Search Results (80)

In this paper, we present a system built on Hyperledger Fabric (HLF) that leverages Confidential Computing (CC) technologies to strengthen data privacy guarantees beyond those achievable through application-level mechanisms alone. While HLF natively supports data confidentiality through Private Collections (PCs), which restrict data visibility to a subset of authorized network participants, these mechanisms do not protect data at the hardware level: a privileged or compromised hosting platform can access plaintext data in memory and on the filesystem irrespective of HLF access control policies. To address this limitation, we integrate CC into HLF by adopting Intel Software Guard Extensions (SGX) in conjunction with the Gramine framework. This integration enables the execution of HLF components—peer nodes, orderers, Chaincodes and client applications—within Trusted Execution Environments (TEEs). Furthermore, to securely grant access to selected data to a trusted third-party software (TPS) external to the blockchain network, we leverage the Remote Attestation (RA) feature provided by CC, as streamlined by Gramine and enforced on a per-request basis, ensuring that only verified enclaves (or “SGX enclaves”) with expected measurements may access private data. In addition, the Sealing mechanism is employed to persistently store cryptographic material required by HLF components on the filesystem while preserving both confidentiality and integrity. Together, PCs, RA, Sealing, and enclave-based execution establish a layered privacy guarantee: PCs enforce application-level data segregation among channel participants; RA provides measurement-based access control for an external TPS; Sealing ensures that cryptographic material and blockchain state remain encrypted on the filesystem; and enclave-based execution protects data in use through hardware-level memory encryption. The proposed system has been applied and experimentally validated in a logistics use case in the Port of Genoa: benchmarks against an experimental HLF deployment demonstrate an average 95th-percentile (p95) performance overhead of approximately 1.3× attributable to SGX memory encryption and Gramine-based enclave execution, whereas an elevated memory usage footprint (33–35 GB per organization) has been measured, mainly due to the Gramine environment: this remains an open direction for future work. Full article

Peptide deformylase (PDF) belongs to a conserved enzyme family critical for N-terminal methionine excision (NME), an essential protein maturation process in prokaryotes and eukaryotic organelles (chloroplasts, mitochondria). To explore the potential functions of OsPDFs in Oryza sativa, this study employed bioinformatics approaches and experimental validation to systematically identify and analyze the OsPDF gene family. Three OsPDF genes (OsPDF1A, OsPDF1B, OsPDF1B2) were identified in rice. These genes are exclusively distributed on chromosome 1. The biophysical properties of these proteins showed that OsPDF1A and OsPDF1B are alkaline proteins, while OsPDF1B2 is acidic, and all are hydrophilic with moderate thermostability potential. Synteny analysis revealed closer evolutionary relationships between Oryza sativa and the monocot Triticum aestivum than with dicots, reflecting conserved PDF function in gramineous plants. Analysis of cis-acting elements in the 2000 bp upstream region of OsPDF gene promoters revealed numerous elements associated with abiotic stress response and hormone regulation. Furthermore, quantitative real-time PCR (qRT-PCR) data supported these findings, indicating that OsPDF1A and OsPDF1B were upregulated under low-temperature stress, and all three OsPDF genes were transcriptionally activated by heat, salt and UV-B stresses, indicating their active involvement in rice growth, development, and abiotic stress tolerance. In summary, OsPDFs exhibit significant functions in rice’s stress adaptation, growth, and development. This study not only enhances our understanding of the OsPDF gene family’s genomic, evolutionary, and functional characteristics, but also provides new perspectives and foundational data for further exploring their regulatory mechanisms in protein maturation and abiotic stress responses, as well as their potential applications in rice stress tolerance breeding. Full article

