Search Results (796)

Accurate above-ground biomass (AGB) quantification is confounded by signal saturation and data fusion challenges, particularly in structurally complex ecosystems like bamboo forests. To address these gaps, this study developed a two-stage framework to map the AGB of Dendrocalamus giganteus in a subtropical mountain environment. This study first employed Empirical Bayesian Kriging Regression Prediction (EBKRP) to spatialize sparse GEDI and ICESat-2 LiDAR metrics using Sentinel-2 and topographic covariates. Subsequently, a stacked ensemble model, integrating four machine learning algorithms, predicted AGB from the full suite of continuous variables. The stacking model achieved high predictive accuracy (R² = 0.84, RMSE = 11.07 Mg ha⁻¹) and substantially mitigated the common bias of underestimating high AGB, improving the predicted observed regression slope from a base model average of 0.63 to 0.81. Furthermore, SHAP analysis provided mechanistic insights, identifying the canopy photon rate as the dominant predictor and quantifying the ecological thresholds governing AGB distribution. The mean AGB density was 71.8 ± 21.9 Mg ha⁻¹, with its spatial pattern influenced by elevation and human settlements. This research provides a robust framework for synergizing multi-source remote sensing data to improve AGB estimation, offering a refined methodological pathway for large-scale carbon stock assessments. Full article

Leaf chlorophyll content (LCC), leaf area index (LAI), and above-ground biomass (AGB) are important growth parameters for characterizing potato growth and predicting yield. While deep learning has demonstrated remarkable advancements in estimating crop growth parameters, the limited availability of field data often compromises model accuracy and generalizability, impeding large-scale regional applications. This study proposes a novel deep learning model that integrates multi-task learning and few-shot learning to address the challenge of low data in growth parameter prediction. Two multi-task learning architectures, MTL-DCNN and MTL-MMOE, were designed based on deep convolutional neural networks (DCNNs) and multi-gate mixture-of-experts (MMOE) for the simultaneous estimation of LCC, LAI, and AGB from Sentinel-2 imagery. Building on this, a few-shot learning framework for growth prediction (FSLGP) was developed by integrating simulated spectral generation, model-agnostic meta-learning (MAML), and meta-transfer learning strategies, enabling accurate prediction of multiple growth parameters under limited data availability. The results demonstrated that the incorporation of calibrated simulated spectral data significantly improved the estimation accuracy of LCC, LAI, and AGB (R² = 0.62~0.73). Under scenarios with limited field measurement data, the multi-task deep learning model based on few-shot learning outperformed traditional mixed inversion methods in predicting potato growth parameters (R² = 0.69~0.73; rRMSE = 16.68%~28.13%). Among the two architectures, the MTL-MMOE model exhibited superior stability and robustness in multi-task learning. Independent spatiotemporal validation further confirmed the potential of MTL-MMOE in estimating LAI and AGB across different years and locations (R² = 0.37~0.52). These results collectively demonstrated that the proposed FSLGP framework could achieve reliable estimation of crop growth parameters using only a very limited number of in-field samples (approximately 80 samples). This study can provide a valuable technical reference for monitoring and predicting growth parameters in other crops. Full article

The background for this study is the limitations of the conventional approach of using deforestation area multiplied by biomass densities or emission factors. We demonstrated how TanDEM-X and GEDI data can be combined to estimate forest Above Ground Biomass (AGB) change at the national scale for Tanzania. The results can be further recalculated to estimate CO₂ emissions and removals from the forest. We used repeated short wavelength, InSAR DEMs from TanDEM-X to derive changes in forest canopy height and combined this with GEDI data to convert such height changes to AGB changes. We estimated AGB change during 2012–2019 to be −2.96 ± 2.44 MT per year. This result cannot be validated, because the true value is unknown. However, we corroborated the results by comparing with other approaches, other datasets, and the results of other studies. In conclusion, TanDEM-X and GEDI can be combined to derive reliable temporal change in AGB at large scales such as a country. An important advantage of the method is that it is not required to have a representative field inventory plot network nor a full coverage DTM. A limitation for applying this method now is the lack of frequent and systematic InSAR elevation data. Full article

