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Special Issue "Surface Plasmon Resonance (SPR)-Based Sensors and Their Biological Applications"

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: 30 August 2019

Special Issue Editors

Guest Editor
Dr. Victoria Shpacovitch

Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., Bunsen-Kirchhoff-Straße 11, 44139 Dortmund, Germany
E-Mail
Interests: SPR; bioconjugation; sensor surface functionalization; extracellular vesicles and their characterization; viruses and virus-like particles
Guest Editor
Dr. Roland Hergenröder

Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., Bunsen-Kirchhoff-Straße 11, 44139 Dortmund, Germany
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Interests: study of surfaces; biological interfaces; diagnostic tools

Special Issue Information

Dear Colleagues,

Surface plasmon resonance (SPR)-based sensors are the subject of a growing scientific and practical interest mainly due to their high sensitivity and versatility, as well as their ability to perform the label-free detection of bio-particles or bio-molecules. These traits make SPR-based sensors ideal platforms for the development of diverse chemical and biological assays. Nowadays, SPR-based sensors are widely used in different research and practical fields, such as pharmacology, biomedical science, environmental monitoring, food science, and others. In future, the integration of SPR-based sensing platforms in small research groups or in point of care clinical units will require the development of miniaturized, user-friendly, and low-cost instruments. Thus, this Special Issue is planned to highlight not only the newest scientific developments in biomedical applications of SPR-based sensors, but also to indicate novel trends in the development and engineering of SPR sensing platforms.

Dr. Victoria Shpacovitch
Dr. Roland Hergenröder
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • SPR-based sensors
  • SPR microscopy
  • novel applications of SPR sensors
  • optical fiber sensors
  • localized SPR sensors
  • biomedical sensors
  • label-free sensors
  • real-time monitoring
  • analytical performance

Published Papers (3 papers)

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Research

Open AccessArticle
Development of SPR Imaging-Impedance Sensor for Multi-Parametric Living Cell Analysis
Sensors 2019, 19(9), 2067; https://doi.org/10.3390/s19092067
Received: 12 March 2019 / Revised: 22 April 2019 / Accepted: 28 April 2019 / Published: 3 May 2019
PDF Full-text (3205 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Label-free evaluation and monitoring of living cell conditions or functions by means of chemical and/or physical sensors in a real-time manner are increasingly desired in the field of basic research of cells and clinical diagnosis. In order to perform multi-parametric analysis of living [...] Read more.
Label-free evaluation and monitoring of living cell conditions or functions by means of chemical and/or physical sensors in a real-time manner are increasingly desired in the field of basic research of cells and clinical diagnosis. In order to perform multi-parametric analysis of living cells on a chip, we here developed a surface plasmon resonance (SPR) imaging (SPRI)-impedance sensor that can detect both refractive index (RI) and impedance changes on a sensor chip with comb-shaped electrodes. We then investigated the potential of the sensor for label-free and real-time analysis of living cell reactions in response to stimuli. We cultured rat basophilic leukemia (RBL)-2H3 cells on the sensor chip, which was a glass slide coated with comb-shaped electrodes, and detected activation of RBL-2H3 cells, such as degranulation and morphological changes, in response to a dinitro-phenol-conjugated human serum albumin (DNP-HSA) antigen. Moreover, impedance analysis revealed that the changes of impedance derived from RBL-2H3 cell activation appeared in the range of 1 kHz–1 MHz. Furthermore, we monitored living cell-derived RI and impedance changes simultaneously on a sensor chip using the SPRI-impedance sensor. Thus, we developed a new technique to monitor both impedance and RI derived from living cells by using a comb-shaped electrode sensor chip. This technique may enable us to clarify complex living cell functions which affect the RI and impedance and apply this to medical applications, such as accurate clinical diagnosis of type I allergy. Full article
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Open AccessArticle
Sensitivity and Stability Enhancement of Surface Plasmon Resonance Biosensors based on a Large-Area Ag/MoS2 Substrate
Sensors 2019, 19(8), 1894; https://doi.org/10.3390/s19081894
Received: 8 March 2019 / Revised: 10 April 2019 / Accepted: 16 April 2019 / Published: 21 April 2019
Cited by 1 | PDF Full-text (3275 KB) | HTML Full-text | XML Full-text
Abstract
Surface plasmon resonance (SPR) sensors based on a silver film suffer from signal degradation due to silver oxidation in aqueous sensing environments. To overcome this limitation, we fabricated the planar plasmonic substrate employing an atomic MoS2 layer on a silver surface. Successful [...] Read more.
Surface plasmon resonance (SPR) sensors based on a silver film suffer from signal degradation due to silver oxidation in aqueous sensing environments. To overcome this limitation, we fabricated the planar plasmonic substrate employing an atomic MoS2 layer on a silver surface. Successful production of a large-area MoS2 monolayer blocks the penetration of oxygen and water molecules. In addition, we theoretically and experimentally found that MoS2 layer on the silver film can improve the SPR sensitivity and stability significantly. In this study, the proposed SPR substrate has the potential to provide highly enhanced sensor platforms for surface-limited molecular detections. Full article
Figures

Figure 1

Open AccessArticle
Early Detection of the Fungal Banana Black Sigatoka Pathogen Pseudocercospora fijiensis by an SPR Immunosensor Method
Sensors 2019, 19(3), 465; https://doi.org/10.3390/s19030465
Received: 20 November 2018 / Revised: 17 January 2019 / Accepted: 20 January 2019 / Published: 23 January 2019
PDF Full-text (2380 KB) | HTML Full-text | XML Full-text
Abstract
Black Sigatoka is a disease that occurs in banana plantations worldwide. This disease is caused by the hemibiotrophic fungus Pseudocercospora fijiensis, whose infection results in a significant reduction in both product quality and yield. Therefore, detection and identification in the early stages [...] Read more.
Black Sigatoka is a disease that occurs in banana plantations worldwide. This disease is caused by the hemibiotrophic fungus Pseudocercospora fijiensis, whose infection results in a significant reduction in both product quality and yield. Therefore, detection and identification in the early stages of this pathogen in plants could help minimize losses, as well as prevent the spread of the disease to neighboring cultures. To achieve this, a highly sensitive SPR immunosensor was developed to detect P. fijiensis in real samples of leaf extracts in early stages of the disease. A polyclonal antibody (anti-HF1), produced against HF1 (cell wall protein of P. fijiensis) was covalently immobilized on a gold-coated chip via a mixed self-assembled monolayer (SAM) of alkanethiols using the EDC/NHS method. The analytical parameters of the biosensor were established, obtaining a limit of detection of 11.7 µg mL−1, a sensitivity of 0.0021 units of reflectance per ng mL−1 and a linear response range for the antigen from 39.1 to 122 µg mL−1. No matrix effects were observed during the measurements of real leaf banana extracts by the immunosensor. To the best of our knowledge, this is the first research into the development of an SPR biosensor for the detection of P. fijiensis, which demonstrates its potential as an alternative analytical tool for in-field monitoring of black Sigatoka disease. Full article
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