Search Results (331)

In recent decades, fishery stock enhancement has been increasingly utilized as a restoration tool to mitigate population declines and enhance the resilience of marine fisheries. Nevertheless, persistent enhancement efforts risk eroding the evolutionary potential of wild populations via genetic homogenization and maladaptive gene flow. Using long-term monitoring data (2017–2023), we quantified the effects of large-scale Penaeus penicillatus stock enhancement (~10⁸ juveniles/yr) on wild population dynamics and genetic integrity in the Beibu Gulf ecosystem. Temporal genetic changes were assessed using eight highly polymorphic microsatellite loci, comparing founder (2017) and enhanced (2024) populations to quantify stocking impacts. Insignificantly lower expected heterozygosity was observed in the stocked population (He = 0.60, 2024) relative to natural populations (He = 0.62–0.66; p > 0.1), indicating genetic dilution effects from enhancement activities. No significant erosion of genetic diversity was detected post-enhancement, suggesting current stocking practices maintain short-term population genetic integrity. Despite conserved heterozygosity, pairwise Fst analysis detected significant genetic shifts between temporal cohorts (pre-enhancement—2017 vs. post-enhancement—2024; Fst = 0.25, p < 0.05), demonstrating stocking-induced population restructuring. Genetic connectivity analysis revealed that while the enhanced Beihai population (A-BH) maintained predominant self-recruitment (>90%), it experienced substantial stocking-derived gene flow (17% SW → A-BH). The post-stocking period showed both reduced genetic exchange with adjacent populations and increased asymmetric dispersal from A-BH (e.g., 5% to YJ), indicating that hatchery releases simultaneously enhanced population isolation while altering regional genetic structure. Our findings revealed the paradoxical dual effects of stock enhancement and allelic diversity while disrupting natural genetic architecture. This underscores the need for evolutionary-impact assessments in marine resource management. Full article

CD8+ T lymphocytes (CTLs) act as serial killers of infected or malignant cells by releasing large amounts of interferon-gamma (IFNγ) and granzymes. Although IFNγ is a pleiotropic cytokine with diverse immunomodulatory functions, its precise spatiotemporal regulation and role in CTL-mediated cytotoxicity remain incompletely understood. Using wild-type and granzyme B-mTFP knock-in mice, we employed a combination of in vitro approaches, including T cell isolation and culture, plate-bound anti-CD3e stimulation, degranulation assays, flow cytometry, immunofluorescence, and structured illumination microscopy, to investigate IFNγ dynamics in CTLs. IFNγ expression in CTLs was rapid, transient, and strictly dependent on T cell receptor (TCR) activation. We identified two functionally distinct IFNγ-producing subsets: IFNγ^high (IFNγ^hi) and IFNγ^low (IFNγ^lo) CTLs. IFNγ^hi CTLs exhibited an effector/effector memory phenotype, significantly elevated CD107a surface expression (a marker of lytic granule exocytosis), and higher colocalization with cis-Golgi and granzyme B compared to IFNγ^lo CTLs. Furthermore, CRTAM, an early activation marker, correlated with IFNγ expression in naive CTLs. Our findings establish a link between elevated IFNγ production and enhanced CTL cytotoxicity, implicating CRTAM as a potential regulator of early CTL activation and IFNγ induction. These insights provide a foundation for optimizing T cell-based immunotherapies against infections and cancers. Full article

Fuel electro-hydraulic servo valves are core components in the fuel control system of aero-engines, and their performance directly affects thrust regulation and power output precision. Due to the combustibility of the working medium in fuel systems and the lack of effective circulation filtration, the retention of micron-sized particles within the valve gap can lead to valve spool jamming, which is a critical reliability issue. This study, based on fractal theory and the liquid–solid two-phase flow model, proposes a parametric model for non-ideal surface valve gaps and analyzes the dynamics of particles subjected to drag, lift, and buoyant forces on rough surfaces. By numerically analyzing flow field models with different roughness levels and comparing them with an ideal smooth gap model, the migration characteristics of particles were studied. To verify the accuracy of the model, an upscaled experimental setup was built based on similarity theory, and PIV experiments were conducted for validation. Experimental results show that the particle release position and valve surface roughness significantly affect particle migration time. The weight of the release position on particle migration time is 63%, while the impact of valve surface roughness is 37%. In models with different roughness levels, the particle migration time increases more rapidly for roughness values greater than Ra0.4, while for values less than Ra0.4, the increase in migration time is slower. Furthermore, the study reveals that particle migration trajectories are independent of flow velocity, with velocity only affecting particle migration time. This research provides theoretical support for enhancing the reliability of fuel electro-hydraulic servo valves and offers a new perspective for the design of highly reliable hydraulic components. Full article

