Search Results (802)

The aim was to investigate the genetic effects of a SNP located in the precursor region of gga-miR-3528. (1) Single-nucleotide polymorphisms within precursor regions of microRNAs play crucial biological roles. (2) Utilizing a Gushi–Anka F₂ resource population (n = 860), we screened and validated miRNA SNPs. A SNP mutation in the miR-3528 precursor region was identified. Specific primers were designed to amplify the polymorphic fragment. Genotyping was performed for this individual SNP across the population, using the MassArray system. Association analyses were conducted between this SNP and chicken growth and body measurement traits, carcass traits, meat quality traits, and serum enzyme activities. (3) The rs14098602 (+12 bp A > G) was identified within the precursor region of gga-miR-3528. Significant associations (p < 0.05) were observed between this SNP and chicken growth traits (body weight at the age of 0 day, body weight at the age of 2 weeks, and body weight at the age of 4 weeks), carcass traits (evisceration weight), meat quality traits (subcutaneous fat rate and pectoral muscle density), and serum enzyme activities (total protein, albumin, globulin, cholinesterase, and lactate dehydrogenase). (4) These findings suggest that the polymorphism at rs14098602 may influence chicken growth, meat quality, and serum biochemical indices, through specific mechanisms. The gga-miR-3528 gene likely plays an important role in chicken development. Therefore, this SNP can serve as a molecular marker for genetic breeding and auxiliary selection of growth-related traits, facilitating the rapid establishment of elite chicken populations with superior genetic resources. Full article

Rickettsia, Anaplasma, and Ehrlichia species are emerging tick-borne pathogens that cause zoonotic diseases, including rickettsiosis, anaplasmosis, and ehrlichiosis in both human and animal populations. This study aimed to investigate the prevalence of these pathogens in cattle-associated ticks from Ganzhou City, Jiangxi Province, China. Through molecular characterization using multilocus sequence analysis (16S rRNA, gltA, groEL, and ompA genes), we analyzed 392 Rhipicephalus microplus ticks collected from March to September in 2022. The PCR results showed that eight Rickettsiales bacteria were detected, including two species of Rickettsia (51/392, 13.0%), four species of Anaplasma (52/392, 13.3%), and two species of Ehrlichia (70/392, 17.9%). Notably, the circulation of multiple pathogen species within R. microplus populations demonstrates significant microbial diversity in this region. Further consideration and investigation should be given to the possible occurrence of rickettsiosis, ehrlichiosis, and anaplasmosis in humans and domestic animals. Our study provides critical baseline data for developing targeted surveillance strategies and informing public health interventions against tick-borne diseases in southeastern China. Full article

Background: During food processing and storage, traditional protein-based delivery systems encounter significant challenges in maintaining the structural and functional integrity of bioactive compounds, primarily due to their temporal instability. Methods: In this study, a nanocomposite hydrogel was prepared through the co-assembly of a self-assembling peptide, 9-Fluorenylmethoxycarbonyl-phenylalanine-arginine-glycine-aspartic acid-phenylalanine (Fmoc-FRGDF), and hyaluronic acid (HA). The stability of this hydrogel as a quercetin (Que) delivery carrier was systematically investigated. Furthermore, the impact of Que co-assembly on the microstructural evolution and physicochemical properties of the hydrogel was characterized. Concurrently, the encapsulation efficiency (EE%) and controlled release kinetics of Que were quantitatively evaluated. Results: The findings indicated that HA significantly reduced the storage modulus (G′) from 256.5 Pa for Fmoc-FRGDF to 21.1 Pa with the addition of 0.1 mg/mL HA. Despite this reduction, HA effectively slowed degradation rates; specifically, residue rates of 5.5% were observed for Fmoc-FRGDF alone compared to 14.1% with 0.5 mg/mL HA present. Notably, Que enhanced G′ within the ternary complex, increasing it from 256.5 Pa in Fmoc-FRGDF to an impressive 7527.0 Pa in the Que/HA/Fmoc-FRGDF hydrogel containing 0.1 mg/mL HA. The interactions among Que, HA, and Fmoc-FRGDF involved hydrogen bonding, electrostatic forces, and hydrophobic interactions; furthermore, the co-assembly process strengthened the β-sheet structure while significantly promoting supramolecular ordering. Interestingly, the release profile of Que adhered to the Korsmeyer–Peppas pharmacokinetic equations. Conclusions: Overall, this study examines the impact of polyphenol on the rheological properties, microstructural features, secondary structure conformation, and supramolecular ordering within peptide–polysaccharide–polyphenol ternary complexes, and the Fmoc-FRGDF/HA hydrogel system demonstrates a superior performance as a delivery vehicle for maintaining quercetin’s bioactivity, thereby establishing a multifunctional platform for bioactive agent encapsulation and controlled release. Full article

