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23 pages, 3580 KiB  
Article
Distributed Collaborative Data Processing Framework for Unmanned Platforms Based on Federated Edge Intelligence
by Siyang Liu, Nanliang Shan, Xianqiang Bao and Xinghua Xu
Sensors 2025, 25(15), 4752; https://doi.org/10.3390/s25154752 (registering DOI) - 1 Aug 2025
Abstract
Unmanned platforms such as unmanned aerial vehicles, unmanned ground vehicles, and autonomous underwater vehicles often face challenges of data, device, and model heterogeneity when performing collaborative data processing tasks. Existing research does not simultaneously address issues from these three aspects. To address this [...] Read more.
Unmanned platforms such as unmanned aerial vehicles, unmanned ground vehicles, and autonomous underwater vehicles often face challenges of data, device, and model heterogeneity when performing collaborative data processing tasks. Existing research does not simultaneously address issues from these three aspects. To address this issue, this study designs an unmanned platform cluster architecture inspired by the cloud-edge-end model. This architecture integrates federated learning for privacy protection, leverages the advantages of distributed model training, and utilizes edge computing’s near-source data processing capabilities. Additionally, this paper proposes a federated edge intelligence method (DSIA-FEI), which comprises two key components. Based on traditional federated learning, a data sharing mechanism is introduced, in which data is extracted from edge-side platforms and placed into a data sharing platform to form a public dataset. At the beginning of model training, random sampling is conducted from the public dataset and distributed to each unmanned platform, so as to mitigate the impact of data distribution heterogeneity and class imbalance during collaborative data processing in unmanned platforms. Moreover, an intelligent model aggregation strategy based on similarity measurement and loss gradient is developed. This strategy maps heterogeneous model parameters to a unified space via hierarchical parameter alignment, and evaluates the similarity between local and global models of edge devices in real-time, along with the loss gradient, to select the optimal model for global aggregation, reducing the influence of device and model heterogeneity on cooperative learning of unmanned platform swarms. This study carried out extensive validation on multiple datasets, and the experimental results showed that the accuracy of the DSIA-FEI proposed in this paper reaches 0.91, 0.91, 0.88, and 0.87 on the FEMNIST, FEAIR, EuroSAT, and RSSCN7 datasets, respectively, which is more than 10% higher than the baseline method. In addition, the number of communication rounds is reduced by more than 40%, which is better than the existing mainstream methods, and the effectiveness of the proposed method is verified. Full article
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19 pages, 3457 KiB  
Article
Impaired Mitochondrial DNA Copy Number in Visceral Adipose Tissue of Insulin-Resistant Individuals: Implications for Metabolic Dysregulation
by Monika Ołdakowska, Aneta Cierzniak, Tomasz Jurek and Małgorzata Małodobra-Mazur
Int. J. Mol. Sci. 2025, 26(15), 7398; https://doi.org/10.3390/ijms26157398 (registering DOI) - 31 Jul 2025
Viewed by 51
Abstract
Insulin resistance is a fundamental pathophysiological mechanism contributing to the development of type 2 diabetes and metabolic syndrome. Recently, attention has focused on mitochondria’s role in glucose and lipid metabolism. Mitochondrial dysfunction is strongly associated with impaired energy metabolism and elevated oxidative stress. [...] Read more.
