Search Results (82)

With the increasing importance of securing quantum communication networks, practical and robust entity authentication is a critical requirement. Accordingly, we propose and experimentally validate a quantum entity authentication (QEA) protocol specifically designed for integration with BB84-type quantum key distribution (QKD) workflows and existing terminal architectures. We analyze the protocol’s security against intercept–resend man-in-the-middle (MitM) impersonation, showing that an unauthenticated adversary induces a characteristic 25% correlation error and that the rejection probability approaches unity as the number of detected authentication events increases. For practical realization, the protocol is deployed using weak coherent pulses (WCPs) with decoy-state estimation to bound single-photon contributions and mitigate photon-number-splitting (PNS)-enabled leakage. The system is demonstrated over a field-deployed fiber link of approximately 20 km with ~8 dB optical loss using signal/decoy intensities of ~0.5/~0.15 and sending probabilities 0.88/0.10/0.02 (signal/decoy/vacuum). Across both verification directions, stable operation is observed with quantum bit error rate (QBER) typically fluctuating between 1% and 4% while the sifted key rate remains constant over time. These results provide an experimental basis for integrating physical-layer entity authentication into deployed quantum communication networks. Full article

We compared fish assemblage structure and total mercury (THg) bioaccumulation between a natural floodplain lake and a constructed irrigation canal in central Brazil. A total of 473 individuals representing 34 species were recorded, and dorsal muscle samples from 62 specimens representing shared species or species occupying comparable trophic positions were analyzed for THg (Curimatella immaculata, Hemiodus microlepis, Astyanax aff. bimaculatus, Triportheus albus, Geophagus sveni, Pimelodus blochii, Pygocentrus nattereri, Lycengraulis batesii, and Cichla kelberi). The floodplain lake exhibited higher species richness, diversity, and evenness, whereas the irrigation canal supported a simplified assemblage dominated by fewer species. Total Hg concentrations were significantly higher in the lake than in the irrigation canal; however, this pattern was observed only for the carnivorous guild (t = 5.384, p < 0.0001) and the detritivorous guild (t = 4.183, p = 0.0001). THg increased significantly with trophic level in both systems, from detritivores to carnivores (F₂,₄ = 15.127, p = 0.009), yielding comparable trophic magnification slopes (lake: 1.46, 95% CI: 1.11–1.81; canal: 1.36, 95% CI: 0.94–1.77). Despite lower diversity and THg concentrations in the irrigation canal, Hg transfer efficiency across trophic levels was conserved between systems. Full article

Background: Melanoma is one of the most dangerous types of skin cancer, with its global incidence having surged in recent years. There exists an urgent clinical need for novel therapeutic strategies that combine high efficacy, low toxicity, and multiple mechanisms of action. Methods: This study applies a “Property Optimization and Therapeutic Synergy” strategy, selecting the natural active polysaccharide component, Cordyceps polysaccharides (WCP), as a functional carrier to encapsulate the broad-spectrum chemotherapeutic agent, 10-Hydroxycamptothecin (10HCPT, HCPT). Leveraging non-covalent interactions between the two components, a self-assembly nanoscale drug delivery system (H-W NPs) with high stability and dual antitumor activity was constructed to achieve more efficient and precise antitumor effects. Results: The H-W NPs demonstrated outstanding antitumor efficacy both in vitro and in vivo. The H-W NPs achieved a threefold increase in the inhibition rate against B16-F10 cells compared to free HCPT in vitro and demonstrated a remarkable tumor inhibition rate of 95.08% in vivo. The therapeutic effect may be attributed to the dual antitumor mechanisms of the H-W NPs. Mechanistic studies revealed a synergistic dual-mode of action driving this potent efficacy. Firstly, H-W NPs efficiently induced caspase-3-mediated apoptosis in tumor cells. RNA sequencing analysis suggested the involvement of pathways related to cell cycle arrest and apoptosis. Additionally, H-W NPs promoted the expansion and activation of CD8⁺ T cells in the spleen. These activated cytotoxic T cells reinforced the apoptotic cascade, effectively amplifying the caspase-3-mediated death signal. Conclusions: In summary, the self-assembly nanoscale drug system achieved potent antitumor efficacy through the synergistic action of direct tumor cell killing and immune modulation, offering a highly promising strategy for the development of novel formulations against melanoma. Full article