The context of global climate change, water stress has a significant impact on the ecological function and landscape value of urban greening shrubs. In this study, three typical greening shrubs (Euonymus japonicus, Ligustrum × vicaryi, and Berberis thunbergii var. atropurpurea) in North China were subjected to a two-year field-controlled experiment (2022–2023) with four water treatments: full irrigation, deficit irrigation, natural rainfall, and extreme drought. The key findings are as follows: (1) Extreme drought reduced the color indices substantially—the G_CC of E. japonicus decreased by 40% (2023); the R_CC of B. thunbergii var. atropurpurea declined by 35% (2022); and the color indices of L. × vicaryi remained stable (variation < 15%). (2) Early-season soil water content (SWC) strongly correlated with the color index of E. japonicus (r² = 0.42, p < 0.05) but weakly with B. thunbergii (r² = 0.28), suggesting species-specific drought-tolerance mechanisms like reduced leaf area. (3) Deficit irrigation (SWC ≈ 40%) maintained color indices between fully irrigated and drought-stressed levels. Notably, B. thunbergii retained high redness (R_CC > 0.8) at an SWC ≈ 40%; E. japonicus required an SWC > 60% to preserve greenness (G_CC). The research results provide a scientific basis for urban greening plant screening and water-saving irrigation strategies, and expand the application scenarios of color coordinates in plant physiological and ecological research. Full article

This study evaluated the wind speed simulation performance of the Weather Research and Forecasting (WRF) model at three resolutions in Hebei Province based on wind speed data from 2022. The results show that the simulation effectiveness of the WRF model for wind speeds at different heights varies significantly under different seasons and topographic conditions. In general, the model simulates the wind speed at the high level most accurately, followed by the mid level, and the simulation of low level wind speed shows the largest bias. Increasing the model resolution significantly improves the simulation of low-level wind speed, and the 5 km resolution performs best at most stations; while for the mid-level and high-level wind speeds, increasing the resolution does not significantly improve the simulation effect, and the high-resolution simulation has a greater bias at some stations. In terms of topographic features, wind speeds are generally better simulated in mountainous areas than in the plains during spring, summer, and autumn, while the opposite is true in winter. These findings provide scientific reference for WRF model optimal resolution selection and wind resource assessment. Full article

Background/Objectives: Alveolar echinococcosis (AE), caused by Echinococcus multilocularis larvae, poses a significant global health concern. Primarily affecting regions in the northern hemisphere, such as northwest China, which are vital for animal husbandry, it often results in severe hepatic impairment in the host. However, there remains a dearth of knowledge concerning changes in gene expression profiles during the progression of AE. In this study, we employed transcriptome sequencing (RNA sequencing, RNA-Seq) to detect alterations in gene expression profiles in the liver tissues of mice with AE. Our aims were to understand the transcriptome differences in the liver during E. multilocularis infection and to explore the molecular mechanisms underlying the early progression of this disease. Methods: We established a mouse model of AE by intraperitoneally injecting protoscoleces of E. multilocularis. All the inoculated mice were randomly divided into four groups. Liver tissues were collected at 6, 12, 19, and 25 weeks after inoculation. Paired non-infected mouse-derived liver tissues were used as controls, and transcriptome sequencing was carried out. Results: A total of 629 differentially expressed genes (DEGs) were identified. Among them, 370 genes were upregulated and 259 genes were downregulated. Moreover, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated that these DEGs were significantly associated with immune system modulation, the cell cycle, and the fibrosis process during the pathological changes. Additionally, weighted gene co-expression network analysis (WGCNA) identified several genes, including CCNA2, BIRC5, KIF2C, OTC, TLR2, and NCKAP1L. These hub genes involved in immunoinflammatory processes may be related to E. multilocularis larvae infection. Conclusions: The findings of this research provide a theoretical foundation for a more in-depth understanding of the molecular mechanisms of AE. They offer valuable insights into the molecular mechanisms and potential key factors involved in the pathogenesis of this disease. Full article