Insulin resistance is a fundamental pathophysiological mechanism contributing to the development of type 2 diabetes and metabolic syndrome. Recently, attention has focused on mitochondria’s role in glucose and lipid metabolism. Mitochondrial dysfunction is strongly associated with impaired energy metabolism and elevated oxidative stress. We investigated the mitochondrial DNA (mtDNA) copy number in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) in insulin-sensitive (IS) and insulin-resistant (IR) individuals. Twenty-seven paired adipose tissue biopsies were obtained during elective abdominal surgery. DNA and RNA were extracted, and mtDNA copy number was quantified using Real-Time PCR. We found that mtDNA content in VAT was approximately two-fold lower than in SAT. Furthermore, in IR individuals, mtDNA copy number was significantly reduced in both SAT and VAT compared to IS subjects. A strong positive correlation was observed between mtDNA content in VAT and body mass index (BMI), and a negative correlation was found with the QUICKI index. Additionally, mtDNA copy number in VAT positively correlated with the expression of several genes involved in insulin signalling, lipid metabolism, and other metabolic pathways. These findings underscore the central role of mitochondrial function in VAT in the context of metabolic disorders and suggest that targeting mitochondrial regulation in this tissue may represent a promising therapeutic approach. Full article
(This article belongs to the Special Issue Mitochondrial Function in Human Health and Disease: 2nd Edition)
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17 pages, 3919 KiB  
Article
On the Links Between Tropical Sea Level and Surface Air Temperature in Middle and High Latitudes
by Sergei Soldatenko, Genrikh Alekseev and Yaromir Angudovich
Atmosphere 2025, 16(8), 913; https://doi.org/10.3390/atmos16080913 - 28 Jul 2025
Viewed by 135
Abstract
Change in sea level (SL) is an important indicator of global warming, since it reflects alterations in several components of the climate system at once. The main factors behind this phenomenon are the melting of glaciers and thermal expansion of ocean water, with [...] Read more.
Change in sea level (SL) is an important indicator of global warming, since it reflects alterations in several components of the climate system at once. The main factors behind this phenomenon are the melting of glaciers and thermal expansion of ocean water, with the latter contributing about 40% to the overall rise in SL. Rising SL indirectly indicates an increase in ocean heat content and, consequently, its surface temperature. Previous studies have found that tropical sea surface temperature (SST) is critical to regulating the Earth’s climate and weather patterns in high and mid-latitudes. For this reason, SST and SL in the tropics can be considered as precursors of both global climate change and the emergence of climate anomalies in extratropical latitudes. Although SST has been used in this capacity in a number of studies, similar research regarding SL had not been conducted until recently. In this paper, we examine the links between SL in the tropical North Atlantic and North Pacific Oceans and surface air temperature (SAT) at mid- and high latitudes, with the aim of assessing the potential of SL as a predictor in forecasting SAT anomalies. To identify similarities between the variability of tropical SL and SST and that of SAT in high- and mid-latitude regions, as well as to estimate possible time lags, we applied factor analysis, clustering, cross-correlation and cross-spectral analyses. The results reveal a structural similarity in the internal variability of tropical SL and extratropical SAT, along with a significant lagged relationship between them, with a time lag of several years. Full article
(This article belongs to the Section Climatology)
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29 pages, 2830 KiB  
Article
BCINetV1: Integrating Temporal and Spectral Focus Through a Novel Convolutional Attention Architecture for MI EEG Decoding
by Muhammad Zulkifal Aziz, Xiaojun Yu, Xinran Guo, Xinming He, Binwen Huang and Zeming Fan
Sensors 2025, 25(15), 4657; https://doi.org/10.3390/s25154657 - 27 Jul 2025
Viewed by 320
Abstract
Motor imagery (MI) electroencephalograms (EEGs) are pivotal cortical potentials reflecting cortical activity during imagined motor actions, widely leveraged for brain-computer interface (BCI) system development. However, effectively decoding these MI EEG signals is often overshadowed by flawed methods in signal processing, deep learning methods [...] Read more.
