting in vigorous plants with market value. Full article
Show Figures

Figure 1

12 pages, 4082 KB  
Article
The Influence of Short-Type Heaters and Their Positions on the Oxygen Concentration in the Growth of 300 mm Single Crystal Silicon by the Czochralski Method
by Yunyun Zhu, Deng Deng, Ruifeng Qin, Zhiyuan Shan, Yang Li and Guohu Zhang
Crystals 2026, 16(1), 45; https://doi.org/10.3390/cryst16010045 - 8 Jan 2026
Abstract
The inevitable introduction of oxygen into Czochralski-method-grown single crystal silicon, facilitated by the use of quartz crucibles, can result in the failure of chips and devices. Both the size and position of the heater exert a significant influence on the oxygen concentration within [...] Read more.
The inevitable introduction of oxygen into Czochralski-method-grown single crystal silicon, facilitated by the use of quartz crucibles, can result in the failure of chips and devices. Both the size and position of the heater exert a significant influence on the oxygen concentration within the Czochralski-method-grown silicon. In this study, a novel short-type heater was designed and evaluated for its effect on melt temperature and oxygen diffusion during crystal growth. The silicon melt temperatures and oxygen diffusion coefficients in an MCZ furnace for several heater settings were simulated, and the results were implemented in experiments. From the examination of the growth process through computation, the heater and its positional adjustments were determined to be effective modulators of oxygen concentration during crystal growth, which was consequently reduced to below 4 ppma (ASTM F121-83). Finally, the simulations were validated experimentally, limitations in production were discussed, and possible improvements were outlined. Full article
(This article belongs to the Section Crystal Engineering)
Show Figures

Figure 1

11 pages, 3460 KB  
Article
Design and Fabrication of a Low-Voltage OPAMP Based on a-IGZO Thin-Film Transistors
by Arturo Torres-Sánchez, Isai S. Hernandez-Luna, Francisco J. Hernández-Cuevas, Cuauhtémoc León-Puertos and Norberto Hernández-Como
Nanomaterials 2026, 16(2), 84; https://doi.org/10.3390/nano16020084 - 8 Jan 2026
Abstract
In the last few years, Thin Film Transistors (TFTs) based on materials such as amorphous Indium–Gallium–Zinc Oxide (a-IGZO) have gained interest in large-area and low-cost electronics due to their high carrier mobility, high on/off current ratio, low off-state current, and steep subthreshold slope. [...] Read more.
In the last few years, Thin Film Transistors (TFTs) based on materials such as amorphous Indium–Gallium–Zinc Oxide (a-IGZO) have gained interest in large-area and low-cost electronics due to their high carrier mobility, high on/off current ratio, low off-state current, and steep subthreshold slope. These characteristics make IGZO TFTs suitable for radio-frequency identification (RFID) tags, analog-to-digital converters (ADCs), logic circuits, sensors, and analog components, including operational amplifiers (OPAMPs). This work presents the implementation and characterization of an OPAMP based on n-type a-IGZO TFTs fabricated on glass substrate. Two previously reported design strategies were integrated: a positive feedback network to increase the output impedance and a topology to enhance the transconductance of the driver transistors, both in the differential input stage. A gain of 26 dB, a bandwidth of 2.4 kHz, a gain–bandwidth product (GBWP) of 48 kHz, and a phase margin of 64° were obtained, which confirms the reliability of the design and the fabrication process. Full article
(This article belongs to the Special Issue Wide Bandgap Semiconductor Material, Device and System Integration)
Show Figures