Quinoa (Chenopodium quinoa Willd.) has emerged as a climate-resilient, nutrient-dense crop with increasing global popularity because of its adaptability under current environmental variations. To address the limited understanding of quinoa’s genotypic performance under local agro-environmental conditions, this study hypothesized that elite genotypes would exhibit significant variation in agronomic, physiological, and biochemical traits. This study aimed to elucidate genotypic variability among 23 elite quinoa lines under field conditions in Faisalabad, Pakistan, using a multidimensional framework that integrated phenological, physiological, biochemical, root developmental, and yield-related attributes. The results revealed that significant variation was observed across all measured parameters, highlighting the diverse adaptive strategies and functional capacities among the tested genotypes. More specifically, genotypes Q4, Q11, Q15, and Q126 demonstrated superior agronomic potential and canopy-level physiological efficiencies, including high biomass accumulation, low infrared canopy temperatures and sustained NDVI values. Moreover, Q9 and Q52 showed enhanced accumulation of antioxidant compounds such as phenolics and anthocyanins, suggesting potential for functional food applications and breeding program for improving these traits in high-yielding varieties. Furthermore, root trait analysis revealed Q15, Q24, and Q82 with well-developed root systems, suggesting efficient resource acquisition and sufficient support for above-ground plant parts. Moreover, principal component analysis further clarified genotype clustering based on trait synergistic effects. These findings support the use of multidimensional phenotyping to identify ideotypes with high yield potential, physiological efficiency and nutritional value. The study provides a foundational basis for quinoa improvement programs targeting climate adaptability and quality enhancement. Full article

Remote sensing tools have been proposed to assist with rice crop monitoring but have been developed and validated on ponded rice. This two-year study was conducted on a commercial rice farm with irrigation automation technology aimed to (i) understand how canopy reflectance differs between high-yielding ponded and aerobic rice, (ii) validate the feasibility of using the squared simplified canopy chlorophyll content index (SCCCI²) for N uptake estimates, and (iii) explore the SCCCI² and similar chlorophyll-sensitive indices for grain quality monitoring. Multispectral images were collected from an unmanned aerial vehicle during both rice-growing seasons. Above-ground biomass and nitrogen (N) uptake were measured at panicle initiation (PI). The performance of single-vegetation-index models in estimating rice N uptake, as previously published, was assessed. Yield and grain quality were determined at harvest. Results showed that canopy reflectance in the visible and near-infrared regions differed between aerobic and ponded rice early in the growing season. Chlorophyll-sensitive indices showed lower values in aerobic rice than in the ponded rice at PI, despite having similar yields at harvest. The SCCCI² model (RMSE = 20.52, Bias = −6.21 Kg N ha⁻¹, and MAPE = 11.95%) outperformed other models assessed. The SCCCI², squared normalized difference red edge index, and chlorophyll green index correlated at PI with the percentage of cracked grain, immature grain, and quality score, suggesting that grain milling quality parameters could be associated with N uptake at PI. This study highlights canopy reflectance differences between high-yielding aerobic (averaging 15 Mg ha⁻¹) and ponded rice at key phenological stages and confirms the validity of a single-vegetation-index model based on the SCCCI² for N uptake estimates in ponded and non-ponded rice crops. Full article

Root pruning can promote the transplanting of young green plants, but the overall impact of pruning on root growth, morphology, and physiological functions remains unclear. This study integrated transcriptomics and physiological analyses to elucidate the effects of root pruning on blueberry growth. Appropriate pruning (CT4) significantly promoted plant growth, with above-ground biomass and leaf biomass significantly increasing compared to the control group within 42 days. Photosynthesis temporarily decreased at 7 days but recovered at 21 and 42 days. Transcriptomics analysis showed that the cellulose metabolism pathway was rapidly activated and influenced multiple key genes in the starch metabolism pathway. Importantly, transcription factors associated with vascular development were also significantly increased at 7, 21, and 42 days after root pruning, indicating their role in regulating vascular differentiation. Enhanced aboveground growth was positively correlated with the expression of photosynthesis-related genes, and the transport of photosynthetic products via vascular tissues provided a carbon source for root development. Thus, root development is closely related to leaf photosynthesis, and changes in gene expression associated with vascular tissue development directly influence root development, ultimately ensuring coordinated growth between aboveground and belowground parts. These findings provide a theoretical basis for optimizing root pruning strategies to enhance blueberry growth and yield. Full article

