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Search Results (1,055)

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26 pages, 4818 KiB  
Article
Novel Anion-Exchange Resins for the Effective Recovery of Re(VII) from Simulated By-Products of Cu-Mo Ore Processing
by Piotr Cyganowski, Pawel Pohl, Szymon Pawlik and Dorota Jermakowicz-Bartkowiak
Int. J. Mol. Sci. 2025, 26(15), 7563; https://doi.org/10.3390/ijms26157563 - 5 Aug 2025
Abstract
The efficient recovery of rhenium (Re), a critical metal in high-tech industries, is essential to address its growing demand and reduce reliance on primary mining. In this study, we developed novel anion-exchange resins for the selective adsorption and recovery of Re(VII) ions from [...] Read more.
The efficient recovery of rhenium (Re), a critical metal in high-tech industries, is essential to address its growing demand and reduce reliance on primary mining. In this study, we developed novel anion-exchange resins for the selective adsorption and recovery of Re(VII) ions from acidic solutions, simulating industrial by-products. The resins were synthesized from a vinylbenzyl chloride-co-divinylbenzene copolymer modified with aliphatic, heterocyclic, and aromatic weakly basic amines, selected from among bis(3-aminopropyl)amine (BAPA), 1-(2-pyrimidinyl)piperazine (PIP), thiosemicarbazide (TSC), 2-amino-3-hydroxypyridine (AHP), 1-(2-hydroxyethyl)piperazine (HEP), 4-amino-2,6-dihydroxypyrimidine (AHPI), and 2-thiazolamine (TA). The adsorption of Re on BAPA, PIP, and HEP resins obeyed the Langmuir model, and the resins exhibited high adsorption capacities, with maximum values reaching 435.4 mg Re g−1 at pH 6. Furthermore, strong selectivity for ReO4 ions over competing species, including Mo, Cu, and V, was noted in solutions simulating the leachates of the by-products of Cu-Mo ores. Additionally, complete elution of Re was possible. The developed resins turned out to be highly suitable for the continuous-flow-mode adsorption of ReO4, revealing outstanding adsorption capacities before reaching column breakthrough. In this context, the novel anion-exchange resins developed offer a reference for further Re recovery strategies. Full article
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20 pages, 3741 KiB  
Article
Use of Amino Acids and Organic Waste Extracts to Improve the Quality of Liquid Nitrogen–Calcium–Magnesium Fertilizers
by Eglė Didžiulytė and Rasa Šlinkšienė
Sustainability 2025, 17(15), 7081; https://doi.org/10.3390/su17157081 - 5 Aug 2025
Abstract
Agriculture is one of the most important sectors of the global economy, but it increasingly faces sustainability challenges in meeting rising food demands. The intensive use of mineral fertilizers not only improves yields, but also causes negative environmental impacts such as increasing greenhouse [...] Read more.
Agriculture is one of the most important sectors of the global economy, but it increasingly faces sustainability challenges in meeting rising food demands. The intensive use of mineral fertilizers not only improves yields, but also causes negative environmental impacts such as increasing greenhouse gas emissions, water eutrophication, and soil degradation. To develop more sustainable solutions, the focus is on organic fertilizers, which are produced using waste and biostimulants such as amino acids. The aim of this study was to develop and characterize liquid nitrogen–calcium–magnesium fertilizers produced by decomposing dolomite with nitric acid followed by further processing and to enrich them with a powdered amino acid concentrate Naturamin-WSP and liquid extracts from digestate, a by-product of biogas production. Nutrient-rich extracts were obtained using water and potassium hydroxide solutions, with the latter proving more effective by yielding a higher organic carbon content (4495 ± 0.52 mg/L) and humic substances, which can improve soil structure. The produced fertilizers demonstrated favourable physical properties, including appropriate viscosity and density, as well as low crystallization temperatures (eutectic points from –3 to –34 °C), which are essential for storage and application in cold climates. These properties were achieved by adjusting the content of nitrogenous compounds and bioactive extracts. The results of the study show that liquid fertilizers enriched with organic matter can be an effective and more environmentally friendly alternative to mineral fertilizers, contributing to the development of the circular economy and sustainable agriculture. Full article
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14 pages, 31608 KiB  
Article
Primary Metabolic Variations in Maize Plants Affected by Different Levels of Nitrogen Supply
by The Ngoc Phuong Nguyen, Rose Nimoh Serwaa and Jwakyung Sung
Metabolites 2025, 15(8), 519; https://doi.org/10.3390/metabo15080519 - 1 Aug 2025
Viewed by 186
Abstract
Background/Objectives: Nitrogen (N) is an essential macronutrient that strongly influences maize growth and metabolism. While many studies have focused on nitrogen responses during later developmental stages, early-stage physiological and metabolic responses remain less explored. This study investigated the effect of different nitrogen-deficient [...] Read more.