Aurora kinase B (AURKB), a serine/threonine protein kinase, is essential for accurate chromosome segregation and cytokinesis during mitosis. Dysregulation of AURKB, often characterized by its overexpression, has been implicated in various malignancies, including breast cancer. However, the mechanisms governing its dysregulation remain incompletely understood. Here, we identify a pivotal role for the MOF/MSL complex—which includes the histone acetyltransferase MOF (KAT8)—in modulating AURKB stability through acetylation at lysine 215 (K215). This post-translational modification inhibits AURKB ubiquitination, thereby stabilizing its protein levels. MOF/MSL-mediated AURKB stabilization promotes the proper assembly of the chromosomal passenger complex (CPC), ensuring mitotic fidelity. Notably, inhibition of MOF reduces AURKB K215 acetylation, leading to decreased AURKB expression and activity. Consequently, this downregulation suppresses expression of the downstream oncogene c-MYC, ultimately attenuating the malignant proliferation of breast cancer cells. Collectively, our findings reveal a novel mechanism by which lysine acetylation regulates AURKB stability, highlight the significance of the MOF-AURKB-c-MYC axis in breast cancer progression, and suggest potential therapeutic strategies targeting this pathway in clinical settings. Full article

Nicotine-induced oxidative stress contributes significantly to vascular endothelial dysfunction. While negative air ions (NAIs) demonstrate potential blood-pressure-regulating and antioxidant properties, their mechanistic role remains unclear. This study examined the effects of NAIs against nicotine-induced oxidative damage and vascular endothelial injury in spontaneously hypertensive rats (SHRs). Western blotting was used to detect the expression levels of the α7nAChR/MAPK/AP1 pathway. Transcriptomic sequencing was performed to identify the differentially expressed genes after treatment with nicotine or NAIs. Furthermore, reactive oxygen species (ROS), endothelin-1 (ET-1), and [Ca²⁺]_i levels were detected in human aortic endothelial cells (HAECs) treated with nicotine, and the relationship between transcription factor activator protein 1 (AP1) and the target genes was further elucidated through ChIP–qPCR. Nicotine exposure in SHRs elevated blood pressure and induced oxidative damage through α7nAChR/MAPK/AP1 pathway activation, causing endothelial structural disruption. These effects manifested as decreased NO/eNOS and increased ET-1/ET_ab expression, while these changes were reversed by NAIs. In HAECs, nicotine impaired proliferation while increasing oxidative stress and [Ca²⁺]_i levels. This endothelial damage was markedly attenuated by either NAIs or fibronectin 1 (Fn1)/secreted phosphoprotein 1 (Spp1) knockdown. Mechanistically, we identified AP1 as the transcriptional regulator of FN1 and SPP1. NAIs attenuate nicotine-induced endothelial dysfunction in hypertension by inhibiting AP1-mediated FN1 and SPP1 activation, providing novel insights for smoking-associated cardiovascular risk. Full article