Investigating avalanche hazards is a fundamental preliminary task in avalanche research. This work is critically important for establishing avalanche warning systems and designing mitigation measures. Primary research data originated from field investigations and UAV aerial surveys, with avalanche counts and timing identified through image interpretation. Snowpack properties were primarily acquired via in situ field testing within the study area. Methodologically, statistical modeling and RAMMS::AVALANCHE simulations revealed spatiotemporal and dynamic characteristics of avalanches. Subsequent application of the Certainty Factor (CF) model and sensitivity analysis determined dominant controlling factors and quantified zonal influence intensity for each parameter. This study, utilizing field reconnaissance and drone aerial photography, identified 86 avalanche points in the study area. We used field tests and weather data to run the RAMMS::AVALANCHE model. Then, we categorized and summarized regional avalanche characteristics using both field surveys and simulation results. Furthermore, the Certainty Factor Model (CFM) and the parameter Sensitivity Index (Sa) were applied to assess the influence of elevation, slope gradient, aspect, and maximum snow depth on the severity of avalanche disasters. The results indicate the following: (1) Avalanches exhibit pronounced spatiotemporal concentration: temporally, they cluster between February and March and during 13:00–18:00 daily; spatially, they concentrate within the 2100–3000 m elevation zone. Chute-confined avalanches dominate the region, comprising 73.26% of total events; most chute-confined avalanches feature multiple release areas; therefore the number of release areas exceeds avalanche points; in terms of scale, medium-to-large-scale avalanches dominate, accounting for 86.5% of total avalanches. (2) RAMMS::AVALANCHE simulations yielded the following maximum values for the region: flow height = 15.43 m, flow velocity = 47.6 m/s, flow pressure = 679.79 kPa, and deposition height = 10.3 m. Compared to chute-confined avalanches, unconfined slope avalanches exhibit higher flow velocities and pressures, posing greater hazard potential. (3) The Certainty Factor Model and Sensitivity Index identify elevation, slope gradient, and maximum snow depth as the key drivers of avalanches in the study area. Their relative impact ranks as follows: maximum snow depth > elevation > slope gradient > aspect. The sensitivity index values were 1.536, 1.476, 1.362, and 0.996, respectively. The findings of this study provide a scientific basis for further research on avalanche hazards, the development of avalanche warning systems, and the design of avalanche mitigation projects in the study area. Full article

The present study focuses on the dynamics of trace metals (TM) in two European rivers, the Mosel and the Meuse. A deterministic description of hydro-sedimentary processes has been performed. The model used to describe pollutant transport and dilution at the watershed scale has been enhanced with the implementation of the MicMod sub-model. The objective of this study is to characterise the dynamics of TM in the water column and bed sediment. A multi-class grain size representation has been developed in MicMod. The dissolved and particulate TM phases have been calculated with specific partitioning coefficients associated with each suspended sediment (SS) class. The processes involved in TM fate have been calibrated in MicMod, including settling velocity, TM releases from the watershed (point and diffuse loads), etc. Following the calibration of the parameters involved in TM transport within the river ecosystem, the main goal is to describe TM dynamics using a pressure–impact relationship model. It was demonstrated that the description of at least one class of fine particles is necessary to obtain an adequate representation of TM concentrations. The focus of this study is low flow periods, which are characterised by the presence of fine particles. The objective is to gain a deeper understanding of the processes that control the transport of TM. This paper establishes consistent pressure–impact relationships between TM loads (urban, industrial, soils) from watersheds and concentrations in rivers. Full article

This paper presents a detailed analysis of the dynamics of indoor environmental parameters under three simulated fire scenarios in a multi-story building, using the PyroSim platform (based on the Fire Dynamics Simulator—FDS). The study compares two smoke control strategies, organized natural ventilation (a passive system) and mechanical pressurization (an active system), evaluating their influence on temperature, differential pressure, air velocity, heat release rate (HRR), and toxic gas distribution. The simulations revealed that passive systems, relying on the stack effect and vertical natural ventilation, do not ensure the effective control of smoke infiltration into evacuation routes, allowing significant heat accumulation and reduced visibility. The results highlight the superior effectiveness of unidirectional mechanical pressurization in maintaining a stable flow regime, functional visibility, and a safe evacuation environment. A key finding is the transition from static pressure control to velocity-based flow control at the moment of door opening toward the fire source. The results confirm that a dynamically adapted application of mechanical pressurization—synchronized with the opening of access pathways—not only reinforces existing principles for protecting egress routes, but also provides a precise operational approach for optimizing emergency responses in high-rise buildings. Full article