Phosphorus deficiency poses a significant challenge to the growth and productivity of crops, particularly in nutrient-poor soils. This study investigates the effects of phosphorus deficiency on the growth, endogenous phytohormones, metabolome, and transcriptome of sweet potato (Ipomoea batatas L.) over a growth period from 30 to 120 days. We found that low phosphorus conditions significantly reduced both above- and below-ground biomass, while tuber number remained unchanged. Endogenous phytohormone analysis revealed altered levels of abscisic acid (ABA), indole-3-acetic acid (IAA), and cytokinins, indicating a complex hormonal response to phosphorus starvation. Transcriptomic analysis identified a total of 6324 differentially expressed genes (DEGs) at 60 days, with significant enrichment in pathways related to stress response and phosphorus utilization (PAPs and PHO1). Metabolomic profiling revealed notable shifts in key metabolites, with consistent downregulation of several phosphorous-related compounds. Our findings highlight the intricate interplay between growth, hormonal regulation, metabolic reprogramming, and gene expression in response to phosphorus deficiency in sweet potato. This research underscores the importance of understanding nutrient stress responses to enhance sweet potato resilience and inform sustainable agricultural practices. Future research should focus on exploring the potential for genetic and agronomic interventions to mitigate the effects of phosphorus deficiency and optimize sweet potato productivity in challenging environments. Full article

Light Detection and Ranging (LiDAR) provides three-dimensional information that can be used to extract tree parameter measurements such as height (H), canopy volume (CV), canopy diameter (CD), canopy area (CA), and tree stand density. LiDAR data does not directly give diameter at breast height (DBH), an important input into allometric equations to estimate biomass. The main objective of this study is to estimate tree DBH using existing allometric models. Specifically, it compares three global DBH pantropical models to calculate DBH and to estimate the aboveground biomass (AGB) of the Lake Broadwater Forest located in Southeast (SE) Queensland, Australia. LiDAR data collected in mid-2022 was used to test these models, with field validation data collected at the beginning of 2024. The three DBH estimation models—the Jucker model, Gonzalez-Benecke model 1, and Gonzalez-Benecke model 2—all used tree H, and the Jucker and Gonzalez-Benecke model 2 additionally used CD and CA, respectively. Model performance was assessed using five statistical metrics: root mean squared error (RMSE), mean absolute error (MAE), mean absolute percentage error (MAPE), percentage bias (MBias), and the coefficient of determination (R²). The Jucker model was the best-performing model, followed by Gonzalez-Benecke model 2 and Gonzalez-Benecke model 1. The Jucker model had an RMSE of 8.7 cm, an MAE of −13.54 cm, an MAPE of 7%, an MBias of 13.73 cm, and an R² of 0.9005. The Chave AGB model was used to estimate the AGB at the tree, plot, and per hectare levels using the Jucker model-calculated DBH and the field-measured DBH. AGB was used to estimate total biomass, dry weight, carbon (C), and carbon dioxide (CO₂) sequestered per hectare. The Lake Broadwater Forest was estimated to have an AGB of 161.5 Mg/ha in 2022, a Total C of 65.6 Mg/ha, and a CO₂ sequestered of 240.7 Mg/ha in 2022. These findings highlight the substantial carbon storage potential of the Lake Broadwater Forest, reinforcing the opportunity for landholders to participate in the carbon credit systems, which offer financial benefits and enable contributions to carbon mitigation programs, thereby helping to meet national and global carbon reduction targets. Full article