Background/Objectives: Nitrogen (N) is an essential macronutrient that strongly influences maize growth and metabolism. While many studies have focused on nitrogen responses during later developmental stages, early-stage physiological and metabolic responses remain less explored. This study investigated the effect of different nitrogen-deficient levels on maize seedling growth and primary metabolite profiles. Methods: Seedlings were treated with N-modified nutrient solution, which contained 0% to 120% of the standard nitrogen level (8.5 mM). Results: Nitrogen starvation (N0) significantly reduced plant height (by 11–14%), shoot fresh weight (over 30%) compared to the optimal N supply (N100). Total leaf nitrogen content under N0–N20 was less than half of that in N100, whereas moderate N deficiency resulted in moderate reductions in growth and nitrogen content. Metabolite analysis revealed that N deficiency induced the accumulation of soluble sugars and organic acids (up to threefold), while sufficient N promoted the synthesis of amino acids related to nitrogen assimilation and protein biosynthesis. Statistical analyses (PCA and ANOVA) showed that both genotypes (MB and TYC) and tissue type (upper vs. lower leaves) influenced the metabolic response to nitrogen, with MB displaying more consistent shifts and TYC exhibiting greater variability under moderate stress. Conclusions: These findings highlight the sensitivity of maize seedlings to early nitrogen deficiency, with severity influenced by nitrogen level, tissue-specific position, and genotype; thus underscore the close coordination between physiological growth and primary metabolic pathways in response to nitrogen availability. These findings expand current knowledge of nitrogen response mechanisms and offer practical insights for improving nitrogen use efficiency in maize cultivation. Full article
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14 pages, 990 KiB  
Article
Comparative Analysis of the Biomass Production and Nutritional Profiles of Two Wild-Type Strains of Yarrowia lipolytica
by David Torres-Añorve and Georgina Sandoval
Appl. Microbiol. 2025, 5(3), 77; https://doi.org/10.3390/applmicrobiol5030077 - 1 Aug 2025
Viewed by 111
Abstract
Sustainability represents a significant global challenge, requiring a balance between environmental impact and the use of natural resources. White biotechnology, which uses microorganisms and enzymes for environmentally friendly products and processes, offers promising solutions to support a growing population. Within this context, the [...] Read more.