Different crops have varying effects on soil factors, and their associated microbial community compositions also differ. Currently, there is limited comparative research on crops with distant phylogenetic relationships, such as those between gramineous and leguminous species. In this study, a pot experiment combined with high-throughput sequencing was conducted to enable a detailed comparison of microbial communities and soil factors across four crops: wheat, soybean, and two maize varieties. Compared to leguminous crops, differences between gramineous crops may be relatively smaller. The results showed that among the gramineous and leguminous crops, soybean had the lowest effect on soil electrical conductivity (EC) and available phosphorus (AP) (121.68 ± 2.70, 34.74 ± 1.02). The dominant fungi and bacteria phyla were Ascomycota and Proteobacteria; both were most abundant in the ZD958 variety, at 75.12% and 30.47%, respectively. The fungal diversity of ZD958 was most similar to that of W998, whereas the bacterial diversity of XY335 more closely resembled that of SB13. EC and AP were the key factors influencing fungal community composition, while alkali-hydrolyzable nitrogen (AN) was the key factor affecting bacterial community composition. These findings provide a basis for further in-depth research. Full article

Cervical cancer (CC) remains a common malignant tumor that seriously threatens women’s health globally. Gramine (GR), a natural alkaloid derived from plants such as Arundo donax L., exhibits anti-tumor activities, yet its mechanistic actions in CC are still unclear. Here, we integrated cell-based assays, network pharmacology, and multi-omics analysis to systematically investigate the molecular mechanisms underlying GR’s anti-CC effects. In vitro experiments showed that GR significantly inhibited proliferation and migration, induced apoptosis, and triggered G₀/G₁ phase cell cycle arrest in HeLa cells. Integrated multi-omics analysis identified CDK2 as a critical target of GR, with both mRNA and protein levels markedly reduced following treatment. Mechanistically, GR likely suppresses CC progression by modulating the “CYP4A22-AS1/LINC00958–hsa-miR-133b–CDK2” competitive endogenous RNA (ceRNA) axis. Immune analysis indicated positive correlations of CDK2, CYP4A22-AS1, and LINC00958 with the immune checkpoint molecule CD47. Collectively, our findings demonstrate that GR inhibits CC through a ncRNA-mediated suppression of CDK2, leading to reduced HeLa cell proliferation and migration and enhanced apoptosis. These results provide a mechanistic rationale for developing GR as a candidate agent for targeted therapy and immuno-combination strategies in CC. Full article

Uniform spraying by conventional plant protection drones often results in low herbicide utilization efficiency and environmental contamination, both of which are critical issues in agricultural production. To address these challenges, this study proposed a precision weed management system for maize fields that combines an improved YOLOv11n-OSAW detection model with DJI drones for variable-rate herbicide application. The YOLOv11n-OSAW model was enhanced with Omni-dimensional Dynamic Convolution (OD-Conv), the SEAM attention mechanism, a lightweight ADown module, and the Wise-IoU (WIoU) loss function, aiming to improve the detection accuracy of small and occluded weeds in maize fields. When the model was deployed on an uncrewed aerial vehicle (UAV) operating at 5 m altitude, it achieved mean Average Precision mAP@0.5 values of 97.8% and 97.0% for gramineous and broad-leaved weeds, respectively—representing increases of 2.9 and 1.6 percentage points over the baseline YOLOv11n model. Weed distribution maps generated from the detection results were used to develop site-specific herbicide prescription maps, guiding the drone to implement targeted spraying. Water-sensitive paper analysis verified that the system ensured effective droplet deposition and uniform coverage across different application rate areas. This integrated workflow, covering UAV image acquisition, weed detection, variable-rate application, and effect assessment, reduced herbicide consumption by 20.25% compared with conventional uniform spraying (450 L/ha) while maintaining excellent weed control efficiency and reducing environmental risks. The findings demonstrate that the proposed system provides a practical and sustainable solution for weed management in maize fields. Full article