Mangrove forests are vital blue carbon (BC) ecosystems that significantly contribute to climate change mitigation through carbon sequestration. Accurate, scalable, and cost-effective methods for estimating carbon stocks in these environments are essential for conservation planning. In this study, we assessed the potential of drones, also known as unmanned aerial vehicles (UAVs), for estimating above-ground biomass (AGB) and BC in Avicennia marina stands by integrating drone-based canopy measurements with field-measured tree heights. Using structure-from-motion (SfM) photogrammetry and a consumer-grade drone, we generated a canopy height model and extracted structural parameters from individual trees in the Melgonze mangrove patch, southern Iran. Field-measured tree heights served to validate drone-derived estimates and calibrate an allometric model tailored for A. marina. While drone-based heights differed significantly from field measurements (p < 0.001), the resulting AGB and BC estimates showed no significant difference (p > 0.05), demonstrating that crown area (CA) and model formulation effectively compensate for height inaccuracies. This study confirms that drones can provide reliable estimates of BC through non-invasive means—eliminating the need to harvest, cut, or physically disturb individual trees—supporting their application in mangrove monitoring and ecosystem service assessments, even under challenging field conditions. Full article

Accurate quantification of above-ground biomass (AGB) and carbon sequestration is vital for monitoring terrestrial ecosystem dynamics, informing climate policy, and supporting carbon neutrality initiatives. However, conventional methods—ranging from manual field surveys to remote sensing techniques based solely on 2D vegetation indices—often fail to capture the intricate spectral and structural heterogeneity of forest canopies, particularly at fine spatial resolutions. To address these limitations, we introduce ForestIQNet, a novel end-to-end multimodal deep learning framework designed to estimate AGB and associated carbon stocks from UAV-acquired imagery with high spatial fidelity. ForestIQNet combines dual-stream encoders for processing multispectral UAV imagery and a voxelized Canopy Height Model (CHM), fused via a Cross-Attentional Feature Fusion (CAFF) module, enabling fine-grained interaction between spectral reflectance and 3D structure. A lightweight Transformer-based regression head then performs multitask prediction of AGB and CO₂e, capturing long-range spatial dependencies and enhancing generalization. Proposed method achieves an R² of 0.93 and RMSE of 6.1 kg for AGB prediction, compared to 0.78 R² and 11.7 kg RMSE for XGBoost and 0.73 R² and 13.2 kg RMSE for Random Forest. Despite its architectural complexity, ForestIQNet maintains a low inference cost (27 ms per patch) and generalizes well across species, terrain, and canopy structures. These results establish a new benchmark for UAV-enabled biomass estimation and provide scalable, interpretable tools for climate monitoring and forest management. Full article

Accurate biomass estimation is important for forest management and climate change mitigation. This study evaluates the potential of using LiDAR (Light Detection and Ranging) data, acquired through Unmanned Aerial Vehicles (UAVs), for estimating above-ground and total biomass in Eucalyptus globulus and Pinus pinaster stands in central and northern Portugal. The acquired LiDAR point clouds were processed to extract structural metrics such as canopy height, crown area, canopy density, and volume. A multistep variable selection procedure was applied to reduce collinearity and select the most informative predictors. Multiple linear regression (MLR) models were developed and validated using field inventory data. Random Forest (RF) models were also tested for E. globulus, enabling a comparative evaluation between parametric and machine learning regression models. The results show that the 25th height percentile, canopy cover density at two meters, and height variance demonstrated an accurate biomass estimation for E. globulus, with coefficients of determination (R²) varying between 0.86 for MLR and 0.90 for RF. Although RF demonstrated a similar predictive performance, MLR presented advantages in terms of interpretability and computational efficiency. For P. pinaster, only MLR was applied due to the limited number of field data, yet R² exceeded 0.80. Although absolute errors were higher for Pinus pinaster due to greater biomass variability, relative performance remained consistent across species. The results demonstrate the feasibility and efficiency of UAV LiDAR point cloud data for stand-level biomass estimation, providing simple and effective models for biomass estimation in these two species. Full article

