Search Results (1,376)

Objectives: Early death after trauma has been described several times. Little is known about it after traumatic brain injury (TBI) and decompressive craniectomy (DC). The aim of this study was to characterize patients who die after a TBI and DC during their in-hospital stay. Methods: In a subgroup analysis of a retrospective, multicenter, and observational study, non-survivors from in-hospital stays treated for severe TBI and DC were included. Propensity score matching (PSM) was used. Results: A total of 223 patients with severe TBI were treated with DC, and there were 65 (29.1%) patients who did not survive. Of these, 22 (33.8%) died within the first 24 h. Non-survivors were older (p = 0.010), and pupillomotor dysfunction and a higher heart rate on admission were more common (p < 0.001). PSM patients for overall survival (41, 18.4%) differed in mean heart rate from the deceased (p = 0.030). In a multivariate model, age (OR: 1.045, p = 0.013, CI95%: 1.010 to 1.082), Quick value (OR: 0.965, p = 0.049, CI95%: 0.931 to 1.000), and heart rate (OR: 1.099, p = 0.030, CI95%: 1.009 to 1.197) were confirmed as predictive factors. Conclusions: Even after DC, known factors, such as chronological age and comorbidities, have a significant influence on mortality. The value of DC in an aging society for a particular severity of TBI should be further assessed on the basis of prospective studies. Full article

We report on the welding of optical borosilicate glass to an unpolished copper substrate (surface Ra of 0.27 µm and Rz of 1.89 µm) using bursts of femtosecond laser pulses. The present paper puts forth the hypothesis that glass–metal welding with a gap is contingent upon the ejection of molten jets of glass. We have ascertained the impact of pulse energy and focal position on weldability. This finding serves to substantiate our initial hypothesis and provides a framework for understanding the conditions under which this hypothesis is applicable. Under optimal conditions, but without the assistance of any clamping system, our welded samples maintained a breaking resistance of up to 10.9 MPa. Full article

Lotus corniculatus L. is a valuable fodder legume, recognized for its ecological adaptability and high potential for production and fodder quality. In this study, 18 genotypes collected from wild flora were analyzed to highlight genetic variability and facilitate the selection of genotypes with superior potential. The collection area was in the western part of Romania and featured a diverse topography, including parts of the Banat Plain, the Banat Hills, and the Southern and Western Carpathians. The genotypes selected from the wild flora were cultivated and evaluated for morpho-productive and forage quality traits, including pod weight, average number of seeds/pods, green mass production, and protein percentage. PCA highlighted the main components explaining the variability, and K-means clustering allowed for the identification of groups of genotypes with similar performances. ANOVA showed statistically significant differences (p < 0.001) for all traits analyzed. According to the results, genotypes LV-LC-3, LV-LC-4, LV-LC-6, and LV-LC-16 showed high productive potential and were highlighted as the most valuable for advancing in the breeding program. The moderate relationships between traits confirm the importance of integrated selection. The identified genetic variability and selected genotypes support the implementation of effective breeding strategies to obtain high-performance Lotus corniculatus L., adapted to local soil and climate conditions and with a superior forage yield. Full article

A multidisciplinary study was conducted to reconstruct the paleoenvironmental evolution of Maladroxia Bay, one of the principal bays of the islet of Sant’Antioco in southwestern Sardinia, over the past eight millennia. As part of an archaeological landscape project, this study explores the paleogeography and environment of the bay from a diachronic perspective to gain insights into the Holocene relative sea level history, shoreline displacements, and the environmental conditions during different phases. This study is based on an analysis of four sediment cores in conjunction with a chronological model that is based on radiocarbon dates. Four relative sea level indicators were produced. These are the first such indicators from the early and middle Holocene for the island of Sant’Antioco. The results indicate that in the early Holocene, the area was a terrestrial, fluvial environment without marine influence. In the 6th millennium BCE, the rising sea level and marine transgression resulted in the formation of a shallow inner lagoon. It reached its maximum extent in the middle of the 5th millennium BCE. Afterwards, a gradual transition from lagoon to floodplain, and a seaward shift of the shoreline occurred. The lagoon potentially served as a valuable source of food and resources during the middle Holocene. During the Nuragic period (2nd to 1st millennium BCE), the Bay of Maladroxia was very similar to how it is today. Its location was ideal for use as an anchorage, due to the calm and sheltered conditions that prevailed. Full article