Figure 1

19 pages, 3055 KB  
Article
Computational Design and Service Learning in Informal Settlement Planning: A Pedagogical Model for Architectural Education
by Fernando Lima, Anna Agnew, Emily Schiedemeyer and Vira Williams
Buildings 2026, 16(2), 265; https://doi.org/10.3390/buildings16020265 - 8 Jan 2026
Abstract
Rapid urbanization in the Global South continues to intensify the growth of informal settlements, challenging architectural education to equip students with methods capable of addressing complex socio-spatial, environmental, and ethical conditions. While computational design and service-learning have each been explored within architectural pedagogy, [...] Read more.
Rapid urbanization in the Global South continues to intensify the growth of informal settlements, challenging architectural education to equip students with methods capable of addressing complex socio-spatial, environmental, and ethical conditions. While computational design and service-learning have each been explored within architectural pedagogy, their systematic integration, particularly in the context of informal settlement planning, remains underdeveloped. This article presents a pedagogical framework that combines pattern language theory, shape grammars, and parametric modeling with service-learning principles within an undergraduate architectural design studio. Implemented in ARC 4025 (Architecture Studio 5) at the O’More College of Architecture and Design, Belmont University, the framework guides students in translating empirical observations of an informal settlement in Ahmedabad, India, into rule-based and generative design systems. The methodology emphasizes process–product reciprocity, enabling students to encode settlement patterns as transformation rules that generate, test, and evaluate coherent urban forms across multiple scales. A detailed case study, Community at Scale, is presented as a proof of method, illustrating how analysis is converted into computational logic across dwelling, block, and neighborhood scales. Rather than proposing a finalized solution, the study demonstrates how computational design can operationalize morphogenetic reasoning within architectural education while remaining grounded in social responsibility. The article concludes by discussing pedagogical implications, situating the approach within urban morphology discourse, and outlining limitations and directions for future research. Full article
(This article belongs to the Special Issue Emerging Trends in Architecture, Urbanization, and Design)
Show Figures

Figure 1

19 pages, 976 KB  
Article
Production and Quality of ‘Smooth Cayenne’ Pineapple as Affected by Nitrogen Fertilization and Types of Plantlets in the Northern Region of Rio de Janeiro State, Brazil
by Denilson Coelho De Faria, Rômulo André Beltrame, Jéssica Morais Cunha, Stella Arndt, Simone de Paiva Caetano Bucker Moraes, Paulo Cesar Dos Santos, Marta Simone Mendonça Freitas, Moises Zucoloto, Silvio de Jesus Freitas, Willian Bucker Moraes, Marlene Evangelista Vieira and Almy Junior Cordeiro de Carvalho
Agronomy 2026, 16(2), 153; https://doi.org/10.3390/agronomy16020153 - 7 Jan 2026
Abstract
This study evaluated the effects of nitrogen fertilization and different types of planting material on the yield and fruit quality of pineapple (Ananas comosus var. comosus) cv. Smooth Cayenne under the edaphoclimatic conditions of the Northern region of Rio de Janeiro [...] Read more.
This study evaluated the effects of nitrogen fertilization and different types of planting material on the yield and fruit quality of pineapple (Ananas comosus var. comosus) cv. Smooth Cayenne under the edaphoclimatic conditions of the Northern region of Rio de Janeiro State, Brazil. The experiment was conducted in a randomized block design, arranged in a factorial scheme with four nitrogen rates, six types of planting material, and two harvest seasons (winter and summer). Based on the results, it can be inferred that slips provided higher yields and heavier fruits, whereas plants derived from crowns and suckers showed lower productivity. Increasing nitrogen rates promoted greater fruit mass and length, higher pulp percentage, and increased production of vegetative propagules. Fruits harvested in the summer showed higher soluble solids content (15.5 °Brix), greater pulp and juice percentages, and lower titratable acidity, which are desirable characteristics for fresh consumption. Despite the seasonal differences, fruit mass ranging from 1.5 to 2.0 kg met commercial standards for both processing and domestic markets. The soluble solids/titratable acidity ratio (15.8) was below the ideal range for fresh consumption. The combination of appropriate planting material and nitrogen fertilization contributes to higher production efficiency, cost reduction, and improved fruit quality. Full article
(This article belongs to the Section Horticultural and Floricultural Crops)
Show Figures

