Search Results (39,307)

Bee-derived products such as apitoxin, royal jelly, propolis, bee pollen, and honey are increasingly being used as part of cosmetic products because all of them contain a large number of bioactive compounds with antioxidant, anti-inflammatory, antimicrobial, and regenerative properties, which enable them to be used for therapeutic purposes. The aim of this investigation was to assess the performance of an automated growth-based system in order to make a quantitative examination of the total aerobic viable counts in bee-derived personal care products using NF-TVC vials that contained a nutrient-based medium with dextrose as the carbon source. According to USP general chapter <1223>, pivotal validation criteria such as linearity, equivalence of results, operative range, precision, accuracy, ruggedness, limit of quantification, and limit of detection have demonstrated that the automated system can be used for a reliable total aerobic viable count. Moreover, the actual research demonstrated that polysorbates efficiently block the antimicrobiological potential of bioactive compounds, such as phenols, flavonoids, enzymes, peptides, and fatty acids, which naturally occur in apitoxin, royal jelly, propolis, bee pollen, and honey, allowing for efficient microorganism recovery from the bee-made products tested. Therefore, this AGBS could be applied efficiently within the cosmetic industry to assess the total aerobic viable count in bee-derived products such as capillary treatments, toothpaste, and anti-aging cream, affording several benefits associated with faster product release into the market. Full article

The continuous emergence of SARS-CoV-2 variants, especially the Omicron strain with its heightened transmissibility, has posed ongoing challenges to the efficacy of existing vaccine and drug regimens. This situation highlights the pressing demand for antiviral drugs employing novel mechanisms of action. Pentacyclic triterpenoids (PTs), a structurally varied group of compounds derived from plants, exhibit both antiviral and anti-inflammatory activities, making them attractive candidates for further therapeutic development. These natural products, along with their saponin derivatives, show broad-spectrum inhibitory effects against multiple SARS-CoV-2 variants (from Alpha to Omicron) via interactions with multiple targets, such as the spike protein, main protease (Mpro), RNA-dependent RNA polymerase (RdRp), and inflammatory signaling pathways. This review consolidates recent findings on PTs and their saponins, emphasizing their influence on the key structural features required for inhibiting viral attachment, membrane fusion, reverse transcription, and protease function. We systematically summarized the structure–activity relationships and their antiviral results of PTs based on different target proteins in existing studies. Furthermore, this work points toward new strategies for designing multi-target PT-based inhibitors with improved efficacy against Omicron and future variants. Full article

Some Sacoglossa sea slugs are capable of stealing and maintaining functional intracellular chloroplasts—kleptoplasts—from their macroalgal prey for periods of up to several months, a process known as kleptoplasty. Although the cultivation of these marine invertebrates under laboratory conditions is crucial for research in various fields (e.g., endosymbiosis, animal physiology, discovery of new marine natural products), rearing protocols are scarce. This study presents a standardized protocol for the laboratory rearing of large numbers of the sacoglossan tropical sea slug Elysia crispata. The detailed protocol successfully facilitated embryonic development, larval metamorphosis, and juvenile-to-adult transition, allowing the rearing of multiple generations. Two groups, characterized by acquiring different kleptoplasts, were obtained by feeding the sea slugs with two different prey macroalgae: Bryopsis sp. and Acetabularia acetabulum. Usually referred to as lettuce sea slug among marine aquarium hobbyists, E. crispata is a highly valued organism for its striking appearance and ability to control nuisance algal growth in reef aquariums. This protocol allows experimental reproducibility and access to specimens under different development stages, potentially boosting research on kleptoplasty while also contributing to reducing the impact of the marine aquarium trade on natural populations. Full article