Sustainability represents a significant global challenge, requiring a balance between environmental impact and the use of natural resources. White biotechnology, which uses microorganisms and enzymes for environmentally friendly products and processes, offers promising solutions to support a growing population. Within this context, the yeast Yarrowia lipolytica stands out, so we investigated the generation of biomass from two wild strains (ATCC 9773 and NRRL Y-50997) using different carbon sources. Additionally, protein content and amino acid profiles were assessed via standardized analytical methods to evaluate their potential as nutritional yeasts. Both strains demonstrated potential as nutritional yeasts, with biomass productivities of up to 35.5 g/L and 42 g/L, respectively. The protein content was high, with 58.8% for ATCC 9773 and 58.2% for NRRL Y-50997. Furthermore, the strains presented essential amino acid contents of 62.6% and 41.5%, with lysine being the most abundant amino acid. These findings underscore the versatility and productivity of Y. lipolytica, highlighting its potential for sustainable biotechnological applications such as single-cell protein production. Full article
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30 pages, 703 KiB  
Review
Fungal Lytic Polysaccharide Monooxygenases (LPMOs): Functional Adaptation and Biotechnological Perspectives
by Alex Graça Contato and Carlos Adam Conte-Junior
Eng 2025, 6(8), 177; https://doi.org/10.3390/eng6080177 - 1 Aug 2025
Viewed by 292
Abstract
Fungal lytic polysaccharide monooxygenases (LPMOs) have revolutionized the field of biomass degradation by introducing an oxidative mechanism that complements traditional hydrolytic enzymes. These copper-dependent enzymes catalyze the cleavage of glycosidic bonds in recalcitrant polysaccharides such as cellulose, hemicellulose, and chitin, through the activation [...] Read more.
Fungal lytic polysaccharide monooxygenases (LPMOs) have revolutionized the field of biomass degradation by introducing an oxidative mechanism that complements traditional hydrolytic enzymes. These copper-dependent enzymes catalyze the cleavage of glycosidic bonds in recalcitrant polysaccharides such as cellulose, hemicellulose, and chitin, through the activation of molecular oxygen (O2) or hydrogen peroxide (H2O2). Their catalytic versatility is intricately modulated by structural features, including the histidine brace active site, surface-binding loops, and, in some cases, appended carbohydrate-binding modules (CBMs). The oxidation pattern, whether at the C1, C4, or both positions, is dictated by subtle variations in loop architecture, amino acid microenvironments, and substrate interactions. LPMOs are embedded in a highly synergistic fungal enzymatic system, working alongside cellulases, hemicellulases, lignin-modifying enzymes, and oxidoreductases to enable efficient lignocellulose decomposition. Industrial applications of fungal LPMOs are rapidly expanding, with key roles in second-generation biofuels, biorefineries, textile processing, food and feed industries, and the development of sustainable biomaterials. Recent advances in genome mining, protein engineering, and heterologous expression are accelerating the discovery of novel LPMOs with improved functionalities. Understanding the balance between O2- and H2O2-driven mechanisms remains critical for optimizing their catalytic efficiency while mitigating oxidative inactivation. As the demand for sustainable biotechnological solutions grows, this narrative review highlights how fungal LPMOs function as indispensable biocatalysts for the future of the Circular Bioeconomy and green industrial processes. Full article
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29 pages, 953 KiB  
Review
Comprehensive Review of Alternative Proteins in Pet Food: Research Publications, Patents, and Product Trends in Plant, Aquatic, Insect, and Cell-Based Sources
by Phatthranit Klinmalai, Pitiya Kamonpatana, Arisara Thongpech, Janenutch Sodsai, Khwanchat Promhuad, Atcharawan Srisa, Yeyen Laorenza, Attawit Kovitvadhi, Sathita Areerat, Anusorn Seubsai, Shyam S. Sablani and Nathdanai Harnkarnsujarit
Foods 2025, 14(15), 2640; https://doi.org/10.3390/foods14152640 - 28 Jul 2025
Viewed by 454
Abstract
The increasing demand for sustainable pet-food solutions has driven interest in alternative protein sources, as researchers seek to avoid allergenic foods while maintaining optimal pet nutrition. This review explores recent scientific publications, patent trends, and market trends relating to various alternative protein sources, [...] Read more.