Figure 1

27 pages, 4558 KB  
Review
Integrating Additive Manufacturing into Dental Production: Innovations, Applications and Challenges
by Maryna Yeromina, Jan Duplak, Jozef Torok, Darina Duplakova and Monika Torokova
Inventions 2026, 11(1), 7; https://doi.org/10.3390/inventions11010007 - 7 Jan 2026
Abstract
Additive manufacturing (AM) has emerged as a key enabling technology in contemporary dental manufacturing, driven by its capacity for customization, geometric complexity, and seamless integration with digital design workflows. This article presents a technology-oriented narrative review of additive manufacturing in dental implant production, [...] Read more.
Additive manufacturing (AM) has emerged as a key enabling technology in contemporary dental manufacturing, driven by its capacity for customization, geometric complexity, and seamless integration with digital design workflows. This article presents a technology-oriented narrative review of additive manufacturing in dental implant production, focusing on dominant processing routes, material systems, and emerging research trends rather than a systematic or critical appraisal of the literature. An indicative descriptive analysis of publications indexed in the Web of Science and Scopus databases between 2014 and 2024 was used to contextualize the technological development of the field and identify major research directions. Emphasis was placed on metal powder bed fusion technologies, specifically Selective Laser Melting (SLM) and Direct Metal Laser Sintering (DMLS), which enable the fabrication of titanium implants with controlled porosity and enhanced osseointegration. Ceramic AM approaches, including SLA, DLP, and PBF, are discussed in relation to their potential for aesthetic dental restorations and customized prosthetic components. The publication trend overview indicates a growing interest in ceramic AM after 2020, an increasing focus on hybrid and functionally graded materials, and persistent challenges related to standardization and the availability of long-term clinical evidence. Key technological limitations—including manufacturing accuracy, material stability, validated metrology, and process reproducibility—are highlighted alongside emerging directions such as artificial intelligence-assisted workflows, nanostructured surface modifications, and concepts enabling accelerated or immediate clinical use of additively manufactured dental restorations. Full article
(This article belongs to the Section Inventions and Innovation in Advanced Manufacturing)
Show Figures

Figure 1

17 pages, 2618 KB  
Article
Experimental Study on Mechanism of Using Complex Nanofluid Dispersions to Enhance Oil Recovery in Tight Offshore Reservoirs
by Zhisheng Xing, Xingyuan Liang, Guoqing Han, Fujian Zhou, Kai Yang and Shuping Chang
J. Mar. Sci. Eng. 2026, 14(2), 126; https://doi.org/10.3390/jmse14020126 - 7 Jan 2026
Abstract
Horizontal wells combined with multi-stage fracturing are key techniques for extracting tight oil formation. However, due to the ultra-low permeability and porosity of reservoirs, energy depletion occurs rapidly, necessitating external supplements to sustain production. During the hydraulic fracturing process, large volumes of fracturing [...] Read more.
Horizontal wells combined with multi-stage fracturing are key techniques for extracting tight oil formation. However, due to the ultra-low permeability and porosity of reservoirs, energy depletion occurs rapidly, necessitating external supplements to sustain production. During the hydraulic fracturing process, large volumes of fracturing fluid are injected into reservoirs, increasing its pressure to a certain extent. However, due to the oil-wet nature of the formation, the fracturing fluid cannot penetrate the rock, failing to enhance oil recovery during the shut-in period. Surfactant-based nanofluids have been introduced as fracturing fluid additives to reverse rock wettability, thereby boosting imbibition-driven recovery. Although the imbibition has been studied to inspire the tight oil recovery, few studies have demonstrated the imbibition in enhanced fossil hydrogen energy, which further promotes the imbibition recovery. In this paper, complex nanofluid dispersions (CND) have been proved to enhance the tight reservoir pressure. Through contact angle and imbibition experiments, it is shown that CND can transform oil-wet rock to water-wet, reduce the adhesion of oil, and improve the ultimate oil recovery through the imbibition effect. Then, core flow testing experiments were conducted to show CND can decrease the flow resistance and improve the swept area of the injected fluid. In the end, pressure transmission tests were conducted to show CND can enhance the formation energy and production after fracturing. Results demonstrate that CND enables the fracturing fluid to travel further away from the hydraulic fractures, thus decreasing the depletion of tight formation pressure and maintaining a higher oil production rate. Results help optimize the design of the hydraulic fracturing of tight offshore reservoirs. Full article
(This article belongs to the Special Issue Advances in Offshore Oil and Gas Exploration and Development)
Show Figures