Dams play a vital role in securing water and electricity supplies for households and industry, and they contribute significantly to flood protection. Regular monitoring of dam deformations holds fundamental socio-economic and ecological importance. Traditionally, this has relied on time-consuming in situ techniques that offer either high spatial or temporal resolution. Persistent Scatterer Interferometry (PSI) addresses these limitations, enabling high-resolution monitoring in both domains. Sensors such as TerraSAR-X (TSX) and Sentinel-1 (S-1) have proven effective for deformation analysis with millimeter accuracy. Combining TSX and S-1 datasets enhances monitoring capabilities by leveraging the high spatial resolution of TSX with the broad coverage of S-1. This improves monitoring by increasing PS point density, reducing revisit intervals, and facilitating the detection of environmental deformation drivers. This study aims to investigate two objectives: first, we evaluate the benefits of a spatially and temporally densified PS time series derived from TSX and S-1 data for detecting radial deformations in individual dam segments. To support this, we developed the TSX2StaMPS toolbox, integrated into the updated snap2stamps workflow for generating single-master interferogram stacks using TSX data. Second, we identify deformation drivers using water level and temperature as exogenous variables. The five-year study period (2017–2022) was conducted on a gravity dam in North Rhine-Westphalia, Germany, which was divided into logically connected segments. The results were compared to in situ data obtained from pendulum measurements. Linear models demonstrated a fair agreement between the combined time series and the pendulum data ($R^2$ = 0.5; MAE = 2.3 mm). Temperature was identified as the primary long-term driver of periodic deformations of the gravity dam. Following the filling of the reservoir, the variance in the PS data increased from 0.9 mm to 3.9 mm in RMSE, suggesting that water level changes are more responsible for short-term variations in the SAR signal. Upon full impoundment, the mean deformation amplitude decreased by approximately 1.7 mm toward the downstream side of the dam, which was attributed to the higher water pressure. The last five meters of water level rise resulted in higher feature importance due to interaction effects with temperature. The study concludes that integrating multiple PS datasets for dam monitoring is beneficial particularly for dams where few PS points can be identified using one sensor or where pendulum systems are not installed. Identifying the drivers of deformation is feasible and can be incorporated into existing monitoring frameworks. Full article

In this work, the synthesis and structural characterization of the smallest possible member of the family of bis-functionalized {MnMo₆O₂₄} Anderson–Evans polyoxometalates (POMs) is reported. The synthesis of the title compound TBA₃{[HC(CH₂O)₃]₂MnMo₆O₁₈} (1) was accomplished by using trimethylolmethane as the capping unit (TBA: tetra(n-butyl)ammonium, n-B_u4N⁺). The molecular structure of the organic–inorganic POM gave rise to yet undisclosed ¹H-NMR features, which are discussed thoroughly. Single-crystal X-ray diffraction (XRD) analysis revealed a highly regular 3D packing of the polyoxoanions within a matrix of TBA cations. The hybrid POM is of particular interest regarding potential applications in photocatalysis (i.e., hydrogen evolution) and energy storage. Thus, the electrochemical and thermal properties of 1 are also analyzed. Full article

Herpes simplex virus type 1 (HSV-1) has a global prevalence of 64%. Established antiviral drugs, such as acyclovir (ACV), have been successfully used over the past decades. However, due to growing viral resistance against approved antivirals and the lack of effective vaccines, new concepts are essential to target HSV-1 infections. Here, we present data on the inhibitory effect of the procaine-based substance ProcCluster^® (PC) in reducing HSV-1 replication in vitro. Non-toxic PC concentrations significantly decreased HSV-1 replication in infected cells. Immunofluorescence microscopy revealed an accumulation of viral proteins in early and recycling endosomes, resulting in reduced viral release. The combination of PC with ACV resulted in an enhanced antiviral effect. Based on these results, PC alone, as well as in combination with ACV, appears to be a promising substance with antiviral potential against HSV-1 infections. Full article

Background: Neurodevelopmental disorders (NDDs), including autism spectrum disorder (ASD), are genetically complex and often linked to structural genomic variations such as copy number variants (CNVs). Current diagnostic strategies face challenges in interpreting the clinical significance of such variants. Methods: We developed a customized, gene-oriented chromosomal microarray (CMA) targeting 6026 genes relevant to neurodevelopment, aiming to improve diagnostic yield and candidate gene prioritization. A total of 39 patients with unexplained developmental delay, intellectual disability, and/or ASD were analyzed using this custom platform. Systems biology approaches were employed for downstream interpretation, including protein–protein interaction networks, centrality measures, and tissue-specific functional module analysis. Results: Pathogenic or likely pathogenic CNVs were identified in 31% of cases (9/29). Network analyses revealed candidate genes with key topological properties, including central “hubs” (e.g., NPEPPS, PSMG1, DOCK8) and regulatory “bottlenecks” (e.g., SLC15A4, GLT1D1, TMEM132C). Tissue- and cell-type-specific network modeling demonstrated widespread gene involvement in both prenatal and postnatal developmental modules, with glial and astrocytic networks showing notable enrichment. Several novel CNV regions with high pathogenic potential were identified and linked to neurodevelopmental phenotypes in individual patient cases. Conclusions: Customized CMA offers enhanced detection of clinically relevant CNVs and provides a framework for prioritizing novel candidate genes based on biological network integration. This approach improves diagnostic accuracy in NDDs and identifies new targets for future functional and translational studies, highlighting the importance of glial involvement and immune-related pathways in neurodevelopmental pathology. Full article