Motor imagery (MI) electroencephalograms (EEGs) are pivotal cortical potentials reflecting cortical activity during imagined motor actions, widely leveraged for brain-computer interface (BCI) system development. However, effectively decoding these MI EEG signals is often overshadowed by flawed methods in signal processing, deep learning methods that are clinically unexplained, and highly inconsistent performance across different datasets. We propose BCINetV1, a new framework for MI EEG decoding to address the aforementioned challenges. The BCINetV1 utilizes three innovative components: a temporal convolution-based attention block (T-CAB) and a spectral convolution-based attention block (S-CAB), both driven by a new convolutional self-attention (ConvSAT) mechanism to identify key non-stationary temporal and spectral patterns in the EEG signals. Lastly, a squeeze-and-excitation block (SEB) intelligently combines those identified tempo-spectral features for accurate, stable, and contextually aware MI EEG classification. Evaluated upon four diverse datasets containing 69 participants, BCINetV1 consistently achieved the highest average accuracies of 98.6% (Dataset 1), 96.6% (Dataset 2), 96.9% (Dataset 3), and 98.4% (Dataset 4). This research demonstrates that BCINetV1 is computationally efficient, extracts clinically vital markers, effectively handles the non-stationarity of EEG data, and shows a clear advantage over existing methods, marking a significant step forward for practical BCI applications. Full article
(This article belongs to the Special Issue Advanced Biomedical Imaging and Signal Processing)
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8 pages, 2843 KiB  
Proceeding Paper
Coastal Erosion in Tsunami and Storm Surges-Exposed Areas in Licantén, Maule, Chile: Case Study Using Remote Sensing and In-Situ Data
by Joaquín Valenzuela-Jara, Idania Briceño de Urbaneja, Waldo Pérez-Martínez and Isidora Díaz-Quijada
Eng. Proc. 2025, 94(1), 10; https://doi.org/10.3390/engproc2025094010 - 24 Jul 2025
Viewed by 257
Abstract
This study examines urban expansion, coastal erosion, and extreme wave events in Licantén, Maule Region, following the 2010 earthquake and tsunami. Using multi-source data—Landsat and Sentinel-2 imagery, ERA5 reanalysis, high-resolution Maxar images, UAV surveys, and the CoastSat algorithm—we detected significant urban growth in [...] Read more.
This study examines urban expansion, coastal erosion, and extreme wave events in Licantén, Maule Region, following the 2010 earthquake and tsunami. Using multi-source data—Landsat and Sentinel-2 imagery, ERA5 reanalysis, high-resolution Maxar images, UAV surveys, and the CoastSat algorithm—we detected significant urban growth in tsunami-prone areas: Iloca (36.88%), La Pesca (33.34%), and Pichibudi (20.78%). A 39-year shoreline reconstruction (1985–2024) revealed notable changes in erosion rates and shoreline dynamics using DSAS v6.0, influenced by tides, storm surges, and wave action modeled in R to quantify storm surge events over time. Results underscore the lack of urban planning in hazard-exposed areas and the urgent need for resilient coastal management under climate change. Full article
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18 pages, 8466 KiB  
Article
COTS Battery Charge Equalizer for Small Satellite Applications
by Pablo Casado, José M. Blanes, Ausiàs Garrigós, David Marroquí and Cristian Torres
Appl. Sci. 2025, 15(15), 8228; https://doi.org/10.3390/app15158228 - 24 Jul 2025
Viewed by 148
Abstract
This paper describes the design and implementation of a battery equalizer circuit for small satellites, developed under the New Space philosophy exclusively using commercial off-the-shelf (COTS) components. The primary objective is to ensure high reliability for mission-critical power systems while adhering to strict [...] Read more.
This paper describes the design and implementation of a battery equalizer circuit for small satellites, developed under the New Space philosophy exclusively using commercial off-the-shelf (COTS) components. The primary objective is to ensure high reliability for mission-critical power systems while adhering to strict cost constraints. In order to achieve this objective, the design incorporates a robust analog control circuit, thereby avoiding the complexities and potential single-point failures associated with digital controllers. A comprehensive study of various cell-balancing topologies was conducted, leading to the selection, hardware implementation, and comparative analysis of the two most suitable candidates. The results of this study provide a validated, cost-effective, and reliable battery equalizer solution for developers of small satellites. Full article
(This article belongs to the Special Issue Control Systems for Next Generation Electric Applications)
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28 pages, 8337 KiB  
Article
Collision Detection Algorithms for Autonomous Loading Operations of LHD-Truck Systems in Unstructured Underground Mining Environments
by Mingyu Lei, Pingan Peng, Liguan Wang, Yongchun Liu, Ru Lei, Chaowei Zhang, Yongqing Zhang and Ya Liu
Mathematics 2025, 13(15), 2359; https://doi.org/10.3390/math13152359 - 23 Jul 2025
Viewed by 201
Abstract
This study addresses collision detection in the unmanned loading of ore from load-haul-dump (LHD) machines into mining trucks in underground metal mines. Such environments present challenges like heavy dust, confined spaces, sensor occlusions, and poor lighting. This work identifies two primary collision risks [...] Read more.
