Search Results (971)

Botrytis cinerea is a highly versatile pathogenic fungus, causing significant damage across a wide range of plant species. A central focus of this review is the recent advances made through proteomics, an advanced molecular tool, in understanding the mechanisms of B. cinerea infection. Recent advances in mass spectrometry-based proteomics—including LC-MS/MS, iTRAQ, MALDI-TOF, and surface shaving—have enabled the in-depth characterization of B. cinerea subproteomes such as the secretome, surfactome, phosphoproteome, and extracellular vesicles, revealing condition-specific pathogenic mechanisms. Notably, in under a decade, the proportion of predicted proteins experimentally identified has increased from 10% to 52%, reflecting the rapid progress in proteomic capabilities. We explore how proteomic studies have significantly enhanced our knowledge of the fungus secretome and the role of extracellular vesicles (EVs), which play key roles in pathogenesis, by identifying secreted proteins—such as pH-responsive elements—that may serve as biomarkers and therapeutic targets. These technologies have also uncovered fine regulatory mechanisms across multiple levels of the fungal proteome, including post-translational modifications (PTMs), the phosphomembranome, and the surfactome, providing a more integrated view of its infection strategy. Moreover, proteomic approaches have contributed to a better understanding of host–pathogen interactions, including aspects of the plant’s defensive responses. Furthermore, this review discusses how proteomic data have helped to identify metabolic pathways affected by novel, more environmentally friendly antifungal compounds. A further update on the advances achieved in the field of proteomics discovery for the organism under consideration is provided in this paper, along with a perspective on emerging tools and future developments expected to accelerate research and improve targeted intervention strategies. Full article

Candidiasis arises from the proliferation of Candida species in the human body, especially in individuals with compromised immune systems. Efficient therapeutic management of candidiasis is often hampered by the limited availability of potent antifungal drugs and the emergence of drug-resistant strains. We have previously identified the N-[(4-sulfamoylphenyl)methyl][1,1′-biphenyl]-4-carboxamide to have fungistatic and fungicidal properties, likely due to the hydrophobic biphenyl–chemical features affecting the structural organization of Candida spp. cell membrane. Here, we designed and synthesized a novel series of twelve 5-arylfuran-2-carboxamide derivatives bearing a new hydrophobic tail as bioisosteric replacement of the diphenyl fragment. Its antifungal effectiveness against C. albicans, C. glabrata, and C. parapsilosis, including ATCC and clinically isolated strains, was assessed for all compounds. The most active compound was N-benzyl-5-(3,4-dichlorophenyl)furan-2-carboxamide (6), with fungistatic and fungicidal effects against C. glabrata and C. parapsilosis strains (MIC = 0.062–0.125 and 0.125–0.250 mg/mL, respectively). No synergistic effects were observed when combined with fluconazole. Interestingly, fluorescent microscopy analysis after staining with SYTO 9 and propidium iodide revealed that compound 6 affected the cell membrane integrity in C. albicans strain 16. Finally, carboxamide 6 exhibited a dose-dependent cytotoxicity on erythrocytes, based on assessing the LDH release. Full article

The increasing use of pharmaceutically active compounds (PhACs), endocrine-disrupting compounds (EDCs), and personal care products (PCPs) has led to the widespread presence of organic micropollutants (OMPs) in aquatic environments, posing a significant global challenge for environmental conservation. In recent years, advanced materials-based nanofiltration (NF) technologies have emerged as a promising solution for water and wastewater treatment. This review begins by examining the sources of OMPs, as well as the risk of OMPs. Subsequently, the key criteria of NF membranes for OMPs are discussed, with a focus on the roles of pore size, charge property, molecular interaction, and hydrophilicity in the separation performance. Against that background, this review summarizes and analyzes recent advancements in materials such as metal organic frameworks (MOFs), covalent organic frameworks (COFs), graphene oxide (GO), MXenes, hybrid materials, and environmentally friendly materials. It highlights the porous nature and structural diversity of organic framework materials, the advantage of inorganic layered materials in forming controllable nanochannels through stacking, the synergistic effects of hybrid materials, and the importance of green materials. Finally, the challenges related to the performance optimization, scalable fabrication, environmental sustainability, and complex separation of advanced materials-based membranes for OMP removal are discussed, along with future research directions and potential breakthroughs. Full article

