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6 Results Found

  • Protocol
  • Open Access
5 Citations
13,298 Views
7 Pages

Coomassie brilliant blue (CBB) dyes have been commonly used for the staining of protein bands in polyacrylamide gel electrophoresis (PAGE) gels. However, the staining and destaining of CBB dyes are time-consuming, and the use of methanol is hazardous...

  • Protocol
  • Open Access
30 Citations
13,980 Views
15 Pages

A Method to Reuse Archived H&E Stained Histology Slides for a Multiplex Protein Biomarker Analysis

  • James P. Hinton,
  • Katerina Dvorak,
  • Esteban Roberts,
  • Wendy J. French,
  • Jon C. Grubbs,
  • Anne E. Cress,
  • Hina A. Tiwari and
  • Raymond B. Nagle

15 November 2019

Archived Hematoxylin and Eosin (H&E) stained pathology slides are routinely stored to index formalin-fixed paraffin-embedded (FFPE) sample tissue blocks. FFPE blocks are clinically annotated human tumor specimens that can be valuable in studies d...

  • Article
  • Open Access
33 Citations
11,798 Views
17 Pages

Isolation, Screening, and Identification of Alkaline Protease-Producing Bacteria and Application of the Most Potent Enzyme from Bacillus sp. Mar64

  • Essam Kotb,
  • Amira H. Alabdalall,
  • Mariam A. Alsayed,
  • Azzah I. Alghamdi,
  • Eida Alkhaldi,
  • Sayed AbdulAzeez and
  • J. Francis Borgio

In this study, thirty-seven alkaline protease-producing bacteria were recovered from different regions of Saudi Arabia. The proteolytic strain with the highest productivity was identified as Bacillus sp. Mar64. Maximum productivity of Mar64P alkaline...

  • Article
  • Open Access
2 Citations
1,853 Views
14 Pages

8 November 2024

In recent years, graphene oxide (GO)-based two-dimensional (2D) laminar membranes have attracted considerable attention because of their unique well-defined nanochannels and deliver a wide range of molecular separation properties and fundamentals. Ho...

  • Article
  • Open Access
5 Citations
1,807 Views
15 Pages

Modulation of Interlayer Nanochannels via the Moderate Heat Treatment of Graphene Oxide Membranes

  • Na Meng,
  • Xin Sun,
  • Jinxin Liu,
  • Jialing Mi,
  • Xuan Chen and
  • Rong Rong

2 August 2024

In response to the phenomenon of interlayer transport channel swelling caused by the hydration of oxygen-containing functional groups on the GO membrane surface, a moderate heat treatment method was employed to controllably reduce the graphene oxide...

  • Article
  • Open Access
8 Citations
3,018 Views
12 Pages

The Effects of Staining and Cleaning on the Color and Light Transmittance Changes of a Copolyester Retainer Material with Different Surface Textures

  • Laurie Susarchick,
  • Insia Virji,
  • Grace Viana,
  • Mervat Mahmoud,
  • Veerasathpurush Allareddy,
  • Max Gruber,
  • Henry Lukic,
  • Spiro Megremis and
  • Phimon Atsawasuwan

30 September 2022

This study evaluated the efficacy of different cleaning methods with respect to changes in the color and light transmittance of both rough and smooth thermoformed, copolyester retainer specimens, after staining in different solutions. Four hundred co...