Figure 1

25 pages, 8923 KB  
Review
Mechanisms and Protection Strategies for Concrete Degradation Under Magnesium Salt Environment: A Review
by Xiaopeng Shang, Xuetao Yue, Lin Pan and Jingliang Dong
Buildings 2026, 16(2), 264; https://doi.org/10.3390/buildings16020264 - 7 Jan 2026
Abstract
Concrete structures suffering from Mg2+ environments may suffer severe damage, which mainly has something to do with the coupled effect among Cl, SO42−, and Mg2+. Based on a systematic review of Web of Science and [...] Read more.
Concrete structures suffering from Mg2+ environments may suffer severe damage, which mainly has something to do with the coupled effect among Cl, SO42−, and Mg2+. Based on a systematic review of Web of Science and Scopus database (2000–2025), we first summarized the migration behavior, reaction paths, and interaction mechanism of Cl, SO42−, and Mg2+ in cementitious matrices. Secondly, from the perspective of Cl cyclic adsorption–desorption breaking the passivation film of steel bars, SO42− generating expansion products leads to crack expansion, then Mg2+ decalcifies C-S-H and transforms into M-S-H; we analyzed the main damage mechanisms, respectively. In addition, under the coexistence conditions of three kinds of ions, the “fixation–substitution–redissolution” process and “crack–transport” coupling positive feedback mechanism further increase the development rate of damage. Then, some anti-corrosion measures, such as mineral admixtures, functional chemical admixtures, fiber reinforcements, surface coatings, and new binder systems, are summarized, and the pros and cons of different anti-corrosion technologies are compared and evaluated. Lastly, from two aspects of simulation prediction for the coupled corrosion damage mechanism and service life prediction, respectively, we have critically evaluated the advances and problems existing in the current research on the aspects of ion migration-reaction coupled models, multi-physics coupled frameworks, phase-field methods, etc. We found that there is still much work to be conducted in three respects: deepening mechanism understanding, improving prediction precision, and strengthening the connection between laboratory test results and actual projects, so as to provide theoretical basis and technical support for the durability design and anti-corrosion strategies of concrete in complex Mg2+ environments. Full article
Show Figures

Figure 1

24 pages, 12341 KB  
Review
Toolpath-Driven Surface Articulation for Wax Formwork Technology in the Production of Thin-Shell, Robotic, CO2-Reduced Shotcrete Elements
by Sven Jonischkies, Jeldrik Mainka, Harald Kloft, Bhavatarini Kumaravel, Asbjørn Søndergaard, Falk Martin and Norman Hack
Buildings 2026, 16(2), 257; https://doi.org/10.3390/buildings16020257 - 7 Jan 2026
Abstract
This study introduces a digital fabrication process for producing recyclable, closed-loop wax formwork for architectural concrete applications with visually rich surface articulation while drastically reducing formwork milling time. As such, this paper presents (a) a circular large-scale production method for wax blocks via [...] Read more.
This study introduces a digital fabrication process for producing recyclable, closed-loop wax formwork for architectural concrete applications with visually rich surface articulation while drastically reducing formwork milling time. As such, this paper presents (a) a circular large-scale production method for wax blocks via a single casting process; (b) four machine-time-optimized surface articulation strategies through CNC toolpath-driven design; (c) the investigation of different coating systems to improve architectural concrete surface quality and to ease demolding; and (d) the integration of robotic concrete shotcreting using a low-CO2 fine-grain concrete. For the first time, wax formwork technology, characterized by its waste-free approach, has been combined with robotic shotcreting in a digital and automated workflow to fabricate fiber-reinforced, geometrically complex thin-shell concrete elements with distinct surface articulations. To evaluate the process, a series of four thin-shell concrete elements was produced, employing four distinct parametric toolpath-driven designs: linear surface articulation, crossed surface articulation, topology-adapted curve flow surface articulation, and robotic drill topology-adapted surface articulation. Results revealed a possible reduction in milling time of between 77% and 94% compared to traditional milling methods. The optimized toolpaths and design-driven milling strategies achieved a high degree of visual richness, showcasing the potential of this integrated approach for the production of high-quality architectural concrete elements. Full article
(This article belongs to the Special Issue Robotics, Automation and Digitization in Construction)
Show Figures