This paper presents a meta-analysis of social research on water, offering a novel methodological contribution to the study of emerging interdisciplinary research fields. We propose and implement a mixed methods framework that integrates quantitative network analysis with qualitative research, aiming to enhance both to give access to new emerging empirical fields and enhance the analytical depth of empirical social research. Drawing on a dataset of publications from the Web of Science over four distinct time intervals, we identify thematic clusters through keyword co-occurrence networks that reveal the evolving structure and internal dynamics of the field. Our findings show a clear trend toward increasing interdisciplinarity, responsiveness to global events, and contemporary challenges such as the emergence of COVID-19 and the continued centrality of topics related to water management and evaluation. By uncovering latent structures, our approach not only maps the field’s development but also lays the foundation for targeted qualitative analysis of articles representative of identified clusters. This methodological design contributes to the broader discourse on mixed methods research in the social sciences by demonstrating how computational tools can enhance the transparency and reliability of qualitative inquiry without sacrificing its interpretive richness. Furthermore, this study opens new avenues for critically reflecting on the epistemic culture of social water research, particularly in relation to its proximity to applied science and governance-oriented perspectives. The proposed method holds potential relevance for both academic researchers and decision makers in the water sector, offering a means to systematically access dispersed knowledge and identify underrepresented subfields. Overall, the study showcases the potential of mixed methods designs for navigating and structuring complex interdisciplinary research landscapes. Full article

Azole resistance in dermatophytes, particularly Trichophyton indotineae, has become a growing global concern. Current antifungal susceptibility testing protocols (EUCAST, CLSI) have limitations in reproducibility and sensitivity. This study aimed to evaluate how medium composition, incubation temperature, and spore concentration influence itraconazole susceptibility testing across various dermatophyte species. Thirty-eight clinical isolates representing Trichophyton, Microsporum, and Epidermophyton species were tested using a microplate laser nephelometry system (MLN). IC₅₀ values for itraconazole were determined in three different media (Sabouraud glucose (SG), RPMI-based (RG), and RG supplemented with casein (RGC)) at 28 °C and 34 °C. Effects of spore concentration on growth dynamics and lag phase were also analyzed. SG medium provided clear phenotypic separation between resistant and sensitive isolates. In contrast, RG and RGC showed overlapping IC₅₀ values. Lower spore concentrations revealed underlying growth differences, which were masked at higher inoculum levels. Temperature and media composition significantly affected IC₅₀ outcomes. Genotypic analysis confirmed resistance-associated Erg11B point mutations and genomic amplifications in T. indotineae, particularly in combination with Erg1 mutations, forming distinct subpopulations. SG medium combined with reduced spore concentrations offered improved differentiation of resistant versus sensitive strains. These findings support the development of more accurate susceptibility testing protocols and highlight the need to establish species-specific ECOFF values for dermatophytes. Full article

Objectives: The aim of this study was to assess the association of diabetes mellitus and its medications with overall response (ORR) and mortality or cancer-specific survival (CSS) in patients with metastatic urothelial cancer receiving enfortumab vedotin monotherapy. Methods: This multicentre retrospective cohort study was designed according to the guidelines for the synthesis of qualitative research (ENTREQ). Eligible patients were adults (≥18) years treated with enfortumab vedotin monotherapy for metastatic urothelial cancer between June 2024 and January 2025. A total of 125 patients were reported across 11 centres. Results: The cohort included 93 males (74.4%) and 32 females (25.6%), with a mean age of 68.3 years (SD 9.3). The primary tumour site was the bladder in 109 (87.2%) cases and the upper tract (UTUC) in 16 (12.8%) cases. Interestingly, medication with metformin was significantly associated with cancer-specific mortality (37.9% versus 77.8%; p = 0.019), while patients with insulin-dependent diabetes mellitus had a significantly better CSS (Log Rank = 0.004). Upon comparing only patients who already had diabetes mellitus and then received anti-diabetic medication, there was a significant association between patients with diabetes mellitus receiving metformin and a worse 3-month ORR (80.0% versus 55.6%; p = 0.039). Regarding the subpopulation of UTUC, cancer-specific mortality was significantly associated with metformin medication (p = 0.033). Conclusions: Despite recent reports that metformin has protective effects in urothelial cancer, our findings suggest that metformin use may be linked to worse responses and survival outcomes in patients treated with enfortumab vedotin monotherapy. Further research, particularly translational research into the underlying diabetic and pharmacologic pathways, is warranted. Full article