The formation and evolution of haze layers in planetary atmospheres play a critical role in shaping their chemical composition, radiative balance, and optical properties. In the outer solar system, the atmospheres of Titan and the giant planets exhibit a wide range of compositional and seasonal variability, creating environments favorable for the production of complex organic molecules under low-temperature conditions. Among them, Uranus—the smallest of the ice giants—has, since Voyager 2, emerged as a compelling target for future exploration due to unanswered questions regarding the composition and structure of its atmosphere, as well as its ring system and diverse icy moon population (which includes four possible ocean worlds). Titan, as the only moon to harbor a dense atmosphere, presents some of the most complex and unique organics found in the solar system. Central to the production of these organics are chemical processes driven by low-energy photons and electrons (<50 eV), which initiate reaction pathways leading to the formation of organic species and gas phase precursors to high-molecular-weight compounds, including aerosols. These aerosols, in turn, remain susceptible to further processing by low-energy UV radiation as they are transported from the upper atmosphere to the lower stratosphere and troposphere where condensation occurs. In this review, I aim to summarize the current understanding of low-energy (<50 eV) photon- and electron-induced chemistry, drawing on decades of insights from studies of Titan, with the objective of evaluating the relevance and extent of these processes on Uranus in anticipation of future observational and in situ exploration. Full article

Concern over the presence of pharmaceutical waste in the environment has prompted research into the management of emerging organic micropollutants (EOMs). In response, sustainable technologies have been applied as alternatives to reduce the effects of these contaminants. This study investigated the capacity of filamentous fungi isolated from iron mine soil in the Amazon region to biodegrade the drug chloroquine diphosphate. An initial screening assessed the growth of four fungal strains on solid media containing chloroquine diphosphate: Trichoderma pseudoasperelloides CBMAI 2752, Penicillium rolfsii CBMAI 2753, Talaromyces verruculosus CBMAI 2754, and Penicillium sp. cf. guaibinense CBMAI 2758. Among them, Penicillium sp. cf. guaibinense CBMAI 2758 was selected for further testing in liquid media. A Box–Behnken factorial design was applied with three variables, pH (5, 7, and 9), incubation time (5, 10, and 15 days), and chloroquine diphosphate concentration (50, 75, and 100 mg·L⁻¹), totaling 15 experiments. The samples were analyzed by gas chromatography–mass spectrometry (GC-MS). The most effective conditions for chloroquine biodegradation were pH 7, 100 mg·L⁻¹ concentration, and 10 days of incubation. Four metabolites were identified: one resulting from N-deethylation M1 (N4-(7-chloroquinolin-4-yl)-N1-ethylpentane-1,4-diamine), two from carbon–carbon bond cleavage M2 (7-chloro-N-ethylquinolin-4-amine) and M3 (N1,N1-diethylpentane-1,4-diamine), and one from aromatic deamination M4 (N1-ethylbutane-1,4-diamine) by enzymatic reactions. The toxicity analysis showed that the products obtained from the biodegradation of chloroquine were less toxic than the commercial formulation of this compound. These findings highlight the biotechnological potential of Amazonian fungi for drug biodegradation and decontamination. Full article

Macrocyclic organic nanostructures have emerged as crucial components of functional supramolecular materials owing to their unique structural and chemical features, such as their distinctive “infinite” cyclic topology and tunable topology-dependent properties, attracting significant recent attention. However, the controlled synthesis of macrocyclic compounds with well-defined compositions and geometries remains a formidable challenge. On-surface synthesis, capable of constructing nanostructures with atomic precision on various substrates, has become a frontier technique for exploring novel macrocyclic architectures. This review summarizes the recent advances in the on-surface synthesis of macrocycles. It focuses on analyzing the synthetic mechanisms and conformational characterization of macrocycles formed through diverse bonding interactions, including both covalent and non-covalent linkages. This review elucidates the intricate interplay between the thermodynamic and kinetic factors governing macrocyclic structure formation across these bonding types and clarifies the critical influence of the reaction temperature and external conditions on the cyclization efficiency. Ultimately, this study offers design strategies for the precise on-surface synthesis of larger and more flexible macrocyclic compounds. Full article

