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Application of Nano-Materials Technology and Raman Spectroscopy in Biochemical Sensors

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Optical Sensors".

Deadline for manuscript submissions: 25 July 2025 | Viewed by 169

Special Issue Editors


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Guest Editor
Precision Medical Single Molecule Diagnosis Technology Research Center, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, Chongqing 400714, China
Interests: constructing a biochemical sensor based on nanopore single molecule technology and raman spectroscopy for life science and environmental detection research

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Guest Editor
Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China
Interests: application and research of super-resolution optics in biomedical field

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Guest Editor
College of Life Science, Hunan Normal University, Changsha 410081, China
Interests: biosensing; molecular sensing; disease diagnosis; environmental monitoring

Special Issue Information

Dear Colleagues,

Nanomaterials, with their unique physicochemical properties, hold immense potential in the field of biochemical sensors. They enhance sensor sensitivity and selectivity, improving signal transmission efficiency. Metal nanostructures, such as gold and silver nanoparticles, are widely used in biomolecule detection due to their Surface-Enhanced Raman Scattering (SERS) effect, enabling the high-sensitivity analysis of disease biomarkers. SERS technology offers in situ, non-destructive detection, fingerprint identification, and single-molecule level analysis, which is crucial for bio-chem sample analysis. SERS biochemical sensors typically qualitatively and quantitatively analyze target analytes based on changes in Raman-tagged molecular signals, encompassing target recognition and capture, the preparation of SERS substrates, and signal reading. Researchers have conducted outstanding studies in nanomaterial fabrication, probe design, and Raman spectral signal reading and analysis. The combination of nanomaterial technology and Raman spectroscopy in biochemistry sensing provides a new analytical method with high sensitivity and selectivity for biomolecule detection. With advancements in nanotechnology and Raman spectroscopy, SERS applications in the biosensing and imaging analysis of biomarkers, tumor cells, and microorganisms will become more extensive.

Dr. Wanyi Xie
Dr. Mingjie Tang
Dr. Weitao Huang
Guest Editors

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Keywords

  • nanomaterials
  • nanotechnology
  • Raman spectroscopy
  • SERS
  • biochemical sensors

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Published Papers (1 paper)

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Research

14 pages, 6527 KiB  
Article
Thickness-Tunable PDMS-Based SERS Sensing Substrates
by Diego P. Pacherrez Gallardo, Shu Kawamura, Ryo Shoji, Lina Yoshida and Binbin Weng
Sensors 2025, 25(9), 2690; https://doi.org/10.3390/s25092690 - 24 Apr 2025
Viewed by 148
Abstract
Surface-enhanced Raman scattering (SERS) spectroscopy is an ultra-sensitive analytical method with the powerful signal-molecule detection capability. Coupling with the polydimethylsiloxane (PDMS) material, SERS can be enabled on a polymeric substrate for fast-developing bio-compatible sensing applications. However, due to PDMS’s high viscosity, conventional PDMS-SERS [...] Read more.
Surface-enhanced Raman scattering (SERS) spectroscopy is an ultra-sensitive analytical method with the powerful signal-molecule detection capability. Coupling with the polydimethylsiloxane (PDMS) material, SERS can be enabled on a polymeric substrate for fast-developing bio-compatible sensing applications. However, due to PDMS’s high viscosity, conventional PDMS-SERS substrates are typically thick and stiff, limiting their freedom for engineering flexible micro/nano functioning devices. To address this issue, we propose to adopt a low viscosity decamethylcyclopentasiloxane (D5) solvent as a diluent solution. Via controlling the mixture ratio of D5 and PDMS and the spin-coating speed for deposition, this method resulted in a film of a well-defined thickness from sub-millimeter down to a 100 nm scale. Furthermore, thanks to the unsaturated Si-H chemical bonds in the PDMS curing agent, the PDMS film could effectively reduce the Ag+ ions to Ag nanoparticles (NPs) directly bonding onto the substrate surface uniformly. Via adjusting the size and density of the AgNPs through reaction temperature and time, strong SERS was achieved and verified using R6G with the detection limit down to 0.1 ppm, attributed to the AgNPs’ plasmonic enhancement effect. Full article
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