Search Results (521)

Furunculosis caused by Aeromonas salmonicida poses a significant challenge to the sustainable production of maraena whitefish (Coregonus maraena). This case report outlines a multi-year disease management strategy at a European whitefish facility with two production departments, each specialising in different life-cycle stages. Recurrent outbreaks of A. salmonicida necessitated the development of effective vaccination protocols. Herd-specific immersion vaccines failed to confer protection, while injectable formulations with plant-based adjuvants caused severe adverse reactions and mortality rates exceeding 30%. In contrast, the bivalent vaccine Alpha Ject 3000, containing inactivated A. salmonicida and Vibrio anguillarum with a mineral oil adjuvant, yielded high tolerability and durable protection in over one million whitefish. Post-vaccination mortality remained low (3.3%), aligning with industry benchmarks, and furunculosis-related losses were fully prevented in both departments. Transcriptomic profiling of immune-relevant tissues revealed distinct gene expression signatures depending on vaccine type and time post-vaccination. Both the herd-specific vaccine and Alpha Ject 3000 induced the expression of immunoglobulin and inflammatory markers in the spleen, contrasted by reduced immunoglobulin transcript levels in the gills and head kidney together with the downregulated expression of B-cell markers. These results demonstrate that an optimised injectable vaccination strategy can significantly improve health outcomes and disease resilience in maraena whitefish aquaculture. Full article

The current proceedings summarize the presentations delivered during the third International Electronic Conference on Microbiology (ECM 2025), which was held online from 1 to 3 April 2025, via the SciForum platform. This virtual event brought together researchers from around the world to share recent advances in microbiological sciences. The ECM 2025 highlighted recent developments across a broad spectrum of microbiological research, including antimicrobial resistance, gut microbiota, infectious diseases, and environmental microbiomes. Participants shared their work through online presentations and abstracts, with selected submissions invited for full publication. The event fostered global collaboration, promoted open-access science, and showcased innovative tools for studying and managing microbial systems in health, agriculture, and industry. The multidisciplinary program was organized into several thematic sessions: S1. Gut Microbiota and Health Disease. S2. Foodborne Pathogens and Food Safety. S3. Antimicrobial Agents and Resistance. S4. Emerging Infectious Diseases. S5. Microbiome and Soil Science. S6. Microbial Characterization and Bioprocess. S7. Microbe–Plant Interactions. This conference report presents summaries of the contributions made by participating authors over the three-day event. Full article

Traditional type I sourdoughs are being rediscovered and increasingly used in artisanal and industrial bakeries due to the unique taste and texture, potential health benefits, and longer shelf life they confer on to baked products. These unique properties are attributed to the diverse microbial communities of sourdough, comprising both yeasts and bacteria. The traditional preservation method for type I sourdough (i.e., continuous backslopping) may lead, over time, to taxonomic and functional rearrangements of its microbial communities. Consequently, significant deviations in the characteristics of baked products can occur. In this context, this review aims to summarize the recent literature on the long-term preservation and maintenance strategies for type I sourdough and highlight the essential role that microbial biological resource centers (mBRCs) could play in the preservation and sharing of sourdough microbiomes. Specifically, the identification of appropriate preservation methods, implementation of well-defined access and benefit-sharing protocols, and development of microbiome-specific datasets, should be encouraged within the context of mBRCs. These infrastructures are expected to play a pivotal role in preserving the microbiota of fermented foods, serving as a crucial element for innovation and the safeguarding of traditional foods and culinary heritage. Full article

In recent years, the number of fake news stories has significantly increased in the world of media, especially with the widespread use of social media. It has impacted several industries, including tourism. From a tourism point of view, the spread of fake news can contribute to the reduction of the popularity of a destination. It may influence travel decisions by discouraging tourists from visiting certain places and thus damage the reputation of the destination, contributing to economic loss. After a literature review on the communication aspect of fake news and a general introduction of fake news in the tourism and hospitality industry, we conducted a systematic literature review (SLR), a research methodology to collect, identify, and analyse available research studies through a systematic procedure. The current SLR is based on the Scopus, Web of Science, and Google Scholar databases of existing literature on the topic of fake news in the tourism and hospitality industry. The study identifies, lists, and examines existing papers and conference proceedings from a vast array of disciplines, in order to give a well-rounded view on the issue of fake news in the tourism and hospitality industry. After selecting a total of 54 previous studies from more than 20 thousand results for the keywords ‘fake news’ and ‘tourism,’ we have analysed 39 papers in total. The SLR aimed to highlight existing gaps in the literature and areas that may require further exploration in future primary research. We have found that there is relatively limited academic literature available on the subject of fake news affecting tourism destinations, compared to studies focused on hospitality services. Full article