The increasing demand for sustainable pet-food solutions has driven interest in alternative protein sources, as researchers seek to avoid allergenic foods while maintaining optimal pet nutrition. This review explores recent scientific publications, patent trends, and market trends relating to various alternative protein sources, including plant-based, aquatic, insect-derived, and cell-based sources. Their nutritional composition, functional properties, and potential benefits for pet health were assessed. Plant-based proteins, such as soy, pea, and lentils, provide essential amino acids and functional properties suitable for meat analogues. Microalgae and seaweed offer rich sources of omega-3 fatty acids, antioxidants, and bioactive compounds. Insect-based proteins such as black-soldier-fly larvae and mealworms are highly digestible and rich in essential nutrients, with additional benefits for gut health. Emerging cell-based proteins present a novel, lab-grown alternative with promising sustainability and nutritional advantages. While these protein sources offer significant benefits, challenges related to digestibility, palatability, regulatory approval, and consumer acceptance must be addressed. The emphasis of the present research is on current developments for industry uses and future potential. The analysis sheds light on the contributions of alternative protein sources to the promotion of sustainable and nutrient meals for pets. Full article
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30 pages, 3715 KiB  
Article
The Inhibitory Effect and Adsorption Properties of Testagen Peptide on Copper Surfaces in Saline Environments: An Experimental and Computational Study
by Aurelian Dobriţescu, Adriana Samide, Nicoleta Cioateră, Oana Camelia Mic, Cătălina Ionescu, Irina Dăbuleanu, Cristian Tigae, Cezar Ionuţ Spînu and Bogdan Oprea
Molecules 2025, 30(15), 3141; https://doi.org/10.3390/molecules30153141 - 26 Jul 2025
Viewed by 449
Abstract
Experimental and theoretical studies were applied to investigate the adsorption properties of testagen (KEDG) peptide on copper surfaces in sodium chloride solution and, implicitly, its inhibition efficiency (IE) on metal corrosion. The tetrapeptide synthesized from the amino acids lysine (Lys), glutamic acid (Glu), [...] Read more.
Experimental and theoretical studies were applied to investigate the adsorption properties of testagen (KEDG) peptide on copper surfaces in sodium chloride solution and, implicitly, its inhibition efficiency (IE) on metal corrosion. The tetrapeptide synthesized from the amino acids lysine (Lys), glutamic acid (Glu), aspartic acid (Asp), and glycine (Gly), named as H-Lys-Glu-Asp-Gly-OH, achieved an inhibition efficiency of around 86% calculated from electrochemical measurements, making KEDG a promising new copper corrosion inhibitor. The experimental data were best fitted to the Freundlich adsorption isotherm. The standard free energy of adsorption (ΔGadso) reached the value of −30.86 kJ mol−1, which revealed a mixed action mechanism of tetrapeptide, namely, chemical and physical spontaneous adsorption. The copper surface characterization was performed using optical microscopy and SEM/EDS analysis. In the KEDG presence, post-corrosion, SEM images showed a network surface morphology including microdeposits with an acicular appearance, and EDS analysis highlighted an upper surface layer consisting of KEDG, sodium chloride, and copper corrosion compounds. The computational study based on DFT and Monte Carlo simulation confirmed the experimental results and concluded that the spontaneous adsorption equilibrium establishment was the consequence of the contribution of noncovalent (electrostatic, van der Waals) interactions and covalent bonds. Full article
(This article belongs to the Section Computational and Theoretical Chemistry)
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15 pages, 5562 KiB  
Article
Effect of Amino Trimethylene Phosphonic Acid and Tartaric Acid on Compressive Strength and Water Resistance of Magnesium Oxysulfate Cement
by Yutong Zhou, Zheng Zhou, Lvchao Qiu, Kuangda Lu, Dongmei Xu, Shiyuan Zhang, Shixuan Zhang, Shouwei Jian and Hongbo Tan
Materials 2025, 18(15), 3473; https://doi.org/10.3390/ma18153473 - 24 Jul 2025
Viewed by 155
Abstract
Organic acids could act as retarders in magnesium oxysulfide (MOS) systems, not only delaying setting and improving fluidity but also enhancing compressive strength and water resistance. These effects are generally attributed to both the presence of H+ ions and anion chelation. However, [...] Read more.