Figure 1

14 pages, 1662 KB  
Article
Approach to Design of Potent RNA Interference-Based Preparations Against Hepatocellular Carcinoma-Related Genes
by Petr V. Chernov, Vladimir N. Ivanov, Nikolai A. Dmitriev, Artem E. Gusev, Valeriia I. Kovchina, Ivan S. Gongadze, Alexander V. Kholstov, Maiia V. Popova, Dmitry A. Kudlay, Daria S. Kryuchko, Ilya A. Kofiadi and Musa R. Khaitov
Int. J. Mol. Sci. 2026, 27(2), 603; https://doi.org/10.3390/ijms27020603 - 7 Jan 2026
Abstract
Every year, the scientific community continues to drive advances in healthcare, opening up new perspectives in the treatment and management of various diseases. Despite vast strides being made in the quality of life and longevity, we still face an equally significant growth in [...] Read more.
Every year, the scientific community continues to drive advances in healthcare, opening up new perspectives in the treatment and management of various diseases. Despite vast strides being made in the quality of life and longevity, we still face an equally significant growth in the burden of oncological pathologies. Although current trends lean towards preventive and personalized medicine, numerous hurdles remain to be cleared to develop robust strategies in the field of oncology. Among all types of tumors, one of the prominent positions is occupied by hepatocellular carcinoma (HCC), which is one of the most widespread primary cancers with a high mortality rate. Conventional approaches to HCC therapy, such as surgery or chemotherapy, rarely provide steady performance due to the highly polymorphous nature of the cancerous process. In this study, we suggest an alternative methodological framework for designing potent siRNAs targeting genes implicated in hepatocellular carcinoma, implementing RNA interference mediated by synthetic small interfering RNAs (siRNAs) against mRNAs of ITGB1 and CD47 genes. Products of these genes are renowned drivers of tumor progression. We have developed a software algorithm for the design of unmodified and modified siRNAs, carried out solid-phase synthesis of the most promising molecules, and proved their capability to perform a more than 50-fold suppression of expression of the target genes in vitro. Full article
Show Figures

Figure 1

18 pages, 2219 KB  
Article
Integrative Transcriptomic and Systems Biology Analyses Identify TCB1 as a Calcium-Responsive Gene in Cryptococcus neoformans
by Andrea Gomes Tavanti, Júlia Catarina Vieira Reuwsaat, Heryk Motta, Eamim Daidrê Squizani, Rodrigo Silva Araujo Streit, Patrícia Aline Gröhs Ferrareze, Matheus da Silva Camargo, Bruno Cesar Feltes, Marilene Henning Vainstein, Charley Christian Staats and Lívia Kmetzsch
Microorganisms 2026, 14(1), 122; https://doi.org/10.3390/microorganisms14010122 - 7 Jan 2026
Abstract
Cryptococcus neoformans is a pathogenic yeast and the leading cause of cryptococcosis in humans. The calcium-calcineurin signaling pathway plays a central role in stress adaptation and virulence. To identify the uncharacterized regulators of fungal adaptation, we utilized an integrative systems biology approach, combining [...] Read more.
Cryptococcus neoformans is a pathogenic yeast and the leading cause of cryptococcosis in humans. The calcium-calcineurin signaling pathway plays a central role in stress adaptation and virulence. To identify the uncharacterized regulators of fungal adaptation, we utilized an integrative systems biology approach, combining differential gene expression and network analysis using transcriptomic data from three key components of the calcium-calcineurin pathway (Cna1, Crz1, and Pmc1). Our workflow identified the CNAG_00522 gene product, which we designated tricalbin 1 (TCB1) due to its conserved calcium and lipid-binding C2 domains. TCB1 expression was found to be regulated by both Cna1 and Pmc1. Network analyses positioned Tcb1 as a bottleneck linking general stress response and cellular processes. Further molecular characterization confirmed that TCB1 expression is temperature and calcium-responsive. Functional studies of the tcb1Δ mutant revealed an enlarged capsule, increased GXM shedding, and enhanced viability under host-mimicking conditions. However, phenotypic screening demonstrated that the tcb1Δ mutant does not display sensitivity to cell wall or osmotic stressors, and TCB1 deletion did not attenuate virulence in the Tenebrio larval model. These findings suggest that TCB1 functions as a specialized regulator of fungal growth at 37 °C, capsule size, and GXM shedding. This study validates our integrative approach for guiding the identification of these complex regulators. Full article
Show Figures