The construction industry is moving towards the era of industry 4.0; 5.0 with Building Information Modelling (BIM) as the tool gaining significant traction owing to its inherent advantages such as enhancing construction design, process and data management. However, the integration of BIM presents risks that are often overlooked in project implementation. This study aims to develop a novel amalgamated dimensional factor (Techno-organizational Aspect) that is set out to identify and align appropriate management strategies to these risks. Firstly, it encompasses an in-depth analysis of BIM and risk management, through an integrative review approach. The study utilizes an exploratory-based review centered around journal articles and conference papers sourced from Scopus and Google Scholar. Then processed using NVivo 12 Pro software to categorise risks through thematic analysis, resulting in a comprehensive Risk Breakdown Structure (RBS). Then qualitative content analysis was employed to identify and develop management strategies. Further data collection via online survey was crucial for closing the research gap identified. The analysis by mixed method research enabled to determine the risk severity via the quantitative approach using SPSS (version 29), while the qualitative approach linked management strategies to the risk factors. The findings accentuate the crucial linkages of key strategies such as version control system that controls BIM data repository transactions to mitigate challenges controlling transactions in multi-model collaborative environment. The study extends into underexplored amalgamated domains (techno-organisational spectrum). Therefore, a significant contribution to bridging the existing research gap in understanding the intricate relationship between BIM implementation risks and effective management strategies. Full article

Buckwheat, a dicotyledonous pseudocereal from the Polygonaceae family, has emerged as a crop of scientific and industrial interest due to its exceptional phytochemical profile, adaptability to different environments, and minimal agronomic input requirements. This paper aims to highlight the proximate composition (carbohydrates, protein, dietary fiber, lipids, starch, vitamins, and minerals) of the buckwheat principal species, Fagopyrum esculentum Moench (common buckwheat) and Fagopyrum tataricum (L.) Gaertn (Tartary buckwheat). Other bioactive compounds, including flavonoids (e.g., rutin, quercetin), phenolic acids, and anthocyanins, were emphasized, together with their influence on human health. These constituents confer a broad range of biological activities such as anti-inflammatory, antimicrobial, antidiabetic, antihypertensive, and hypoglycemic effects. Moreover, buckwheat is inherently gluten-free, making it a valuable alternative in formulations targeting gluten-sensitive populations. Finally, the review addresses the possibility of using starch buckwheat as a raw material in starch-based films. Further research is needed to elucidate the potential of buckwheat starch as a viable material for the development of biodegradable food packaging films. Full article

As global energy production progressively turns toward a green environment and economy, one of the safety challenges to the maritime industry that has arisen lies within offshore wind farms (OWFs). As renewable sources of energy whose numbers are rapidly expanding, their impact to the safety of navigation of the ships that navigate in their vicinity ought to be examined further. An ever-growing number of OWFs has led to safety concerns that have never been taken into consideration before. This article gives a structured quantitative analysis and an in-depth review of the literature connected to the safety of navigation, collision probability, and risk assessment that OWFs pose to all maritime industry agents. In this article, the main concerns of the impact of OWFs to the safety of navigation are analyzed using a combination of both the PRISMA and PICOC methodologies. Various types of scientific papers such as journal articles, conference proceedings, MSc theses, PhD theses, and online works of research are collated into a detailed bibliometric analysis and categorized by the most relevant parameters providing valuable perspectives on the current state of art in the field. The findings of this research emphasize the need for a further and more thorough analysis on the theoretical installment of OWFs and their inevitable impact on increasing maritime traffic complexity. The results of this article can form a strong basis for further scientific development in the field and can give useful insights to all maritime industry stakeholders dealing with OWFs. Full article

Accurate quantification of Bifidobacterium animalis subsp. lactis HN019, a clinically validated probiotic strain conferring immune modulation, gastrointestinal health, and gut barrier integrity benefits, is essential for diverse applications. To address the critical need for strain-specific detection, we developed a quantitative PCR (qPCR) assay targeting a unique single-nucleotide polymorphism (SNP) within the galK gene, identified through comparative whole-genome sequencing (WGS) analysis of 31 B. animalis subsp. lactis strains. The assay exhibited exceptional specificity, distinguishing HN019 from 19 other Bifidobacterium strains. Sensitivity tests indicated a detection limit of 0.5 pg of DNA and 10³ CFU/mL of bacterial cells, making it suitable for industrial-scale applications. Additionally, the method exhibited strong repeatability, reproducibility across different qPCR platforms, and resistance to interference from high cell density of B. animalis subsp. lactis DSMZ 10140. Successful quantification of HN019 in complex multi-strain probiotic powders confirmed its practical reliability. This work establishes a rapid, robust, and scalable tool for precise probiotic strain tracking, addressing critical quality control and regulatory compliance needs within the rapidly expanding probiotic industry. Full article

