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Rapid Electrophoretic Staining and Destaining of Polyacrylamide Gels

by Fumihiro Motojima 1,2,†
1
Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan
2
Department of Molecular Bioscience, Kyoto Sangyo University, Kamigamo-Motoyama, Kyoto 603-8555, Japan
Current address: Fuji Chemical Industries Co., Ltd., 1 Gohkakizawa, Kamiichi-machi, Nakaniikawa-gun, Toyama 930-0397, Japan
Methods Protoc. 2018, 1(2), 13; https://doi.org/10.3390/mps1020013
Received: 6 February 2018 / Revised: 22 March 2018 / Accepted: 29 March 2018 / Published: 10 April 2018
Coomassie brilliant blue (CBB) dyes have been commonly used for the staining of protein bands in polyacrylamide gel electrophoresis (PAGE) gels. However, the staining and destaining of CBB dyes are time-consuming, and the use of methanol is hazardous to one’s health. I introduce a rapid electrophoretic destaining method using a semi-dry transfer unit and a high current power supply. In this method, ethanol was used instead of the hazardous methanol. Most of the protein bands became visible in 30 min. After a secondary destaining step, residual CBB was completely destained. The detection limit for a tested protein (5 ng) was higher than that of the conventional method. Therefore, this method is superior in its speed, safety, low cost, and sensitivity. View Full-Text
Keywords: SDS-PAGE; Coomassie brilliant blue; electrophoresis; staining; destaining SDS-PAGE; Coomassie brilliant blue; electrophoresis; staining; destaining
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Motojima, F. Rapid Electrophoretic Staining and Destaining of Polyacrylamide Gels. Methods Protoc. 2018, 1, 13.

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