Search Results (933)

This study investigated the effects of Ethephon (CEPA) and Gibberellic acid (GA₃) treatments on the post-harvest flavor quality of ‘Yunning No. 1’ green lemon. A comprehensive analysis was conducted on the changes in primary metabolites (sugars, organic acids, amino acids, alcohols) in the pulp and peel, as well as those in major volatile compounds in the peel during fruit storage. The results showed that CEPA treatment initially increased volatile compounds like monoterpenes and sesquiterpenes in the fruit peel during early storage, but later decreased these compounds along with total sugar and amino acid content in the pulp. Conversely, GA₃ treatment markedly delayed the decline in sugars and organic acids in the fruit peel, preserved the amino acid content in the pulp and the alcohol content in the peel, and delayed the decrease in volatile compound content in the peel. In conclusion, GA₃ treatment effectively delayed the decline in primary metabolites and volatile compounds to maintain the storage quality of green lemon; therefore, GA₃ represents a suitable strategy for the preservation of green lemons. CEPA temporarily improved aroma but accelerated quality deterioration, making it better suited for short-term degreening. This study offers a theoretical foundation for optimizing post-harvest degreening and preservation techniques of green lemons. Full article

The global production of lemons and limes amounts to approximately 16 million tonnes annually, making these fruits among the most significant contributors to global citrus production. The objective of this study was to investigate the potential conversion of citrus waste derived from lemons into functional porous carbon biochar. The results of thermal analysis of the obtained materials provided valuable insights into the carbonization mechanism of the examined raw materials, which offers a sustainable alternative to conventional carbon sources. The physicochemical and thermal properties of the resulting carbon materials were characterized through analysis of phase transformations of carbonates, structural and elemental composition changes resulting from the preceding treatment process, and electrical conductivity. Research demonstrated that the carbonization of the material must be preceded by an oxidation stage, enabling effective reinforcement of the carbon structure. The oxidation process also directly impacts the reduction of other elemental species. Fourier-transform infrared (FTIR) spectroscopy revealed the presence of functional groups that vary in position and intensity depending on the selected process parameters. It was demonstrated that optimal processing conditions encompass preliminary oxidation of the material at 140 °C, followed by carbonization at 700 °C. Full article

Gastric cancer remains a leading cause of cancer-related mortality worldwide. Citrus fruits are rich in polyphenols, exerting antioxidant and chemo-preventive activities, and lemon peel represents a valuable source of such bioactive compounds. Previous studies showed that Citrus limon peel extracts (LPE) inhibited the activity of some enzymes of the antioxidant system and reduced the interleukin-6-dependent invasiveness of gastric and colon cancer cells. In the present study, we have investigated the effects of LPE on the human gastric adenocarcinoma AGS and MKN-28 cells and on the activity of a crucial redox enzyme, catalase (CAT). Indeed, LPE significantly reduced the cell viability and clonogenic potential of the gastric cancer cells and induced morphological changes indicative of cytotoxicity. Moreover, LPE modulated the intracellular redox homeostasis by decreasing levels of the hydrogen peroxide-related reactive oxygen species (ROS) while increasing those of superoxide anions and decreasing levels of superoxide dismutases (SODs). Western blotting analysis revealed that LPE downregulated CAT, SOD-1, SOD-2, and monoamine oxidase A (MAO-A) protein expression level in both cell lines. Finally, the extract inhibited CAT activity in a dose-dependent manner (IC₅₀ = 0.008 ± 0.003 mg/mL; K_i = 0.012 ± 0.002 mg/mL). These findings indicate that LPE exerts cytotoxic and redox-modulating effects through the inhibition of antioxidant enzymes and the alteration of ROS balance. Therefore, the agro-industrial by-product LPE could be considered as a promising natural source of polyphenolic compounds with potential applications in the prevention and therapy of gastric cancer. Full article

The classification of lemons by size is a crucial industrial process that ensures specific quality standards. Lemon sorting is typically performed by hand or often using expensive, outdated machines. In this article, we develop Machine Learning and Queuing algorithms, program them on low-cost hardware—specifically, a microcontroller and a single-board computer—and integrate them with an existing fruit-sorting machine, which classifies lemons by size. We acquired a dataset of 3127 lemon images in six industry-standardized sizes. We developed algorithms to extract geometric features, including one based on the peduncle location, which is estimated using a pre-trained Faster Objects, More Objects (FOMO) model. We used these features to train and evaluate five machine learning models, with the best-performing model achieving 87.22% accuracy over a set of lemons acquired under controlled conditions. We tested the proposed system in a real industrial environment, proving its feasibility by sorting 1558 lemons and obtaining an accuracy of 78.00%, despite the industrial-standard sizes having considerable overlap. Full article