A technique has been proposed and experimentally tested for measuring the hydrogen concentration in an inert atmosphere within a closed system. This method utilizes a metal-oxide-semiconductor field-effect capacity-type (MOSFEC) sensor under harsh conditions such as exposure to inert gases, pressure fluctuations, and varying temperatures. The measurement is performed during the thermal decomposition of metal hydrides in a liquid sodium environment. The developed measurement technique for determining hydrogen concentration released from metal hydride samples in a system with a closed gas path is cost-effective compared to standardized, resource-intensive open-volume flow measurement methods. The use of the developed MOSFEC sensor technique allows for rapid and efficient investigation of the in situ real-time dynamics of gas release from various metal hydride materials differing in their hydrogen content within a small closed volume. Additionally, this approach enables precise determination of the specific gas release temperatures. Full article

Polyomaviruses have the potential to cause significant morbidity not only in transplant medicine, but also in other forms of disease or variants of immunosuppression. In kidney transplant recipients or recipients of human stem cell transplants, the BK-Virus is the major proponent of manifestations such as BKPyV-associated nephropathy or hemorrhagic cystitis. As no polyomavirus-specific drug with proven in vivo effects has been developed so far, methods to screen for such drugs are important. This work describes the establishment of a virus-secreting cell line. By infecting a pre-established monkey kidney cell line (COS-1) with a non-rearranged human BK polyomavirus isolated from a kidney transplant patient suffering from BKPyV-associated nephropathy, a continuously replicating cell type with consistent virus secretion could be established and was termed COSSA. Measurements of BKPyV replication, virion production, and secretion were performed both intracellularly and in the cell supernatant. Viral proteins such as VP1 and LTAg were accurately tracked by confocal microscopy, as well as by immunoblot and qPCR. An intracellular flow cytometry (FACS) assay detecting VP1 protein was established and revealed an expanded range of positive intracellular signals. The viruses produced proved to be infectious in human tubular epithelial cell lines. Long-range sequencing of the COSSA genome using Oxford Nanopore Technology revealed a total of five distinct BKPyV integration events. One integration of a partial BKPyV genome was located upstream of the epidermal growth factor receptor gene. The second and third, both truncated forms of integration, were close to histocompatibility gene locuses, while the fourth was characterized by a ninefold and the fifth by a fourfold tandem repeat of the BKPyV genome. From both of the repeat forms, virus replicates were derived showing deletions/duplications on early and late genes and inversions within the non-coding control region (NCCR). This pattern of repetitive viral genome integration is a potential key driver of enhanced viral replication and increased virion assembly, ultimately supporting efficient virus egress. Quantitative PCR analysis confirmed the release of approximately 10⁸/mL viral units per 48 h from 2 × 10⁵ COSSA cells into the culture supernatant. Notably, the NCCR region of the most frequent copies of circular virus and the integrated tetrameric tandem repeat exhibited a rearranged configuration, which may contribute to the observed high replication dynamics. The establishment of a consistent methodology to generate and secrete BKPyV from a cell line is expected to significantly facilitate antiviral drug development. Full article

Phosphorus flame retardants react with cellulose hydroxyl groups via esterification, enhancing the effectiveness of char formation, which is beneficial in terms of the search for bio-sourced flame retardants. The current work assessed the flammability of a new polymer synthesized by Michael 1,4-addition (rP) and modified with developed intumescent flame retardant systems (FRs), in which lignocellulose components, such as sunflower husk (SH) and peanut shells (PS), replaced a part of the synthetic ones. The thermal and thermomechanical properties of the rP, with 20 wt.% each from six FRs, were determined by thermogravimetric analysis (TG), differential scanning calorimetry (DSC) and dynamic mechanical thermal analysis (DMTA). Moreover, the flammability and evolved gas were studied with pyrolysis combustion flow calorimetry (PCFC) and thermogravimetric analysis connected with Fourier transform infrared spectroscopy tests (TGA/FT-IR). The effects were compared to those achieved for unmodified rP and a polymer with a commercially available intumescent flame retardant (IFR). The notable improvement, especially in terms of the heat release rate and heat release capacity, indicates that the system with melamine phosphate (MP) and peanut shells (PS) can be used to decrease the flammability of new polymers. An extensive analysis of the composition and geometry of the ground shells and husk particles preceded the research. Full article