This study addresses collision detection in the unmanned loading of ore from load-haul-dump (LHD) machines into mining trucks in underground metal mines. Such environments present challenges like heavy dust, confined spaces, sensor occlusions, and poor lighting. This work identifies two primary collision risks and proposes corresponding detection strategies. First, for collisions between the bucket and tunnel walls, LiDAR is used to collect 3D point cloud data. The point cloud is processed through filtering, downsampling, clustering, and segmentation to isolate the bucket and tunnel wall. A KD-tree algorithm is then used to compute distances to assess collision risk. Second, for collisions between the bucket and the mining truck, a kinematic model of the LHD’s working device is established using the Denavit–Hartenberg (DH) method. Combined with inclination sensor data and geometric parameters, a formula is derived to calculate the pose of the bucket’s tip. Key points from the bucket and truck are then extracted to perform collision detection using the oriented bounding box (OBB) and the separating axis theorem (SAT). Simulation results confirm that the derived pose estimation formula yields a maximum error of 0.0252 m, and both collision detection algorithms demonstrate robust performance. Full article
(This article belongs to the Special Issue Mathematical Modeling and Analysis in Mining Engineering)
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22 pages, 2422 KiB  
Article
OSIRIS4CubeSat—The World’s Smallest Commercially Available Laser Communication Terminal
by Benjamin Rödiger, Christian Roubal, Fabian Rein, René Rüddenklau, Anil Morab Vishwanath and Christopher Schmidt
Aerospace 2025, 12(8), 655; https://doi.org/10.3390/aerospace12080655 - 23 Jul 2025
Viewed by 182
Abstract
The New Space movement led to an exponential increase in the number of the smallest satellites in orbit in the last two decades. The number of required communication channels increased with that as well and revealed the limitations of classical radio frequency channels. [...] Read more.
The New Space movement led to an exponential increase in the number of the smallest satellites in orbit in the last two decades. The number of required communication channels increased with that as well and revealed the limitations of classical radio frequency channels. Free-space optical communication overcomes these challenges and has been successfully demonstrated, with operational systems in orbit on large and small satellites. The next step is to miniaturize the technology of laser communication to make it usable on CubeSats. Thus, the German Aerospace Center (DLR) developed, together with Tesat-Spacecom GmbH & Co. KG in Backnang, Germany, a highly miniaturized and power-efficient laser terminal, which is based on a potential customer’s use case. OSIRIS4CubeSat uses a new patented design that combines electronics and optomechanics into a single system architecture to achieve a high compactness following the CubeSat standard. Interfaces and software protocols that follow established standards allowed for an easy transition to the industry for a commercial mass market. The successful demonstration of OSIRIS4CubeSat during the PIXL-1 mission proved its capabilities and the advantages of free-space optical communication in the final environment. This paper gives an overview of the system architecture and the development of the single subsystems. The system’s capabilities are verified by the already published in-orbit demonstration results. Full article
(This article belongs to the Special Issue On-Board Systems Design for Aerospace Vehicles (2nd Edition))
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41 pages, 1710 KiB  
Article
Toward Integrated Satellite Operations and Network Management: A Review and Novel Framework
by Arnau Singla, Franco Criscola, David Canales, Juan A. Fraire, Anna Calveras and Joan A. Ruiz-de-Azua
Technologies 2025, 13(8), 312; https://doi.org/10.3390/technologies13080312 - 22 Jul 2025
Viewed by 353
Abstract
Achieving global coverage and performance goals for 6G requires seamless integration of satellite and terrestrial networks, yet current operational frameworks lack common standards for managing these heterogeneous infrastructures. This paper addresses the critical need for unified satellite-terrestrial network operations by proposing the CMS [...] Read more.