Objectives: The purpose of this study is to compare the differences between lemon-derived exosome-like nanovesicles (LELNs) stored at −80 °C, −20 °C, and 4 °C for one month and freshly isolated LELNs, in terms of characterization and anti-inflammatory effects on periodontal ligament cells, aiming to identify suitable storage conditions for LELNs. Methods: Nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and micro bicinchoninic acid assay (BCA) were conducted to access the characterization differences. LPS-induced human periodontal ligament cells were used as an in vitro inflammatory model, and the changes in biological functions were examined by qRT-PCR and ELISA. Results: LELNs stored at −80 °C retained the highest particle and protein concentration and showed the least aggregation and heterogeneity in size on TEM images, while the average particle sizes shown by NTA were similar. And LELNs exhibited similar anti-inflammatory effects on periodontal ligament cells after one month of storage at −80 °C, −20 °C, and 4 °C. Conclusions: We found that LELNs can maintain in vitro anti-inflammatory ability when stored at either −80 °C, −20 °C, or 4 °C for one month, while storing at −80 °C maintains the concentration and uniform particle size best. Full article

The Citrus limon (L.) Burm. f. industry suffers significant losses due to fungal diseases. Therefore, this study aimed to evaluate the effectiveness of sodium benzoate (SB) and potassium sorbate (PS) on the incidence of fungal decay and fruit quality when used as preharvest treatments on Fino lemon trees over two consecutive seasons (2021–2023). Lower concentrations of SB and PS (0.1% and 0.5%) applied in one or two treatments successfully controlled fungal decay. On average, SB achieved a greater reduction in decay, ranging from 45% to 60%, compared to PS’s reduction of 25% to 50%. This approach minimised the negative impact on lemon fruit quality, in contrast to the highest doses (more than 1%) and the greatest number of applications (more than three times), which increased lemon susceptibility to decay. Furthermore, lemons treated with 0.5% SB twice enhanced antioxidant systems, showing a 35% increase in total phenolic content in the flavedo at harvest compared to the control. Consequently, the application of 0.5% SB twice at preharvest emerges as a promising and potential alternative to conventional fungicides for effective fungal decay control and maintenance of acceptable lemon quality traits during cold storage. Full article

Mal secco disease (MSD), caused by Plenodomus tracheiphilus, poses a serious threat to Citrus limon production across the Mediterranean Basin. This study investigates the potential of high-resolution WorldView-3 imagery for detecting early-stage MSD symptoms in lemon orchards through the integration of three pansharpening algorithms(Gram–Schmidt, NNDiffuse, and Brovey) with two machine learning classifiers (Random Forest and Support Vector Machine). The Brovey-based fusion combined with Random Forest yielded the best results, achieving 80% overall accuracy, 90% precision, and 84% recall, with high spatial reliability confirmed by 10-fold cross-validation. Spectral analysis revealed that Brovey introduced the largest radiometric deviation, particularly in the NIR band, which nonetheless enhanced class separability between healthy and symptomatic crowns. These findings demonstrate that moderate spectral distortion can be tolerated, or even beneficial, for vegetation disease detection. The proposed workflow—efficient, transferable, and based solely on visible and NIR bands—offers a practical foundation for satellite-driven disease monitoring and precision management in Mediterranean citrus systems. Full article

This study focuses on the optimization of modern extraction techniques for selected by-product materials, including apple, lemon, and tangerine peels, and onion skins, using artificial neural network (ANN) models. The extraction methods included ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) with water as the extractant, as well as maceration (MAC) with natural deep eutectic solvents (NADES). Key parameters, such as total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities, including reducing power (EC₅₀) and free radical scavenging capacity (IC₅₀), were evaluated to compare the efficiency of each method. Among the techniques, UAE outperformed both MAE and MAC in extracting bioactive compounds, especially from onion skins and tangerine peels, as reflected in the highest TPC, TFC, and antioxidant activity. UAE of onion skins showed the best performance, yielding the highest TPC (5.735 ± 0.558 mg CAE/g) and TFC (1.973 ± 0.112 mg RE/g), along with the strongest antioxidant activity (EC₅₀ = 0.549 ± 0.076 mg/mL; IC₅₀ = 0.108 ± 0.049 mg/mL). Tangerine peel extracts obtained by UAE also exhibited high phenolic content (TPC up to 5.399 ± 0.325 mg CAE/g) and strong radical scavenging activity (IC₅₀ 0.118 ± 0.099 mg/mL). ANN models using multilayer perceptron architectures with high coefficients of determination (r² > 0.96) were developed to predict and optimize the extraction results. Sensitivity and error analyses confirmed the robustness of the models and emphasized the influence of the extraction technique and by-product type on the antioxidant parameters. Principal component and cluster analyses showed clear grouping patterns by extraction method, with UAE and MAE showing similar performance profiles. Overall, these results underline the potential of UAE- and ANN-based modeling for the optimal utilization of agricultural by-products. Full article