Organic acids could act as retarders in magnesium oxysulfide (MOS) systems, not only delaying setting and improving fluidity but also enhancing compressive strength and water resistance. These effects are generally attributed to both the presence of H+ ions and anion chelation. However, the enhancement efficiency of different organic acids in MOS systems varies significantly due to differences in their molecular structures. To determine the underlying mechanism, this study comparatively investigated the effects of amino trimethylene phosphonic acid (ATMP) and tartaric acid (TA) on the setting time, fluidity, compressive strength, and water resistance of the MOS system, with the two additives incorporated at mole ratios to MgO ranging from 0.002 to 0.006. The mechanism behind it was revealed by discussion on the hydration heat, hydrates, and pH value. Results showed that both ATMP and TA could effectively improve the fluidity, delay the setting process, and enhance the mechanical properties, including strength and water resistance. At a mole ratio of 0.006, the incorporation of ATMP increased the 28 d compressive strength and the softening coefficient by 214.12% and 37.29%, respectively, compared with the blank group. In contrast, under the same dosage, TA led to an increase of 55.13% in the 28 d strength and 22.03% in the softening coefficient. Furthermore, hydration heat, product analysis, and pH measurements indicated that both ATMP and TA inhibited hydration during the initial hours but promoted hydration at later stages. The potential reason could be divided into two aspects: (1) H+ ions from ATMP and TA suppressing the formation of Mg(OH)2; (2) anion chelation with Mg2+ in the liquid phase, leading to a supersaturated solution with higher saturation, which further hindered Mg(OH)2 formation and facilitated the later development of 5Mg(OH)2·MgSO4·7H2O (517 phase). By contrast, under the same mole dosage of H+ or anions, the enhancement in compressive strength as well as the water resistance is superior when using ATMP. This was owing to its stronger chelating ability of ATMP, which more effectively inhibited Mg(OH)2 formation and then promoted the formation of the 517 phase. These findings confirm that the chelating ability of anions exerts an important impact on the retarding effect as well as the enhancement of strength in MOS systems. Full article
(This article belongs to the Section Construction and Building Materials)
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12 pages, 6808 KiB  
Communication
Research on Preventing High-Density Materials from Settling in Liquid Resin
by Lixin Xuan, Zhiqiang Wang, Xuan Yang, Xiao Wu, Junjiao Yang and Shijun Zheng
Materials 2025, 18(15), 3469; https://doi.org/10.3390/ma18153469 - 24 Jul 2025
Viewed by 191
Abstract
The applications of magnetic particles in anti-counterfeiting and anti-absorbing coatings and other functional materials are becoming increasingly widespread. However, due to their high density, the magnetic particles rapidly settle in organic resin media, significantly affecting the quality of the related products. Thereby, reducing [...] Read more.
The applications of magnetic particles in anti-counterfeiting and anti-absorbing coatings and other functional materials are becoming increasingly widespread. However, due to their high density, the magnetic particles rapidly settle in organic resin media, significantly affecting the quality of the related products. Thereby, reducing the density of the particles is essential. To achieve this goal, high-density magnetic particles were coated onto the surface of hollow silica using anion–cation composite technology. Further, the silane coupling agent N-[3-(trimethoxysilyl)propyl]ethylenediamine was bonded to the surface of magnetic particles to form an amino-covered interfacial layer with a pH value of 9.28, while acrylic acid was polymerized and coated onto the surface of hollow silica to form a carboxyl-covered interfacial layer with a pH value of 4.65. Subsequently, the two materials were compounded to obtain a low-density composite magnetic material. The morphologies and structural compositions of the magnetic composite materials were studied by FTIR, SEM, SEM-EDS, XRD, and other methods. The packing densities of the magnetic composite materials were compared using the particle packing method, thereby solving the problem of magnetic particles settling in the resin solution. Full article
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25 pages, 3993 KiB  
Article
Green Chemistry and Multivariate Optimization in the Extraction of Phenolic Compounds: The Potential of NaDES in Alternative Raw Materials for Expanded Extrudates
by Mateus Alves Araújo, Bianca Rodrigues Morais, João Pedro da Silva Santos, Larissa Karla de Jesus, Kaliston Aurélio Lomba, Gustavo Costa do Nascimento, Marcus Alvarenga Soares, Nathalia de Andrade Neves, Irene Andressa, Maria Teresa Pedrosa Silva Clerici and Marcio Schmiele
Methods Protoc. 2025, 8(4), 82; https://doi.org/10.3390/mps8040082 - 23 Jul 2025
Viewed by 367
Abstract
Phenolic compounds are secondary metabolites widely distributed among plants, with bioactive properties, especially antioxidant activity. The search for sustainable extraction methods has driven the use of natural deep eutectic solvents (NaDESs), formed by combinations of natural compounds, such as organic acids, sugars, alcohols, [...] Read more.