Housing retrofit plays a pivotal role in achieving sustainability goals. The fragmented nature of the retrofit industry has been identified as a barrier to driving retrofit at scale. The study aims to analyse how to strategically improve the concept of the one-stop-shop model to drive housing retrofit at scale with the help of existing literature. The concept of a one-stop-shop model provides all the retrofit services with a single contact to the client. A systematic literature review approach was used. Only peer-reviewed journal articles, book chapters and conference articles published from 2016 to 2025 in English were selected. There are 12 shortlisted journal and conference proceedings articles critically evaluated under three themes: delivery method, ownership structure and level of responsibility. The findings highlight the different characteristics of the one-stop-shop model under these themes. Considering the existing case studies, starting a one-stop shop under a hybrid delivery method and a medium level of responsibility is recommended for retrofit at scale. The ownership structure shall be context-specific. Limitations could be given as the researcher bias and the missed articles in databases not considered for this review. Further research is suggested on how the characteristics of a one-stop shop can be customised, considering the context-specific resources and purposes. Full article

Background/Objectives: More than 33 billion chickens are industrially raised for meat and egg production globally and vaccinated against Marek’s disease virus (MDV). The antigenically related herpesvirus of turkey (HVT) is used as a live-attenuated vaccine, commonly provided as a recombinant vector to protect chickens against additional unrelated pathogens. Because HVT replicates in a strictly cell-associated fashion to low levels of infectious units, adherent primary chicken or duck embryo fibroblasts are infected, dislodged from the cultivation surface and distributed as cryocultures in liquid nitrogen to the site of application. Although viable cells are complex products, application of infected cells in ovo confers protection even in presence of maternal antibodies. Methods/Results: The aim of our study was to determine whether a continuous cell line in a scalable cultivation format can be used for production of HVT-based vaccines. The AGE1.CR cell line (from Muscovy duck) was found to be highly permissive in adherent cultures. Propagation in suspension, however, initially gave very low yields. The induction of cell-to-cell contacts in carrier-independent suspensions and a metabolic shock improved titers to levels suitable for vaccine production (>10⁵ infectious units/mL after infection with multiplicity of 0.001). Conclusions: Production of HVT is challenging to scale to large volumes and the reliance on embryonated eggs from biosecure facilities is complex. We demonstrate that a cell-associated HVT vector can be propagated in a carrier-independent suspension culture of AGE1.CR cells in chemically defined medium. The fed-batch production is independent of primary cells and animal-derived material and can be scaled to large volumes. Full article

The Leontopodium R.Br. ex Cass. taxa have been extensively phytochemically researched for their beneficial properties by the pharmaceuticals industry. These species have been used as remedies since ancient times, and their usage in traditional medicine has been a source of inspiration for current research. As a result, a comprehensive review study concerning the bioactivities of secondary metabolites belonging to Leontopodium species would be of great interest. Our research shows that the majority of studies addressed the anti-inflammatory activity, and the most studied compound was leoligin, a lignan with cardioprotective properties. The diverse range of bioactivities were intricately linked to the abundance of secondary metabolites, which conferred effective antimicrobial activity, antioxidant properties, the anti-neurodegenerative potential due to improvements in cholinergic transmission, the anti-tumour effects on various cancer cell lines, particularly breast and lung cancer, and the hypoglycaemic and hepatoprotective properties. All these important bioactivities also recommend Leontopodium taxa as a valuable source for the discovery of new drugs. Full article

This systematic literature review examines the development of a conceptual certification framework for solar panel reuse, positioned within the broader context of the circular economy. It emphasizes sustainable production and consumption in response to the climate crisis and resource depletion. This review was conducted using Scopus and Google Scholar, following a structured search strategy. A final set of 63 sources, including peer-reviewed journal articles, conference papers, and gray literature recommended by domain experts, were selected to analyze existing certification frameworks across various sectors, focusing on their relevance to solar panel reuse. Key aspects of product reuse such as safety, quality, and technical standards are explored, highlighting the unique challenges associated with the long lifespans and environmental exposure of solar panels. Through this analysis, this study reveals the core elements vital for an effective certification framework. While structured certification frameworks are essential for sustainability, empirical evidence on their effectiveness in the solar panel reuse remains scarce, and regulatory inconsistencies add complexity. Using established practices in electronics, batteries, and other high-liability sectors as an anchor, the proposed framework, emerging from this systematic review, aims to extend solar panels’ lifecycle, contributing to environmental sustainability and socio-economic equity. The findings provide valuable insights for policymakers, industry stakeholders, and researchers by addressing key certification gaps and identifying future research directions in solar panel reuse standardization. Full article