In recent decades, the rapid expansion of the food processing industry has led to significant losses and waste, with the fruit and vegetable sector among the most affected. According to the Food and Agriculture Organization of the United Nations (FAO), losses in this category can reach up to 60%. Vegetable waste includes edible parts discarded during processing, packaging, distribution, and consumption, often comprising by-products rich in bioactive compounds such as polyphenols, carotenoids, dietary fibers, vitamins, and enzymes. The underutilization of these resources constitutes both an economic drawback and an environmental and ethical concern. Current recovery practices, including their use in animal feed or bioenergy production, contribute to a circular economy but are often limited by high operational costs. In this context, fermentation has emerged as a promising, sustainable approach for converting vegetable by-products into value-added food ingredients. This process improves digestibility, reduces undesirable compounds, and introduces probiotics beneficial to human health. The present review examines how fermentation can improve the nutritional, sensory, and functional properties of plant-based foods. By presenting several case studies, it illustrates how fermentation can effectively valorize vegetable processing by-products, supporting the development of novel, health-promoting food products with improved technological qualities. Full article

Plant by-products have gained significant attention due to their rich content in bioactive compounds, which exhibit promising antioxidant, antimicrobial, and antitumor properties. In European countries, vegetable waste generation ranged from 35 to 78 kg per capita in 2022, highlighting both the scale of the challenge and the potential for valorization. This review provides an overview of key studies investigating the potential of plant residues in biomedicine, highlighting their possible contents of antioxidant compounds, their antimicrobial and antitumor properties, as well as their applications in dermocosmetics and nutraceuticals. However, despite their potential, several challenges must be addressed, such as the standardization of extraction protocols, as bioactive compound profiles can vary with plant source, processing conditions, and storage methods. Effective segregation and storage protocols for household organic waste also require optimization to ensure the quality and usability of plant by-products in biomedicine. Emerging 4.0 technologies could help to identify suitable plant by-products for biomedicine, streamlining their selection process for high-value applications. Additionally, the transition from in vitro studies to clinical trials is hindered by gaps in the understanding of Absorption, Distribution, Metabolism, and Excretion (ADME) properties, as well as interaction and toxicity profiles. Nonetheless, environmental education and societal participation are crucial to enabling circular bioeconomy strategies and sustainable biomedical innovation. Full article

Understanding the differential impacts of emission sources of volatile organic compounds (VOCs) on formaldehyde (HCHO) levels is pivotal to effectively mitigating key photochemical radical precursors, thereby enhancing the regulation of atmospheric oxidation capacity (AOC) and ozone formation. This investigation systematically selected and analyzed year-long VOC measurements across three urban zones in Shenzhen, China. Photochemical age correction methods were implemented to develop the initial concentrations of VOCs before source apportionment; then Positive Matrix Factorization (PMF) modeling resolved six primary sources: solvent usage (28.6–47.9%), vehicle exhaust (24.2–31.2%), biogenic emission (13.8–18.1%), natural gas (8.5–16.3%), gasoline evaporation (3.2–8.9%), and biomass burning (0.3–2.4%). A machine learning (ML) framework incorporating Shapley Additive Explanations (SHAP) was subsequently applied to evaluate the influence of six emission sources on HCHO concentrations while accounting for reaction time adjustments. This machine learning-driven nonlinear analysis demonstrated that vehicle exhaust nearly always emerged as the primary anthropogenic contributor in diverse functional zones and different seasons, with gasoline evaporation as another key contributor, while the traditional reactivity metric method, ozone formation potential (OFP), tended to underestimate the role of the two sources. This study highlights the primacy of strengthening emission reduction of transportation sectors to mitigate HCHO pollution in megacities. Full article

