Search Results (1,090)

Soursop fruit (Annona muricata L.) exhibits a rapid loss of firmness during postharvest ripening, mainly attributed to pectin depolymerization and cell wall restructuring. Among the enzymes involved, rhamnogalacturonan lyase (RGL), belonging to the PF06045 protein family, contributes to the degradation of rhamnogalacturonan I (RG-I), a key structural component of pectin. However, the regulatory mechanisms and transcriptional dynamics of RGL genes in tropical fruits remain poorly characterized. This study aimed to evaluate RGL in three soursop varieties (GUANAY-1, GUANAY-2, and GUANAY-3) during postharvest ripening through integrative bioinformatic, transcriptional, and enzymatic analyses. Bioinformatic analysis identified five soursop genes containing the PF06045 domain, designated RGL1–RGL5, which were grouped into three phylogenetic clusters. Differential expression analysis revealed that RGL1, RGL2, and RGL3 were differentially expressed, while functional enrichment analysis indicated that these genes are mainly associated with lyase activity and cell wall polysaccharide disassembly. Quantitative polymerase chain reaction (qPCR) revealed variety-dependent transcriptional patterns. RGL2 showed expression peaks on day 5 in GUANAY-1 and GUANAY-3 and on day 7 in GUANAY-2, while RGL3 reached its maximum expression on day 5 in all varieties. Enzymatic activity also varied among varieties, showing concordance with RGL2 and RGL3 expression in GUANAY-1, a delayed maximum in GUANAY-2, and a progressive decline in GUANAY-3. Principal component analysis explained 87.2% of the total variation, with enzymatic activity contributing mainly to PC1 and RGL2 and RGL3 expression to PC2. Overall, these results demonstrate differential regulation of RGL among soursop varieties and confirm its central role in RG-I degradation during postharvest fruit softening. Full article

Strawberry is a non-climacteric fruit with a short postharvest shelf life. Recently, edible coatings have attracted the attention of the food industry. Cellulose is the most abundant carbohydrate polymer on Earth, and is also a renewable natural material, biocompatible with food. This work aimed to evaluate the postharvest quality of strawberries coated with edible coatings based on hydroxypropylmethylcellulose (HPMC) and bacterial nanocellulose (BNC) and its functionalization, using vegetal extracts with reported antifungal activity. Five treatments were applied on postharvest strawberries: C (control, with no coating); Cel (HPMC:BNC in a 95:5 ratio); EPAC (cellulose + Persicaria acuminata extract); EO (cellulose + Pelargonium graveolens essential oil) and CBZ (cellulose + carbendazim). Weight, firmness, total soluble solids, titratable acidity, ripe index, respiration rate, ethylene production rate, and natural fungal incidence were measured. Furthermore, the C and Cel fruit surface was observed by SEM. Cel and EPAC treatments proved to be beneficial in maintaining the quality of the treated fruit during storage. Both coatings contributed to a lower weight loss and firmness. They also decreased the respiratory rate and the natural fungal incidence, delaying the senescence of the treated strawberries. These treatments can be alternatives to extend strawberry life postharvest. Full article

This study evaluated the fruit quality of 12 apple cultivars grafted onto the cold-resistant dwarfing interstock SH40 in the arid region of Lingwu, Ningxia, to identify well-adapted varieties for local production. A total of 21 indicators were measured, encompassing three major aspects: external quality (e.g., fruit size, shape index, peel color), internal flavor (e.g., soluble solids, soluble sugars, titratable acids, vitamin C content), and textural attributes (e.g., hardness, crispness, chewiness), and data were analyzed using principal component analysis and membership function methodology. The cultivars exhibited distinct quality profiles under identical management: ‘Red General’ performed well in fruit size, weight, and sugar–acid balance; ‘Yanfu 6’ showed the highest firmness and crispness; ‘Shengli’ had the greatest soluble solids content; and ‘Granny Smith’ was richest in vitamin C. Four principal components were extracted, explaining 80.06% of the total variance and simplifying the quality evaluation system. Based on the comprehensive membership function scores, ‘Red General’, ‘White Winter Pearmain’, and ‘Huashuo’ ranked highest in overall fruit quality. In conclusion, these three cultivars perform excellently on SH40 and are recommended for promotion, whereas ‘Red Delicious’ is not recommended due to poor performance. These findings offer a practical reference for selecting apple cultivars paired with SH40 in similar arid regions. Full article