Air quality and environmental issues have gained attention from countries and organizations worldwide over the past several decades. In recent years, carbon peak and carbon neutrality have been mentioned at many international conferences and meetings aimed at reducing and controlling environmental challenges. This study focuses on trend analysis and expectations for the duration of control for environmental air quality (EAQ) indicators, assesses the current EAQ conditions across global countries, and presents reasonable suggestions for environmental control. The study begins by examining the annual, per capita, and per square meter (${\mathrm{m}}^2$) carbon dioxide (CO₂) emission peak and standardizations, where carbon standardization is a replacement for carbon neutrality. A similar quantitative methodology was employed to assess classical air quality factors such as sulfur dioxide (SO₂) and nitrogen oxides (NO_x). The findings suggest that the average control year length (ACYL) of NO_x is longer than that of SO₂, and the ACYL of SO₂ is, in turn, longer than that of CO₂. From an energy structure perspective, regressions results indicate that biofuel and wind power contribute to improvements in EAQ, while coal, oil, and gas power exert negative impacts. Moreover, a long-term EAQ model utilizing an adjusted max–min normalization method is proposed to integrate various EAQ indicators. This study also presents an EAQ ranking for global countries and recommends countries with critical EAQ challenges. The results demonstrate that it is plausible to control EAQ factors at an excellent level with advances in control technologies and effective measures by government, industries, and individuals. Full article

In recent years, functional foods have garnered increasing scientific and public health interest due to their potential to confer physiological benefits beyond basic nutritional value. International bodies such as EFSA, FDA, and WHO define functional foods as those containing bioactive components that may contribute to the prevention and management of chronic non-communicable diseases, including cardiovascular disease, type 2 diabetes, and certain cancers. The evolving paradigm of “food as medicine” reflects a broader shift in nutritional science towards proactive, health-oriented dietary strategies. This article provides a comprehensive, interdisciplinary overview of functional foods by examining their biological mechanisms, clinical evidence, public health significance, regulatory frameworks, and future prospects—particularly in the context of advances in personalized nutrition and nutrigenomics. A thorough literature review was conducted, drawing from recent peer-reviewed studies and guidelines from key health authorities. The review highlights the roles of specific compounds such as probiotics and prebiotics in modulating the gut microbiome, flavonoids and polyphenols in anti-inflammatory processes, omega-3 fatty acids in cardiometabolic regulation, and vitamins and minerals in supporting immune function. While an expanding body of clinical trials and meta-analyses supports the health benefits of these compounds—including reductions in LDL cholesterol, improved insulin sensitivity, and mitigation of oxidative stress—the integration of functional foods into everyday diets remains challenging. Socioeconomic disparities and limited health literacy often impede their accessibility and widespread adoption in public health practice. Functional foods represent a promising component of prevention-focused modern healthcare. To maximize their impact, a coordinated, evidence-based approach is essential, involving collaboration among healthcare professionals, nutrition scientists, policymakers, and the food industry. Looking forward, innovations in artificial intelligence, microbiome research, and genomic technologies may unlock novel opportunities for the targeted and effective application of functional foods in population health. Full article

Pyrazolopyridines and pyrazolopyrimidines are 5:6 aza-fused N-heteroaromatic compounds (NHACs) comprising a pyrazole ring fused to a pyridine or pyrimidine ring. They exhibit dipolar behavior due to their π-excessive and π-deficient characteristics conferred by their five- and six-membered rings. These features favor their stability, reactivity, and structural diversity, offering numerous modular and functional derivatives (e.g., pyrazolo[1,2-a]pyridines, pyrazolo[1,5-a]pyrimidines, etc.). They have been utilized to obtain relevant chemicals in pharmaceuticals, photophysics, industry, and materials science; thus, their synthesis is highly desirable for discovering novel or improved applications. Therefore, this review focuses on recent advances in the synthesis and applications of these compounds, considering reports from the last decade (2015–2024), with particular emphasis on photophysics, as they contain dipolar 5:6 aza-fused rings as essential scaffolds for this purpose. Full article