Bisphenols (BPs), particularly bisphenol A (BPA) and its structural analogues, are synthetic compounds widely used in plastics and industrial materials. These substances are also recognised as endocrine-disrupting chemicals (EDCs) due to their ability to interfere with hormonal systems, which has significant implications for aquatic organisms. This review summarises the occurrence, environmental distribution, and toxicity of BPs in fish, with a focus on estrogenic, androgenic, thyroid, and glucocorticoid disruptions. Studies consistently show that exposure to BPs leads to altered gene expression, developmental abnormalities, impaired reproduction, and disrupted hormonal signalling in various fish species. Although BPA alternatives like bisphenol S, bisphenol F, or bisphenol AF were introduced as safer options, emerging evidence suggests they may pose equal or greater risks. Regulatory measures are evolving, particularly within the European Union, but legislation remains limited for many bisphenol analogues. This review emphasises the need for comprehensive environmental monitoring, stricter regulatory frameworks, and the development of genuinely safer alternatives to minimise the ecological and health impacts of BPs in aquatic systems. Full article

Animal venoms are complex biochemical secretions rich in highly potent and selective bioactive molecules, including peptides, enzymes, and small organic compounds. Once associated primarily with toxicity, these venoms are now recognized as a promising source of therapeutic agents for a wide range of medical conditions. This review provides a comprehensive analysis of the pharmacological potential of venom-derived compounds, highlighting their mechanisms of action, such as ion channel modulation, receptor targeting, and enzyme inhibition. Successful venom-derived drugs like captopril and ziconotide exemplify the translational potential of this biological arsenal. We discuss therapeutic applications in cardiovascular diseases, chronic pain, cancer, thrombosis, and infectious diseases, as well as emerging peptide candidates in clinical development. Technological advancements in omics, structural biology, and synthetic peptide engineering have significantly enhanced the discovery and optimization of venom-based therapeutics. Despite challenges related to stability, immunogenicity, and ecological sustainability, the integration of AI-driven drug discovery and personalized medicine is expected to accelerate progress in this field. By synthesizing current findings and future directions, this review underscores the transformative potential of animal venoms in modern pharmacotherapy and drug development. We also discuss current therapeutic limitations and how venom-derived compounds may address unmet needs in specific disorders. Full article

Within the framework of organic acid alternatives to chemical herbicides, pre-emergence weed control research is scarce. Citric acid (CA) and lactic acid (LA), considered significantly less effective than pelargonic acid (PA) and acetic acid (AA) from post-emergence (foliar spraying) studies, have largely been disregarded. This in vitro study was aimed at comparing the effects of 5–20% AA, AA + essential oils, PA, CA, and LA on radicle emergence inhibition (direct spraying of seeds) and shoot emergence inhibition (application to peat) on both weeds (perennial ryegrass, green foxtail, common vetch and chicory) and crops (soft wheat, alfalfa and millet). All tested compounds demonstrated concentration-dependent and species-specific effects on shoot emergence inhibition, with CA and LA (IC₅₀ range: 3.4–19.3%) showing a comparable efficacy to PA and AA (IC₅₀ range: 3.1–35.9%). The results also showed that CA and, to a lesser extent, LA were less inhibitory to soft wheat (CA IC₅₀ = 62.5%; LA IC₅₀ = 35.9%) and alfalfa (CA IC₅₀ = 57.8%; LA IC₅₀ = 44.1%) shoot emergence. CA and LA show potential promise for pre-emergence weed control in field testing, either on a stale seedbed in pre-crop sowing or concurrently with soft wheat and alfalfa sowing. Investigating organic compound herbicidal effects on crops of interest warrants attention. Full article

