Natural Product Separation and Characterization of Bioactive Plant Compounds

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Analysis of Natural Products and Pharmaceuticals".

Deadline for manuscript submissions: 30 May 2026 | Viewed by 747

Special Issue Editor


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Guest Editor
Laboratory of Phytochemistry and Biomolecules, Department of Chemistry, State University of Londrina (UEL), Londrina 86051–990, PR, Brazil
Interests: pyrrolizidine alkaloids; phenolic compounds; extract

Special Issue Information

Dear Colleagues,

Natural products continue to be an inexhaustible source of structurally diverse and biologically active compounds. Advances in chromatographic and spectrometric techniques have significantly expanded our ability to separate, purify, and characterize bioactive molecules from plants and other natural sources. These methodological innovations play a pivotal role not only in natural product chemistry but also in pharmaceutical, food, cosmetic, and biotechnology research.

This Special Issue of Separations, entitled “Natural Product Separation and Characterization of Bioactive Plant Compounds,” aims to highlight recent developments in separation science applied to natural bioactive compounds. We welcome the submission of original research papers, communications, and comprehensive reviews addressing, but not limited to, the following topics:

  • Innovative chromatographic and hyphenated techniques for natural product isolation;
  • Advances in sample preparation and purification of bioactive metabolites;
  • Analytical strategies for structural elucidation and quality control;
  • Applications of separation science in pharmacology, nutraceuticals, and related fields.

We invite you and your collaborators to contribute to this Special Issue. Your expertise will help provide an up-to-date overview of how separation science is currently driving progress in natural product research.

We look forward to receiving your valuable contributions.

Dr. Maria Luiza Zeraik
Guest Editor

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Keywords

  • natural products
  • bioactive compounds
  • plant metabolites
  • chromatographic separation
  • hyphenated techniques
  • sample preparation
  • metabolite profiling
  • structural characterization
  • phytochemistry
  • analytical chemistry

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Published Papers (2 papers)

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Research

16 pages, 1533 KB  
Article
Horseradish (Armoracia rusticana L.) Processing By-Products as Potential Functional Ingredients in Food Production: A Detailed Insight into Phytochemical Composition and Antioxidant Properties
by Jovana M. Marković, Ana S. Salević, Danijel D. Milinčić, Uroš M. Gašić, Verica B. Đorđević, Biljana B. Rabrenović, Mirjana B. Pešić, Steva M. Lević, Dragana M. Mihajlović and Viktor A. Nedović
Separations 2025, 12(12), 330; https://doi.org/10.3390/separations12120330 - 29 Nov 2025
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Abstract
Horseradish (Armoracia rusticana L.) root (HRP) and leaf (HLP) pomaces, by-products of juice production by cold-pressing, were analyzed as a novel potential source of natural antioxidants. Chromatography analysis (UHPLC Q-ToF MS) of the bioactive compounds of pomaces was performed along with spectrophotometric [...] Read more.
Horseradish (Armoracia rusticana L.) root (HRP) and leaf (HLP) pomaces, by-products of juice production by cold-pressing, were analyzed as a novel potential source of natural antioxidants. Chromatography analysis (UHPLC Q-ToF MS) of the bioactive compounds of pomaces was performed along with spectrophotometric determination of total phenolic content (TPC), total flavonoid content (TFC), total phenolic acid (hydroxycinnamic) content (TPAC), and antioxidant capacity (via 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic-acid) (ABTS•+) radicals’ scavenging activity and ferric reducing antioxidant power (FRAP)). The concentrations of TPC, TFC, and TPAC differed among the pomaces, significantly favoring HLP. However, both horseradish pomaces (HRP and HLP) contained a considerable amount of various phenolics, with kaempferol and its glucosides dominating. In addition, they exhibit pronounced antioxidant activity, which is confirmed by all three methods used (DPPH, ABTS, and FRAP). These results highlight the potential of valorizing horseradish processing waste as a natural, reliable source of health-promoting bioactive compounds and functional ingredients in food products, thereby fortifying food, preventing oxidation, and prolonging shelf-life. In addition, this study supports endeavors to reduce food waste by providing new insights into the valorization of horseradish pomace, thus contributing to sustainable development and environmental protection. Full article
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14 pages, 1584 KB  
Article
UHPLC-QTOF-MS/MS Metabolomic Profiling Reveals Environmental, Morphological, and Taxonomic Drivers of Chemical Diversity in Neotropical Banisteriopsis and Stigmaphyllon
by Jaqueline Munise Guimarães da Silva, Grazielle Jesus dos Santos, Rafael Felipe de Almeida and Maria Luiza Zeraik
Separations 2025, 12(11), 323; https://doi.org/10.3390/separations12110323 - 20 Nov 2025
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Abstract
Banisteriopsis and Stigmaphyllon are among the most species-rich liana genera in the Neotropical Malpighiaceae family, known for their diverse chemical and biological activities. However, metabolomic information correlating these genera with their biome of occurrence, humidity levels, and plant habit (liana vs. shrub) remains [...] Read more.
Banisteriopsis and Stigmaphyllon are among the most species-rich liana genera in the Neotropical Malpighiaceae family, known for their diverse chemical and biological activities. However, metabolomic information correlating these genera with their biome of occurrence, humidity levels, and plant habit (liana vs. shrub) remains limited. This study explored in detail the metabolomic profiles of 15 species (29 specimens) of Banisteriopsis and 26 species (35 specimens) of Stigmaphyllon using Partial Least Squares Discriminant Analysis (PLS-DA). Leaf extracts obtained with ethanol/water (4:1, v/v) were analyzed by Ultra Performance Liquid Chromatography coupled to Quadrupole Time-of-Flight Mass Spectrometry (UHPLC-QTOF-MS/MS). Comparative analyses were performed at the genus level and across biome, humidity, and plant habit variables. The UHPLC-MS/MS profiling enabled the annotation of compounds, including previously unreported metabolites identified in B. quadriglandula and S. saxicola. Shared metabolites between the two genera were also characterized. PLS-DA revealed discriminant metabolites associated with the different comparative parameters, notably glucose (plant habit), coumaroyl hexoside, myricetin-3-galactoside, quercetin (genus), and quercetin-3-O-robinobioside (environment). With 95% confidence, our results demonstrate that environmental and morphological factors significantly influence metabolite biosynthesis in Banisteriopsis and Stigmaphyllon, providing valuable insights for future phytochemical and ecological research in the Neotropics. Full article
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