Safflower (Carthamus tinctorius L.) is increasingly attracting the attention of Mediterranean farmers due to its broad environmental adaptability and low input requirements. Although still relatively underexplored, this species holds remarkable potential as a source of natural dyes and bioactive phytochemicals with recognized health-promoting and phytotherapeutic properties. In this study, the effects of genotype and sowing time on safflower’s productive and qualitative traits were investigated by testing six genotypes and two sowing times (autumn and spring) in an open-field trial conducted in central Tuscany. The Pieve genotype achieved the highest floret dry yield per head, number of heads per plant, and total floret yield per plant, whereas the Montola 2000 genotype was distinguished by its elevated polyphenol concentration and pronounced antioxidant activity. Autumn sowing resulted in higher yields of bioactive pigments, including carthamin and yellow quinochalcones, alongside greater total phenolic content and antioxidant capacity. Conversely, spring sowing appeared to limit pigment biosynthesis, likely due to environmental stressors such as elevated temperature and excessive light exposure. Overall, these findings highlight the strong influence of genotype and sowing time on the accumulation of health-beneficial compounds in safflower. By optimizing these factors, safflower can be strategically valorized as a multipurpose crop in the Mediterranean region, combining economic and environmental sustainability with the production of natural compounds of high nutraceutical and phytotherapeutic value. Full article

Cnidium monnieri (L.) Cusson is a species of Umbelliferae plants, and it is one of China’s traditional medicinal herbs, widely distributed in China owing to its strong adaptability in fields. In this article, the research progress on the taxonomy, distribution, cultivation techniques, active components, analysis methods, antibacterial and insecticidal properties, and ecological applications of C. monnieri was reviewed. The main active components in C. monnieri are coumarins (mainly osthole) and volatile compounds, exhibiting multiple pharmacological effects, e.g., anti-inflammatory, antibacterial, antioxidant, anti-tumor, and immune-regulating effects. Some modern analytical techniques (e.g., HPLC, GC-MS, and UPLC-QTOF-MS) have enabled more precise detection and quality control of these chemical components in C. monnieri. The specific active constituents in C. monnieri (e.g., coumarins and volatile components) exhibit significant inhibitory effects against various pathogenic fungi and insect pests. Simultaneously, the resources provided during its flowering stage (e.g., pollen and nectar) and the specific volatiles released can repel herbivorous insect pests while attracting natural enemies, such as ladybugs, lacewings, and hoverflies, thereby enhancing ecological control of insect pests in farmland through a “push–pull” strategy. Additionally, C. monnieri has the ability to accumulate heavy metals, e.g., Zn and Cu, indicating its potential value for ecological restoration in agroecosystems. Overall, C. monnieri has medicinal, ecological, and economic value. Future research should focus on regulating active-component synthesis, improving our understanding of ecological mechanisms, and developing standardized cultivation systems to enhance the applications of C. monnieri in modernized traditional Chinese medicine and green agriculture production. Full article

As the largest exporter in the global solid tire market, Sri Lanka’s natural rubber supply chain plays a critical role in global production, yet its social dimension remains largely unaddressed. Our study aims to assess the social performance of a Sri Lankan natural rubber supply chain in solid tire manufacturing using social life cycle assessment (S-LCA) in a cradle-to-gate approach. Study adapts “More Good and Less Bad” method which captures both positive and negative social impacts, addressing traditional S-LCAs’ focus on negative impacts solely. It applies to updated methodological sheets to distinguish “good” and “bad” social conditions across subcategories based on baseline compliance. Social impacts were quantified using a Social Performance Index (SPI), calculated by multiplying social performance levels by working hours at the organizational level, comprising SPI_good for good social impacts and SPI_bad for bad social impacts. Data was collected through stakeholder interviews, with working hours calculated using a “working hour model”. Results showed mixed social performance across 39 subcategories, identifying six social hotspots: promoting social responsibility (27.67% less bad, 72.32% more good), wealth distribution (26.87% less bad, 73.13% more good), commitment to sustainability issues (100% less bad), social benefits (100% less bad), safe and healthy living conditions (100% less bad), and hours of work (88.74% less bad, 11.26% more good). Full article