Honeyberry (Lonicera caerulea L.) is a recently domesticated fruit crop, and there has been rather limited research on its phenological characteristics. In this study, we comprehensively evaluated the phenological and fruit quality traits of a honeyberry germplasm collection comprising 45 accessions. The annual growth period of the 45 accessions ranged from 153 (part of Russian accessions) to 173 days (part of Japanese accessions) in Harbin, China. The accessions of Japanese origin (‘Ri–5’, ‘Ri–68’, ‘RiM–3’, ‘Riwan’, ‘Riwandianlan’, and ‘Riwan–13’) consistently exhibited delayed flowering and ripening, as well as higher single fruit weights and fruit firmness. In contrast, the accessions of Russian and Chinese origins displayed relatively early to mid-season phenology, along with higher levels of vitamin C, anthocyanins, and soluble solids. Furthermore, there were great coefficients of variation (CVs) in total anthocyanins (CV = 35%), flavonoids (CV = 30%), phenolics (CV = 21%), and titratable acidity (CV = 19%) among the 45 accessions. Principal component analysis and hierarchical clustering revealed distinct clustering patterns among Japanese accessions. Among these accessions, ‘RiM–3’ exhibited both a relatively large fruit size and high firmness, implying its potential to overcome the inevitable trade-off between fruit size and firmness typically observed in Chinese and Russian honeyberry accessions. Our investigation and findings provide valuable information for honeyberry breeding aimed at optimizing the maturity period and enhancing fruit quality, as well as a reference for the current cultivation and management methods. Full article

Melatonin (MEL), also known as N-acetyl-5-methoxytryptamine, has been reported in plants as a secondary messenger involved in regulating abiotic stress responses. MEL treatment, either preharvest or postharvest, regulates several physiological and biochemical processes during fruit growth and ripening in horticultural products. These include reproductive development, tissue and quality maintenance, delayed senescence, and responses to abiotic stress. Due to its natural origin, low toxicity, and multifunctional regulatory capacity, MEL has recently attracted attention as a promising ‘green preservative’ for sustainable postharvest management. Additionally, MEL coordinates through cross-talk with other plant hormones, such as abscisic acid, ethylene, polyamines, jasmonic acid, γ-aminobutyric acid, salicylic acid, and nitric oxide, to regulate postharvest ripening and senescence. Furthermore, MEL enhances antioxidant systems and improves membrane integrity, thereby alleviating chilling injury and enhancing fruit firmness and colour. Notably, recent evidence highlights the innovative regulatory mechanisms of MEL involving redox homeostasis, hormone signalling reprogramming, and transcriptional modulation of stress-responsive pathways. MEL could therefore be considered an emerging, eco-friendly tool for prolonging the shelf-life of fruit and vegetables and maintaining their quality. This review summarises the mechanisms by which MEL contributes to plant stress resistance by regulating the biosynthesis and metabolism of stress tolerance and improving fruit quality. Full article

The use of unconventional water sources, such as those from marine desalination plants, is challenging for agriculture due to boron concentrations exceeding 0.5 mg L⁻¹, which can impact crop yield and quality. To ensure sustainability, it is crucial to understand crop responses to high boron levels and to develop strategies to mitigate its toxic effects. This study evaluated the impact of irrigation with a nutrient solution containing 15 mg L⁻¹ of boron on tomato plants (Solanum lycopersicum L.). To modulate the physiological effects of boron toxicity, two biostimulant products based on an extract from the brown alga Laminaria digitata and other active ingredients were applied foliarly. Agronomic, nutritional, and metabolic parameters were analyzed, including total yield, number of fruits per plant, and fruit quality. Additionally, mineral analysis and metabolomic profiling of leaves and fruits were performed, focusing on amino acids, organic acids, sugars, and other metabolites. A control treatment was irrigated with a nutrient solution containing 0.25 mg L⁻¹ of boron. The results showed that a boron concentration of 15 mg L⁻¹ significantly reduced total yield by 45% and significantly decreased fruit size and firmness. Mineral and metabolomic analyses showed significant reductions in Mg and Ca concentrations, significant increases in P and Zn levels, excessive boron accumulation in leaves and fruits, and significant changes in metabolites associated with nitrogen metabolism and the Krebs cycle. Biostimulant application did not significantly improve agronomic performance, likely due to high boron accumulation in the leaves, although significant changes were detected in leaf nutritional status and metabolic profiles. Full article

