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Fluorescent and Colorimetric Sensors for Diagnostics and Drug Analysis

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Chemical Sensors".

Deadline for manuscript submissions: 31 July 2025 | Viewed by 309

Special Issue Editor


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Guest Editor
Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Banchi di Sotto 55, 53100 Siena, Italy
Interests: cyclic voltammetry; electrochemistry; electrophoresis; agarose gel electrophoresis; affinity chromatography; voltammetry; electrochemical analysis; ELISA; nanomaterials
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Special Issue Information

Dear Colleagues,

Fluorescent and colorimetric sensors are powerful tools in diagnostics and drug analysis, offering sensitive and selective detection of biological and chemical analytes. Fluorescent sensors rely on changes in fluorescence intensity, wavelength, or lifetime in response to a target molecule, providing high sensitivity and real-time monitoring capabilities. Colorimetric sensors, on the other hand, induce visible color changes upon interacting with a target, making detection simple and cost-effective, often without the need for complex instrumentation. Both sensor types are widely used in medical diagnostics, environmental monitoring, and pharmaceutical analysis, aiding in the rapid, accurate identification of disease markers, pathogens, and drugs. Their versatility and ease of use make them essential in modern healthcare and research applications.

Dr. Mariagrazia Lettieri
Guest Editor

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Keywords

  • fluorescent sensors
  • colorimetric sensors
  • diagnostic applications
  • drugs analysis
  • bioanalytical assays

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Published Papers (2 papers)

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Research

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12 pages, 2722 KiB  
Article
Prediction of Microvascular Adaptation to Hypoxia Based on Myogenic Microcirculation Oscillations
by Andrzej Marcinek, Joanna Katarzynska, Artur Stanek and Jerzy Gebicki
Sensors 2025, 25(9), 2751; https://doi.org/10.3390/s25092751 - 26 Apr 2025
Viewed by 135
Abstract
Microcirculatory oscillations known as flowmotion are a recognized feature of blood flow that reflect the functional state of the vascular system. Many diseases are associated with impaired flowmotion, especially diseases that are accompanied by hypoxia. Low-frequency myogenic oscillations (0.052–0.15 Hz) are an important [...] Read more.
Microcirculatory oscillations known as flowmotion are a recognized feature of blood flow that reflect the functional state of the vascular system. Many diseases are associated with impaired flowmotion, especially diseases that are accompanied by hypoxia. Low-frequency myogenic oscillations (0.052–0.15 Hz) are an important regulator of microvascular adaptation to hypoxia. Here, we study the myogenic component of flowmotion using the FMSF–PORH (Flow Mediated Skin Fluorescence–Post Occlusive Reactive Hyperemia) technique. Myogenic oscillations were strongly activated under hypoxic conditions caused by occlusion of the brachial artery or intermittent hypoxic treatment. A strong correlation was noted between the hypoxia sensitivity parameter HS (the intensity of myogenic oscillations activated by hypoxia) and the normoxic myogenic flowmotion parameter VM (the intensity of myogenic oscillations under normoxic conditions). If HS is considered as a direct measure of the microcirculation response to hypoxia, then VM can be considered a measure of the microcirculation’s readiness to provide this response. The predictive value of the VM parameter is presented. The assessment of myogenic activity under normoxia conditions could thus provide a simple and rapid diagnostic tool for health care practitioners. Full article
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Review

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17 pages, 993 KiB  
Review
Fluorometric and Colorimetric Biosensors for the Assay of Cholinesterase Inhibitors
by Miroslav Pohanka
Sensors 2025, 25(9), 2674; https://doi.org/10.3390/s25092674 - 23 Apr 2025
Viewed by 212
Abstract
Cholinesterases, specifically acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), play critical roles in neurotransmission and are key targets for inhibitors with therapeutic and toxicological significance. This review focuses on the development and application of fluorometric and colorimetric biosensors for the detection of cholinesterase inhibitors. These [...] Read more.
Cholinesterases, specifically acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), play critical roles in neurotransmission and are key targets for inhibitors with therapeutic and toxicological significance. This review focuses on the development and application of fluorometric and colorimetric biosensors for the detection of cholinesterase inhibitors. These biosensors take advantage of the unique properties of AChE and BChE to provide sensitive and selective detection methods essential for environmental monitoring, food safety, and clinical diagnostics. Recent advances in assay techniques, including the use of gold nanoparticles, pseudoperoxidase nanomaterials, and innovative enzyme–substrate interactions, are highlighted. This review also discusses challenges and future directions for optimizing these biosensors for practical applications, emphasizing their potential to enhance public health and safety. Full article
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