Recently, interest has grown in using alternatives to cement and aggregates to improve concrete and reduce its environmental impact. This study explores the use of cherry pit waste (CPW) as a partial substitute for coarse aggregates in self-compacting concrete (SCC) at varying rates (0–25%). Rheological, compressive strength, and ultrasonic pulse velocity tests were conducted. The results showed that CPWs reduce flowability but increase cohesion. The 5% CPW mix achieved the highest compressive strength. All the mixes remained acceptable, with classifications from SF3 to SF1. Due to CPWs’ lower density, both wet and dry weights decreased, making this a viable lightweight concrete option. Full article

Waste concrete powder (WCP), characterized by low reactivity and limited utilization potential, is rapidly accumulating due to the increasing volume of demolition and recycling activities, creating significant environmental and resource challenges. Meanwhile, the shortage of natural fine aggregate (NFA) has become increasingly severe. To address these issues, this study develops a sustainable approach that utilizes WCP as the main raw material, together with fly ash (FA), ground granulated blast-furnace slag (GGBFS), ordinary Portland cement (OPC), and sulphoaluminate cement (SAC), to produce a WCP-based artificial fine aggregate (WAFA) through a cold-bonding process. The physical, mechanical, and microstructural properties of WAFA were systematically analyzed, and its concrete performance was evaluated by replacing NFA at 100% volume. The results show that WAFA exhibits a regular spherical morphology and, after grading adjustment, meets the Zone II sand requirements of GB/T 14684-2022. Increasing the cement content from 2% to 10% raises the 28-day single-particle compressive strength (SPCS) from 12.98 MPa to 23.08 MPa (a 77.8% increase), while enhancing WCP reactivity improves SPCS from 16.17 MPa to 22.80 MPa (a 29.1% increase). Higher cement content and WCP reactivity also promote the formation of C–S–H gel and ettringite (AFt), resulting in higher bulk density, reduced water absorption, and a denser microstructure. In concrete applications, WAFA substantially improves workability, with slump values exceeding those of NFA and recycled fine aggregate (RFA) concretes. Although WAFA concrete exhibits slightly lower compressive and splitting tensile strengths compared with NFA concrete, optimized mix design allows the achievement of target strength grades from C30 to C50, with the C50-W10-50 mixture showing the most favorable mechanical performance. In summary, WAFA shows potential for contributing to the high-value utilization of construction waste and the reduction in natural sand consumption. Full article

Although Codonopsis pilosula (common name: Dangshen) is a widely used medicinal edible herb, but the structural divergence and functional specificity of polysaccharides from its intraspecific varieties remain understudied. Therefore, this study explores the structural characteristics and immunomodulatory activities of polysaccharides from two varieties of Codonopsis pilosula, namely, Codonopsis pilosula Nannf. var. modesta (Nannf.) L.T.Shen and Codonopsis pilosula (Franch.) Nannf. Crude polysaccharides were extracted via water extraction–ethanol precipitation and purified using DEAE-52 column chromatography to obtain homogeneous fractions WCP (6.444 kDa) and BCP (15.71 kDa). Structural analyses confirmed both to be fructans, but with distinct linkage patterns: WCP contains Fruf-(2→, Glcp-(1→, →1)-Fruf-(2→, →1,6)-Fruf-(2→ linkages, while BCP additionally harbors →6)-Fruf-(2→ linkages. This structural divergence correlates with the observed differences in their physical properties and biological activities. Using zebrafish models, WCP and BCP were found to increase the density of neutrophils and macrophages, while reducing the levels of pro-inflammatory cytokines including IL-1β, IL-6, and TNF-α (p < 0.05 vs. model group). These results support their immunomodulatory potential, providing a scientific basis for developing Codonopsis polysaccharide-based functional food ingredients. Full article