Apple breeding programs focus on enhancing yield, quality, and disease resistance, with a strong emphasis on evaluating phenological traits like flowering time and pomological traits such as fruit size and flavour, which are crucial for commercial success and consumer preference. Twenty-four families were obtained by crossing six apple varieties selected as pollen receptors and four apple genotypes resistant to scab selected as pollen donors. Data related to bud burst date, flowering date, harvest date, lengths of the periods between bud burst and flowering and from flowering to harvest (developmental period), fruit equatorial and polar diameter, fruit polar/diameter ratio, soluble solid content (SSC) and flesh firmness were analysed as a genetic partial diallel design. The study’s ANOVA on 24 fruit families across two years revealed significant genotype–environment interactions affecting flowering date, harvest date, and developmental periods, with some variables like fruit weight and soluble solids showing consistent variation. During each year, temperature influenced phenological phases, with earlier budbreak and flowering in warmer, less variable conditions in 2019. Analysis of genetic effects indicated high heritability for phenological traits and moderate heritability for fruit morphology and quality, with parental genetic contributions varying over years. Principal component and Procrustes analyses identified key variable groupings and parent profiles, highlighting genotypes such as ‘Granny Smith’, ‘McIntosh’, and ‘HM100’ with consistent additive effects, and certain families with notable heterotic performance. Overall, genetic and environmental interactions significantly shape phenological and fruit quality traits, guiding breeding strategies. Full article

This study evaluates the feasibility of establishing a new strawberry cultivation zone in the Region of Florina, Northern Greece, as a strategy to support rural revitalization and agricultural diversification. Day-neutral strawberry genotypes were cultivated under net-house conditions at the University of Western Macedonia and assessed for physiological traits (SPAD index, chlorophyll fluorescence) and fruit quality (weight, color, firmness, °Brix, titratable acidity); while postharvest behavior was evaluated after seven days of cold storage. Statistical analysis identified genotypes with superior physiological performance and storability. Preliminary economic analysis suggests that their adoption could increase growers’ income by 20–30% compared to conventional varieties. The findings support the development of a strawberry production zone in Florina, with broader implications for sustainable agricultural intensification and rural development in underutilized European regions. Full article

As cold-sensitive fruits, loquats easily develop chilling injury (CI) during cold storage, which leads to quality deterioration and economic losses. Our prior research indicated that exogenous melatonin (MT) treatment can mitigate CI in postharvest loquats by regulating reactive oxygen species (ROS) metabolism, but the underlying molecular mechanism remains unclear. The primary objective of this study is to decipher the molecular regulatory pathway by which MT alleviates CI in postharvest loquats, focusing on the role of MYB transcription factors (TFs) in modulating antioxidant enzyme genes. Here, MT treatment remarkably reduced CI severity in loquat fruits, as reflected by lower CI index, reduced cell membrane permeability, decreased firmness, lower a* and b* values, and higher L* value, compared with the control group. Moreover, a cold-induced MYB TF, designated EjMYB15, was identified. Compared to non-treated fruits, the expression level of EjMYB15 was maintained at higher levels in MT-treated loquats. Subcellular localization and transactivation assays demonstrated that EjMYB15 is a nuclear-localized transcriptional activator. Electrophoretic mobility shift assay (EMSA) and dual-luciferase reporter (DLR) assays showed that EjMYB15 binds the MYB-binding sites (MBS) in the promoters of four antioxidant enzyme genes (EjCAT1, EjCAT2, EjGST1, and EjGST2), thereby activating their transcription. Taken together, these findings indicate that EjMYB15 positively regulates cold tolerance of loquat fruits by improving ROS scavenging capacity. These results elucidate the regulatory pathway by which MYB TFs mitigate CI and provide new theoretical support for the application of MT in alleviating CI in postharvest fruits. Full article