Achieving global coverage and performance goals for 6G requires seamless integration of satellite and terrestrial networks, yet current operational frameworks lack common standards for managing these heterogeneous infrastructures. This paper addresses the critical need for unified satellite-terrestrial network operations by proposing the CMS framework, a novel task-scheduling-based approach that bridges the operational gap between satellite operations (SatOps) and network operations (NetOps). The framework integrates satellite-specific constraints with network service requirements and QoS metrics through constraint satisfaction programming and multi-objective optimization. Three novel architectures are introduced: integrated operations (embedding NetOps within SatOps), coordinated operations (unified control with separate execution channels), and adaptive operations (mutual adaptation through intelligent interfaces). Each architecture addresses different connectivity scenarios and integration requirements for both sporadic and persistent satellite constellations. The proposed architectures are evaluated against challenges spanning infrastructure and architecture, interoperability and standardization, integrated management, operational dynamics, and technology maturation and deployment. Validation through simulation demonstrates significant performance improvements, with task completion rates improving by 17.87% to 44.02% and data throughput gains of 25.09% to 93.62% compared to traditional approaches. The CMS framework establishes a resilient operational standard for future 6G networks, offering practical solutions to bridge the current divide between satellite and terrestrial network operations. Full article
(This article belongs to the Section Information and Communication Technologies)
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18 pages, 4936 KiB  
Review
The Small Frontier: Trends Toward Miniaturization and the Future of Planetary Surface Rovers
by Carrington Chun, Faysal Chowdoury, Muhammad Hassan Tanveer, Sumit Chakravarty and David A. Guerra-Zubiaga
Actuators 2025, 14(7), 356; https://doi.org/10.3390/act14070356 - 20 Jul 2025
Viewed by 429
Abstract
The robotic exploration of space began only five decades ago, and yet in the intervening years, a wide and diverse ecosystem of robotic explorers has been developed for this purpose. Such devices have greatly benefited from miniaturization trends and the increased availability of [...] Read more.
The robotic exploration of space began only five decades ago, and yet in the intervening years, a wide and diverse ecosystem of robotic explorers has been developed for this purpose. Such devices have greatly benefited from miniaturization trends and the increased availability of high-quality commercial off-the-shelf (COTS) components. This review outlines the specific taxonomic distinction between planetary surface rovers and other robotic space exploration vehicles, such as orbiters and landers. Additionally, arguments are made to standardize the classification of planetary rovers by mass into categories similar to those used for orbital satellites. Discussions about recent noteworthy trends toward the miniaturization of planetary rovers are also included, as well as a compilation of previous planetary rovers. This analysis compiles relevant metrics such as the mass, the distance traveled, and the locomotion or actuation technique for previous planetary rovers. Additional details are also examined about archetypal rovers that were chosen as representatives of specific small-scale rover classes. Finally, potential future trends for miniature planetary surface rovers are examined by way of comparison to similar miniaturized orbital robotic explorers known as CubeSats. Based on the existing relationship between CubeSats and their Earth-based simulation equivalents, CanSats, the importance of a potential Earth-based analog for miniature rovers is identified. This research establishes such a device, coining the new term ‘CanBot’ to refer to pathfinding systems that are deployed terrestrially to help develop future planetary surface exploration robots. Establishing this explicit genre of robotic vehicle is intended to provide a unified means for categorizing and encouraging the development of future small-scale rovers. Full article
(This article belongs to the Special Issue Feature Papers in Actuators for Surface Vehicles)
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28 pages, 3531 KiB  
Review
Review of Acoustic Emission Detection Technology for Valve Internal Leakage: Mechanisms, Methods, Challenges, and Application Prospects
by Dongjie Zheng, Xing Wang, Lingling Yang, Yunqi Li, Hui Xia, Haochuan Zhang and Xiaomei Xiang
Sensors 2025, 25(14), 4487; https://doi.org/10.3390/s25144487 - 18 Jul 2025
Viewed by 401
Abstract
Internal leakage within the valve body constitutes a severe potential safety hazard in industrial fluid control systems, attributable to its high concealment and the resultant difficulty in detection via conventional methodologies. Acoustic emission (AE) technology, functioning as an efficient non-destructive testing approach, is [...] Read more.