Higher education institutions face a critical methodological challenge in pursuing net-zero commitments: Within the amount ofhe emissions related to Scope 3, including indirect emissions from water consumption, waste disposal, business travel, and mobility, employees commuting represents 50–92% of campus carbon footprints, yet reliable quantification remains elusive due to fragmented data collection and governance silos. The present research investigates how purposeful integration of the Home-to-Work Commuting Plan (HtWCP)—mandatory under Italian Decree 179/2021—into the Climate Neutrality Plan (CNP) could constitute an innovative strategy to enhance emissions accounting rigor while strengthening institutional governance. Stemming from the University of Genoa case study, we show how leveraging mandatory HtWCP survey infrastructure to collect granular mobility behavioral data (transportation mode, commuting distance, and travel frequency) directly addresses the GHG Protocol-specified distance-based methodology for Scope 3 accounting. In turn, the CNP could support the HtWCP in framing mobility actions into a wider long-term perspective, as well as suggesting a compensation mechanism and paradigm for mobility actions that are currently not included. We therefore establish a replicable model that simultaneously advances three institutional dimensions, through the operationalization of the Avoid–Shift–Improve framework within an integrated workflow: (1) methodological rigor—replacing proxy methodologies with actual behavioral data to eliminate the notorious Scope 3 data gap; (2) governance coherence—aligning voluntary and regulatory instruments to reduce fragmentation and enhance cross-functional collaboration; and (3) adaptive management—embedding biennial feedback cycles that enable continuous validation and iterative refinement of emissions reduction strategies. This framework positions universities as institutional innovators capable of modeling integrated governance approaches with potential transferability to municipal, corporate, and public administration contexts. The findings contribute novel evidence to scholarly literature on institutional sustainability, policy integration, and climate governance, whilst establishing methodological standards relevant to international harmonization efforts in carbon accounting. Full article

This study presents a comprehensive strategy for mitigating drying shrinkage of alkali-activated slag mortar (AASM) with the high-dosage incorporation of waste concrete powder (WCP). Response surface methodology (RSM) coupled with microstructural analysis is used to investigate the synergistic effects of WCP particle size (R), activator modulus (AM), activator content (AC), and water to solid ratio (W/S) on shrinkage behavior and matrix development. The optimized mix—WCP-R = 33.6 µm, AM = 1.23, AC = 6.03%. and W/S = 0.49—exhibits a 120-day drying shrinkage of only 1450.1 µε, significantly lower than that of conventional AASM. Microstructural observations reveal that coarser WCP particles act predominantly as fillers, enhancing stability, whereas finer particles promote gel formation but increase shrinkage. A high AM (1.6) refines the pore structure by reducing large pores (>0.05 µm), while a low W/S (0.46) decreases total porosity to 7.67%, collectively restricting moisture transport. The coexistence of C-(A)-S-H gel and hydrotalcite improves matrix integrity. Notably, this optimized HWAASM achieves a substantially reduced carbon footprint of 180 kg CO₂-eq/t, underscoring its significant environmental advantage. The findings advance the understanding of shrinkage mechanisms in high-WCP-AASM and offer an eco-friendly route for valorizing construction waste and developing low-carbon building materials. Full article

Grazing systems’ production efficiency is a dynamic interaction between soil, pasture, livestock, and climate. The magnitude of the changes is related to the mechanical stress applied by the livestock and their feeding behaviour. In Southern Chile, dairy cattle present a high heterogeneity in breeds, size, live weight, and milk production. This study investigated whether cows of contrasting size/live weight can improve degraded pasture and positively modify soil (Andosol-Duric Hapludand) physical features. Three pasture types were used as follows: (i) cultivated fertilised Lolium perenne L. (perennial ryegrass) and Trifolium repens L. (white clover) mixture (BM); (ii) cultivated fertilised L. perenne, T. repens, Bromus valdivianus Phil. (pasture brome), Holcus lanatus L. (Yorkshire fog), and Dactylis glomerata L. (cocksfoot) mixture (MSM); and (iii) naturalised fertilised pasture Agrostis capillaris L. (browntop), B. valdivianus, and T. repens (NFP). Pastures were grazed with two groups of dairy cows of contrasting size and live weight: light cows (LC) [live weight: 464 ± 5.4 kg; height at the withers: 132 ± 0.6 cm (average ± s.e.m.)] and heavy cows (HC) [live weight: 600 ± 8.7 kg; height at the withers: 141 ± 0.9 cm (average ± s.e.m.)]. Hoof area was measured, and the pressure applied by cows on the soil was calculated. Soil differences in penetration resistance (PR) and macro-porosity (wCP > 50 μm) between pastures were explained by tillage and seeding, rather than as a result of livestock presence and movement (animal trampling). The PR variation during the year was associated with the soil water content (SWC). Grazing dairy cows of contrasting live weight caused changes in soil and pasture attributes, and they behaved differently during grazing. Light cows were linked to more intense grazing, a stable soil structure, and pastures with competitive species and greater tiller density. In MSM, pasture consumption increased, and the soil was more resilient to hoof compression. In general, grazing with heavy cows in these three different pasture systems did not negatively impact soil physical properties. These findings indicate that volcanic soils are resilient and that during renovation, the choice of pasture type has a greater initial impact on soil structure than the selection of cow size, but incorporating lighter cows can be a strategy to promote denser pasture swards in these grazing systems. Full article