In the present study, the essential oils (EOs) of peels and leaves from the new limonime lime, ‘Eugene’ hybrid, were analyzed for the first time and compared with those of its parental plants, Citrus latifolia var. latifolia (Persian lime) and Citrus × limon cv. Zambetakis (lemon). This hybrid represents the first successful cross between these two species, exhibiting distinctive features such as aroma and shape. GC-MS analysis identified a total of 30 and 44 metabolites in the hybrid’s peel and leaf EOs, respectively. Limonene was the predominant volatile in both peels and leaves across all genotypes. In the peel EOs, the monoterpenes γ-terpinene, β-pinene, and geranial were among the most abundant compounds. In contrast, the leaf EOs showed differences between genotypes: the hybrid and Persian lime had similar volatile profiles dominated by geranial, neral, and neryl acetate, while β-pinene was only detected in lemon. Additionally, the total phenolic content and DPPH radical scavenging activity of the methanolic extracts of peels and leaves were evaluated, and revealed that lemon extracts were richer in phenolic compounds and with higher antioxidant activity compared to those of hybrid and Persian lime. Overall, the development of improved Greek varieties like the ‘Eugene’ hybrid holds significant potential to enrich the genetic diversity of Greek Citrus germplasm and broaden the commercial portfolio of citrus fruits with unique and desirable traits. Full article

This paper tested the use of the surplus efficient frontier (a minimum tracking error portfolio selection method) to select the optimal hedging portfolio that replicates the best Mexican #4 lemon price in a t + 1 and t + 4 week hedging scenario. Using data on the nine most traded agricultural futures in the US from January 2000 to February 2025, we tested hedging effectiveness across 502 futures portfolios in a weekly backtest. The results suggest that a corn and wheat portfolio increases the hedging effectiveness of the lemon price by 0.7033 or 70.33%. A result that, including the impact of trading fees and taxes, leads to a reduction in income risk to a lemon seller in a t + 1 week hedging horizon. The results suggest that a public or private financial institution could take a short position in such a portfolio to provide a hedge at a price that finances the spot/future price difference at minimum cost to Mexican taxpayers. Full article

Background/Objective: Alzheimer’s disease (AD) is a neurodegenerative condition characterized by oxidative stress, neuroinflammation, amyloidogenesis, and tau-related pathology. This study investigated the macronutrient and phytochemical composition of strawberry (S), lemon (L), and lemon juice-assisted strawberry (S/L) extracts and evaluated their neuroprotective efficacy relative to selenium (Se) in an aluminum chloride (AlCl₃)-induced rat model of AD. Methods: Macronutrients and phenolics were quantified in S, L, and S/L, and the extracts were profiled using high-performance liquid chromatography and electrospray ionization tandem mass-spectrometry. Male Sprague–Dawley rats received AlCl₃ with or without S, L, S/L, or Se, and their cognitive performance was assessed using the Morris water maze, Y-maze, and conditioned avoidance tests. Markers of oxidative status, inflammation, cholinergic function, apoptotic signaling, and Wnt3/β-catenin pathway activity were quantified in the brain tissue, and cortico-hippocampal morphology was examined. Results: The S/L extract showed the highest carbohydrate, protein, and lipid content. The total phenolic content was highest in S/L (60.46 mg gallic acid equivalents/g), followed by L (55.08) and S (44.75), with S/L also being the richest in gallic, ellagic, and chlorogenic acids. S/L attenuated AlCl₃-induced cognitive deficits, restored antioxidant status, suppressed neuroinflammation, improved cholinergic indices, modulated apoptotic signaling, and downregulated amyloidogenic and NLRP3 inflammasome markers, consistent with histological evidence of neuronal preservation. Conclusions: Lemon juice-assisted extraction enhanced the macronutrient and phenolic richness and multitarget neuroprotection of strawberries. S/L co-extracts represent promising functional food–derived adjuvants for AD management and support integrative compositional–mechanistic profiling to optimize natural product–based interventions. Full article