Herbaceous plant species form the plant synusia of desert ecosystems and play crucial roles in wind-breaking, sand-fixing, and the maintenance of oasis ecosystem stability. This study focused on the leguminous species Medicago sativa and Astragalus laxmannii and the gramineous species Elymus dahuricus, Agropyron desertorum, Agropyron mongolicum and Agropyron cristatum. These are the plants commonly used for ecological restoration in the desertification regions of northwestern China. We conducted a pot experiment with six soil moisture gradients (15% [ck], 12%, 10%, 8%, 6%, and 4%) to simulate drought conditions. We studied how varying degrees of drought stress influenced growth, functional traits and drought resistance, aiming to elucidate the mechanisms by which plants respond to drought. The study indicated that: (1) Drought stress directly and indirectly reduced both the seedling emergence rate and plant height. As soil water content decreased, the seedling emergence rate and plant height declined across all species. (2) The leaves and roots of the plants adopted different strategies to cope with drought stress. As soil water content decreased, leaf water content, leaf succulence, and leaf tissue density increased, while specific leaf area and leaf biomass decreased. Additionally, the root-shoot ratio increased, whereas root biomass, total root length, root surface area, and root volume all decreased. (3) Root system adaptation is the key factor influencing the drought resistance of plants. Drought resistance varied among the six species. Medicago sativa, Elymus dahuricus, and Agropyron cristatum exhibited stronger water retention and more stable growth under drought stress, making them better suited for ecological restoration in arid regions. Our study elucidates differentiation strategies for coping with drought stress and offers essential parameters and theoretical support for species selection and rational assemblage in the ecological restoration of arid regions. Full article

Cyhalofop-butyl is a gramineous herbicide with good control effect, but it causes some damage when used in foxtail millet fields. Brassinolide (BR) is a type of plant growth hormone that can enhance the stress resistance of crops and plays a crucial role in eliminating and alleviating herbicide damage. To investigate the alleviating effect of BR on cyhalofop-butyl damage in foxtail millet, a study was conducted using Jingu 21 as the test material, combining pot experiments and field experiments. All test treatments were sprayed with cyhalofop-butyl at a concentration of 67.5 g a.i./ha. Three BR spraying times were set: the same day as cyhalofop-butyl spraying (D1), one day later (D2), and three days later (D3). Four BR concentrations were set—0 mg/L (C0), 0.05 mg/L (C1), 0.1 mg/L (C2), and 0.2 mg/L (C3)—resulting in a total of 12 treatments. The results showed that after BR spraying, all agronomic trait indicators of Jingu 21 in both pot and field experiments were alleviated. Compared with the control treatment, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) increased to varying degrees, the malondialdehyde (MDA) content decreased, and the drug damage level was alleviated to different extents. In addition, spraying BR can increase the yield of Jingu 21 under cyhalofop-butyl herbicide damage. The results of all indicators indicated that spraying BR one day after cyhalofop-butyl spraying had the best effect. Therefore, spraying BR at a concentration of 0.1 mg/L can effectively alleviate the damage of Jingu 21 plants. It is recommended that when using BR to alleviate damage in foxtail millet, the application should be spaced one day apart from the herbicide spraying. Full article

Orchard grass intercropping offers a promising strategy to mitigate forage scarcity and boost fruit yield. However, its applicability in high-altitude regions such as the Xizang Plateau remains poorly understood. During the four-year experiment, the ecosystem service values (ESV) of an apple orchard intercropped with different grass species in Linzhi, southeast Xizang, were investigated in order to assess the applicability of orchard grass planting at high altitudes and identify optimal grass species combinations. Seven treatments were established, including six artificial grass systems (annual legume, annual gramineous, annual legume + gramineous, perennial legume, perennial gramineous, perennial legume + gramineous) and a natural grass control group. Results showed that artificial grass systems, particularly perennial ones, yielded higher total ESV than the natural grass control. Perennial grasses outperformed annual grasses and natural grass in provisioning services (with higher fruit and forage yields) and regulating services (with enhanced carbon sequestration and oxygen production). However, perennial grasses also led to reduced supporting services, primarily due to decreased soil nutrient availability (especially available phosphorus and potassium) and lower plant diversity. The optimal treatments were Dactylis glomerata monoculture and Medicago sativa + D. glomerata mixed culture, which achieved the highest total ESV. Notably, the nutrient depletion observed in perennial grass treatments highlighted the need for supplemental fertilization to ensure long-term sustainability of the system. In conclusion, artificial orchard grass systems significantly enhanced total net ESV in high-altitude regions, whereas individual ecosystem services demonstrated divergent responses to different grass species. Specifically, D. glomerata monoculture and M. sativa + D. glomerata intercropping emerged as the optimal orchard grass patterns in Linzhi. To maintain long-term orchard productivity, adaptive fertilizer management strategies are recommended to counteract potential soil nutrient depletion associated with these grass cultivation systems. Full article

