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Live Cell-Based Sensors with Receptors

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: closed (15 May 2014) | Viewed by 5311

Special Issue Editor

Research Center for Functional Materials, National Institute for Materials Science (NIMS), Tsukuba, Ibaraki, Japan
Interests: study of interaction between nanomaterials and cells
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

A biosensor is an analytical device which combines a biological component with a physiochemical detection system to detect, transmit and record the desired information. A live cell-based sensor, as one of the important biosensor members, is well accepted and used for investigating various signaling cascades caused by cytotoxic effects of reagents. A basic assembly of a live cell-based sensor consists of a receptor and a transducer. When the stimuli get in touch with the cell, they pass through the cell plasma membrane with the help of receptors. The receptors are a very important part of live cell-based sensors. The Special Issue of the journal Sensors will cover these different types of live cell-based sensors with receptors, and consider applications for these different fields.

Prof. Dr. Akiyoshi Taniguchi
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


Keywords

  • receptor
  • microbial cells
  • mammalian cells
  • biotechnology
  • biosensor
  • cell culture
  • cytotoxicity

Published Papers (1 paper)

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Research

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Article
Quantitative Analysis of Staphylococcus Enterotoxin A by Differential Expression of IFN-γ in Splenocyte and CD4+ T-Cells
by Reuven Rasooly and Bradley J. Hernlem
Sensors 2014, 14(5), 8869-8876; https://doi.org/10.3390/s140508869 - 20 May 2014
Cited by 9 | Viewed by 4929
Abstract
Staphylococcus aureus is an important bacterial pathogen that produces a range of Staphylococcal Enterotoxins (SEs) which cause gastroenteritis and superantigen activation of T cells, the mechanism of which is not well understood. The ability to rapidly detect and quantify SEs is very important [...] Read more.
Staphylococcus aureus is an important bacterial pathogen that produces a range of Staphylococcal Enterotoxins (SEs) which cause gastroenteritis and superantigen activation of T cells, the mechanism of which is not well understood. The ability to rapidly detect and quantify SEs is very important in order to learn the causes of staphylococcal outbreaks and to stop similar outbreaks in the future. Enzyme-linked immunosorbent assays (ELISAs) have been developed for detection of several SEs. However, these immunological methods cannot distinguish between active and inactive toxin. It is known that interferon-gamma (IFN-γ) expressed in response to stimulation by SEs contributes significantly to the pathogenesis of S. aureus infection. Nonetheless, the cellular source of IFN-γ is still unclear and the contributions of the specific splenocyte types. In our effort to understand the immunologic response to Staphylococcal Enterotoxin A (SEA) exposure, we studied IFN-γ production in mouse splenocytes. We demonstrated that short term ex vivo exposure of splenocytes or primary naïve CD4+ T-cells to biologically active SEA induces differential expression of IFN-γ mRNA in a time and dose dependent manner and the expression levels reflect the levels of IFN-γ secreted protein. Positive isolated CD4+ T-cells accounted for only 10% of IFN-γ production. We also demonstrate that expression of IFN-γ can be used for rapid quantitative analysis of active SEA with a detection limit of 1 ng/mL. Full article
(This article belongs to the Special Issue Live Cell-Based Sensors with Receptors)
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