Alzheimer’s disease (AD) is a prevalent neurodegenerative condition that progressively impairs cognitive processes, particularly learning and memory. A key pathological feature of AD involves senile plaques mainly composed of β-amyloid (Aβ) peptides, generated via the amyloidogenic pathway from amyloid precursor protein (APP) through sequential β-secretase (BACE1) and γ-secretase cleavage, positioning BACE1 inhibition as a prime therapeutic target. In this study, we applied bioassay-guided fractionation of the butanol-soluble fraction from Dryopteris crassirhizoma rhizomes, previously reported to inhibit Aβ production, to isolate and characterize Aβ-lowering constituents. Through successive chromatographic steps, nine compounds were isolated and structurally classified into flavonoids, chromones, and phloroglucinols, including epicatechin (1), β-carboxymethyl-(-)-epicatechin (2), 7-methoxy-isobiflorin (3), biflorin (4), eriodictyol (5), noreugenin (6), phloroglucinols (butyrylphloroglucinol (7), 2-propionyl-4-methylphloroglucinol (8), and 2-butyryl-4-methylphloroglucinol (9) by comprehensive spectroscopic analysis (NMR, MS, UV, IR). These compounds were assessed for effects on sAPPβ and BACE1 (β-secretase) levels by Western blot, with Aβ production quantified via ELISA in a cellular AD model (APP-CHO cells). Compounds 5–9 significantly reduced sAPPβ and BACE1 expression while potently suppressing Aβ generation. These results demonstrate that diverse constituents from D. crassirhizoma rhizomes inhibited Aβ production through BACE1 suppression, highlighting their potential as natural lead compounds for AD prevention or therapy. Full article

Agriculture faces increasing challenges in ensuring food security under a changing climate, where abiotic stresses such as salinity and drought represent major constraints to crop productivity. These stresses induce complex physiological and biochemical alterations in plants, including osmotic imbalance, oxidative damage, and disruption of metabolic pathways, ultimately impairing growth and yield. In this context, the application of biostimulants has emerged as a sustainable strategy to enhance plant resilience. While synthetic products are widely available, growing attention is being directed toward natural bio-based products, particularly those derived from renewable biomasses and organic wastes, in line with circular economy principles. This review critically examines the current literature on bio-based products with biostimulant properties, with particular emphasis on vermicompost-derived extracts, humic-like substances, and macro- and microalgae extracts, focusing on their role in mitigating salt and drought stress in plants. The reviewed studies consistently demonstrate that these bio-products enhance plant tolerance to abiotic stress by modulating key physiological and biochemical processes, including hormonal regulation, activation of antioxidant defence systems, accumulation of osmoprotectants, and regulation of secondary metabolism. Moreover, evidence indicates that these bio-based inputs can improve nutrient use efficiency, photosynthetic performance, and overall plant growth under stress conditions. Overall, this review highlights the potential of non-microbial bio-based biostimulants as effective and sustainable tools for climate-resilient agriculture, while also underlining the need for further research to standardize formulations, clarify mechanisms of action, and validate their performance under field conditions. Full article

Lignans are naturally occurring compounds found in a wide variety of plant species, including flaxseed, soybean, pumpkin seed, broccoli, sesame seed, and some berries. Lignans have been used for centuries in both food and traditional herbal medicine. Recently, numerous new lignans and lignan derivatives with diverse biological properties have been identified. Lignans are considered promising for human health due to their hydrogen-donating antioxidant activity together with their ability to complex divalent transition metal cations. They have demonstrated beneficial effects for cardiovascular disease, as well as in maintaining blood glucose levels, supporting cardiac health, promoting anti-obesity effects, decreasing the risk of renal diseases, enhancing brain function, improving skin and gut health, among others. This review explores the biosynthesis and biological effects of lignans, with a particular focus on their antihypertensive and anti-obesity properties, as well as the molecular mechanisms involved. It also highlights recent advances in sustainable lignan extraction techniques that are suitable for human use. The mechanisms underlying these bioactivities are thought to involve hormonal metabolism and availability, antioxidant action, modulation of angiogenesis, and more. However, further research is needed to fully elucidate the molecular pathways through which lignans exert their therapeutic effects. Overall, lignans from various plant sources hold significant potential for application in functional foods, dietary supplements, and pharmaceutical products aimed at preventing and managing a range of health conditions, including hypertension and obesity. Full article