This paper addresses the critical challenge of deploying a minimum number of wireless charging pads (WCPs) in obstacle-rich, large-scale Wireless Rechargeable Sensor Networks (WRSNs) to sustain drone operations. We assume a single base station, stationary sensors, convex polygonal obstacles that drones must avoid, and that both the base station and WCPs provide unlimited energy. To solve this, we propose the Outside-to-Inside Onion-Peeling (OIOP) strategy, a novel two-stage algorithm that prioritizes the coverage of the most remote sensors first and then refines the deployment by removing redundant pads while strictly adhering to obstacle constraints. Simulation results demonstrate OIOP’s superior efficiency: it reduces the number of required pads by approximately 10.83% ± 1.30% and 12.16% ± 1.59% compared to state-of-the-art methods (SMC and MC) and achieves execution times that are 58.02% ± 2.44% and 72.09% ± 2.88% faster, respectively. The algorithm also exhibits remarkable robustness, showing the smallest performance degradation as obstacle density increases. Full article

Water use and water environmental protection exhibit highly complex interactions, and their coupling coordination is essential for long-term urban sustainability. This study analyzes the system structure of water utilization, and constructs a water resources–social economy–water environment (WR-SE-WE) system dynamics model for Tianjin and five policy scenarios (business as usual (BAU), water conservation prioritization (WCP), social–economic advancement (SEA), water environmental protection (WEP), and integrated balanced development (IBD)) are simulated. A coupling coordination degree (CCD) model is employed to evaluate scenario performance. The key results show that Tianjin’s WR-SE-WE CCD keeps increasing but differentiates for different scenarios: IBD consistently outperforms all scenarios, achieving an optimal coupling coordination degree of 0.926 by 2035, while the other scenarios rank SEA (0.920) > WEP (0.902) > BAU (0.880) > WCP (0.874). The indicators’ quantitative results exhibit single-policy scenario trade-offs: WCP maximizes water efficiency and pollution control, but severely constrains social economy, offering a temporary solution. WEP excels in water resources supply but limits GDP growth, serving as an effective interim measure. SEA drives rapid economic expansion but strains resources and delays pollution control, making it suitable for long-term planning. Combining the obstacle degree model, four recommendations are proposed, including implementing cross-sector water governance, accelerating the green industrial transition, prioritizing reclaimed water, and scaling agricultural efficiency. These results provide a scientific basis for promoting high-quality regional development in the future. Full article

Chromosomal instability plays a significant role in karyotype evolution and speciation in mammalian groups with notable intraspecific chromosomal variation. The Cervidae family, known for its rapid karyotypic evolution due to chromosomal fragility, shows substantial chromosomal diversity, making it a focal point for studies on chromosomal evolution, particularly with respect to conservation and taxonomic classification. The Amazon gray brocket deer (Passalites nemorivagus) exhibits pronounced chromosomal polymorphism, including two distinct sex chromosome systems: the ancestral XX/XY system and a new system due to an X–autosome fusion (neo-X), where males present XY1Y2. This variation is intriguing, especially given that the effects on hybrids have not been previously reported. This study uses bovine whole-chromosome painting (WCP) and BAC probes to document karyotypic variation in P. nemorivagus. A male with the XY system and a heterozygous autosomal Robertsonian fusion was paired with a female with neo-X chromosomes, and the resulting female offspring displayed an X–autosome fusion in heterozygosity. The females in this study, hybrids for the sex system, exhibited estrus, copulated, and both gave birth to offspring. This characterization is the first step in investigating the effects of sex chromosome system variation on hybrid viability and fertility, and provides insights into the reproductive biology of Neotropical deer. Full article