Vermipsyllid parasitize the body surface of sheep, feeding on blood and transmitting diseases, causing severe economic losses to the livestock industry. An outbreak of sheep Vermipsyllid fleas in the mountain pastures of Xinjiang’s Altai region showed that several commonly used commercial anthelmintic drugs had poor therapeutic effects on the prevalent flea species. This study first conducted species identification of Vermipsyllid through morphological analysis of 200 female and 40 male specimens collected from the Altai region, followed by molecular biological identification of 6 randomly selected fleas (3 females and 3 males). Finally, pharmacodynamic experiments were performed to screen for highly effective anthelmintic drugs. Ninety Altai multiparous ewes infected with Vermipsyllid were divided into six groups (fifteen sheep per group): an untreated control group (Ctr), avermectin injection group (Group I), ivermectin injection group (Group II), moxidectin pour-on group (Group III), cypermethrin pour-on group (Group IV), and dichlorvos pour-on group (Group V), with a 14-day trial period. On Day 0, each group received a single treatment according to drug instructions and specified dosages. The number of fleas, flea population reduction rates, and cure rates were measured on Day 0, Day 3, Day 7, and Day 14 to screen for effective anthelmintic drugs. Results showed that unfed female and male fleas were grayish-brown. Engorged females reached 14.15 mm in size, appearing pale white or yellow, with their sterna maintaining the original size and shape despite abdominal distension. Female fleas had 20–21 segments on the labium, while males had 16–19 segments. The hind tibiae featured four notches, and each side of the tergite had 13 ± 1 cup-like indentations. Molecular biological identification indicated that the identified fleas belonged to D. ioffi (Vermipsyllidae, Dorcadia Ioff), showing 99.13% sequence similarity with D. ioffi from Xinjiang, China. In pharmacodynamic experiments, the number of D. ioffi in Groups IV and V decreased to zero on both Day 7 and Day 14, which was extremely significantly lower than other groups (p < 0.01). The flea population reduction rates in Groups IV and V reached 100% on both days. By Day 14, the cure rates of Groups I, II, and III were 0%, while those of Groups IV and V were 100%. Avermectin injection, ivermectin injection, and moxidectin pour-on showed poor anthelmintic effects, whereas cypermethrin and dichlorvos pour-on exhibited high anthelmintic activity against this flea species. Full article

Background: This study aimed to develop a predictive model to assess risk factors and prognoses in pediatric patients with dilated cardiomyopathy (DCM). Methods: A total of 233 pediatric patients with DCM who were hospitalized between January 2019 and June 2024 were enrolled. The children were followed up and categorized into two groups: the death/heart transplantation (D/HT) group and the non-D/HT group. Univariate and multivariate analyses identified risk factors. A nomogram model and a scoring system were developed. The performance of these models was evaluated using the H-L test, ROC analysis, and internal validation. Results: The results demonstrated that the age of onset, cardiac functional classification III–IV, moderate-to-severe mitral regurgitation, low voltage in limb leads on an ECG, and the need for vasoactive drugs are independent predictors of D/HT risk in children with DCM. A nomogram model was developed, achieving an AUC of 0.804 (95% CI: 0.734–0.874), a sensitivity of 80.3%, and a specificity of 66.7%. A scoring system was established: 1 point for age of onset, 10 points for cardiac functional classification III–IV, 2.5 points for moderate-to-severe mitral regurgitation, 4 points for low voltage in limb leads on an ECG, 3 points for the need for vasoactive drugs, or 0 points if none of these criteria were met. When the cumulative score was ≥ 13.25, the sensitivity and specificity increased to 68.9% and 73.9%, respectively. Conclusions: We developed both a nomogram and a scoring system model, which are capable of rapidly and accurately predicting the risk of D/HT in children with DCM. Full article

To promote the optimization of the anti-friction and anti-wear behavior of lightweight TiAl alloys, γ-TiAl-10 wt.% Ag self-lubricating composites were fabricated, and their mechanical and tribological properties were tested. The results showed that the silver in TiAl-10 wt.% Ag slightly reduced its mechanical properties compared with those of pure TiAl alloys. A silver-enriched lubrication film formed on a wear scar, which was helpful in improving the friction and wear behavior. It was found that a large amount of silver gathered at a wear scar, gradually spread out under the action of the sliding friction force, and then increased the silver distribution areas on the wear scar, leading to the good formation of a silver-rich film. Furthermore, an identification model was established to calculate the specific area η of the silver film. A quantitative relationship indicated that an increase in the Ag distribution area improved the tribological behavior of γ-TiAl-10 wt.% Ag. When the specific area η of a silver-rich film was maintained at 44–51%, the small friction coefficient (almost 0.28) and wear rate (about 2.25 × 10⁻⁴ mm³·N⁻¹·m⁻¹) were well stabilized. This provides a new research method to improve the tribological performance of TiAl-Ag samples. Full article