At present, conventional cold storage facilities in China are poorly suited to on-farm storage demands for agricultural produce, mainly due to their large spatial requirements, complex and labor-intensive installation procedures, limited portability, and insufficient coverage in rural areas. These limitations significantly contribute to post-harvest losses of perishable crops such as cherry tomatoes. To address this challenge, the present study proposes a compact and temporary cold storage system—gas-inflated membrane cold storage (GIMCS)—which exploits the inherent safety, cost-effectiveness, ease of deployment, and adaptability of inflatable membrane structures. A series of mechanical performance tests, including tensile strength, pressure resistance, and burst tests, were conducted on PA/PE (Polyamide/Polyethylene) composite membranes. The optimal configuration was identified as a membrane thickness of 70 μm, a gas column width of 2 cm, and a PA/PE composition ratio of 35%/65%. Thermal performance evaluations further revealed that filling the inflatable structure with 100% CO₂ yielded the most effective insulation. Through structural optimization, a cotton-filled gas-inflated membrane cold storage system (CF-GIMCS) incorporating a dual insulation strategy—combining intra-membrane and extra-membrane insulation—was developed. This multilayer configuration significantly reduced conductive and convective heat transfer, resulting in enhanced thermal performance. A comparative evaluation between GIMCS and a conventional cold storage system of equivalent capacity was conducted over a 15-day storage period, considering construction cost, temperature uniformity, and fruit preservation quality. The results showed that the construction cost of GIMCS was only 38% of that of conventional cold storage. The internal temperature distribution of GIMCS was highly uniform, with a maximum horizontal temperature difference of 1.4 °C, demonstrating thermal stability comparable to conventional systems. No statistically significant differences were observed between the two systems in key post-harvest quality indicators, including weight loss and respiration rate. Notably, GIMCS exhibited superior performance in maintaining fruit firmness, with a hardness of 1.30 kg·cm⁻² compared to 1.26 kg·cm⁻² in conventional storage, indicating a potential advantage in shelf-life extension. Overall, these findings demonstrate that GIMCS represents an affordable, technically robust, and portable cold storage solution capable of delivering preservation performance comparable to—or exceeding—that of conventional cold storage. Its modularity, mobility, and ease of relocation make it particularly well suited to the operational and economic constraints of smallholder farming systems, offering a practical and scalable pathway for improving on-farm cold chain infrastructure. Full article

Highbush blueberry (Vaccinium corymbosum L.) is a major berry crop valued for its nutritional and bioactive properties. This study evaluated the influence of cultivation systems and genotypes on fruit quality and antioxidant potential in a two-factorial field experiment (four cultivars × four systems). ‘Sunrise’, ‘Draper’, ‘Ozark Blue’, and ‘Aurora’ were assessed for physicochemical traits, total polyphenols (TPC), vitamin C, nitrates, and antioxidant capacity (2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation decolourisation (ABTS•⁺), 2,2-diphenyl-1-picrylhydrazyl (DPPH•), and ferric-reducing antioxidant power (FRAP))). The maximum fruit weight was recorded in cv. Aurora grew under the raised-bed with agrotextile system (353 g per 100 berries), while Draper produced the smallest fruits (227 g). Soluble solids ranged from 12.2 to 16.9 °Brix, acidity from 0.53 to 0.97 g/100 g FW, and TPC from 318 to 544 mg/100 g FW. Agrotextile treatments stabilised microclimate and reduced stress, resulting in lower ABTS (17.9 vs. 24.0), DPPH (19.8 vs. 22.3), and FRAP (11.6 vs. 13.9 mmol TE/100 g FW) values, indicating stronger radical scavenging activity. Ozark showed the highest TPC, vitamin C (123 mg/1000 g FW), and firmness (420 g/mm), whereas Aurora and Sunrise had brighter fruits (L = 37.6–36.1). Nitrate concentrations remained low (42–68 mg/1000 g FW). Genotype × system interactions significantly influenced secondary metabolite synthesis and stress adaptation. Raised beds with agrotextile improved fresh-market quality, while traditional systems favoured storage stability, providing practical, sustainable cultivation guidelines. Full article

Mechanical damage and microbial contamination are major challenges in the postharvest logistics of perishable fruit. In this study, two types of functionally modified chitosan-based aerogel pads were developed to enhance cushioning and preservation of wax apples. A chitosan/polyvinyl alcohol (CP) aerogel was first optimized by adjusting solid content, CS:PVA ratio, and crosslinker concentration. The optimal formulation (2% solids, 1:1 CS: PVA, 3% glutaraldehyde) exhibited a uniform porous structure and improved compressive strength. A chitosan/montmorillonite (CM) aerogel with 5% montmorillonite (MMT) showed high porosity, low density, and excellent cyclic stability. Incorporating 10% copper nanoparticle-loaded antibacterial fibers (CuNPs-TNF) into CM aerogels yielded CM-Cu aerogels with enhanced cushioning and antimicrobial properties. Under simulated transport and cold storage conditions, all aerogel-packaged groups reduced mechanical damage and decay of wax apples. Compared to the control, the CM-Cu group showed 66% lower decay, 5% less weight loss, 6 N greater firmness, 7% less juice yield, and a 13% reduction in relative electrical conductivity. Additionally, it better preserved fruit color and total soluble solids, extending shelf life by 4 d at 20 °C. These results demonstrate the potential of chitosan-based aerogels as multifunctional packaging materials that combine mechanical protection with antimicrobial activity for perishable fruit preservation. Full article