Bubbling Inception Temperature (BIT) is a critical metric that indicates the temperature at which gas bubbles form due to cellulose decomposition in a paper–oil insulation system. It serves as a key indicator of the thermal stability of transformer insulation, offering valuable insights into its performance under elevated temperatures. Building on findings from a companion study that examined the BIT of Kraft paper (KP), thermally upgraded Kraft paper (TUK), and aramid paper in mineral oil, this research expands the analysis to assess the impact of moisture, aging, and alternative dielectric fluids. Using the same customized experimental setup featuring precise dynamic load control, real-time bubble detection, and continuous monitoring of moisture and temperature, this study evaluates BIT across four distinct oil–paper aging stages: new (0 h) and 2 weeks, 4 weeks, and 6 weeks of accelerated thermal aging. This approach enables a comparative analysis of BIT in various paper–oil systems, focusing on both mineral oil and synthetic esters, as well as the influence of different moisture levels in the paper insulation. The results show that BIT decreases with aging, indicating reduced thermal stability. Furthermore, KP impregnated with synthetic ester exhibits a higher BIT than when impregnated with mineral oil, suggesting that synthetic esters may offer better resistance to bubble formation under thermal stress. Based on these results, empirical BIT models were developed as a function of degree of polymerization (DP) and water content in paper (WCP). This study further demonstrates how these models can be applied to quantify safety margins under emergency overloading conditions, providing a practical tool for operational decision-making in transformer thermal risk management. Full article

As the development of oilfields in China enters its middle-to-late stage, the old oilfields still occupy a dominant position in the production structure. The seepage process of reservoirs in the high Water Content Period (WCP) presents significant nonlinear and non-homogeneous evolution characteristics, and the traditional seepage-modeling methods are facing the double challenges of accuracy and adaptability when dealing with complex dynamic scenarios. In recent years, Deep Learning technology has gradually become an important tool for reservoir seepage field prediction by virtue of its powerful feature extraction and nonlinear modeling capabilities. This paper systematically reviews the development history of seepage field prediction methods and focuses on the typical models and application paths of Deep Learning in this field, including FeedForward Neural networks, Convolutional Neural Networks, temporal networks, Graphical Neural Networks, and Physical Information Neural Networks (PINNs). Key processes based on Deep Learning, such as feature engineering, network structure design, and physical constraint integration mechanisms, are further explored. Based on the summary of the existing results, this paper proposes future development directions including real-time prediction and closed-loop optimization, multi-source data fusion, physical consistency modeling and interpretability enhancement, model migration, and online updating capability. The research aims to provide theoretical support and technical reference for the intelligent development of old oilfields, the construction of digital twin reservoirs, and the prediction of seepage behavior in complex reservoirs. Full article

The presence and biological role of extracellular DNA (eDNA) have been explored in diverse microbial environments. Nonetheless, it has not been studied in the chicken gut microbiome. This study aims to investigate eDNA in the chicken cecum, analyzing cecal samples from broiler chickens using three preparation methods: Whole Cecal Suspension (WCS), Washed Cell Pellets (WCP), and Cell-Free Supernatant (CFS). The 16S rRNA gene-based microbiota analysis revealed distinct microbial communities in CFS compared to WCS and WCP (p = 0.001). Notably, specific taxa, including Anaerofilum, Anaerotruncus, Oscillospira, Syntrophomonas, and Delftia, were enriched in CFS. Confocal fluorescence microscopy, employing stains such as Propidium Iodide (PI), GelGreen, and SYTO 9, confirmed the presence of eDNA with filaments observed in WCS and CFS. Colocalization of PI and GelGreen™ validated the extracellular nature of eDNA, while DNase I treatment selectively degraded eDNA, further confirming its extracellular nature. Our findings in this study highlight the presence of eDNA in the chicken cecal microbiome, and the presence of eDNA associated with specific taxonomic groups suggest that it might play a specific role in the biological function of the cecal microbiome, which warrants further investigation in the future. Full article