This study examines the cytotoxicity of two silver nanoparticle formulations—AgNPs conjugated with chlorhexidine (AgNPs-CHL) and AgNPs conjugated with polyethylene glycol and metronidazole (AgNPs-PEG-MET)—as examples of the surface functionalization of silver nanoparticles with drugs via sulfur–silver bonds and nitrogen–silver interactions. We previously reported the synthesis of these NPs and their efficiency in periodontitis treatment. Here, we analyze the relationships between the cytotoxic mechanisms of AgNPs and their surface chemistry. UV–Vis spectroscopy, dynamic light scattering (DLS), and scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) were used for physicochemical studies of the conjugates in two environments: aqueous solutions and commonly used cell culture media. Cytotoxicity was assessed in human fetal osteoblasts (hFOB 1.19) and human gingival fibroblasts (HGF-1) through BrdU and LDH assays, ROS detection, cell cycle analysis, apoptosis assays, and protein expression studies. AgNPs-CHL showed aggregation and increased hydrodynamic diameters in the culture medium, while AgNPs-PEG-MET remained stable. Both exhibited concentration-dependent cytotoxicity: AgNPs-CHL at 0.4–10 μg/mL and AgNPs-PEG-MET at 0.75–10 μg/mL. AgNPs-CHL, in which silver surface functionalization was realized via nitrogen–silver interactions, induced significant ROS generation, LDH release, and necroptosis, marked by increased RIP1, RIP3, and MLKL proteins. In the case of AgNPs-PEG-MET, where sulfur–silver bonds combined the drug via a PEG linker, they triggered apoptosis, as evidenced by elevated caspase-2 levels and flow cytometry. These findings highlight that the type of surface functionalization of silver nanoparticles significantly influences their physicochemical behavior and biological effects. Understanding these mechanisms is crucial in designing safer, more effective nanoparticle-based therapies for periodontal and other inflammatory conditions. Full article

The safety performance of liquid hydrogen storage has a significant influence on its large-scale commercial application. Due to the complexity and costs of experimental investigation, computational fluid dynamics (CFD) simulations have been extensively applied to investigate the dynamic behaviors of liquid hydrogen release. The involved physical and chemical models, such as models of species thermodynamic properties and liquid–gas mass transfer, play a major role for the entire CFD model performance. However, comprehensive investigations into their impacts remain insufficient. In this study, CFD models of liquid hydrogen release were developed by using two widely used commercial simulation tools, Fluent and FLACS, and validated against experimental data available in the literature. Comparisons of the model results reveal strong discrepancies in the prediction accuracy of temperature and hydrogen volume fraction between the two models. The impact of the models of thermodynamic properties and liquid–gas mass transfer on the prediction results was subsequently explored by incorporating the FLACS sub-models to Fluent and evaluating the resulting prediction differences in temperatures and hydrogen volume fractions. The results show that the models of thermodynamic properties and liquid–gas mass transfer used in FLACS underestimate the vertical rise height and the highest hydrogen volume fraction of the cloud. Sensitivity analyses on the parameters in these sub-models indicate that the specific heats of hydrogen and nitrogen, in conjunction with the mass flow rate and outflow density of the mass transfer model, have a significant influence on model prediction of temperature. Full article

This experimental study examines the particle-level combustion behavior of high-volatile bituminous coal and walnut shell particles in oxyfuel environments, with a particular focus on the gas-phase ignition characteristics and the structural development of volatile flames. Particles with similar size and shape distributions (a median diameter of about 126 µm and an aspect ratio of around 1.5) are combusted in hot flows generated using lean, flat flames, where the oxygen mole fraction is systematically varied in both CO₂/O₂ and N₂/O₂ atmospheres while maintaining comparable gas temperatures and particle heating rates. The investigation employs a high-speed multi-camera diagnostic system combining laser-induced fluorescence of OH, diffuse backlight-illumination, and Mie scattering to simultaneously measure the particle size, shape, and velocity; the ignition delay time; and the volatile flame dynamics during early-stage volatile combustion. Advanced detection algorithms enable the extraction of these multiple parameters from spatiotemporally synchronized measurements. The results reveal that the ignition delay time decreases with an increasing oxygen mole fraction up to 30 vol%, beyond which point further oxygen enrichment no longer accelerates the ignition, as the process becomes limited by the volatile release rate. In contrast, the reactivity of volatile flames shows continuous enhancement with an increasing oxygen mole fraction, indicating non-premixed flame behavior governed by the diffusion of oxygen toward the particles. The analysis of the flame stand-off distance demonstrates that volatile flames burn closer to the particles at higher oxygen mole fractions, consistent with the expected scaling of O₂ diffusion with its partial pressure. Notably, walnut shell and coal particles exhibit remarkably similar ignition delay times, volatile flame sizes, and OH-LIF intensities. The substitution of N₂ with CO₂ produces minimal differences, suggesting that for 126 µm particles under high-heating-rate conditions, the relatively small variations in the heat capacity and O₂ diffusivity between these diluents have negligible effects on the homogeneous combustion phenomena observed. Full article

