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Point-of-Care Biosensors: Design and Applications

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: 15 June 2025 | Viewed by 1010

Special Issue Editors


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Guest Editor
Department of Biosciences, Rice University, Houston, TX, USA
Interests: Developing electrochemical methods and engineering proteins for point-of-care diagnostics and biosynthesis.

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Guest Editor
Department of Biosciences, Rice University, Houston, TX, USA
Interests: Engineering biological molecules and microbes to address global challenges in energy and the environment.

Special Issue Information

Dear Colleagues,

Point-of-care biosensors represent a multidisciplinary field at the forefront of personalized medicine, merging fundamental and applied research with profound implications across science, technology, and industry. These biosensors, epitomized by the widely used glucometer, detect analytes directly at or near the location where medical care is provided, showcasing significant advances and opportunities within healthcare. These tools have substantially benefited medicine by providing patients with inexpensive and accurate diagnoses without burdensome hospital visits. Today, point-of-care biosensors have expanded beyond blood glucose monitoring to include detecting infectious diseases and monitoring cardiovascular health. Their development spans disciplines such as electrochemistry, nanotechnology, materials science, synthetic biology, transistors, and more.

In this Special Issue, we aim to cover a wide range of research on point-of-care biosensors. We address the design of sensing machinery at the molecular level, the development of various detection methods, and the integration of these crucial components into miniature or wearable devices. By exploring these key aspects, our goal is to offer a relatively comprehensive picture of the latest advancements in point-of-care biosensors and tackle the transformative challenge of bringing these innovative devices from the laboratory to the market.

Dr. Rong Cai
Dr. Caroline Ajo-Franklin
Guest Editors

Manuscript Submission Information

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Keywords

  • point-of-care
  • personalized medicine
  • biosensor
  • glucometer
  • electrochemistry
  • protein engineering
  • synthetic biology
  • nanoenzyme
  • microneedle
  • organic electrochemical transistor
  • filed effect transistor
  • electronic skin
  • wearable device
  • colorimetric assay
  • paper-based sensor

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Published Papers (1 paper)

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Research

15 pages, 8175 KiB  
Article
Aptamer Paper-Based Fluorescent Sensor for Determination of SARS-CoV-2 Spike Protein
by Jincai Yang, Zunquan Zhao, Tianyi Ma and Jialei Bai
Sensors 2025, 25(6), 1637; https://doi.org/10.3390/s25061637 - 7 Mar 2025
Viewed by 623
Abstract
Point-of-care (POC) antigen detection plays a crucial role in curbing the spread of viruses. Paper-based fluorescence aptasensors are expected to offer a low-cost tool to meet the needs of decentralized POC diagnosis. Herein, we report on a fluorescent paper-based sensing system for detecting [...] Read more.
Point-of-care (POC) antigen detection plays a crucial role in curbing the spread of viruses. Paper-based fluorescence aptasensors are expected to offer a low-cost tool to meet the needs of decentralized POC diagnosis. Herein, we report on a fluorescent paper-based sensing system for detecting the SARS-CoV-2 spike protein. The sensing system was constructed by loading multi-layer Nb2C MXene nano-quenchers and carbon-dot-labeled aptamer (G-CDs@Apt) probes onto a mixed cellulose ester (MCE) paper substrate. On the Nb2C MXene/G-CDs@Apt sensing paper, abundant G-CDs@Apt probes were attached to the multilayer MXene nano-quenchers and kept in a fluorescence-off state, while recognition of the target detached the G-CDs@Apt probes formed the nano--quenchers, resulting in fluorescence recovery of the sensing paper. The developed paper-based sensor performed well in the one-step detection of the SARS-CoV-2 S1 protein with a detection limit of 0.067 ng/mL (0.335 pg/test). The assay exhibited good selectivity and anti-interference in the detection of the SARS-CoV-2 S1 protein in artificial saliva. Moreover, the paper-based aptasensor was successfully used to detect the SARS-CoV-2 S1 protein in actual environmental samples with recoveries of 90.87–100.55% and relative standard deviations of 1.52–3.41%. The proposed technology provides a cost-effective alternative to traditional antibody test strips for a wide range of POC diagnostic applications. Full article
(This article belongs to the Special Issue Point-of-Care Biosensors: Design and Applications)
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