In recent years, we have observed a growing interest in molecular hybridization, which involves combining chemically and pharmacologically diverse fragments into a single molecule. In this study, we designed and synthesized a series of indole–pyrazole hybrids, variously substituted at the pyrazole ring. The compounds were characterized by spectroscopic methods, and the structures of most of them were confirmed by X-ray analysis. Reactions of 3-(dimethylaminomethyl)indole with bromo-methyl-pyrazole derivatives proceeded in a tautomer-selective mode: the 4-bromo-3(5)-methyl-((1H-pyrazol-1-yl)methyl)-1H-indole tautomers, obtained from the 4-bromo-3-methyl-1H-pyrazole, could be isolated by column chromatography. In contrast, the 3-bromo-5-methyl-1H-pyrazole yielded the ((5-bromo-3-methyl-1H-pyrazol-1-yl)methyl)-1H-indole as the dominant reaction product. The 3-bromo-5-methyl tautomer could not be isolated nor could its presence be identified in solution. However, traces of it were recognized in the crystal of 5-bromo-3-methyl tautomer as a binary solid solution. In silico studies provided the physicochemical parameters of all compounds, enabling the estimation of some derivatives affinity to certain enzymes. In vitro evaluation of the hemolytic and cytoprotective properties of all derivatives showed that most of the compounds exhibited no hemolytic activity, while all demonstrated significant cytoprotective effects on human erythrocytes under oxidative stress. Full article

Given the pressing global food security crisis and climate change-induced constraints on agricultural productivity, crop rotation proves critical for boosting yield and grain quality of winter wheat (Triticum aestivum) alongside ameliorating soil quality. However, the legacy effect of different preceding crops on synergistic increments of wheat productivity and soil fertility remains to be fully clarified. Five different preceding crop–winter wheat rotations were conducted in a field experiment established in Huanghua, China. Maize (Zea mays), sorghum (Sorghum bicolor), and millet (Setaria italica) were designated as preceding gramineous crops, and soybean (Glycine max) and mung bean (Vigna radiata) were assigned as preceding legume crops. Grain yield, protein fraction, and soil nutrients were measured to elucidate the legacy effect of the preceding crops on the subsequent winter wheat. Leguminous predecessors significantly evaluated the grain yield of winter wheat compared to gramineous predecessors, particularly that the mung–winter wheat rotation (Mun-W) was 11.56% higher than that of the maize–winter wheat rotation (Mai-W). This rising yield was attributed to the increase of 4.05% in spike number per hectare and 14.31% in kernel number per spike. The Mun-W facilitated the highest gluten protein content (8.22%) in winter wheat among five treatments, which was 6.06% higher than that in the sorghum–winter wheat system. Soil organic matter (SOM) showed an advantage in legume–winter wheat rotations (Leg-Ws) compared to gramineous crop–winter wheat systems (Gra-Ws). Notably among these, the Mun-W significantly enhanced SOM content by 0.99% relative to the Mai-W. The soybean–winter wheat system decreased soil pH by 0.36 compared to the Mai-W system. Coupling coordination degree (CCD) and co-benefit index (CBI) in the Leg-Ws exhibited significant superiority of 62.41% and 42.22% over the Gra-Ws, respectively, and the Mun-W attained maximum CCD by 0.84 and CBI by 0.77. From a multi-objective assessment perspective of the legacy effect of the preceding crops, legume-based rotations facilitate synergistic improvements of yield, protein quality, and soil nutrients in winter wheat. Full article