With the large-scale grid connection of renewable energy and the surge of peak power system demand, liquid carbon dioxide energy storage technology has become a research hotspot due to its high energy density and environmental friendliness. However, most of the existing research focuses on the steady-state performance of the system, and the parameter coupling and transient response characteristics under dynamic operating conditions are not yet clear. To this end, this paper constructs a dynamic simulation model of a 10 MW-class liquid carbon dioxide energy storage (LCES) based on the Simulink platform, focuses on the coupling effects of the compressor inlet temperature, pressure, and mass flow rate and the expander inlet mass flow rate on the system parameters, and reveals the dynamic correlation between the system work and the state of charge value of the tank under the variable power working condition. The results show that the system’s round-trip efficiency (RTE) is 65.3% under design conditions, and the energy density reaches 34.79 kW·h·m⁻³. Perturbation analysis shows that when the compressor inlet temperature rises from 283.15 K to 303.15 K, the power consumption fluctuates in the range of 96.84% to 102.99% under design conditions. The inlet pressure perturbation (0.5~1.5 bar) will cause the power consumption of the compressor to change by 80.2%. In variable power operation, the state of charge value of the high-pressure liquid tank level in the energy storage stage rises from 0 to 84.89%, and the state of charge value of the high-pressure liquid tank level in the energy release stage decreases from 84.89% to 31.48%. The dynamic model proposed in this paper can accurately capture the transient response characteristics of the system and provide theoretical support for the optimization design and engineering application of LCES. Full article

Small bookstores constructed before the 1970s have a high fire risk in the context of the revitalization of historical buildings; while the setup of simple sprinklers is an effective and cheap method of extinguishing fires, the parameters of the sprinklers are uncertain. In this study, small bookstores in Beijing were selected, and physical combustion experiments with/without a sprinkler system were carried out following the provisions of the Code for the Design of Sprinkler Systems. After the experiments, an FDS model was set up using fire dynamics software. The results show that the total heat release rate (HRR) of books and desks is related to the square of time, with a coefficient of 2.528 × 10⁻⁶, and the maximum heat release rate is 40 KW. Unlike the standard test, the physical combustion experiment is significantly affected by the space. According to numerical simulations, when the sprinkler flow velocity is 60~100 L/min, the water consumption of the sprinkler is 195~218 L. This study lays the foundation for the analysis of the combustion characteristics of small bookstores and provides data support for the installation of simple sprinkler systems in small bookstores. Full article

LDPE was crosslinked with novel dynamic or conventional crosslinking agents during melt processing. Both crosslinkers were synthesized by the esterification of Thiele’s acid or adipic acid with 4-hydroxy-TEMPO. ¹H-NMR showed that a temperature of 170 °C and a reaction time of 24 min are required for a successful crosslinking. The concentrations of crosslinking agents were 1.45, 2.9, and 5.8 mol%. Conventionally crosslinked LDPEs show a decrease in soluble content in hot xylene with increased crosslinker concentrations, while dynamically crosslinked LDPEs show no change after thermal treatment, indicating the scission of dynamic crosslinks. The rheology of both crosslinked LDPEs at 130 °C shows that the stress release is slower than that of neat LDPE, confirming crosslinking, while at 170 °C a shift in the stress release and also a shift in the flow properties of dynamically crosslinked LDPE towards those of neat LDPE are observed, both indicating the cleavage of dynamic crosslinks. Compared to neat LDPE, the mechanical properties of both crosslinked LDPEs show an increase in Young’s modulus and tensile strength and a decrease in elongation and creep when the concentration of both crosslinkers is increased. By increasing the processing temperature to 170 °C, the crystallinity index decreases, leading to a rather small improvement in the mechanical properties. Full article