Accurate assessment of above-ground biomass (AGB) is essential for optimizing crop growth and enhancing agricultural efficiency. However, predicting above-ground biomass (AGB) presents significant challenges. Traditional point cloud networks often struggle with processing crop structures and data characteristics, hindering their ability to predict biomass accurately. To address these limitations, we propose a point-supervised contrastive deep regression method (PSCDR) and a novel network, BMP_Net (BioMixerPoint_Net). The PSCDR method leverages the benefits of deep contrastive regression while accounting for the modal differences between point cloud and 2D image data. By incorporating Chamfer distance to measure point cloud similarity, it improves the model’s adaptability to point cloud features. Experimental results on the SGCBP public dataset show that PSCDR significantly reduces prediction errors compared to seven other point cloud models. Furthermore, the BMP_Net network, which integrates the novel PFMixer module and a point cloud downsampling module, effectively captures the relationship between point cloud structure, density, and biomass. The model achieved test results with RMSE, MAE, and MAPE values of 75.92, 63.19, and 0.115, respectively, outperforming PointMixer by 37.94, 30.07, and 0.079. This method provides an efficient biomass monitoring tool for precision agriculture. Full article

This study investigates the influence of root cohesion spatial heterogeneity on rainfall-induced landslide distribution across the Loess Plateau, addressing limitations in existing methods that oversimplify root reinforcement. Leveraging Landsat and GaoFen satellite images, we developed a regional root cohesion inversion model that quantifies spatial heterogeneity using tree height (derived from time series Landsat imagery) and above-ground biomass (from 30 m resolution satellite products). This approach, integrated with land use-specific hydrological parameters and an infinite slope stability model, significantly improves landslide susceptibility predictions compared to models ignoring root cohesion or using uniform assignments. High-resolution pre- and post-rainfall GaoFen satellite imagery validated landslide inventories, revealing dynamic susceptibility patterns: farmland exhibited the highest risk, followed by artificial and secondary forests, with susceptibility escalating post-rainfall. This study underscores the critical role of remote sensing-driven root cohesion mapping in landslide risk assessment, offering actionable insights for land use planning and disaster mitigation on the Loess Plateau. Full article

Piriformospora indica is a beneficial endophytic fungus that promotes plant growth and root development by colonizing plant roots. In order to investigate whether P. indica could promote the growth of tissue-cultured Cerasus humilis seedlings, in this study, we co-cultivated P. indica colony segments (P+) and P. indica spore suspensions (P++) in the rooting medium, and plant biomass as well as chlorophyll and root hormone contents of ‘3-19-3’ tissue-cultured C. humilis seedlings were determined under P+, P++, and CK (without fungus inoculation) treatments. The results showed that above-ground biomass and chlorophyll content of P+-and P++-treated tissue-cultured seedlings were significantly increased, and root peroxidase (POD), indole-3-acetic-acid (IAA) content, and root activities were significantly enhanced, while jasmonic acid (JA) and 1-aminocyclopropane-1-carboxylic acid (ACC) contents were reduced. Moreover, the growth-promoting effects of P++ treatment were found to be stronger than those of P+ treatment. Our results confirmed that P. indica was able to promote the growth of tissue-cultured C. humilis seedlings and effectively promoted root development by regulating hormone content. Therefore, the application of P. indica in the production of C. humilis is promising, especially in the cultivation of elite varieties. Full article

Widespread drought conditions have increasingly affected agricultural productivity, requiring the exploration of alternative approaches for improving crop tolerance, yield and quality, since plants adopt many physiological strategies to cope with challenging environments. This study evaluated the effects of a vegetal-derived protein hydrolysate (PH), applied via foliar spray or root drench at a concentration of 3 mL L⁻¹, on tomato plants (n = 96) under well-watered and drought-stressed conditions over a 136-day greenhouse experiment. Overall, sub-optimal irrigation significantly decreased plant dry biomass (−55.3%) and fruit production (−68.8% marketable yield), and enhanced fruit quality in terms of sugar concentration and antioxidant levels. PH treatments, regardless of the application method, did not notably influence above-ground dry biomass, yield, or fruit quality, suggesting that the intensity of drought might have limited PH effectiveness. Metabolomic analysis showed higher concentrations of stress- and quality-related metabolites in tomato fruits from plants under stress, with PH not exerting significant metabolic changes in the fruits. These findings revealed the diminished effectiveness of PHs under severe drought conditions, suggesting that drought stress level needs to be taken into consideration for optimizing biostimulant efficacy. Full article