Phenolic compounds are secondary metabolites widely distributed among plants, with bioactive properties, especially antioxidant activity. The search for sustainable extraction methods has driven the use of natural deep eutectic solvents (NaDESs), formed by combinations of natural compounds, such as organic acids, sugars, alcohols, and amino acids. This study optimized NaDES (sorbitol, citric acid, and glycine) efficiency and compared it to that of 70% methanol solution in extracting total soluble phenolic compounds (TSPCs) from six flours matrices—corn, buckwheat, biofortified orange sweet potato, red lentil, Sudan grass, and chickpea—before and after thermoplastic extrusion cooking. Quantification was performed using the Folin–Ciocalteu method, with statistical analysis at the 10% significance level. In general, the methanolic extracts showed higher TSPC levels in the raw materials, whereas the levels were higher in NaDESs for legumes. After extrusion, a reduction in the TSPC levels was observed, except in the sweet potato. Multivariate analysis (PLS-DA and heatmap) distinguished the raw and extruded samples, revealing structural and chemical changes from thermal processing. The AGREE scores were 0.7 (NaDES) and 0.54 (methanol), favoring NaDES. The BAGI score (75.0) confirmed the method’s robustness and suitability for sustainable analytical applications. Full article
(This article belongs to the Collection Green Chemistry)
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19 pages, 4720 KiB  
Review
Changes in Thermodynamic Parameters Induced by Pyrimidine Nucleic Bases Forming Complexes with Amino Acids and Peptides in a Buffer Solution at pH = 7.4
by Elena Yu. Tyunina, Vladimir P. Barannikov and Igor N. Mezhevoi
Liquids 2025, 5(3), 19; https://doi.org/10.3390/liquids5030019 - 22 Jul 2025
Viewed by 198
Abstract
This article presents a mini-review of the available data on the thermodynamics of the complexation of amino acids and peptides with some nucleic bases in a buffer medium. Data on changes in thermodynamic parameters (binding constants, Gibbs energy, enthalpy, entropy) during the complexation [...] Read more.
This article presents a mini-review of the available data on the thermodynamics of the complexation of amino acids and peptides with some nucleic bases in a buffer medium. Data on changes in thermodynamic parameters (binding constants, Gibbs energy, enthalpy, entropy) during the complexation of nucleic bases with amino acids and peptides as a function of physicochemical properties are given at T = 298.15 K. The effects of complexation on the volumetric properties of nucleic bases, including apparent molar volumes, standard molar volumes, and limiting molar expansibility, over a temperature range of 288.15 to 313.15 K are considered in detail. Differences in the behavior of amino acids and peptides caused by different modes of coordination with nucleic bases are noted. These manifest in the stoichiometry of the formed complexes, the relationship with the acid dissociation constants of carboxyl and amino groups, enthalpy–entropy compensation in the complexation process, the temperature dependence of the transfer volumes, and the effect of hydrophobicity on volumetric characteristics. Full article
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14 pages, 3055 KiB  
Article
High-Performance Thin Film Composite Nanofiltration (NF) Membrane Constructed on Modified Polyvinylidene Fluoride (PVDF) Substrate
by Junliang Dong, Qianzhi Sun, Xiaolin Feng and Ruijun Zhang
Membranes 2025, 15(7), 216; https://doi.org/10.3390/membranes15070216 - 20 Jul 2025
Viewed by 385
Abstract
The inherent hydrophobic nature of PVDF material renders it challenging to establish a stable aqueous hydration layer, thereby limiting its suitability as a substrate for the preparation of nanofiltration (NF) membranes. In this study, we developed a novel modification approach that effectively enhances [...] Read more.