Background/Objectives: Panax notoginseng stems and leaves (PNSLs) have shown limited adoption as a forage component in commercial livestock operations due to low utilization rates. Methods: This study was designed to add 10% and 20% PNSLs in the diet of Duzang pigs. Hepatic tissues were collected to investigate the expression levels of Toll-like receptor (TLR), MyD88, TRIF genes, and downstream cytokines within the TLR signaling pathway. Results: 10% and 20% PNSLs in the diet significantly up-regulated mRNA expression levels of TLR3, TLR6, TLR7, TLR8, TRIF, IL-10, IFN-α and IFN-β, while they down-regulated TLR5, TLR9, TNF-α and IL-1β in Duzang pigs. No significant effects were observed on the expression of TLR2 and IL-6. Conclusions: Different amounts of PNSLs modulated the TLR signaling pathway mRNA expression levels in the hepatic tissues of Duzang pigs. Full article

Vegetable oil is regarded as a medium that can replace kerosene in electrical discharge machining (EDM) hole processing due to its renewability and environmental friendliness. Meanwhile, numerical simulation serves as an effective means to study the behavior of the gap flow field during EDM processing. Based on this, this study explored the influence of hole size and different vegetable oil dielectrics (sunflower seed oil, canola oil, and soybean oil) on the movement of electro-corrosion residues in the processing gap. The simulation results demonstrate that the viscosity of the oil affects the escape rate of the particles. In holes of 1 mm and 4 mm of size, the escape rate of canola oil at any time period is superior to that of sunflower seed oil and soybean oil. In a 1 mm hole, its average escape rate reached 19.683%, which was 0.24% and 0.19% higher than that of sunflower seed oil and soybean oil, respectively. Subsequently, experiments were conducted in combination with the simulation results to explore the influence of current, pulse width, and pulse interval on hole processing. This further confirmed the application potential of vegetable oil in electrical discharge micro-hole processing and provided theoretical support and experimental basis for optimizing the green manufacturing process. Full article

In order to determine the failure reason for the non-working area of a cracked A-pillar hot-stamping die insert, various instruments were used to detect the properties and microstructures of the cracks and matrix. The results show that the cracks are located in the area where the oxidative corrosion is more serious, and the cracks do not appear in the pitting area, verifying that crack initiation is related to the stress concentration on the upper half of the inner wall of the cooling channel. Meanwhile, pores and cracks exist in the grain boundary and crystal, making the impact energy of the die steel poor. Therefore, crack initiation and propagation easily occur along the brittle oxide layer. In summary, the die insert is damaged by stress-induced corrosion. In engineering applications of hot-stamping dies, we should pay more attention to the cracking of the cooling channel caused by stress and corrosion. Full article

This study analyzes a lacquered ear cup excavated from the Luobowan tomb complex in Guigang, Guangxi, attributed to the Nanyue Kingdom of the early Han dynasty. A range of analytical techniques, including optical microscopy (OM), scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDS), pyrolysis–gas chromatography–mass spectrometry (Py-GC-MS), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD), were employed to investigate the structural layers, material composition, and preservation state of the artifact. The lacquerware consists of four traditional layers: a wooden core, fabric reinforcement, lacquer ground, and lacquer film, reflecting Central Plains lacquerware techniques. The wooden core was identified as Phoebe sp., and the fabric layer is likely hemp, though fiber degradation limited exact identification. The lacquer ground layer contains natural lacquer mixed with SiO₂ from brick or tile powder. The lacquer film is a blend of Chinese and Vietnamese lacquer, with no synthetic additives or plant oils detected. The red lacquer layer contains cinnabar (HgS) as a pigment, while the black lacquer uses carbon black. Differences in moisture content between the red and black lacquer films are attributed to variations in surface porosity and pigment characteristics. This research provides valuable insights into Nanyue lacquer technology and preservation challenges. Full article