This study aimed to evaluate the impact of intercropping Brassica oleracea. with three perennial grasses (Poa pratensis L., Lolium perenne L., and Festuca rubra L.) under varying levels of iron (Fe) availability (Fe0, Fe1 and Fe5) in nutrient solutions. The research focused on biomass accumulation, photosynthetic efficiency, root development, nutrient uptake, and oxidative stress response. In the absence of Fe, Brassica sp. exhibited chlorosis, reduced biomass, and increased ferric chelate reductase (FCR) enzyme activity as an adaptive response. Brassica plants intercropped with Poa sp. maintained higher chlorophyll (Chl) levels and photosystem II efficiency (F_v/F_m values), mitigating Fe deficiency effects. Catalase activity and polyphenol production varied with intercropping species, indicating differential stress response mechanisms. Intercropping improved Zn, Mn, and P accumulation, with Poa sp. facilitating greater Zn and Mn uptake. Intercropping Brassica sp. with specific grass species offers potential agronomic benefits by improving Fe use efficiency, mitigating stress, and enhancing nutrient uptake. Future research should focus on optimizing intercropping combinations for sustainable agricultural practices. Full article

Arundo donax L. has been introduced in markets worldwide due to its economic value. However, it is listed in the world’s 100 worst alien invasive species because it easily escapes from cultivation, and forms dense monospecific stands in riparian areas, agricultural areas, and grassland areas along roadsides, including in protected areas. This species grows rapidly and produces large amounts of biomass due to its high photosynthetic ability. It spreads asexually through ramets, in addition to stem and rhizome fragments. Wildfires, flooding, and human activity promote its distribution and domination. It can adapt to various habitats and tolerate various adverse environmental conditions, such as cold temperatures, drought, flooding, and high salinity. A. donax exhibits defense mechanisms against biotic stressors, including herbivores and pathogens. It produces indole alkaloids, such as bufotenidine and gramine, as well as other alkaloids that are toxic to herbivorous mammals, insects, parasitic nematodes, and pathogenic fungi and oomycetes. A. donax accumulates high concentrations of phytoliths, which also protect against pathogen infection and herbivory. Only a few herbivores and pathogens have been reported to significantly damage A. donax growth and populations. Additionally, A. donax exhibits allelopathic activity against competing plant species, though the allelochemicals involved have yet to be identified. These characteristics may contribute to its infestation, survival, and population expansion in new habitats as an invasive plant species. Dense monospecific stands of A. donax alter ecosystem structures and functions. These stands impact abiotic processes in ecosystems by reducing water availability, and increasing the risk of erosion, flooding, and intense fires. The stands also negatively affect biotic processes by reducing plant diversity and richness, as well as the fitness of habitats for invertebrates and vertebrates. Eradicating A. donax from a habitat requires an ongoing, long-term integrated management approach based on an understanding of its invasive mechanisms. Human activity has also contributed to the spread of A. donax populations. There is an urgent need to address its invasive traits. This is the first review focusing on the invasive mechanisms of this plant in terms of adaptation to abiotic and biotic stressors, particularly physiological adaptation. Full article