The inherent hydrophobic nature of PVDF material renders it challenging to establish a stable aqueous hydration layer, thereby limiting its suitability as a substrate for the preparation of nanofiltration (NF) membranes. In this study, we developed a novel modification approach that effectively enhances the hydrophilicity of PVDF substrates through the incorporation of sulfonic acid-doped polyaniline (SPANI) and hyperbranched polyester (HPE) into the PVDF casting solution, followed by cross-linking with trimesoyl chloride (TMC). The introduction of SPANI and HPE, which contain reactive polar amino and hydroxyl groups, improved the hydrophilicity of the substrate, while the subsequent cross-linking with TMC effectively anchored these components within the substrate through the covalent linking between TMC and the reactive sites. Additionally, the hydrolysis of TMC yielded non-reactive carboxyl groups, which further enhanced the hydrophilicity of the substrate. As a result, the modified PVDF substrate exhibited improved hydrophilicity, facilitating the construction of an intact polyamide layer. In addition, the fabricated TFC NF membrane demonstrated excellent performance in the advanced treatment of tap water, achieving a total dissolved solid removal rate of 57.9% and a total organic carbon removal rate of 85.3%. This work provides a facile and effective route to modify PVDF substrates for NF membrane fabrication. Full article
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20 pages, 16304 KiB  
Article
Functional Analysis of the Cyclin E Gene in the Reproductive Development of Rainbow Trout (Oncorhynchus mykiss)
by Enhui Liu, Haixia Song, Wei Gu, Gaochao Wang, Peng Fan, Kaibo Ge, Yunchao Sun, Datian Li, Gefeng Xu and Tianqing Huang
Biology 2025, 14(7), 862; https://doi.org/10.3390/biology14070862 - 16 Jul 2025
Viewed by 308
Abstract
As a commercially valuable aquaculture species, rainbow trout (Oncorhynchus mykiss) urgently require solutions to growth inhibition associated with reproductive development. To elucidate the function of the cell cycle regulator Cyclin E genes (CCNE1 and CCNE2) in this process, we [...] Read more.
As a commercially valuable aquaculture species, rainbow trout (Oncorhynchus mykiss) urgently require solutions to growth inhibition associated with reproductive development. To elucidate the function of the cell cycle regulator Cyclin E genes (CCNE1 and CCNE2) in this process, we cloned the genes and analyzed their relative expression across various tissues and gonadal developmental stages. Using RNA interference (RNAi) and overexpression in RTG2 cells, we examined the effects of CCNE on cell viability, proliferation, and meiotic gene expression. Results showed that the open reading frame lengths of CCNE1 and CCNE2 were 1230 bp and 1188 bp, encoding 408 and 395 amino acids, respectively. Both proteins contain two conserved cyclin boxes, exhibit high structural similarity, and are phylogenetically most closely related to Oncorhynchus tshawytscha and Oncorhynchus kisutch. Expression and localization analyses revealed that CCNE1 was highly expressed in the ovary, while CCNE2 was highly expressed in the testis. Both proteins were expressed during fertilized egg development and key gonadal stages (at 13, 21, and 35 months post-fertilization). CCNE expression positively correlated with RTG2 cell viability and proliferation, with immunofluorescence confirming that CCNE is localized in the nucleus. Knockdown or overexpression of CCNE induced the differential expression of reproductive-related genes and key meiotic regulators. These findings suggest that CCNE1 and CCNE2 balance meiosis and gamete development through specific regulatory mechanisms, and their dysregulation may be a key factor underlying meiosis inhibition and reproductive development abnormalities. Full article
(This article belongs to the Special Issue Aquatic Economic Animal Breeding and Healthy Farming)
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13 pages, 293 KiB  
Article
Amino Acids as Biostimulants: Effects on Growth, Chlorophyll Content, and Antioxidant Activity in Ocimum basilicum L.