As the global climate discourse shifts from mitigation to achieving net-negative emissions, there is a critical need for replicable, real-world models of climate-positive development at a regional scale, particularly in the Global South. This study addresses this gap by conducting a detailed greenhouse gas (GHG) inventory of four diverse provinces in Thailand and analyzing the results through the newly proposed Climate-Positive Pathways Framework (CPPF). Our findings reveal that all four provinces function as significant net-negative GHG sinks. They achieve this status through three distinct archetypes: a Conservation-Dependent pathway, an Agricultural Frontier pathway, and a novel Agro-Sink pathway. Most significantly, in the Agro-Sink model, we find that in specific economic contexts, managed agricultural landscapes can surpass natural forests as the primary driver of regional carbon removal. This typology provides a new, landscape-scale paradigm for cleaner production, proposing these three archetypes as transferable, evidence-based models for regional policymakers. This underscores that effective climate action requires context-specific regional planning that strategically leverages both natural and agricultural capital. Full article

The abrupt COVID-19 lockdown in early 2020 offered a unique natural experiment to examine vegetation productivity responses to sudden declines in human activity. Although vegetation often responds to environmental changes with time lags, how such lags operate under short-term, intensive disturbances remains unclear. This study combined multi-source environmental data with an interpretable machine learning framework (XGBoost-SHAP) to analyze spatiotemporal variations in net primary productivity (NPP) across the Beijing-Tianjin-Hebei region during the strict lockdown (March–May) and recovery (June–August) periods, using 2017–2019 as a baseline. Results indicate that: (1) NPP showed a significant increase during lockdown, with 88.4% of pixels showing positive changes, especially in central urban areas. During recovery, vegetation responses weakened (65.31% positive) and became more spatially heterogeneous. (2) Integrating lagged environmental variables improved model performance (R² increased by an average of 0.071). SHAP analysis identified climatic factors (temperature, precipitation, radiation) as dominant drivers of NPP, while aerosol optical depth (AOD) and nighttime light (NTL) had minimal influence and weak lagged effects. Importantly, under lockdown, vegetation exhibited stronger immediate responses to concurrent temperature, precipitation, and radiation (SHAP contribution increased by approximately 7.05% compared to the baseline), whereas lagged effects seen in baseline conditions were substantially reduced. Compared to the lockdown period, anthropogenic disturbances during the recovery phase showed a direct weakening of their impact (decreasing by 6.01%). However, the air quality improvements resulting from the spring lockdown exhibited a significant cross-seasonal lag effect. (3) Spatially, NPP response times showed an “urban-immediate, mountainous-delayed” pattern, reflecting both the ecological memory of mountain systems and the rapid adjustment capacity of urban vegetation. These findings demonstrate that short-term removal of anthropogenic disturbances shifted vegetation responses toward greater immediacy and sensitivity to environmental conditions. This offers new insights into a “green window period” for ecological management and supports evidence-based, adaptive regional climate and ecosystem policies. Full article

Port wine production involves the addition of grape spirit to halt fermentation and retain natural sweetness. This spirit, produced by distilling wine and its by-products, must comply with legal standards, including a mandatory sensory assessment. Because grape spirit influences Port wine’s volatile composition, this study investigated the odour-active compounds present in several grape spirits intended for fortification. Volatile compounds were extracted by liquid–liquid extraction, concentrated, and analysed using gas chromatography–olfactometry (GC-O) and gas chromatography–mass spectrometry (GC-MS). In GC-O, based on frequency detection, a panel of assessors sniffed the extracts to determine the presence of aroma compounds. The results revealed a wide range of odour-active compounds in grape spirits, belonging to several chemical families such as esters, alcohols, terpenic compounds and acids. These compounds exhibited both pleasant aromas, such as fruity, floral and caramel notes as well as undesirable ones like cheese and foot odour. Most of these compounds originate from the fermentation process and are also found in other unaged distilled beverages, including young Cognac, Calvados and fruit spirits. This research highlights the aromatic complexity of grape spirits and, for the first time, determined the aroma thresholds for 25 of 36 the compounds studied at an ethanol content of 20%. Full article