A branch of the nucleotide excision repair (NER) pathway, transcription-coupled repair (TCR or TC-NER) specifically operates on the template DNA strand of actively transcribed genes. Initiated by stalling of elongating RNA polymerase complexes at damaged sites, TC-NER has historically been viewed as “accelerated repair”, arguably necessary for the maintenance of vital transcription function. Conversely, the conventional “global genome” (GG-NER) mechanism, operating throughout the genome, is usually regarded as a much slower process, even though it has long been found that differences in repair kinetics between transcribed DNA and the rest of the genome are not manifested for all structural types of DNA damage. Considering that damage detection is the rate-limiting step of overall repair reactions in most cases and that the mechanisms of the initial recognition of modified DNA structure are fundamentally different between TC-NER and GG-NER, it is suggestive to attribute the observed kinetic differences to different damage spectra recognized by the two pathways. This review summarizes current knowledge on the differential requirements of TC-NER and GG-NER towards specific damage types, based on their structural rather than spatial characteristics, and highlights some common features of DNA modifications repaired preferentially or exclusively by TC-NER, while evading other repair mechanisms. Full article

With the emergence of a new vitamin concept—vitamin A5—it is essential to first clarify the basic definition of vitamins, particularly vitamin A. This article summarizes the foundational concepts and definitions of vitamins with particular relevance to the discovery, establishment, and categorization of new vitamin concepts. Vitamin A5 was discovered 80 years after the last vitamin was identified. It serves as an umbrella term for the dietary precursors 9-cis-β,β-carotene and 9-cis-13,14-dihydroretinol for the endogenous activator of the nuclear hormone receptor RXR, 9-cis-13,14-dihydroretinoic acid. However, several questions arise: Which criteria are typically used to identify a substance as a vitamin? How does vitamin A5 fit into the sometimes misleading definition of vitamin A? This review summarizes key findings and provides a comprehensive assessment of the current understanding, concluding that (a) vitamin A5 is a newly identified micronutrient that plays an important role in the prevention of diet-related diseases and (b) vitamin A5 is an important micronutrient that provides a plausible, mechanistic explanation for why a Western lifestyle diet low in vegetables and especially leafy vegetables can lead to a high prevalence of Western-lifestyle diseases, particularly neurological diseases and poor mental health. Full article

Duckweeds are aquatic monocotyledonous plants known to be the smallest and the fastest growing angiosperms. The 7th International Conference on Duckweed Research and Applications (7th ICDRA) was held in Bangkok, Thailand, from 12th to 16th November 2024. The conference drew young and experienced scientists from across the world who presented their research in varied fields. This conference report presents the highlights of the advancements in the field of duckweed research and application in the sections: Genomics and Cell Biology; Diversity, Ecology, Evolution; Physiology, Reproduction, Metabolomics; Microbiome and Interactions; Applications; and Future Outlook. The next conference, 8th ICDRA, will be held in Naples, Italy, in 2026. Full article

The Adaptive Skip-gram (AdaGram) algorithm extends traditional word embeddings by learning multiple vector representations per word, enabling the capture of contextual meanings and polysemy. Originally implemented in Julia, AdaGram has seen limited adoption due to ecosystem fragmentation and the comparative scarcity of Julia’s machine learning tooling compared to Python’s mature frameworks. In this work, we present a Python-based reimplementation of AdaGram that facilitates broader integration with modern machine learning tools. Our implementation expands the model’s applicability beyond natural language, enabling the analysis of scientific notation—particularly chemical and physical formulas encoded in LaTeX. We detail the algorithmic foundations, preprocessing pipeline, and hyperparameter configurations needed for interdisciplinary corpora. Evaluations on real-world texts and LaTeX-encoded formulas demonstrate AdaGram’s effectiveness in unsupervised word sense disambiguation. Comparative analyses highlight the importance of corpus design and parameter tuning. This implementation opens new applications in formula-aware literature search engines, ambiguity reduction in automated scientific summarization, and cross-disciplinary concept alignment. Full article