Packaging is essential for protecting, distributing, and trading fresh fruit. Antimicrobial packaging, which incorporates natural or synthetic bioactive compounds, can inhibit microbial growth, extend shelf life, and reduce reliance on synthetic fungicides. This study aimed to evaluate the effect of allyl isothiocyanate (AITC), released from black mustard seeds, on the quality and fungal development of ‘Burlat’ sweet cherries during postharvest storage under modified atmosphere. The in vitro and in vivo antimicrobial activity of AITC, released from different amounts of mustard seeds in an ‘Inbox’ system, was compared with fludioxonil, a synthetic fungicide authorised for postharvest use on stone fruits in the European Union. The impact of these treatments on weight loss, headspace gas composition, fruit decay, physicochemical and microbiological quality was also analysed. Results showed that AITC inhibited the in vitro growth of Cladosporium cladosporioides, Monilinia laxa and Penicilium expansum, and significantly reduced Alternaria alternata, Botrytis cinerea, and Geotrichum candidum after 96 h at 25 °C and 99% RH. Treatment with 100 mg of mustard seeds achieved rot control comparable to fludioxonil, while maintaining higher firmness and delaying skin darkening after 28 days. Overall, natural AITC from mustard seeds appears to be a promising alternative for preserving sweet cherry quality. Full article

Chilling injury (CI) is a major constraint to the postharvest shelf-life of tomato fruit (Solanum lycopersicum L.), leading to severe quality deterioration, which is closely linked to cell membrane damage. While individual postharvest treatments with the elicitors, such as methyl salicylate (MeSA), methyl jasmonate (MeJA), and the nitric oxide donor sodium nitroprusside (SNP), alleviate cold stress, their synergistic potential in combination has not been systematically explored. The present study evaluated the postharvest efficacy of 1 mM MeSA, MeJA, SNP, and their combinations (MeSA + MeJA, MeSA + SNP, MeJA + SNP) on ‘Vivalto RZ’ tomato fruits stored for 20 days at 2 °C, followed by a 2-day shelf-life period at 20 °C. All treatments effectively mitigated quality loss and CI incidence compared to the control. Notably, the MeSA + SNP and MeJA + SNP combinations demonstrated the highest efficacy, resulting in the lowest CI indices (1.2–1.4 vs. 3.4 in control) and the best preservation of firmness and titratable acidity. This enhanced tolerance was correlated with improved membrane integrity (lower malondialdehyde content and ion leakage) and the strongest upregulation of the antioxidant system, specifically achieving the highest ascorbate peroxidase (APX) activity. The combined application of MeSA or MeJA with SNP exerts a synergistic effect that provides superior chilling tolerance, representing a highly effective and commercially viable strategy to extend the postharvest shelf-life of tomato fruit. Full article

While previous research suggests that innovation capability can enhance sustainable operational performance in sustainable supply chain management practices, empirical insights into the mechanisms underlying this relationship remain limited. Drawing on dynamic capability theory, this study investigates how innovation capability influences sustainable operational performance within the context of Alternative Food Networks (AFNs). Utilizing matched survey data and objective performance metrics from 276 fruit and vegetable preserving and specialty food manufacturing firms in China, the study employed Partial Least Squares Structural Equation Modeling (PLS-SEM) with bootstrapping techniques to test the mediating roles of business platforms and community building. The findings reveal that novelty-centered innovation capability has a significant positive effect on sustainable operational performance, with business platforms serving as a partial mediator in this relationship. In contrast, value-centered community building neither directly influences sustainable operational performance nor mediates the effect of innovation capability. Furthermore, the mediating effect of business platforms is found to be stronger than that of community building. This research presents a novel empirical framework that distinguishes the operational effectiveness of digital platforms in social community building within AFNs, providing managers with a strategic roadmap for prioritizing innovation investments to achieve sustainability. Full article