by Justina Deveikytė, Aušra Blinstrubienė and Natalija Burbulis
Agriculture 2025, 15(14), 1496; https://doi.org/10.3390/agriculture15141496 - 11 Jul 2025
Viewed by 329
Abstract
It is necessary to explore possibilities to increase agricultural production in environmentally friendly ways while maintaining the quality standards of plant raw materials. The effect of amino acids on sweet basil (Ocimum basilicum L.) development may stimulate biomass accumulation and enhance the [...] Read more.
It is necessary to explore possibilities to increase agricultural production in environmentally friendly ways while maintaining the quality standards of plant raw materials. The effect of amino acids on sweet basil (Ocimum basilicum L.) development may stimulate biomass accumulation and enhance the biosynthesis of secondary metabolites. Investigated varieties “Rosie”, “Red Opal”, “Bordeaux”, “Dark Opal”, “Red Rubin”, “Genovese”, “Cinamon”, “Italiano Classico”, “Marseillais”, and “Thai” were cultivated in a controlled-environment growth chamber and the impact of isoleucine, methionine, glutamine, tryptophan, phenylalanine was studied on biomass accumulation, chlorophyll and phenolic content, and antioxidant activity. Five to six true leaves plants were treated once with an aqueous solution containing 100 mg L−1 of the mentioned amino acids or received no treatment. Our results show that methionine or tryptophan improved the most fresh and dry weight of shoot system of sweet basil plants. Methionine increased chlorophyl a content in 6 of 10 sweet basil varieties, while glutamine had the greatest results in chlorophyl b content. Phenylalanine increased total phenolic content in most treated plants, as well as antioxidant activity. Amino acids may be applied as useful biostimulants in modern agriculture, as they play an important role in ensuring sustainable crop productivity, fostering beneficial plant properties. Full article
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23 pages, 990 KiB  
Review
Toward Sustainable Broiler Production: Evaluating Microbial Protein as Supplementation for Conventional Feed Proteins
by Daniela-Mihaela Grigore, Maria-Luiza Mircea and Elena Narcisa Pogurschi
Agriculture 2025, 15(14), 1486; https://doi.org/10.3390/agriculture15141486 - 10 Jul 2025
Viewed by 408
Abstract
The increasing demand for sustainable poultry production has urged the exploration of alternative feed strategies supporting animal performance and environmental goals. The first section outlines the protein requirements in broiler nutrition (19–25% crude protein) and the physiological importance of balanced amino acid profiles. [...] Read more.
The increasing demand for sustainable poultry production has urged the exploration of alternative feed strategies supporting animal performance and environmental goals. The first section outlines the protein requirements in broiler nutrition (19–25% crude protein) and the physiological importance of balanced amino acid profiles. Vegetal conventional protein sources are discussed in terms of their nutritional value (12.7–20.1 MJ/kg), limitations (antinutritional factors), and availability. Emerging trends in broiler nutrition highlight the integration of supplements and the need for innovative feed solutions as support for the improvement in broiler body weight and feed efficiency increase. Microbial protein sources: yeast biomass (41–60% of 100 g dry weight), microbial mixed cultures (32–76% of 100 g dry weight), and beer by-products, such as brewer’s spent yeast (43–52% of 100 g dry weight), offer promising nutritional profiles, rich in bioactive compounds (vitamin B complex, minerals, enzymes, and antioxidants), and may contribute to improved gut health, immunity, and feed efficiency when used as dietary supplements. The review also addresses the regulatory and safety considerations associated with the use of microbial protein in animal feed, emphasizing EU legislation and standards. Finally, recent findings on the impact of microbial protein supplementation on broiler growth performance, carcass traits, and overall health status are discussed. This review supports the inclusion of microbial protein sources as valuable co-nutrients that complement conventional feed proteins, contributing to more resilient and sustainable broiler production and broiler meat products. Full article
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