As sessile organisms, plants adapt to environmental challenges through flexible developmental and physiological programs. Hormones play a central role in this adaptability, integrating environmental signals into coordinated responses that regulate growth and stress tolerance. Comparative studies across photosynthetic lineages reveal that several core hormone functions are remarkably conserved, despite major evolutionary changes in hormone perception, biosynthesis, metabolism, and transport. This conservation suggests that plant hormones have played a pivotal evolutionary role—not only preserving essential biological functions but also enabling increased complexity in plant form and function. A similar dual role is observed in evolutionary endocrinology in animals, where hormones contribute to the emergence and regulation of complex traits. We propose that hormones such as cytokinins, auxins, brassinosteroids, strigolactones, and abscisic acid originated as metabolic derivatives closely tied to core physiological functions essential for survival and reproduction, including reproductive success, nutrient sensing, and dehydration tolerance. Over time, these compounds were progressively integrated into increasingly sophisticated regulatory networks, where they now serve as central coordinators and key targets of evolutionary selection. This model advances our understanding of hormone evolution by providing a structured framework to interpret the persistence, specialization, and integration of plant hormones across evolutionary timescales. Full article

With the ongoing advancement of industrialization and rapid urbanization, the emission of volatile organic compounds (VOCs) has increased significantly. As key precursors of PM_2.5 and ozone formation, VOCs pose a growing threat to the health of ecosystems. Due to their complex and dynamic transformation processes across air, water, and soil media, the ecological risks associated with VOCs have attracted increasing attention from both the scientific community and policy-makers. This study systematically reviews the core literature on the ecological impacts of VOCs published between 2005 and 2024, based on data from the Web of Science and Google Scholar databases. Utilizing three bibliometric tools (CiteSpace, VOSviewer, and Bibliometrix), we conducted a comprehensive visual analysis, constructing knowledge maps from multiple perspectives, including research trends, international collaboration, keyword evolution, and author–institution co-occurrence networks. The results reveal a rapid growth in the ecological impact of VOCs (EIVOCs), with an average annual increase exceeding 11% since 2013. Key research themes include source apportionment of air pollutants, ecotoxicological effects, biological response mechanisms, and health risk assessment. China, the United States, and Germany have emerged as leading contributors in this field, with China showing a remarkable surge in research activity in recent years. Keyword co-occurrence and burst analyses highlight “air pollution”, “exposure”, “health”, and “source apportionment” as major research hotspots. However, challenges remain in areas such as ecosystem functional responses, the integration of multimedia pollution pathways, and interdisciplinary coordination mechanisms. There is an urgent need to enhance monitoring technology integration, develop robust ecological risk assessment frameworks, and improve predictive modeling capabilities under climate change scenarios. This study provides scientific insights and theoretical support for the development of future environmental protection policies and comprehensive VOCs management strategies. Full article

The dual environmental challenges of karst areas lie in organic solid waste’s (OSW) massive generation scale and diffuse dispersion, which accelerate bedrock exposure and soil contamination, while simultaneously representing an underutilized resource for soil amendments through optimized composting. Bio-enhanced composting of multi-source OSW yields compounds with dual redox/adsorption capabilities, effectively improving soil quality and restoring ecological balance. The recycling and circular utilization of OSW resources become particularly critical in karst regions with vulnerable soil ecosystems, where sustainable resource management is urgently needed to maintain ecological balance. This review elucidates the ecological impacts of multi-source OSW compost applications on soil environments in ecologically fragile karst regions, specifically elucidating the mechanisms of heavy metals (HMs) migration–transformation and organic contaminant degradation (with emphasis on emerging pollutants), and the functional role of microbial carbon pumps in these processes. Furthermore, establishing a sustainable “multi-source OSW−compost−organic matter (adsorption and redox sites)−microorganisms−pollution remediation” cycle creates a green, low-carbon microenvironment for long-term soil remediation. Finally, this study evaluates the application prospects of the refined composting technology utilizing multi-objective regulation for OSW resource recycling and utilization in karst areas. This review provides critical insights for optimizing soil remediation strategies in karst ecosystems through organic waste valorization. Full article