Internal leakage within the valve body constitutes a severe potential safety hazard in industrial fluid control systems, attributable to its high concealment and the resultant difficulty in detection via conventional methodologies. Acoustic emission (AE) technology, functioning as an efficient non-destructive testing approach, is capable of capturing the transient stress waves induced by leakage, thereby furnishing an effective means for the real-time monitoring and quantitative assessment of internal leakage within the valve body. This paper conducts a systematic review of the theoretical foundations, signal-processing methodologies, and the latest research advancements related to the technology for detecting internal leakage in the valve body based on acoustic emission. Firstly, grounded in Lechlier’s acoustic analogy theory, the generation mechanism of acoustic emission signals arising from valve body leakage is elucidated. Secondly, a detailed analysis is conducted on diverse signal processing techniques and their corresponding optimization strategies, encompassing parameter analysis, time–frequency analysis, nonlinear dynamics methods, and intelligent algorithms. Moreover, this paper recapitulates the current challenges encountered by this technology and delineates future research orientations, such as the fusion of multi-modal sensors, the deployment of lightweight deep learning models, and integration with the Internet of Things. This study provides a systematic reference for the engineering application and theoretical development of the acoustic emission-based technology for detecting internal leakage in valves. Full article
(This article belongs to the Topic Advances in Non-Destructive Testing Methods, 3rd Edition)
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20 pages, 1828 KiB  
Article
The Temporal Dynamics of the Impact of Overfishing on the Resilience of the Sarotherodon melanotheron (Rüppel, 1858) Fish Species’ Population in the West African Lake Toho
by Clovis Ayodédji Idossou Hountcheme, Simon Ahouansou Montcho, Hyppolite Agadjihouede and Doru Bănăduc
Fishes 2025, 10(7), 357; https://doi.org/10.3390/fishes10070357 - 18 Jul 2025
Viewed by 180
Abstract
This research investigated the temporal dynamics of the anthropogenic impact of fishing pressure on the resilience of the fish species Sarotherodon melanotheron (Rüppel, 1858) in the African Lake Toho, located in southwest Benin. The sampling and analysis of monthly length frequency data were [...] Read more.