The development of plant-based synbiotic beverages is gaining increasing attention as consumers seek sustainable, functional alternatives to dairy products. This preliminary study investigated the fortification of tibicos (water kefir) with lemon catnip (Nepeta cataria var. citriodora) hydrolate, an aromatic distillation byproduct rich in bioactive terpenoids. After 72 h-fermentation of tibicos, physicochemical, microbiological, health-promoting and sensory parameters were evaluated. Both control and fortified beverages exhibited typical fermentation kinetics, including a decrease in pH, reduction of soluble solids, and accumulation of organic acids. Lactic acid bacteria count remained stable, while yeast proliferation was slightly reduced in the hydrolate-fortified sample, consistent with the known yeast-sensitive nature of certain hydrolate-derived terpenoids. Importantly, hydrolate fortification significantly enhanced antioxidant capacity (DPPH: +34%; ABTS: +39%; RP: +38%). Enzyme-inhibitory activities also increased significantly in the hydrolate-fortified samples (α-Amylase and α-Glucosidase inhibition rates increased by 9% and 11%, respectively). ACE inhibition similarly increased from 32% to 44%, indicating an enhanced antihypertensive potential. HMG-CoA reductase inhibition increased from 31% to 42%, showing improved hypolipidemic activity. Sensory evaluation indicated improved sensory acceptability, imparting citrus–floral notes that balanced the acidic profile of tibicos. These findings highlight the potential of valorizing lemon catnip hydrolate as a functional fortifier in non-dairy synbiotic beverages. Full article

Background/Objectives: Lemon oil has demonstrated its therapeutic effects in various dermatological diseases, and different dosage forms have been investigated to incorporate lemon oil. Using the existing ointment bases to prepare drug-loaded formulations is a common practice in formulation development. Eucerin and Aquaphor are commonly used ointment bases in pharmacy for compounding preparations to meet patient needs. The maximum drug-loading capacity is an important consideration in order not to compromise desired properties of the ointment bases. The objective of this research was to determine the maximum lemon oil loading capacities by understanding the effects of loading lemon oil into Eucerin and Aquaphor on the rheological properties of the lemon oil-loaded formulations. Methods: Lemon oil was incorporated into Eucerin and Aquaphor at different concentrations. The rheological properties of the formulations were determined in flow and oscillation modes. Creep and recover tests were performed to determine the viscoelastic properties of the formulations. Results: The results showed that addition of lemon oil impacted rheological properties, including viscosity, elastic modulus, viscous modulus, compliance, and strain%, but the overall rheological behaviors remained consistent with the control for both bases. Conclusions: The maximum loading capacity of lemon oil could be determined from rheological testing. The rheological studies provided an important tool to determine the desired formulations for topical applications, without affecting the performance characteristics of the commercial products. Full article

Citrus fruit cultivation and processing are constantly rising due to the increasing market demand and diverse utilization potentials. This generates large quantities of residues, predominantly composed of citrus peels. This study aimed to evaluate six different citrus peels using rapid and/or nondestructive instrumental analytical techniques such as ATR-FTIR spectroscopy, spectrophotometric assays, image textural analysis and physicochemical parameter determination. Image textural features managed to discriminate citrus peels based on their structure uniformity, which was found increased in lemon (C. limon) and yellow grapefruit (C. paradisi), whereas clementine (C. clementina) and red grapefruit (C. paradisi) images exhibited an increased non-uniformity of the structure. Physicochemical parameters provided insights into the quality characteristics of citrus peels, while their high ascorbic acid content seems to enhance their antioxidant activity. The obtained results from phenolic and flavonoid content determination indicated a high concentration of polyphenols in the peels, which is aligned with the ATR-FTIR spectra absorption bands. Furthermore, the spectrophotometric assays’ strong correlation suggests that the antioxidant activity of citrus peels is mainly attributed to polyphenols. Ultimately, a chemometric model was employed to provide a comprehensive understanding of the analytical methods’ interactions. Hence, citrus peels’ significant biochemical and, consequently, economic value can be highlighted, underscoring the importance of further research. Full article