Figure 1

11 pages, 258 KB  
Article
Effects of Supplementation with Rumen-Protected Fats and Thermally Processed Soybean on Intake, Nutrient Digestibility, and Milk Composition of Pantaneiras Ewes
by Renata Alves das Chagas, Tatiane Fernandes, Ariadne Patrícia Leonardo, Agda Costa Valério, Núbia Michelle Vieira da Silva, Cláudia Andrea Lima Cardoso, Rui José Branquinho de Bessa and Fernando Miranda de Vargas Junior
Dairy 2026, 7(1), 7; https://doi.org/10.3390/dairy7010007 - 7 Jan 2026
Abstract
This study aimed to evaluate the effect of the supplementation with rumen-protected fat from soybean or palm and thermally processed soybean on the feed intake, digestibility of nutrients, milk production, and milk content of ewes. Twenty-five Pantaneiras ewes, 3–6 years old, 39.8 ± [...] Read more.
This study aimed to evaluate the effect of the supplementation with rumen-protected fat from soybean or palm and thermally processed soybean on the feed intake, digestibility of nutrients, milk production, and milk content of ewes. Twenty-five Pantaneiras ewes, 3–6 years old, 39.8 ± 3.51 kg body weight, and 65 ± 4 days in milk, were distributed into five treatments (5 ewes in each) in a completely randomized design continuous trial, over 56 days. The treatments consisted of daily supplementation with soybean-based rumen-protected fat (SPF; 30 g/d), palm-based rumen-protected fat (PPF; 30 g/d), a blend of soybean and palm rumen-protected fats (Blend; 30 g/d), thermally processed soybean (TPS; 124 g/d), and a control without supplementation. We performed a daily evaluation of feed intake and milk production, and every 14 days, we evaluated the nutrient digestibility, milk composition, and fatty acid profile. The protein and casein content were lower in the SPF treatment. Supplementation with PPF resulted in a higher saturated fatty acid content, while supplementation with TPS resulted in higher monounsaturated and polyunsaturated fatty acid contents. The supplementation with SPF resulted in higher milk fatty acid functionality. Feeding ewes SPF or TPS enhanced nutrient intake and digestibility, leading to increased milk production and an improved milk fatty acid profile. In contrast, supplementation with PPF resulted in a less favorable fatty acid composition. Full article
(This article belongs to the Special Issue Farm Management Practices to Improve Milk Quality and Yield)
39 pages, 6731 KB  
Article
Implementation Pathways for the Sustainable Development of China’s 3D Printing Industry Under the “Dual Carbon” Goals: Policy Optimization and Technological Innovation
by Liuyu Xuan and Yu Zhao
Sustainability 2026, 18(2), 591; https://doi.org/10.3390/su18020591 - 7 Jan 2026
Abstract
This study systematically examines the policy and technological pathways for the sustainable development of China’s 3D printing industry under the “Dual Carbon” goals. A three-dimensional sustainability framework is developed, integrating resource efficiency, environmental performance, and socio-economic value. Based on this framework, the study [...] Read more.
This study systematically examines the policy and technological pathways for the sustainable development of China’s 3D printing industry under the “Dual Carbon” goals. A three-dimensional sustainability framework is developed, integrating resource efficiency, environmental performance, and socio-economic value. Based on this framework, the study conducts a full-process analysis covering design, material preparation, manufacturing, post-processing, use, and recycling stages. The analysis identifies key carbon-reduction mechanisms of 3D printing, including material savings, reduced energy consumption, lightweight-enabled emission reduction, and distributed manufacturing. A comparative analysis of China, the European Union, and the United States reveals major constraints in China’s 3D printing sector, particularly in top-level policy design, standardization systems, legal frameworks, industrial coordination, and low-carbon core technologies. Based on these findings, the study proposes a dual-driven development pathway integrating policy optimization and technological innovation. From an institutional perspective, this pathway emphasizes green policy incentives, including strategic planning, standard setting, green finance, and collaborative governance. From a technological perspective, it highlights the importance of low-carbon material development, refined energy-efficiency management, life-cycle carbon accounting platforms, and value creation across the product life cycle. Overall, the study demonstrates that effective policy–technology synergy is essential for transforming theoretical carbon-reduction potential into scalable and practical outcomes, providing a systematic analytical framework for academic research and actionable guidance for policymakers and industry stakeholders. Full article
Show Figures

Figure 1

Back to TopTop