This research investigated the temporal dynamics of the anthropogenic impact of fishing pressure on the resilience of the fish species Sarotherodon melanotheron (Rüppel, 1858) in the African Lake Toho, located in southwest Benin. The sampling and analysis of monthly length frequency data were conducted from April 2002 to March 2003 and from April 2022 to March 2023 using the FAO-ICLARM Stock Assessment Tool (FiSAT II software program (version 1.2.2.). The analysis of the S. melanotheron population in Lake Toho revealed a significantly diminishing resilience potential, reflected mainly in general reductions in both the average size and weight of individuals. There was a notable reduction in the size of Sarotherodon melanotheron individuals caught between 2002–2003 and 2022–2023, reflecting the increased pressure on juvenile size classes. Catches are now concentrated mainly on immature fish, revealing increasing exploitation before sexual maturity is reached. An analysis of maturity stages showed a decrease in the percentage of mature individuals in the catches (69.27% in 2002–2003 compared to 55.07% in 2022–2023) and a reduction in the number of mega-spawners (4.53% in 2002–2003 compared to 1.56% in 2022–2023). Growth parameters revealed a decrease in asymptotic length (from 32.2 cm to 23.8 cm) and longevity (from 9.37 years to 7.89 years), while the growth coefficient slightly increased. The mean size at first capture and optimal size significantly declined, indicating increased juvenile exploitation. The total and natural mortalities increased, whereas the fishing mortality remained stable. The exploitation rate remained high, despite a slight decrease from 0.69 to 0.65. Finally, the declines in the yield per recruit, maximum sustainable yield, and biomass confirm the increasing fishing pressure, leading to growth overfishing, recruitment overfishing, reproductive overfishing, and, last but not least, a decreasing resilience potential. These findings highlight the growing overexploitation of S. melanotheron in Lake Toho, compromising stock renewal, fish population resilience, sustainability, and production while jeopardizing local food safety. Full article
(This article belongs to the Section Biology and Ecology)
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16 pages, 2433 KiB  
Article
A Single-Cell Assessment of Intramuscular and Subcutaneous Adipose Tissue in Beef Cattle
by Mollie M. Green, Hunter R. Ford, Alexandra P. Tegeler, Oscar J. Benitez, Bradley J. Johnson and Clarissa Strieder-Barboza
Agriculture 2025, 15(14), 1545; https://doi.org/10.3390/agriculture15141545 - 18 Jul 2025
Viewed by 1248
Abstract
Deposition of intramuscular fat (IM), also known as marbling, is the deciding factor of beef quality grade in the U.S. Defining molecular mechanisms underlying the differential deposition of adipose tissue in distinct anatomical areas in beef cattle is key to the development of [...] Read more.
Deposition of intramuscular fat (IM), also known as marbling, is the deciding factor of beef quality grade in the U.S. Defining molecular mechanisms underlying the differential deposition of adipose tissue in distinct anatomical areas in beef cattle is key to the development of strategies for marbling enhancement while limiting the accumulation of excessive subcutaneous adipose tissue (SAT). The objective of this exploratory study was to define the IM and SAT transcriptional heterogeneity at the whole tissue and single-nuclei levels in beef steers. Longissimus dorsi muscle samples (9–11th rib) were collected from two finished beef steers at harvest to dissect matched IM and adjacent SAT (backfat). Total RNA from IM and SAT was isolated and sequenced in an Illumina NovaSeq 6000. Nuclei from the same samples were isolated by dounce homogenization, libraries generated with 10× Genomics, and sequenced in an Illumina NovaSeq 6000, followed by analysis via Cell Ranger pipeline and Seurat in RStudio (v4.3.2) By the expression of signature marker genes, single-nuclei RNA sequencing (snRNAseq) analysis identified mature adipocytes (AD; ADIPOQ, LEP), adipose stromal and progenitor cells (ASPC; PDGFRA), endothelial cells (EC; VWF, PECAM1), smooth muscle cells (SMC; NOTCH3, MYL9) and immune cells (IMC; CD163, MRC1). We detected six cell clusters in SAT and nine in IM. Across IM and SAT, AD was the most abundant cell type, followed by ASPC, SMC, and IMC. In SAT, AD made up 50% of the cellular population, followed by ASPC (31%), EC (14%), IMC (1%), and SMC (4%). In IM depot, AD made up 23% of the cellular population, followed by ASPC at 19% of the population, EC at 28%, IMC at 7% and SMC at 12%. The abundance of ASPC and AD was lower in IM vs. SAT, while IMC was increased, suggesting a potential involvement of immune cells on IM deposition. Accordingly, both bulk RNAseq and snRNAseq analyses identified activated pathways of inflammation and metabolic function in IM. These results demonstrate distinct transcriptional cellular heterogeneity between SAT and IM depots in beef steers, which may underly the mechanisms by which fat deposits in each depot. The identification of depot-specific cell populations in IM and SAT via snRNAseq analysis has the potential to reveal target genes for the modulation of fat deposition in beef cattle. Full article
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27 pages, 6050 KiB  
Article
A Cloud Vertical Structure Optimization Algorithm Combining FY-4A and DSCOVR Satellite Data
by Zhuowen Zheng, Jie Yang, Taotao Lv, Yulu Yi, Zhiyong Lin, Jiaxin Dong and Siwei Li
Remote Sens. 2025, 17(14), 2484; https://doi.org/10.3390/rs17142484 - 17 Jul 2025
Viewed by 283
Abstract
Clouds are important for Earth’s energy budget and water cycles, and precisely characterizing their vertical structure is essential for understanding their impact. Although passive remote sensing offers broad coverage and high temporal resolution, sensor and algorithmic limitations impede the accurate depiction of cloud [...] Read more.