Harnessing nature’s ingenuity with microorganisms for industrial production is an attractive solution to today’s climate concerns. Nature’s innate diversity allows the production of many value-added chemicals and can be expanded on through genetic engineering. Although the use of microbial cell factories (MCFs) has been extremely successful at lab scale, the numbers of successful bioprocesses remain limited. High cell densities and long cultivation times lead to reductions in productivity over the course of the cultivation through the effects of genetic and expression instability of the strain. This instability leads to population diversification. In this review, we explore the roots of genetic instability in microorganisms, focusing on prokaryotic bioprocesses, and how organisms cope with this instability. We spotlight single-cell detection methods capable of monitoring populations within the bioprocess both in- and on-line. We also examine different approaches to minimizing population diversification, both through strain development and bioprocess engineering. With this review, we highlight the fact that population-averaged metrics overlook the single-cell stresses driving genetic and functional instability, leading to an overestimation of microbial bioprocess robustness. High-throughput single-cell monitoring in industry-like conditions remains essential to identify and select truly stable microbial cell factories and bioprocesses. Full article

Plants from the Zingiberaceae family, particularly Zingiber officinale, Curcuma longa, and Alpinia galanga, are rich sources of bioactive compounds with documented antidiabetic and anti-inflammatory properties. This review summarizes current evidence on their phytochemical profiles and pathways relevant to metabolic regulation. Key compounds, including gingerols, shogaols, curcuminoids, and phenylpropanoids, support glucose homeostasis by enhancing insulin sensitivity, promoting Glucose Transporter Type 4 (GLUT4)-mediated glucose uptake, improving β-cell function, and modulating metabolic signaling pathways such as PI3K/Akt, AMPK, PPARγ, and NF-κB. Their potent antioxidant and anti-inflammatory activities further reduce oxidative stress and chronic low-grade inflammation, both central to the progression of type 2 diabetes and its complications. Evidence from selected clinical and experimental studies suggests that dietary supplementation with whole-rhizome preparations or standardized extracts (including formulation-enhanced products) may improve fasting blood glucose (FBG), glycated hemoglobin (HbA1c), lipid metabolism, and oxidative stress markers. Recent advances in delivery systems, including nanoemulsions, liposomes, and curcumin–piperine complexes, substantially enhance the bioavailability of poorly soluble phytochemicals, strengthening their therapeutic potential. Overall, Zingiberaceae plants emerge as promising natural supplements in nutritional and pharmacological strategies targeting diabetes. Further clinical research is required to refine dosage, confirm long-term efficacy, and support their integration into evidence-based metabolic interventions. Full article

This study aimed to evaluate the effects of an ethanolic extract from Punica granatum L. (EE-PG) on bovine ovarian tissue vitrification, focusing on follicular morphology, ultrastructure, stromal cell density, collagen distribution, redox status, and mRNA expression of antioxidant-related genes. Bovine ovarian cortex fragments were divided into a fresh control group for in vivo tissue evaluation or vitrified either with the base vitrification solution (αMEM) alone or supplemented with different concentrations of EE-PG (10, 50, and 100 µg/mL), and subsequently stored in liquid nitrogen for 5 days. After warming, fragments were allocated for morphological and oxidative stress analyses or incubated for 24 h to resumption of cellular metabolism. The concentrations of 10 and 100 µg/mL preserved follicular morphology immediately after warming, and were therefore selected for ultrastructural evaluation. Both concentrations mitigated vitrification-induced damage. Gene expression analysis showed decreased levels of catalase (cat), Glutathione Peroxidase 1 (gpx1), and Nuclear Factor Erythroid 2-Related Factor 2 (nrf2) compared with the fresh control, whereas Superoxide Dismutase (SOD) enzymatic activity increased after incubation with 10 µg/mL EE-PG compared with all experimental groups. Moreover, Malondialdehyde (MDA) levels in tissues treated with 10 or 100 µg/mL were comparable to fresh controls after incubation. Overall, EE-PG at 10 or 100 µg/mL in the vitrification solution supported the maintenance of tissue morphology, redox balance—despite the downregulation of essential antioxidant genes, which may be associated with a reduced demand for enzymatic antioxidant defense—and cellular metabolism, indicating potential for improving bovine ovarian tissue vitrification outcomes. Full article