This study investigates the potential role of Acrocalymma dark septate endophytic (DSE) fungi in promoting the growth of Solanum lycopersicum (tomato). Recognized as important symbionts that enhance plant growth and resilience under stress, particularly Acrocalymma species, DSE fungi were the focus of this investigation. Specifically, four stains isolated from gramineous plant roots (Acrocalymma sp. E00677, Acrocalymma vagum E00690, Acrocalymma chuxiongense E01299A, and Acrocalymma chuxiongense E01299B) were examined. Morphological characteristics were observed using three different media, confirming typical DSE traits such as dark pigmentation and septate hyphae. Phylogenetic analysis using six genetic markers (ITS, LSU, SSU, tef1, rpb2, and tub2) placed the strains within the Acrocalymma genus. Co-culture test and physiological index measurements showed that all strains significantly enhanced root development, as evidenced by an increased root-to-shoot ratio and a higher number of lateral roots. Additionally, the Acrocalymma DSE strains elevated chlorophyll a, chlorophyll b, and total chlorophyll content, suggesting improved photosynthetic efficiency. Anthocyanin levels were also increased in the tomato leaves, indicating enhanced antioxidative defense mechanisms. Among these strains, Acrocalymma vagum E00690 exhibited the most substantial effect on root activity. The widespread presence of 325 Acrocalymma isolates from 25 countries underscores its broad ecological adaptability. These findings suggest that Acrocalymma DSE fungi positively influence tomato growth, with potential implications for improving plant resilience under environmental stress. This study highlights the importance of further exploring DSEs, particularly Acrocalymma fungi, to better understand their ecological roles in agricultural practices, particularly in tomato cultivation. Full article

A series of novel hybrid uracil derivatives incorporating the natural alkaloids caffeine or gramine, linked via 1,2,3-triazole ring, were synthetized using click chemistry. The structures of the obtained compounds were confirmed by spectroscopic methods, including ¹H NMR, ¹³C NMR, FT-IR, and mass spectrometry. The biological activity of hybrids was evaluated in vitro, including assessments of hemolytic activity, antioxidant potential, antifungal efficacy, and antibacterial activity. Additionally, molecular docking studies were conducted in silico for the most active antioxidant candidate. The results revealed that the hemocompatibility of the derivatives was structure-dependent. While caffeine-containing hybrids exhibited moderate-to-low cytoprotective activity under oxidative stress conditions, those incorporating gramine showed significantly higher potency. A plausible molecular mechanism underlying their cytoprotective activity is proposed. Several compounds also inhibited the growth of the plant pathogens Fusarium culmorum and Botrytis cinerea. The promising antioxidant and antifungal properties of selected uracil–alkaloid hybrids highlight their potential as multifunctional bioactive compounds for managing oxidative stress and controlling plant pathogens. Furthermore, the finding demonstrates the effectiveness of click chemistry as a versatile tool for the synthesis of bioactive heterocyclic compounds. Full article

Wheat dwarf virus (WDV) poses significant threats to gramineous crops, making it crucial to explore its codon usage patterns and evolutionary dynamics for effective disease control. This study analyzed ten WDV isolates, including two from triticale (WDVT_117 and WDVT_118), using metrics such as relative synonymous codon usage (RSCU), effective number of codons (ENC), codon adaptation index (CAI), and codon bias index (CBI). Neutrality plots, ENC-plots, and PR2-plots were employed to assess the role of mutation and selection. Results revealed weak codon preference in triticale-derived strains (CAI: 0.145–0.269; CBI: −0.042–0.111; ENC > 40), with hierarchical GC content. Neutrality analysis and ENC-plot distributions indicated natural selection as the dominant force, supported by T/C bias at the third codon position (PR2-plot). Shared optimal codons UUC and UAC in highly expressed genes may imply a potential significant role in virus adaptation. RSCU-based clustering and MP phylogenetic analysis revealed that WDVT strains form a distinct cluster with elevated genetic diversity, potentially driven by genomic recombination in the synthetic host. These findings demonstrate that WDVT balances mutational constraints and host adaptation through selective codon optimization. This study provides a foundation for codon-based antiviral research and the development of agricultural strategies to combat WDV infections. Full article