Clouds are important for Earth’s energy budget and water cycles, and precisely characterizing their vertical structure is essential for understanding their impact. Although passive remote sensing offers broad coverage and high temporal resolution, sensor and algorithmic limitations impede the accurate depiction of cloud vertical profiles. To improve estimates of their key structural parameters, e.g., cloud top height (CTH) and cloud vertical extent (CVE), we propose a multi-source collaborative optimization algorithm. The algorithm synergizes the wide-coverage FY-4A (FengYun-4A) and DSCOVR (Deep Space Climate Observatory) cloud products with high-precision CloudSat vertical profile data and establishes LightGBM-based CTH/CVE optimization models. The models effectively reduce systematic errors in the FY-4A and DSCOVR cloud products, lowering the CTH Mean Absolute Error (MAE) to 1.8 km for multi-layer clouds, an improvement of 4–8 km over the original. The CVE MAEs for single- and multi-layer clouds are ~2.5 km. Some bias remains in complex cases, e.g., multi-layer thin clouds at low altitudes, and error tracing analysis suggests this may be related to cloud layer number misclassification. The proposed algorithm facilitates daytime near-hourly cloud retrievals over China and neighboring regions. Full article
(This article belongs to the Section Atmospheric Remote Sensing)
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18 pages, 7358 KiB  
Article
On the Hybrid Algorithm for Retrieving Day and Night Cloud Base Height from Geostationary Satellite Observations
by Tingting Ye, Zhonghui Tan, Weihua Ai, Shuo Ma, Xianbin Zhao, Shensen Hu, Chao Liu and Jianping Guo
Remote Sens. 2025, 17(14), 2469; https://doi.org/10.3390/rs17142469 - 16 Jul 2025
Viewed by 213
Abstract
Most existing cloud base height (CBH) retrieval algorithms are only applicable for daytime satellite observations due to their dependence on visible observations. This study presents a novel algorithm to retrieve day and night CBH using infrared observations of the geostationary Advanced Himawari Imager [...] Read more.
Most existing cloud base height (CBH) retrieval algorithms are only applicable for daytime satellite observations due to their dependence on visible observations. This study presents a novel algorithm to retrieve day and night CBH using infrared observations of the geostationary Advanced Himawari Imager (AHI). The algorithm is featured by integrating deep learning techniques with a physical model. The algorithm first utilizes a convolutional neural network-based model to extract cloud top height (CTH) and cloud water path (CWP) from the AHI infrared observations. Then, a physical model is introduced to relate cloud geometric thickness (CGT) to CWP by constructing a look-up table of effective cloud water content (ECWC). Thus, the CBH can be obtained by subtracting CGT from CTH. The results demonstrate good agreement between our AHI CBH retrievals and the spaceborne active remote sensing measurements, with a mean bias of −0.14 ± 1.26 km for CloudSat-CALIPSO observations at daytime and −0.35 ± 1.84 km for EarthCARE measurements at nighttime. Additional validation against ground-based millimeter wave cloud radar (MMCR) measurements further confirms the effectiveness and reliability of the proposed algorithm across varying atmospheric conditions and temporal scales. Full article
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